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Phage FR38 Treatment on Sprague Dawley Rat Inferred from Blood Parameters and Organ Systems

HAYATI Journal of Biosciences Vol 19, No 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The ability of phage FR38 to lysis indigenous Salmonella P38 from feces of diarrheal patient has been studied. However, effects of phage FR38 on organ system were not revealed as yet. This study was conducted to observe the effect of phage FR38 on blood chemistry, kidney functions, and liver functions. Twelve Sprague-Dawley rats were used as a model for this study that were divided into two groups; (i) control and (ii) treated group with phage FR38. For treated phage group, each rat was administered by 5 ml/kg bw of 1.59·107 pfu/ml of phage intragastric. The blood parameters were analysed on day 16. The results revealed that body and organs weight, erythrocyte, hematocrit, hemoglobin, leukocyte, total protein, creatinine, SGOT, and SGPT of phage treatment rats were not significantly different with the control rats on day 16 (P > 0.05). Therefore, this study showed was no effect of phage FR38 on body weight, blood chemistry, kidney and liver functions of the rat (P > 0.05).

ISOLASI Campylobacter DARI KARKAS AYAM MENGGUNAKAN METODE KONVENSIONAL DAN POLYMERASE CHAIN REACTIONS [Isolation of Campylobacter from Poultry Carcasses using Conventional and Polymerase Chain Reaction Methods]

Jurnal Teknologi Dan Industri Pangan Vol 24, No 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

ABSTRACT Campylobacter jejuni and Campylobacter coli are two spesies of Campylobacter sp. frequently found as pathogenic bacteria causing human gastrointestinal infections. Contaminated chicken carcasses have been reported as the source of human campylobacteriosis. In this study, Campylobacter were isolated from chicken carcasses sold in traditional markets and supermarkets. In traditional markets, chicken carcasses are sold without proper packaging or in an open space and stored at room temperature (25-30°C) for prolonged period allowing pathogenic bacteria to grow. While at supermarkets, chicken carcasses are openly displayed or enclosed in plastic wrappings and stored in a refrigerator (4-8°C). A total of 298 samples of chicken carcasses from traditional markets and supermarkets in the area of DKI Jakarta, West Java (Bogor and Sukabumi) and Central Java (Kudus and Demak) were collected. Isolation and identification using conventional and Polymerase Chain Reactions (PCR) methods were done to determine the prevalence of C. jejuni and C. coli contamination in poultry. The results showed that chicken carcasses sold in the sampling area, both traditional markets and supermarkets, were contaminated with C. jejuni and C. coli. The contamination rate of Campylobacter sp. in chicken carcasses sold in supermarkets, were 14.1% by conventional methods and 29.5% by PCR. This was higher than those in traditional markets, i.e. 5.7 and 12.1%, respectively. It is also confirmed that the prevalence for contamination of C. jejuni was higher than C. coli in 298 samples, i.e. 16.1% and 3.7% by conventional method and 23.5% and 18.1% by PCR method respectively. Keywords: Campylobacter coli, Campylobacter jejuni, poultry carcasses, supermarket, traditional market

Uji Patogenisitas Zoospora Kapang Lagenidium giganteum terhadap Larva Instar-2 Nyamuk Aedes aegypti Skala Laboratorium (PATHOGENICITY TEST OF ZOOSPORA LAGENIDIUM GIGANTEUM FUNGI AGAINST AEDES AEGYPTI LARVAE 2nd UNDER LABORATORY CONDITION)

Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Abstract

Dengue Haemorrhagic fever (DHF) is one of fearsome diseases in society. Incidence of the disease isincreasing. Dengue fever is caused by dengue virus and transmitted by Aedes aegypti mosquito vector.Various chemical controls have been conducted to prevent the spread of the disease, but active contents ofthe chemical controlling substances are suspected causing many negative effect, in environment, such asvector resistance, death of non target living creatures, and environmental contamination.This researchobjective was to find an alternative solution in order to control the dengue vector by using entomopathogenicfungi as biological control agent. This research was conducted by isolation and identification of fungiinfecting mosquito larvae. Macroscopic observation revealed that one of the nine isolation products wasLagenidium giganteum. The effectiveness test in laboratory showed the zoospore LD50 to Ae.aegypti larvaeof instar 2nd was 2,35 x 106 zoospore/ml, while the LD95 value was 1,35 x 107 zoospore/ml. The oosporeeffectiveness test showed LD50 was 6,7 x 102 oospore/ml and LD95 was 1,94 x 103 oospore/ml. Using LPCBdye and blue tolouidin 2,5%, the infection mechanism of L.giganteum fungi in Ae.aegypti mosquito larvawas detected. The research is concluded that the entomophatogen fungi L. giganteum was very prospectiveto be used as a biological control agent against vector of DHF.

Tingkat Insidensi Malaria di Wilayah Pemanasan Kelambu Berinsektisida Tahan Lama dan Wilayah Kontrol (MALARIA INCIDENCE RATE OF HEAT ASSISTED REGENERATION LONG LASTING INSECTICIDAL NETS AREA AND CONTROL)

Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Abstract

Long lasting insecticidal nets (LLIN) is one effective way to prevent malaria. Permethrin treatedLLIN is one type of LLIN which is recommended by WHO. Several studies have shown that these types ofLLIN requiring heat assisted regeneration after washing to enhance the biological activity of insecticidethat contained in the LLIN fibers. This study aimed to compare the incidence rates of malaria in childrenunder five years old who live in the intervention area (where the heat assisted regeneration on LLIN afterwashing was applied) and control area (where the heat assisted regeneration on LLIN after washing wasnot applied). Data of malaria cases was collected from laboratory log book at all health centers in BangkaDistrict, in the period of June June 2007 until July 2008. Data were analyzed with Poisson regressionmodels. The results showed that the incidence rate of malaria in children under five years old was notsignificantly different between the treatment and control areas.

Deteksi Mycobacterium Avium Subspesies Paratuberculosis pada Susu Pasturisasi yang Dijual di Bogor

Jurnal Veteriner Vol 11, No 2 (2010)
Publisher : Jurnal Veteriner

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Abstract

Mycobacterium avium subspesies paratuberculosis (MAP) is a thermal tolerant bacteria. The presenceof these bacteria in pasteurized dairy milk is associated with infectious bowel disease in human known asCrohn’s disease. The aim of this study was to detect MAP in pasteurized dairy milk sold in Bogor. Fourtytwo samples of plain flavoured milk (180–250 ml) from 7 producers were bought from supermarkets inBogor. The presence of MAP was detected by isolation and conventional polymerase chain reaction (PCR)using IS 900 and F57. Bacterial isolation were done by Herrold’s egg yolk medium with mycobactine J(HEYMj) and without mycobactin J (HEYM) and incubated at 37°C for 20 weeks. The DNA extraction ofall pasteurized dairy milk samples were conducted by DNeasy® Tissue Kit. Amplification conditionsfor PCR were: 1 cycle at 94°C for 10 minutes, 40 cycles at 94°C for 1 minute, 58°C for 1 minute, and72°C for 3 minutes, and 1 cycle at 72°C for 7 minutes. After 20 weeks of incubation, there were no sign ofMAP which grew in all isolation mediums. The PCR IS 900 and F57 did not detect the DNA band of thetarget. In the conclusion, there was no MAP detected in pasteurized dairy milk sold in Bogor.

The Application of Decomposting Microbes For Reduce Amonia and Hydrogen Sulfide Emission From The Piggeries in Bali

Media Veteriner Vol 6, No 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The unpleasant odor of piggeries is mostly caused by the emission of ammonia and hydrogen sulfide. An experimentwas conducted to study the effect of decomposting microbes EM4 on the emission of ammonia and hydrogen sulfide. The manure was treated with four different concentrations of EM4@, 0´70, 0.5%, 1.0% and 1.5% respectively. The ammonia released was trapped in 0.02N boric acid and analyzed with Nessler method, while the hydrogen sulfide was trapped in 0.02N zinc acetate and analyzed with the methylene blue method. The study suggested that the high concentrationof ammonia and hydrogen sulfide gas were decreasedafter 14 days, and there was no significant differencesin either ammonia or hydrogen sulfide emission although theammonia emission decreased to under 20 ppm with of 1.5%EM^" and the hydrogen sulfide decreased to 0.202 ppmwith application of 1.0% EM4@.

Milkchecker, As An Alternative Tool To Detect Subclinical Mastitis

Media Veteriner Vol 4, No 1 (1997): Media Veteriner
Publisher : Media Veteriner

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Abstract

Two hundred and thirty milk samples of different quarters taken from dairy cows around Bogor and Cipanas were used in this study. The milk samples were tested aseptically against subclinical mastitis using the MC and AMP in the field and in the laboratory. The amount of leucocytes were counted using the BREED method.Using the MC the sensitivity is 85.2% and the specificity is 97.8% in detecting subclinical mastitis. In comparing the use of MC to the AMP or to the BREED method no significant difference was found. The MC should be used routinely to detect mastitis especiallynew for cases of subclinical mastitis.

The IPB-I Reagent As An Alternative Tool To Detect Subclinical Mastitis

Media Veteriner Vol 5, No 1 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Mastitis subklinis hanya dapat dideteksi melalui pemeriksaan mikrobiologi dan penghitungan jumlah sel radang terhadap contoh susu. Penyakit ini sangat merugikan peternak karena produksi susu menurun dan seringkali berkembang menjadi mastitis klinik atau kronis yang berakibat penyingkiran sapi lebih awal. Melakukan deteksi dini dengan pereaksi terhadapcontoh susu dapat memperkecil resiko tersebut. Ujimastitis subklinis di lapang yang ada sampai saat ini,seperti Calijbrnia Mastitis Test (CMT), masih jarangdilakukan karena harga pereaksinya cukup lnahal dansulit diperoleh di pasaran. Untuk memperoleh suatuteknik yang cepat dan pereaksi untuk uji mastitis subklinis di lapang yang relatif lebih mudah, murah dan bahan-bahannya mudah diperoleh di pasaran, maka dikembangkanlah pereaksi yang diberi nama "IPB- I ". Dari penelitian ini diperoleh data bahwa sensitivitas IPB- I, CMT, Whiteside Test (WST), Aulendorfer Mastitis Probe (AMP) mod- 1 dan AMP mod-2 berturut-turut 0,99; 0,92; 0,94; 0,92 dan 0,94. Sedangkanspesifisitasnya berturut-turut 0,92; 0,37; 0,32; 0,47 dan0,89. Nilai prediksi (predictive valz~e)I PB- I, CMT, WST, AMP mod-1 dan AMP mod-2 berturut-turut 0,95; 0,99; 0,97; 0,99 dan 0,97. Nilai Keterhandalan IPB-I lebih tinggi dibandingkan dengan pereaksi lainnya. Namun nilai prediksi untuk pereaksi masih harus diperbaiki.

The Examination of Slaughtered Death Chicken Meat Quality Using the Biological Values

Forum Pasca Sarjana Vol 30, No 1 (2007): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

In recent years, there has been an increasing an abuse of slaughtered death chicken for human consumption, so it is important to find a practice ways to identify it. Experiments were conducted to determine whether the quality of meat taken from slaughtered death chicken can be detected through quality attributes of meat (Warner-Bratzler (WB) shear value, CIE L* a* b* color, and histological changes.  Thirty pieces of breast (M. pectoralis) and thigh (M. biceps femoris) meat were obtained from commercial slaughtering house.  The samples were classified into three groups namely halal slaughtered chicken (AHS), slaughtered death chicken (AMS), and slaughtered stressed chicken (ALS) and all samples processed for microscopic observations, for WB value and color after 1, 5 and 9 h postmortem (PM).  This study showed that percentage of degenerated and necrotic muscle fibres of breast and thigh meat of AMS and ALS were significantly higher (P0.05).  The L* value of breast and thigh meat of AMS and ALS were lower whereas a* value of breast and thigh meat of AMS were significantly (P

The prospect of Achehnese Traditional Foods as a Healthy Food: The Initial Detection of Antimicrobial Activity of Pliek u Oil and Pliek u Crude Extracts

Forum Pasca Sarjana Vol 32, No 1 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Antimicrobial activity of pliek u oil and pliek u crude extracts were evaluated against seven bacterial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella Enteritidis, Bacillus cereus, Pseudomonas aeruginosa, Pseudomonas fluorescens) and one fungal strain (Candida albicans).  Pliek u oil has been used as cooking oil and medicinal of skin diseases, wound, fever, headache and stomache.  Pliek u has been consumed as spices and sambal, and also used for feed poultry.  These foods collected from home industry at Redeup village in Aceh Besar, Nanggroe Aceh Darussalam.  The hexane and ethanol extracts of pliek u were obtained by standard method.  The antimicrobial activity was detected using paper disc method and the concentration of crude extract was determined by  dilution method.  Among antimicrobials extracts tested, the ethanol crude extract (EEP) was most active against all microbial strains.  The ethanol crude extract obtained from pliek u previously extracted by hexane (EERH) was active toward bacterial strains and only hexane crude extract was active against C. albicans.  The results indicated that ethanol crude extract (EEP) showed antimicrobial activity at a Minimal Inhibitory Concentration (MIC) and a Minimal Microbicidal Concentration (MMC) at 2.5-10 mg/ml and  10-20 mg/ml, respectively. EEP was still active at 100ºC, 121ºC for 15-60 minute, 28ºC (room storage), 10ºC (refrigerator temperature), both for 1-6 months and at pH from 1-11. The results of the research concluded that EEP showed significant antimicrobial activity.   Key words: pliek u, coconut fermentated, antimicrobial activity, toxicity test

Co-Authors . Andriani A. Winny Sanjaya Abdulwahed A. Hassan Abdulwahed Ahmed Hassan ADI SUDONO Agatha W. Sanjaya AGATHA WINNY SANJAYA AGUSTIN INDRAWATI Anastasia Winny Sanjaya Andriani . Andriani Andriani Andrijanto Hauferson Angi, Andrijanto Hauferson Annytha Ina Rohi Detha, Annytha Ina Rohi Anton Apriyantono Asep Saefuddin Betty Sri Laksmi Jenie Daowen Zhang Denny W. Lukman Denny W. Lukman Denny Widaya Lukman Denny Widaya Lukman DEWI SARTIKA Eko Sugeng Pribadi Etih Sudarnika Ewald Usleber Fadjar Satrija Feri Kusnandar Frans Umbu Datta Hadri Latif Harsi Dewantari Kusumaningrum Heinrich Enbergs Herastuti Sri Rukmini Heri Yulianto, Heri Herwin Pisestyani, Herwin I W.T. Wibawan I WAYAN TEGUH WIBAWAN Ida Bagus Arka Idwan Sudirman IETJE WIENTARSIH Jodi Vanden Eng KIBUUKA ROBERT L.I. Sudirman Lisdar I. Sudirman LISDAR I. SUDIRMAN Lubis, Muhammad Pauzi Mangaraja Pidoli Tampubolon Maya Purwanti Mazdani Ulfah Daulay, Mazdani Ulfah Ni Wajan Leestyawati palgunadi Nugraha, Arifin Budiman Nurliana . NURLIANA NURLIANA Patsiwi, Iccha Pradipta Purwanti Maya Raden Roso Soejoedono Rastina R, Rastina Razali . Retno Damayanti Soejoedono Rifda Naufalin Rismayani Saridewi, Rismayani Rita Kusriastuti Rochman Naim Sanjaya, A. W. Setyo Widodo SRI BUDIARTI Srihadi Agungpriyono Surachmi Setiyaningsih Surachmi Setyaningsih Suryono . Umi Cahyaningsih Upik Kesumawati Hadi W. Sanjaya, Angelina Wicaksono, Ardilasunu Widagdo S. Nugroho Widagdo Sri Nugroho Widagdo Sri Nugroho William A. Hawley Winiati P. Rahayu Zahid, Abdul