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POTENSITRICHODERMA SP. DANGLIOCLADIUM SP. SEBAGAI MIKROBA ANTAGONIS TERHADAPFUSARIUM OXYSPORUM F.SP.CUBENSE SUDARMA, I MADE
AGROTROP Vol. 1, No. 1 Mei 2011
Publisher : AGROTROP

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Potency of Trichoderma sp. and Gliocladium sp. as microbial antagonist on Fusarium oxysporum f.sp. cubense A total of 115 different fungus isolate were recovered from banana crop habitats with and without Fusarium wilt disease symptom. These were than assessed for their antagonist ability againstFusarium oxysporum f.sp.cubense (Foc)in vitro.The soil samples were collected from three regencies in Bali, i.e. Karangasem, Klungkung and Jembrana which are the main banana growing areas in Bali. Soil sampling was done in two sites in each regency representing the banana habitat with and without Fusarium wilt symptom, by collecting 100 grams of soil surrounding the banana plant at the depth of 20 cm. The isolate was macroscopically identified to know the color of colony and the growth rate, and microscopically to observe the shape of hypae, the shape of spore, and sporangiospore. The research was done on August until December 2009. Results indicated that one isolates ofGliocladium sp. and 22 isolates of Trichoderma sp. active against Foc. Trichoderma sp. was able to inhibit the Foc mycelium growth were 78.89 ± 1.11- 95.83 ± 7.22 % and Gliocladium sp. inhibited until 95.83 ± 7.22%. After tested with the Foc isolated from four banana cultivars in Bali (Saba, Ketip, Susu and Raja), all of the isolates was able to inhibit the Focin vitro.
LAJU INFEKSI PHYTOPHTHORA PALMIVORA PADA TANAMAN KAKAO DI DESA PULUKAN, JEMBRANA SUDARMA, I MADE
AGRITROP Vol. 24, No. 2 Juni 2005
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The aim of the research are to know infection rate of P. palmivora, relationship between height of fruits, age of fruits and disease intensity. Field experiment was carried out at Pulukan, Jembrana, started from November 2001 to January 2002. The observation were done to disease intensity and infection rate of P. palmivora that accured naturally by a weekly observation on 20 plants samples from 120 plants (40 are) of reasearch. The disease intensity in relation with height of fruits and age of fruits were analysed by using correlational method. The result of this experiment showed that epidemic of disease was developed as sigmoid curve. Infection rate maximum, 0,077 per unit per day on young fruits age one month. The height and age of fruits factors influenced development of disease. Relationship between height fruits from soil and disease intensity as, Y = 21,089 – 4,671 X1 (r = - 0,679**, r2 = 46 %, X1 = height fruit from soil) and relationship between age of fruit and disease intensity is Y = 20,257 – 4,339 X2 (r = -0,658**, r2 = 43 %, X2 = age of fruit).
DAYA HAMBAT EKSTRAK BIJI JINTAN PUTIH (CUMINUM CYMINUM) DAN BIJI PALA (MYRISTICA FRAGRANS) TERHADAP JAMUR PUTIH FUSARIUM OXYSPORUM F.SP. VANILLAE PADA MEDIA PDA DAN PEMBIBITAN TANAMAN PANILI SUDARMA, I MADE
AGRITROP Vol. 24, No. 4 Desember 2005
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Stem rot disease on vanilla caused by Fusarium oxysporum f.sp. vanillae has been known as the most destructive disease on vanilla in Bali since 1992. A drastic disease of vanilla cultivation areas and vanilla production occurred during 1991 to 2001 resulted from the disease. The vanilla cultivation areas has been decreased about 91% and the production of vanilla bean has also been decrease about 98% during that period. Many effort have been done to control the disease such as the use of synthetic fungicide, cultural practice, etc., but until now the disease is still can not be controlled appropriately. In order to find other alternatives for controlling the disease, seeds of plants were studied for their bioactivity against Fusarium oxysporum f.sp. vanillae. Of these, the seed extracts of C. cyminum and M. fragrans showed their inhibitory activity 77.35% and 62.13% against this fungus on potato dextrose agar (PDA) medium at concentration of 0.5% (w/v). On a green house experiment, application of the seed extract into the soil at the concentration of 0.5% (w/v) (in the process of seedling preparation) obviously inhibited the development of the population of this fungus, their inhibitory activity 99.9%. These result suggested that the seed extracts of Jintan Putih ( C. cyminum) dan Pala (M. fragrans) potentially can be used to control stem rot disease on vanilla.
PENGENDALIAN PENYAKIT BUSUK BUAH KAKAO DENGAN PESTISIDA NABATI SUDARMA, I MADE; SUPRAPTA, D. N.
AGRITROP Vol. 28, No. 1 Maret 2009
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Pod rot disease in Cacao (Theobroma cacao) caused by Phytophthora palmivora has been known to cause serious damage at cacao-growing area in the world. The disease can cause crop loss until 90%. Hitherto cacao farmer controls disease only on usage of synthetic pesticide. Therefore important is performed a operation research of disease with other alternative, like usage of leaf extract Piper betle and clove flowers (Eugenia caryophillata), as botanical pesticide. Research has been conducted in Pulukan village, Sub-Province Jembrana in January until April 2006, to study botanical pesticide effectivity (plant extract) to control pod rot disease. Research applies completely randomized block design, with four treatments and six replications, causing is required 24 experiment units. Every re-attempt unit thrice and ten tree pears observed as sample. Disease percentage is observed every one week once in each treatment. Results of the experiment indicated that leaf extract of P. betle and clove flowers effective controls pod rot disease (alone or in combination). The extract mixture P. betle and clove flower (combination) can suppress disease until 64,57 %, clove flowers extract (alone) 33,33% and synthetic pesticide (Dithane M45) equal to 60,40%. Result of this depicts treatment tested is usable to reduce pathogen pod rot disease of cacao.
UJI EFEKTIVITAS MIKROBA ANTAGONIS TERHADAP PATOGEN PENYEBAB BUSUK BUAH KAKAO SUDARMA, I MADE; SUSANTA WIRYA, I G. N. A.
AGRITROP Vol. 28, No. 3 September 2009
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Pod rot disease in cacao (Theobroma cacao) caused by Phytophthora palmivora has been loss until 90%. The major economic loss is from infection of the pod. Pods can be infected at any age, but most significant economic loss arises from infection during the two months prior to ripening. Pod infected at this stage can be a total loss because the fungus can easily pass from the pod husk to the seed-coat of the bean in a cacao still be convergent only at usage of synthetic pesticide. Therefore need to be searched other alternative to control the disease, namely by exploiting antagonist microbe. Research has been done in Laboratory of Microbiology and Microbial Pesticide, Department of Agroecotechnology, Faculty of Agriculture, Udayana University, Jl. PB. Sudirman Denpasar, from April until October 2007. There are three research phases done like : 1) insulation pathogen cause of disease, 2) antagonist microbe inhibitory activity test to growth of pathogen in vitro, and 3) inhibitory activity test free cell antagonist microbe to growth of pathogen in vitro. Result of research shows, found two species pathogen cause of pod rot disease of cacao namely Phytophthora sp. (A) and Phytophthora sp. (B). Based on result of examination out of four antagonist microbes tested like Trichoderma sp.; Saccharomyces sp.; Pseudomonas fluorescens and Bacillus sp., that is most effective in depressing pathogen is Trichoderma sp. with inhibitory activity equal to 83,98% to Phytophthora sp. (A) and 82,18% to Phytophthora sp. (B) at PDA media. Secondary metabolite released by fourth of antagonist microbe depress both pathogen cause of pod rot disease of cacao at PDA media.
APLIKASI POLYMERASE CHAIN REACTION-RIBOSOMAL INTERGENIC SPACER ANALYSIS (PCR-RISA) UNTUK MENENTUKAN KERAGAMAN MIKROBA TANAH PADA HABITAT TANAMAN PISANG DENGAN DAN TANPA GEJALA LAYU FUSARIUM Sudarma, I Made; Suprapta, Dewa Ngurah; Sudana, I Made; Maya Temaja, I Gede Rai
Bumi Lestari Journal of Environment Vol 12, No 2
Publisher : Udayana University

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A major problem in the soil microbiology is 99% soil microbes could not be grown in artificial media; therefore, to describe the true diversity of microbes in the soil must use a molecular approach. The approach was polymerase chain reaction-ribosomal intergenic spacer analysis (PCR-RISA). This method can be used for determining soil microbial DNA profile (the number of DNA bands). The soil samples were collected from three regencies in Bali,  i.e. Karangasem, Klungkung and Jembrana which are the main banana growing area in Bali. Soil sampling was done in two sites in each regency representing the banana habitat with and without Fusarium wilt symptom, by collecting 100 grams of soil surrounding the banana plant at the depth of 20 cm. The result of PCR-RISA showed that DNA profile of the soil microbes in the soil of banana habitat without Fusarium wilt symptom (HN) more than of the soil banana habitat with Fusarium wilt symptom (HF). DNA profiles at HN there were three, each of the indicated 450 bp, 250 bp and <100 bp, whereas in HF were two bands of DNA, each indicated as 250 bp and <100 bp. Based on the number of DNA bands proved that the soil microbial community in the HN is higher than in HF.
Hubungan Antara Keragaman Gulma dengan Penyakit Bulai pada Jagung (Zea mays L.) Stadium Pertumbuhan Vegetatif SUDARMA, I MADE; SUADA, I KETUT; AYU YULIADHI, KETUT
Agrotrop: Journal on Agriculture Science Vol 2, No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Weeds play an important role in the spread of downy mildew disease, competition, and lower cropyields of corn. Research using the survey method in 3 locations of Sanur village, Denpasar City. Eachlocation is taken 5 samples, and each sample area was 1m2, which was arranged diagonally. Forweeds identification was using reference i.e. Weeds of rice in Indonesia, and identification of downymildew disease using reference i.e. Maize Disease: A Guide for Field Indentification and microscopically,while the weeds diversity was determined based on Diversity Index of Shannon-Wiener. Relationshipbetween number of weed species and weed populations, and disease incidence were determined byregression and correlation analysis. Weed diversity on ecosystem of maize in vegetative stage is verylow with the diversity index of 0.779, this means weed ecosystem unstable, and dominated by a particularweed species was Paspalum commersonii Lamk. (Fam. Poaceae/Graminaceae) of 23.3%, with thedominance index of 0.709. Relationship between the number of weed species and disease incidence ofthe downy mildew disease was highly significant (P<0,01), while the relationship between weed populationand disease incidence of the downy mildew disease is not significant. Plant height and leaf number ofmaize was influenced by the type of weed, but weed populations only affect plant height of maize invegetative stage (V3-V11).
Analisis Harga Air untuk Irigasi Padi Sawah di Wilayah Perkotaan (Kasus Subak Pakel I dan Subak Pagutan Kota Denpasar) ADITYA, PUTU ANGGA; SUDARMA, I MADE; DJELANTIK, AAA WULANDIRA SAWITRI
E-Journal Agribisnis dan Agrowisata (Journal of Agribusiness and Agritourism) Vol.2, No.2, April 2013
Publisher : E-Journal Agribisnis dan Agrowisata (Journal of Agribusiness and Agritourism)

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ABSTRACT Water Value Analysis for Rice Irrigation in Urban Areas (Case Study of Subak Pakel I and Subak Pagutan Denpasar City) This study is analyzing the real price of water that must be spend by the rice farmer, the contribution of water for rice production by it coefficient elasticity and irrigation trouble that facing the rice farmer in city area, specifically in Denpasar city Bali province. This study uses the regression method and value of marginal product method to reach the water contribution by coefficient elasticity value and the water price. The result of study shows that water contribution to the rice production by coefficient elasticity is high. The water price that must be spend by the rice farmer by value of marginal product analysis is Rp 358.515/ha/season. The irrigation trouble that facing the farmer is irrigation way damage, garbage that obstruct water flowing, crab pest that discourage water in rice field, and the river damage that obstruct water flowing. The result of this study leads to the following recommendations. Firstly, the irrigation trouble must be fixed by the all subak member in united without expecting help from other stakeholder. Secondly, in the applying trouble fixing need a cost. The cost can be taken by implementation of the real water price by value Rp 358.515/ha/season. Keywords: rice, water price, co-efficient elasticity, irrigation trouble.
Bauran Pemasaran Pupuk Organik Bokashi Kotaku pada PT. Karya Pak Oles Tokcer Kota Denpasar, Provinsi Bali SIHOMBING, LELY RATNA; SUDARMA, I MADE; WIJAYANTI, UDAYANI
E-Journal Agribisnis dan Agrowisata (Journal of Agribusiness and Agritourism) Vol.2, No.2, April 2013
Publisher : E-Journal Agribisnis dan Agrowisata (Journal of Agribusiness and Agritourism)

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ABSTRACT The marketing mix is a combination of four variables which constitute the core of the company´s marketing system, consisting of product, price, place / distribution and promotion channels. This study aims to determine the marketing mix done by. PT. Karya Pak Oles Tokcer and constraints faced in marketing their products. Locations were selected by purposive sampling. The types of data collected included primary data and sekunde. Data collected by the method of observation, in-depth interviews and documentary study. Data were analyzed by descriptive kualitatf. Based on the survey results revealed that the marketing mix done by. PT. Karya Pak Oles Tokcer the product is, the company provides two different types of packaging in marketing their products to make it look more attractive in the eyes of consumers. For this type of regular use plastic packaging for the type of packaging sacks Kampil use. Bokashi fertilizer selling prices are determined based on a calculation of marketed raw material, labor, production, operating costs and other costs. Based on place / distribution channel, my town Bokashi fertilizer marketed through direct marketing distribution channels and indirectly. Promotional products through direct marketing activities (counter sales), sales promotion (rebates and bonuses) and advertising (brochures, newspapers, and radio from the company itself). Constraints faced by the company consists of internal and external constraints. Internal constraints in terms of the promotion of a less effective. External constraints in terms of competitors similar products. Keyword : Marketing Mix Pupuk Bokashi Kotaku.
Identifikasi Jenis dan Populasi Jamur Tanah pada Habitat Tanaman Kubis (Brassica oleracea L.) Sehat dan Sakit Akar Gada pada Sentra Produksi Kubis di Kecamatan Baturiti Tabanan INDRAYOGA, PANDE MADE; SUDARMA, I MADE; PUSPAWATI, NI MADE
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.3, Juli 2013
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Identify Types and Population of Soil Fungi on Cabbage (Brassicae oleracea L.) Health and Infected Habitat of Clubroot in Baturiti District, Tabanan Cabbage is a vegetable that potential to be developed, because a lot of people favored this vegetable. Cabbage crop production level is often affected by the attacks of pathogen Plasmodiophora brassicae that causes swelling at the root. This disease can reduce the production of cabbage plants up to 80%. The aim of this research is to investigate species variety and soil fungi population on healthy cabbage plant’s rizosphere (suppressive) and diseased cabbage or diseased sympton (conducive) of club root (Plasmodiophora brassicae), and also soil fungi which more dominant on isolation result of soil fungi, to investigate the relation of soil’s physico-chemistry nature with soil fungi population, and to investigate fungi species that able to antagonize P. Brassicae fungi. This research used 3 samples of soil which taken from both healthy and club root diseased cabbage plant’s rizosphere, where the soil’s sample taken from 3 locations, that are: Pekarangan, Baturiti Kaja, and Pacung. The result of this research shown that there are 17 species of soil fungi found on the sample of suppressive and conducivesoil in three locations, where the population of Fusarium sp. fungi becomes dominant soil fungi. This research also shown that on the relation between soil physico-chemistry nature and soil fungi population was not positively correlated, and species fungi of Trichoderma sp. is able to inhibit the growth of P. Brassicaefungi. Keywords: Cabbage plant, Plasmodiophorabrassicae, species and population of soil fungi, suppressive and conducive soil.
Co-Authors A.A ISTRI WINDA NOVITASARI, A.A ISTRI WINDA A.A.A WULANDIRA SAWITRI DJELANTIK A.A.A WULANDIRA SDJ, A.A.A A.A.A. WULANDIRA SAWITRI DJELANTIK, A.A.A. WULANDIRA SAWITRI AAA WULANDIRA SAWITRI DJELANTIK Abd. Rahman As-syakur Achsin, H.M. Aji Dedi Mulawarman Ariawan, Pasek Agus Arya Astawa, I Nyoman Gede Baiq Fadila Arlina, Baiq Fadila Cynthia Linaya Radiman, Cynthia Linaya D. N. SUPRAPTA DASI, SAUMIA KRISNA DEVI Dewa Ngurah Suprapta Dina Asnawati DJELANTIK, A.A. AYU WULANDIRA SAWITRI Duman Care Khrisne DWI WIDANINGSIH, DWI Elfis Umbu Katongu Retang, Elfis Umbu Katongu Emmy Yuanita FEBRYANA, NI PUTU VIVI FERDIANSYAH DWI MAULANA, FERDIANSYAH DWI GEDE WIJANA Gede Yudiana, I Made Hamdin, Candra Dwipayana Hilman Ahyadi Hilyatul Jannah I DEWA AYU SRI YUDHARI I G. N. A. SUSANTA WIRYA I Gede Rai Maya Temaja I Gede Setiawan Adi Putra I GUSTI NGURAH BAGUS I Gusti Ngurah Made Wiratama, I Gusti Ngurah I GUSTI NGURAH YURI PUTRA, I GUSTI NGURAH YURI I ketut Gede Darma Putra I KETUT RANTAU I KETUT SUADA I Made Adhika I Made Arsa Suyadnya, I Made I Made Mahadi Dwipradnyana, I Made I Made Narka Tenaya, I Made I Made Sudana I NENGAH ARI MEDIKANA, I NENGAH ARI I Nyoman Darmayasa, I Nyoman I NYOMAN GEDE USTRIYANA I Nyoman Wijaya I PUTU AJUS HERYANA, I PUTU AJUS I PUTU RIDIA PRAMANA, I PUTU RIDIA I Wayan Arthana I WAYAN BUDIASA I WAYAN SUANA I WAYAN WINDIA IDA AYU CANDRA DEWI, IDA AYU CANDRA Ida Ayu Dwi Giriantari IDA AYU LISTIA DEWI, IDA AYU LISTIA IGAA. AMBARAWATI Ismid Achmad, Ismid John Bremner KETUT AYU YULIADHI KETUT AYU YULIADHI KETUT BUDI SUSRUSA, KETUT BUDI KHAMDAN KHALIMI Kusuma, Yudha Manik LELY RATNA SIHOMBING Linawati, - Made Antara Made Ganesh Darmayanti, Made Ganesh MAHADIKARA, KADEK YARDI Maria Ulfa Mulyanto Mulyanto NI KETUT SUDARMINI, NI KETUT NI LUH ADE PEBRIANTARI, NI LUH ADE NI LUH KARTINI, NI LUH NI LUH WICANODIAN SURYA, NI LUH WICANODIAN Ni Made Puspawati NI NENGAH DARMIATI NI WAYAN NITA RAHAYU, NI WAYAN NITA NI WAYAN PUTU ARTINI NI WAYAN SUNITI NI WAYAN WINDA ARISANDI, NI WAYAN WINDA Nursetyohadi, Didik Nurul Ismilayli, Nurul Padmidewi, Desak Putu PANDE MADE INDRAYOGA Putra Suardika, Putra Putra, Indra Sukadiana Putri Nilakandi Perdanawati Pitoyo, Putri Nilakandi PUTU ANGGA ADITYA PUTU UDAYANI WIJAYANTI Rahmat Effendi, Rahmat Retang, Elfis Umbu Kantongu Ridwan Satrio, Muhammad Rizki Arifianto, Wahyu Rukmi Sari Hartati Saprini Hamdiani Sarkono Sarkono Sawitri Dj, AAA Wulandira SIDHARTA, NUR RANGGA Siti Raudhatul Kamali SOLICHATUN SOLICHATUN Sumichan, Andrew SWASTAWAN, I PUTU EDI Syamsul Alam Paturusi TORKIS JOEL SIMBOLON, TORKIS JOEL UDAYANI WIJAYANTI utami, Sinta wahyu WAYAN WINDIA Wijaksana, I Gusti Ngurah Winanda Windia, Wayan - WIRAWAN, IDA BAGUS MADE Yayuk Andayani YUNITA, EVA