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The difference of serological responses between naturally infected, experimentally infected, and vaccinated cattle with Brucella abortus strain 19 vaccine Sudibyo, Agus
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 2 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.45 KB) | DOI: 10.14334/jitv.v1i2.21

Abstract

The purposes of this research were to observe serological response differences of brucellosis between naturally infected, experimentally infected, and vaccinated cattle with Brucella abortus strain 19 vaccine . Identification of naturally infected cattle with B. abortus was carried out bacteriologically on milk samples collected from sero-positive brucellosis, while to determine serological responses blood samples were collected with 2 week interval from naturally and experimentally infected, and vaccinated cattle . Futhermore, the collected serum was examined serologically by using complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA, indirect and competitive) . The result indicated that antibody titres (ELISA unit) of vaccinated cattle reached the peak at week -6 . This antibody titre was relatively lower and decreased quicker than naturally or experimentally infected cattle.
Epidemiological study of brucellosis and its effect on reproduction failures in dairy cattle in DKI Jakarta Sudibyo, Agus
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 1 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.858 KB) | DOI: 10.14334/jitv.v1i1.6

Abstract

The purposes of this research were to observe prevalence, distribution and reproduction failure evidence of dairy cattle brucellosis in DKI Jakarta . To determine the group of cattle infected with Bntcella abortus, senim and milk samples were carried out . At the beginning, bulk of milk samples were collected from containers . Futhermore, the blood samples were simple randomly collected on cattle that were suspected positive brucellosis in the milk ring test . Those serum samples were tested by using rose bengal plate and complement fixation test . Questioner data in relation with on any reproductive failures, such as abortion, premature dead and born weak, were gathered from farmer within 8 consecutive month period . The result indicate that screening of brucellosis using milk ring test based on bulk milk sample was effective tools to determine the infected group of cattle . The average prevalence of brucellosis in DKI Jakarta area was 4 .5 %. Distribution of brucellosis prevalence were 8 .5% at Setiabudi, 2 .3% at Mampang Prapatan, and 2 .9% at Pasar Minggu . Brucellosis was found at least 201 cattle from 38 dairy cattle farm in DKI Jakarta . Brucellosis was caused abortion about 62 .5% dairy cattle and this value was significantly different than that of uninfected cattle (P<0.05) . Brucellosis also cause stillbirth around 9 .8% and born weakness 15 .2% . The widely distributed and high prevalence brucellosis of dairy cattle in DKI Jakarta was influenced by uncontrol of cattle movement and high abortion cases rate .
Studi Tentang Persepsi, Praktek dan Sikap terhadap Sistem HACCP pada Industri Pakan Dalam Mencapai Kesesuaian Keamanan Pakan Sudibyo, Agus; Yulistia, Aan
JURNAL STANDARDISASI Vol 13, No 3 (2011): Vol. 13(3) 2011
Publisher : Badan Standardisasi Nasional

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Abstract

industry have not yet been implementing the feed safety system including the feed hygiene practices. Therefore,A study of perceptions, practices and attitudes on hazard analysis critical control point (HACCP) in achieving feedsafety compliance of Indonesian stock-feed production has been conducted. This study was carried out usingframe works of study as follows, i.e. Module I, Assessment on existing conditions using methods of survey,observe, interview, plant visit and distribute a questionnaire to medium and large feed industries at 6 provinces,i.e Lampung, Banten, West Java, Center of Java, East Java and South Sulawesi with the number of samples forrespondents about 20 feed industries; They were also asked about the feed hygiene practices in their business,their systems used such as HACCP, and the perceptions and attitudes toward a range of feed hygiene issues;Module II, Identification of potential critical control points; Module III, Evaluation of HACCP programs. Theanalysis was conducted using descriptive analysis method that developed by Hair et al. and to test the differenceusing one-way statistical analysis of variance. The results showed that the HACCP systems were implemented at95% in the large feed industries and 65% in the medium feed industry, respectively (P< 0.005); 30% medium and15% of large feed industry managers stated that their business represented a low-risk to feed safety. Higherlevels of feed hygiene qualifications among the industry managers and higher perceptions among managers ofthe risk of feed safety of the business were also significantly related to use HACCP in sectors (P<0.05). Sixpotential hazards were identified along the process to be controlled as critical control points (CCPs). Total cost forimplementing the system was around 1 billion and 400 millions rupiahs; while the time needed since designing upto fully operating the system with audit results fulfill the prerequisite programs and HACCP requirement was about7 months.
De-Soekarnoisasi Dalam Wacana Resmi Orde Baru: Kilas-Balik Praktek-Praktek Rekayasa Kebenaran dan Wacana Sejarah Oleh Rejim Orde Baru Sudibyo, Agus
Jurnal Ilmu Sosial dan Ilmu Politik Vol 2, No 1 (1998)
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Gadjah Mada

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Abstract

New Order regime under Soeharto carried various ways out in order to weaken its rivals position. One of them is symbolical way, by using its formal discourses to create illegitimate and unfavourable construction on its rivals. This symbolical one is also applied to Bung Karno, the first President of Indonesia. Instead of less effectively legal-formal mechanisms to erode Bung Karnos influences, they delegitimated Bung Karnos in symbolical contex. This article looks that delegitimation practices to Bung Karno, or De-Soekarno-ization, is not only conducted on the level of manifest, for example by swept away Soekarnos loyalists fivm bureaucracy and military, but also on the level of symbolical-latent by negating and denying Bung Karnos historical roles and contributions. It occured in textual education books and government medias and on the various debate in mass media when apparatuses of New Order regime discreditted Bung Karno
Masyarakat Warga dan Problem Keberadaban Sudibyo, Agus
Jurnal Ilmu Sosial dan Ilmu Politik Vol 14, No 1 (2010): Multikulturalisme dan Pergulatan Identitas
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Gadjah Mada

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Abstract

The emergence of civil society in Indonesia has always been located in a framework to deal with such a strong state hegemony. Civil society is located as the antithesis to the state. This article argues that civil society in Indonesia is also facing the threat of the existence of civil society itself, so uncivil society emerged as a threat to civil society that comes from civil society itself. In addition, a serious threat to civil society also emerged from the stronger market intervention.
Wacana Penjarahan Dan Kekerasan Simbolik Terhadap Petani Sudibyo, Agus
Jurnal Ilmu Sosial dan Ilmu Politik Vol 2, No 3 (1999)
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Gadjah Mada

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Abstract

Perlawanan kaum petani sudah lama terjadi dalam konteks sejarah hubungan antara si kaya dan si miskin dan antara kaum petani dan negara. Persoralannya selalu bahwa negara, aparat keamanan dan pemilik modal telah mengambil alih secara paksa surplus ekonomi dari petani. Mereka bekerja sama dalam aksi penyerobotan tanah petani, pendirian perkebunan-perkebunan dan dalam menghadapi gerakan protes petani. Negara bukan hanya menghadapi gerakan perlawanan petani dengan represi-represi fisik. Secara sistematis negara juga melakukan propaganda dengan menciptakan gambaran-gambaran yang unfavourable tentang petani pelaku penjarahan dalam berbagai representasi wacana. Dalam batas tertentu, media massa justru memperkuat kecenderungan ini dengan menonjolkan dimensi kekerasan, pemberontakan dan anarkisme dalam mewacanakan aksi-aksi penjarahan petani.
Absennya Kajian Ekonomi Politik Media di Indonesia Sudibyo, Agus
Jurnal Ilmu Sosial dan Ilmu Politik Vol 4, No 2 (2000): Menggugat Kajian Media dan Kebebasan Pers
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Gadjah Mada

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Abstract

The rapid and deep changing of the media requires new approach in media studies in Indonesia. The cutently predominating positivism is inadequate. The article advocates the use of a critical political economy as an alternative approach to study media in Indonesia.
Epidemiological study of brucellosis and its effect on reproduction failures in dairy cattle in DKI Jakarta Sudibyo, Agus
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 1 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.858 KB) | DOI: 10.14334/jitv.v1i1.6

Abstract

The purposes of this research were to observe prevalence, distribution and reproduction failure evidence of dairy cattle brucellosis in DKI Jakarta . To determine the group of cattle infected with Bntcella abortus, senim and milk samples were carried out . At the beginning, bulk of milk samples were collected from containers . Futhermore, the blood samples were simple randomly collected on cattle that were suspected positive brucellosis in the milk ring test . Those serum samples were tested by using rose bengal plate and complement fixation test . Questioner data in relation with on any reproductive failures, such as abortion, premature dead and born weak, were gathered from farmer within 8 consecutive month period . The result indicate that screening of brucellosis using milk ring test based on bulk milk sample was effective tools to determine the infected group of cattle . The average prevalence of brucellosis in DKI Jakarta area was 4 .5 %. Distribution of brucellosis prevalence were 8 .5% at Setiabudi, 2 .3% at Mampang Prapatan, and 2 .9% at Pasar Minggu . Brucellosis was found at least 201 cattle from 38 dairy cattle farm in DKI Jakarta . Brucellosis was caused abortion about 62 .5% dairy cattle and this value was significantly different than that of uninfected cattle (P<0.05) . Brucellosis also cause stillbirth around 9 .8% and born weakness 15 .2% . The widely distributed and high prevalence brucellosis of dairy cattle in DKI Jakarta was influenced by uncontrol of cattle movement and high abortion cases rate . Keywords : Brucellosis, epidemiology, dairy cattle, DKI Jakarta
The difference of serological responses between naturally infected, experimentally infected, and vaccinated cattle with Brucella abortus strain 19 vaccine Sudibyo, Agus
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 2 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.45 KB) | DOI: 10.14334/jitv.v1i2.21

Abstract

The purposes of this research were to observe serological response differences of brucellosis between naturally infected, experimentally infected, and vaccinated cattle with Brucella abortus strain 19 vaccine . Identification of naturally infected cattle with B. abortus was carried out bacteriologically on milk samples collected from sero-positive brucellosis, while to determine serological responses blood samples were collected with 2 week interval from naturally and experimentally infected, and vaccinated cattle . Futhermore, the collected serum was examined serologically by using complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA, indirect and competitive) . The result indicated that antibody titres (ELISA unit) of vaccinated cattle reached the peak at week -6 . This antibody titre was relatively lower and decreased quicker than naturally or experimentally infected cattle. Key words : Brucellosis, naturally infected cattle, vaccinated cattle, serological response
The comparative study of antigenic protein characters of field isolates Brucella abortus cells with electrophoresis and immunoblotting Techniques Sudibyo, Agus; Pasaribu, Facriyan H; Wibawan, I.W.T.; Setiawan, Endhie
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 3 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.656 KB) | DOI: 10.14334/jitv.v1i3.32

Abstract

Live vaccine of Brucella abortus strain 19 has been used for prevention and eradication of brucellosis in cattle . The information of the use of this vaccine in Indonesia is still limited, while the effectivity, bacteriological and serological aspects of the vaccine are not much evaluated yet. The objective of this research is to study the differences of protein cell wall antigenicity profiles between B. abortus strain 19, strain 544 and field isolates. Protein cell wall was prepared by sonicafion of B. abortus S19, S544 and B. abortus field isolates biotype l, 2 and 3 . Antiserum against these B. abortus was prepared in cattle . Futhermore, the distribution of protein band was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), while protein antigenic profiles was examined by immunoblotting . The result showed that there was no significant different amongs protein cell wall of these B. abortus. From the antigenicity profiles exhibited that cattle vaccinated with B. abortus S19, no antibody was detected against protein less than 30 kDa. The other side natural infected or experimental infected cattle with B. abortus biotype 1 field isolate, antibody was detected until protein which has molecular weight about 15 kDa. Key words: Brucella abortus, antigenic protein, immunoblotting