Articles
34
Documents
KARAKTERISASI SENYAWA ANTIMUTAGENIK DARI TUMBUHAN PAKAN PRIMATA, PUSPA (Schima wallichii Korth.)

Biotika (Jurnal Ilmiah Biologi) Vol 2, No 2 (2003): Biotika Desember 2003
Publisher : Biotika (Jurnal Ilmiah Biologi)

Show Abstract | Original Source | Check in Google Scholar

Abstract

The objective of this research is to find compound which has antimutagenic activity on primate-consumed plant, Puspa (Schima walichii Korth). In this research, antimutagenic activity tested on tested animal, mice, with micronucleus test and phytochemistry to know active compound that involved in this activity. Antimutagenic activity test was conducted by giving induction material, cyclophosphamide (50 mg/kg), to mice to induct the mutation or mutagenesis. The result showed that methanol extract at 1000 dan 2000 mg/kg, decreased micronucleus frequency significantly than the negative control. To trace the active compound that role in antimutagenic activity, metanol extract fracted and the effect of each fraction tested by the same method. The result indicated that etil acetate fraction at 450 and 900 mg/kg or water fraction at 700 and 1400 mg/kg decreased micronucleus frequency significantly than negative control. Its means that the both of fraction has antimutagenic activity, and based on the decreased number is knew that water fraction is stronger than etil acetate fraction activity. Then, water fraction fracted with  n-butanol until n­-butanol and water fraction obtained. Both of those fraction at 350 and 700 mg/kg doses give antimutagenic activity significantly, but water fraction has antimutagenic activity is stronger (percentage of micronukelus decrease frequency at 42,0% for n­-butanol 700 mg/kg, and 63,3% for water fraction 700 mg/kg). From etil acetate fraction was isolated one compound and so is the n-butanol fraction. To know its antimutagenic activity, the compound was tested with micronucleus method. But both of this structure compounds has not been determined yet. Thereby, this result gives information that primate-consumed plant potency to get new medicine for human health.

Potensi Tumbuhan Erythrina (Leguminosae) sebagai Antifertilitas

Jurnal Kedokteran Maranatha Vol 7, No 2 (2008)
Publisher : Universitas Kristen Maranatha

Show Abstract | Original Source | Check in Google Scholar

Abstract

Erythrina plants (Leguminosae) were used as traditional antifertility agents. In the continuing research for novel plant with antifertility capacity from Indonesian plants, the methanol extract of Erythrina plants showed significant antifertility activity in an in vitro assay of spermatozoa of white rat (R. norvegicus). The methanol extract was separated using bioassay-guide n-hexane, ethylacetate, and n-buthanol fractionation. The ethylacetate fraction was tested in vitro on spermatozoa of white rat (R. norvegicus) at a concentration of 0.25 x 10-3 μg/μL. It showed a decrease of their motilities and viabilities, as well as an increasing abnormality of the spermatozoa.

An Isoflovonoid, Warangalone from the Stem Bark of Dadap Ayam (Erythrina variegata)

Jurnal ILMU DASAR Vol 9, No 1 (2008)
Publisher : Fakultas MIPA Universitas Jember

Show Abstract | Original Source | Check in Google Scholar | Full PDF (121.012 KB)

Abstract

In the course of our continuing search for novel paralytic compound from Indonesian plants, the methanol extract of the stem bark of Erythrina variegata (Leguminosae) showed significant paralytic activity against the third instar larvae of silkworm (Bombyx mori). The purposes of this research were isolation and structural elucidation of paralytic compound from the stem bark of E. variegata. Using the paralytic activity following the separation, the methanol extract was separated by combination of column chromatography to yield prenylisoflavone, warangalone. The chemical structure of warangalone was identified based on spectroscopic evidence and comparison with the previous reported. The paralytic activity of warangalone showed weak activity against the third instar larvae of silkworm (B. mori).Keywords: Erythrina variegata, Leguminosae, isoflavonoid, warangalone, paralytic activity

Aktivitas Antioksidan Minyak Atsiri dan Ekstrak Etanol Kulit Batang Sintok (Cinnamomum sintoc Bl.) terhadap 1,1-difenil-2-pikrilhidrazil (DPPH)

Indonesian Journal of Applied Sciences Vol 1, No 1 (2011)
Publisher : Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (718.443 KB)

Abstract

Sintoc (Cinnamomum sintoc Bl.) is a plant which is used as medicine. This plant has been known to have an analgesic antiinflamatory activity, therefore it is predicted to have an antioxidant activity. An investigation on antioxidant activity of sintoc essential oils and ethanolic extract of its cortex using ascorbic acid as standard has been carried out. Essential oils and ethanol extract of sintoc cortex was tested using DPPH (1,1-diphenyl-2-pikril-hidrazil) by measuring absorbance using visible spectrophotometer at 518 nm. The methods of this research were distillation of essential oils and extraction of sintoc cortex, determination of the essential oil and extract concentrations required for 50% inhibition of DPPH radical scavenging effect (IC50) with ascorbic acid as the possitive control. The variation concentration  of essential oils are 15, 5, 1, 0.1, 0.5 ppm and 25, 20, 17, 15, 10 ppm for ethanolic extracts. The results showed that the essential oil showed antioxidant activity with IC50 value was 16.29 ppm (5 times lower than ascorbic acid) and then ethanolic extract showed IC50 value 38.89 ppm (11 times lower than ascorbic acid, IC50 of ascorbic acid was 3.35 ppm).

In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant

Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Original Source | Check in Google Scholar

Abstract

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro  test  towards  breast  cancer  cell  T47D.  In  the course  of  our  continuing  search  for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of  E. variegata  showed  significant  anti-cancer  activity  against  breast  cancer  cell  T47D  in  vitro  using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure  of  an  anti-cancer  compounds  were  determined  on  the  basis  of  spectroscopic evidence  and  comparison  with  the  previously  reported  and  identified  as  an  erythrina  alkaloid (1)  and  isoflavonoid  (2).  Compounds  (1-2)  showed  anti-cancer  activity  against  breast  cancer cell  T47D  used  with  IC50  of    1.0  and  3.3  µg/mL,  respectively.  This  results  strongly  suggested that E. variegata is promising sources for anti-cancer agents.Keywords: Anti-cancer, Erythrina variegata, Leguminoseae

PENANDAAN DAN BIODISTRIBUSI RADIOFARMAKA TERAPI KANKER OTAK 1251-NIMOTUZUMAB TERHADAP MENCIT NORMAL

Jurnal Radioisotop dan Radiofarmaka Vol 13, No 2 (2010): Jurnal PRR 2010
Publisher : Jurnal Radioisotop dan Radiofarmaka

Show Abstract | Original Source | Check in Google Scholar

Abstract

ABSTRAKPENANDAAN DAN BIODISTRIBUSI RADIOFARMAKA TERAPI KANKER OTAK 125I-NIMOTUZUMAB TERHADAP MENCIT NORMAL. NIMOTUZUMAB adalah anti bodi monoclonal yang diketahui berperan banyak dalam antiproliferasi, proapoptisis dan efek antiangionik pada terapi kanker  otak  (glioma). Penandaan antibody monoklonal nimotuzumab dengan radiosiotop 1251yang memancarkan e1ektron auger telah dilakukan dengan menggunakan metode  iodogen. Hasil penelitiaan kemurnian radiokimia terbaik (97%)  menunjukan  mol  ratio protein (nimotuzumab) dengan kalium iodida  dan  lodogen adalah 1: 2: 1200  pada fraksi ke 6.  Kemurnian radiokimia diuji dengan  kromatografi kertas dengan fase diam  dan  fase gerak  masing –masing  adalah  kertas whatman  no. 1 dan  etanol-butanol-amonium  hidroksida dengan  perbandingan  3 : 2 : 1 Nilai Rf untuk  1251_nimotuzumab adalah 0,0 dan  125I adalah 0,9.  Hasil uji biodistribusi terhadap mencit normal selama 72 jam  menunjukan 125 I-nimotuzumab memiliki  waktu  paruh  yang  panjang  dan  terakumulasi  tinggi dalam organ  hati (1,97 ± 1,18%), organ ginjal (0,82± 0,28%)  dan  otot (0,61±,98%).  Sedangkan  akumulasi tertinggi  pada organ otak mencit normal (0,13 ±0,06%) baru terjadi setelah 24jam penyuntikan. Berdasarkan efek terapi dan akumulasi terhadap organ pada mencit normal, 1251_9imotuzumab berpotensi digunakan untuk terapi kanker otak. Kata kunci: Biodistribusi, Glioma, 1251,lodogen, Nimotuzumab ABSTRACT LABELING AND BIODISTRIBUSTION OF THERAPEUTIC RADIOPHARMACEUTICAL FOR BRAIN CANCER 125I – NIMOTUZUMAB IN NORMAL MICE. Nimotuzumab is a monoclonal antibody which known giving contribution in antiproliferation, proapoptosis and antiangionik effect on the therapy of brain cancer (glioma). The labeling of monoclonal antibody nimotozumab with 1251which radiate Juger electrons has been done with idogen method. The best result of radiochemical purity (97%) was shown ihI fraction 6 with the mol ratio of nimotuzumab towards potassium iodide and iodogen was 1 : 2 : 1200. Radiochemical purity was examined by paper chromatography with whatman paper no. 1 as the stationary dhase and ethanol-butanol-ammonium hydroxide with a ratio of 3 : 2 : 1 as the mobile phase. Rf Values for 1251-nimotuzumab is 0.0, while Rf value of 1251is 0.9. The biodistribution result on normal mice for 72 hours showed that 1251-nimotuzumab not only has a long half-life time but also has high accumulation in liver (1.97 e. 1.18(%0.)kidey (0.82±0.28%) and  muscle ( 0.61 ± 0.98 % ). The higest accumulation in liver (1.97 mice ( 0.128±0.06% ) occurred 24 hours after injection . Based on trhwe therapeutic effect and organ accumulation on normal mice,  1251-nimotuzumab could be potentially used for brain cancer therapy. Keywords: Biodistribution, Glioma, 1251,lodogen, Nimotuzumab      

SENYAWA BIOAKTIF DARI ERYTHRINA VARIEGATA (LEGUMINOSAE)

Berkala Ilmiah MIPA Vol 15, No 3 (2005)
Publisher : FMIPA UGM

Show Abstract | Original Source | Check in Google Scholar

Abstract

Dalam penelitian yang berkelanjutan ini bertujuan untuk menemukan senyawa bioaktif alami dari tanaman Indonesia, diperoleh hasil bahwa ekstrak metanol dari biji Erythrina variegata (Leguminosae) menunjukkan aktivitas paralitik dan daun E. variegata menunjukkan aktivitas antimalaria. Ekstrak metanol dipisahkan komponen-komponennya dengan kombinasi kolom kromatografi diperoleh dua senyawa aktif (1 dan 2). Struktur kimia senyawa aktif (1 dan 2) ditetapkan berdasarkan data-data spektroskopi dan perbandingan data dari senyawa yang berhubungan dari penelitian sebelumnya, dan diidentifikasikan sebagai alkaloid eritrina (1) dan turunan triterpenoid pentasiklik (2). Senyawa (1) memperlihatkan aktivitas paralitik terhadap instar ketiga ulat sutera (Bombyx mori) dengan ED50 15 μg/g diet. Senyawa (2) memperlihatkan aktivitas antimalaria dengan IC50 0,243 μg/mL terhadap pertumbuhan P. falciparum secara in vitro. Kata kunci: Erythrina variegata, Leguminosae, aktivitas paralitik, antimalaria

Analysis of compounds possessing inhibitory properties on mice locomotor activity from essential oils of Ki Lemo bark (Litsea cubeba lour. Pers)

INDONESIAN JOURNAL OF PHARMACY Vol 16 No 1, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (201.284 KB)

Abstract

Ki lemo barks has been used as traditional spasmolitic (called oils of massage) since a long time by ethnic of Dayak. The odors inhaled is presumed possessing inhibitory on mice locomotor activity. The present research has been done to determine the components in blood plasma of mice after inhalation of essential oil of ki lemo barks, and the possibility of their role in the inhibition on mice locomotor activity.Inhalation of essential oil of ki lemo bark (Litsea cubeba Lour. Pers) at the doses of 0.5 ml inhibit locomotor up to 60,75 %, whereas at the doses of 0.1 ml and 0.3 ml inhibit locomotor activity of 57,44 % and 54,20 %, respectively.Identification and quantification of volatile active compounds in the blood plasma were carried out by GC-MS analysis after half an hour, one hour, and two hours inhalation. The blood plasma of three mice were collected in heparin tube, isolated and concentrated by the C-18 column (100 mg-Seppak Waters) eluted with methanol and bidistilled water (60:40). The major compounds identified were citronellol, citronellal, α-terpineol, and 1,8-cineole. The blood level of those substances was increasing for one hour after inhalation but decreasing after two hours.Key words : essential oil of ki lemo bark (Litsea cubeba Lour. Pers), inhalation, locomotors.

The determination of quercetin in Plectranthus scutellarioides(L.) R.Br. leaves extract and its In SilicoStudy on Histamine H4 Receptor

INDONESIAN JOURNAL OF PHARMACY Vol 22 No 3, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (337.578 KB)

Abstract

Plectranthus  scutellarioides  (L.)  R.Br.,  or  jawer  kotok,  Family  Lamiaceae, grows  widely  in  Indonesia,  and  has  a  long  history  of  therapeutic  usage  in Indonesian traditional jamuto cure various diseases. The brownish purple leaves of  Plecranthus  contain  alkaloids,  saponin,  flavonoids,  tannin,  volatile  oils,  and quercetin  which  has  been  proven  to  exert  antiinflammatory  activity.  In  this research,  a  determination  of  quercetin  in  Plecranthus  leaves  extract  was performed and followed by a study of its interaction with histamine H4 receptor to  understand its  anti-inflammatory  activity.  The  dry  leaves  were  macerated by using  a  mixture  of  methanol  and  water  (1:1)  for  48  hours  and  the  solvent  was evaporated  at  low  temperature  (40-50oC).  Analysis  of  quercetin  in  the  extract was performed by using reversed-phase HPLC method LC-10AT VP (Shimadzu), Atlantis  Hilicsilica  C18  (Waters®)  150  mm  x  4.6  mm,  5  µm  as  stationary  phase and  a  mixture  of  acetonitrile,  phosphoric  acid,  and methanol  (40:50:10),  flow rate 0.8 mL/minute.  In silicostudy of quercetin with histamine H4 receptor was performed by using AutoDock Tools 3.0.5. Histamine H4 receptor (H4R) belongs to  G  protein-coupled  receptors  which  is  involved  in arthritis,  asthma,  and inflammations.  The  3D  structure  model  of  H4R  was  built  by  using  MODELLER 9v7.  Quercetin  contained  in  Plecranthus  leaves  extract  was  0.05  %.  This compound interacted with H4R viahydrogen bond formation with Lys158 (2.006 Å)  and  Glu182  (2.048  Å),  and  van  der  Waals  interaction  with  Trp90,  Leu91, Asp94, Tyr95, Phe168, Thr178, Ser179, Tyr319, Phe344, and Tyr340, therefore Plecranthus  leaves  extract  might  have  a  chance  to  be  used  as  histamine  H4 receptor inhibitor.Key  words  :   histamine  H4  receptor,  in  silico  study,  Plecranthus  leaves,  Plectranthus scutellarioides, quercetin

Efektivitas Lima Jenis Tanaman Sebagai Antivirus HIV (Human Immunodeficiency Virus)

Farmaka Vol 14, No 1 (2016): Supplement
Publisher : Fakultas Farmasi Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

AbstrakAIDS merupakan penyakit yang menyerang sistem kekebalan tubuh yang sampai saat ini belum banyak diketahui obat untuk menyembuhkan penyakit ini terutama dari bahan alam. Artikel ini mengulas tentang efektivitas senyawa obat yang diambil dari lima jenis tanaman sebagai obat antivirus HIV (Human Immunodeficiency Virus) dengan cara ekstraksi yang bertujuan sebagai pengembangan obat baru dari produk alami. Metode yang digunakan diperoleh berdasarkan pengumpulan data primer.. Dalam artikel ini data mengenai senyawa aktif dikumpulkan untuk mengetahui keefektifannya dalam menghambat proses replikasi virus HIV dengan hasil 5 jenis tanaman yaitu  yaitu Rhus Chinensis Mill, Camelia sinensis L, Cynomorium songaricum L, J. jurcas L, dan  Crataegus pinnatifida B.Kata kunci: HIV, Rhus Chinensis Mill, Camelia sinensis L, Cynomorium songaricum L, J. jurcas L, Crataegus pinnatifida B.AbstractAIDS is a disease that attacks the immune system which until now has not been widely known cure for this disease mainly from natural materials. This article reviews the effectiveness of drug compounds derived from five types of plants as antiviral drugs HIV (Human Immunodeficiency Virus) by extraction aimed developing new drugs from natural products. The method used was obtained based on primary data collection. In this article collected data on the active compound to determine its effectiveness in inhibiting the replication of the HIV virus with the results of five types of plants that are Rhus Chinensis Mill, Camelia sinensis L, Cynomorium songaricum L, J. jurcas L, and Crataegus pinnatifida B.Keywords: HIV, Rhus Chinensis Mill, Camelia sinensis L, Cynomorium songaricum L, J. curcas L, Crataegus pinnatifida B.