Articles

Recovery Gonad Betina dan Kontrol Produksi Sperma Secara Hormonal pada Ikan Heterobranchus longifilis Subagja, Jojo; Gustiano, Rudhy
Jurnal Akuakultur Indonesia Vol 4, No 2 (2005): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

Two experiments were conducted in the IRD-Gamet Laboratory, Montpellier, France. The first experiment was to evaluate the recovery of Heterobranchus longifilis females gonad after induced ovulation, under tropical condition.  Eggs diameter was used as indicator of the recovery rate following by induced breeding, hatching rate and abnormality of larvae. The results showed that the recovery was about 28 - 35 days after ovulation. The second experiment was carried out to improve milt production by hormone treatments, volume of intra testicular sperm and the numbers of spermatozoa were observed.  The results indicated that the treatmens did not prove any significant differences. Keywords: hormone, gonad, reproduction, Heterobranchus longifilis   ABSTRAK Dua kegiatan penelitian dilakukan di Laboratorium IRD-Gamet, Montpellier, Perancis.  Penelitian pertama dilakukan untuk mengetahui recovery gonad ikan Heterobranchus longifilis betina setelah ovulasi, di daerah tropis.   Diameter telur digunakan sebagai indikator recovery gonad, diukur pada saat sekitar 28-35 hari setelah ovulasi.  Penelitian kedua dilakukan untuk meningkatkan produksi sperma dengan memberikan perlakuan hormon.  Volume semen dan jumlah spermatozoa dievaluasi.  Hasil penelitian menunjukkan perlakuan tidak memberikan hasil yang signifikan. Kata kunci: hormon, gonad, reproduksi, Heterobranchus longifilis
Recovery Gonad Betina dan Kontrol Produksi Sperma Secara Hormonal pada Ikan Heterobranchus longifilis Subagja, Jojo; Gustiano, Rudhy
Jurnal Akuakultur Indonesia Vol 4, No 2 (2005): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.4.159-162

Abstract

Two experiments were conducted in the IRD-Gamet Laboratory, Montpellier, France. The first experiment was to evaluate the recovery of Heterobranchus longifilis females' gonad after induced ovulation, under tropical condition.  Eggs diameter was used as indicator of the recovery rate following by induced breeding, hatching rate and abnormality of larvae. The results showed that the recovery was about 28 - 35 days after ovulation. The second experiment was carried out to improve milt production by hormone treatments, volume of intra testicular sperm and the numbers of spermatozoa were observed.  The results indicated that the treatmens did not prove any significant differences. Keywords: hormone, gonad, reproduction, Heterobranchus longifilis   ABSTRAK Dua kegiatan penelitian dilakukan di Laboratorium IRD-Gamet, Montpellier, Perancis.  Penelitian pertama dilakukan untuk mengetahui recovery gonad ikan Heterobranchus longifilis betina setelah ovulasi, di daerah tropis.   Diameter telur digunakan sebagai indikator recovery gonad, diukur pada saat sekitar 28-35 hari setelah ovulasi.  Penelitian kedua dilakukan untuk meningkatkan produksi sperma dengan memberikan perlakuan hormon.  Volume semen dan jumlah spermatozoa dievaluasi.  Hasil penelitian menunjukkan perlakuan tidak memberikan hasil yang signifikan. Kata kunci: hormon, gonad, reproduksi, Heterobranchus longifilis
NILAI HETEROSIS DAN PERANAN INDUK PADA KARAKTER PERTUMBUHAN HASIL PERSILANGAN INTERSPESIFIK Tor soro DAN Tor douronensis [Growth Heterosis Values and The Role of Parent Tor soro and Tor douronensis in Interspesific Crossed] Radona, Deni; Subagja, Jojo; Kusmini, Irin Iriana; Gustiano, Rudhy
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2784.425 KB) | DOI: 10.14203/beritabiologi.v15i2.2394

Abstract

Fish Tor is one of potential fish commodities to be developed because of their economic value. This on experiment was designed to evaluate the effect of male and female parent on morphological characters i.e length and weight growth and the value of heterosis from crosses of Tor soro and Tor douronensis. Crosses were performed in both directions (reciprocal) to form four populations. Observations lenght, weight gain, specific growth in length, specific growth in weight and value of heterosis were carried out for two months (January-March, 2014). The results showed an increase of  length, weight, specific growth in weight, specific growth in length occurred in seed produced from parent male of T. soro and female of T. douronensis with values of 0.54 cm, 0.09 g, 0.65 % and 3.79 %, rescpectively. Crossed of ? T. douronensis x ? T. soro yield heterosis value (partial) of length (40.90%) and weight (116.66%) was higher than crossed of ? T. soro x ? T. douronensis  that only have heterosis value of 0 and 33.33 %, and heterosis value (reciprocal) on the length of 20.45 % and the weight of 45.22 %.
Aspek Ekologi Ikan Kancera (Tor soro) Kuningan dan Pematangan Gonad Melalui Implantasi Hormon Gonadotropin (HCG) Subagja, Jojo; Sulhi, M.; Asih, Sidi; Haryono, Haryono
JURNAL BIOLOGI INDONESIA Vol 5, No 3 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.478 KB) | DOI: 10.14203/jbi.v5i3.3181

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ABSTRACTEcological Aspect and Gonadal Maturation of Tor soro by HCG peletted Implantation. Theexperiment was conducted to determine optimal dosage of Gnrh-a hormon at gonadal maturationof mahseer from Kuningan (Tor soro). The fish was reared in concrete tanks (60m2). Thehormon was used for implantation is HCG (pregnyl). The different dosage of hormon are 250Iu/Kg-1 ; 500 Iu/Kg-1 ; 750 Iu/Kg-1 . The result of this reaserch showed that egg development isMei-Juni (average of egg diameter is 1,4 mm) and Januari (average of egg diameter is 1,35 mm),Other month, the egg diameter was not develop or atresia. Treatment with 500 Iu/Kg-1 dossageshowed the best effect on oosit diameter was at 21 day and 63 day from first oosit diameter of0,9 mm to 1,4 mm.Keywords: Seasonal changes, gonadal maturation, implantation, kancera (Tor soro)
Pertumbnhan Ikan Tambra (Tor tambroides) dan Kancera (Tor soro) Pada Proses Domestikasi dengan Jenis Pakan yang Berbeda Haryono, Haryono; Subagja, Jojo
JURNAL BIOLOGI INDONESIA Vol 4, No 3 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i3.3261

Abstract

ABSTRACTThe Growth of Tarnbra fish (Tor tambroides) and Kancera fish (Tor soro) inDomestication Process with Different Fed. The experiment was conducted toevaluate the growth of two mahseer (tambra and kancera) from Muller Mountainareas in Central Kalimantan which were different fed in aquarium i.e: A. Frozenworm, B. Fish feed, and C. shrimp feed. The experiment follows Randomized CompleteDesign Methods. The replication of treatment is three times, each aquarium filled with1 individual as treatment and replication. They were fed daily for 100 days andmeasured each 20 days. The results showed shrimp feed gave growth fastest (0.460gldays), and the lowest is 0.170 gldays; the growth oftambra fish faster than kancerafish. The water quality of aquarium is suitable for growing these fish.Kata kunci: Growth, Tambra fish (Tor tambroides), Kancera fish (Tor soro), Fed
PENINGKATAN PRODUKSI BENIH BAUNG {Mystus nemurus) MELALUI PERBAIKAN KADAR LEMAK PAKAN INDUK Suhenda, Ningrum; Samsudin, Reza; Subagja, Jojo
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (981.708 KB) | DOI: 10.14203/beritabiologi.v9i5.1991

Abstract

The production of good quality seed for culture purpose begins with proper attention to the nutritional requirements of the parental broodstock. The objective of this research was to know the level of lipid in broodstock feed to reach good seed production.The experiment was conducted in concrete ponds located at Research Instalation for Aquaculture Environment and Toxicology,Cibalagung, Bogor. Broodstock with average individual body weight of 420.8 ± 72.10 g were cultured in concrete pond 3.5 x 2.53x 0.8 m with stocking density of 15 fish/pond. Pelleted fish with 35% protein content and different lipid level of 4, 6, 8, 10, and 12 % were given with daily ratio 2 % of body weight. Gonadosomatic index, ovosomatic index; the weight, the number and the diameter of egg; fertilization rate, hatching rate, percentage of larva produced and daily growth rate and survival rate of 21 days old seed were observed. The result showed that there were no significant difference (P>0.05) among treatments for all parameters except for the diameter of the egg. The result showed that all of the broodstock were matured in three months.Gonadosomatic index were ranged between 13.69-16.65%, ovosomatic index: 11.26-14.62%. The weight of the egg varied from 1.45-1.69 mg/egg with the diameter in between 1.37-1.66 mm. The number of eggs per kg broodstock varied from 74,473-88,787 with fertilization rate of : 67.15-93.29%, and hatching rate were ranged from 88.33%-96.62%. The feed with 8% lipid level showed the highest value of gonadosomatic index (16.65%), fertilization rate (93.29%), percentage of larva produced (90.14%), egg weight (1.69 mg) and egg diameter (1.66 mm). Average daily growth rate of the 21 days old offsprings was 18.12%-19.25% with survival rate of 60.25%-75.50%. The feed with 8% lipid level tends to give the best effect on the reproduction parameters and the performances of the seed.
PROFITABILITAS DAN KERAGAAN PERTUMBUHAN BENIH IKAN Tor tambroides DENGAN FREKUENSI PAKAN YANG BERBEDA Subagja, Jojo; Radona, Deni
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4612.971 KB) | DOI: 10.14203/beritabiologi.v17i2.3000

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Fish of Tor genus have a slow growth character that resulted in increasing production cost. The high production cost is related to the high price of feed. Fish growth can be triggered by an optimal feeding. A feeding strategy is needed to improve the efficiency of production cost. This study aimed to determine the profitability and the performance of the seedling thai mahseer fish with different feeding frequencies. This study was conducted in Germplasm Research Station, Cijeruk in March-April 2014. The fish used in the experiment have a standard length of 2.29 ± 0.12 cm and weight of 0.32 ± 0.07 g derived from induced breeding spawning of the broodstock natural catches which was adapted for four years in pond concrete. The fish were reared in the aquarium (dimension= 40 x 30 x 30 cm) with a water height of 20 cm and a stocking density of 50 individuals each. The experiment was designed by using three treatments and three replications for each treatment. Fish were feed using commercial feed (protein content = 28%) as much as 20% per day of the total biomass. The treatment observed was feeding frequency, namely (A) three times/day, (B) twice/day, (C) once/day. Results of the experiment for 40 days showed that the increasing values of length, weight, specific growth rate, survival rate, biomass and FCR were not significantly difference (P>0.05). The highest benefit value was obtained in the treatment of feeding with a frequency of three times/day (Rp. 332.304 ± 14.819,-).
Reproductive Characteristics and Induced Breeding in Heterobranchus longifilis Subagja, Jojo; Gustiano, Rudhy
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.180

Abstract

Two experiments were conducted in the IRD-Gamet Laboratory, Montpellier, France. The first was to evaluate the recovery of Heterobranchus longifilis female gonad after induced ovulation, under tropical condition. Oocyte diameter was used as indicator of the recovery rate following by induced breeding, hatching rate and abnormality of larvae. The results showed that the recovery was about 28 to 35 days after ovulation. The second was carried out to improve the milt production by hormone treatments. Volume of intratesticular sperm and the number of spermatozoa were observed. The results indicated that the treatmens did not prove any significant difference.
PENGARUH FREKUENSI PEMBERIAN PAKAN PADA PENDEDERAN KE DUA IKAN LELE DUMBO YANG DIPELIHARA DI KOLAM TANAH Sundari, Sri; Nugroho, Estu; Subagja, Jojo
Jurnal Sains Natural Vol 1, No 1 (2011): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.881 KB) | DOI: 10.31938/jsn.v1i1.5

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Effect of Feed Frequency on Second Rearing Larvae of Catfish Culture in Earthen Pond.           Breeding is an important part in business scale of catfish. The critical time of catfish culture is in second rearing of larvae (from 2-3 cm to 8-10 cm) due to their canibalism. The successibility of seed rearing is depend on continuesly feed reservation, including feed management. The objective of this research was to get information about feed frequency given to seed reared in earthen pond. Research was conducted using farmer?s earthen pond in Pabuaran, Kemang Bogor district. Cat fish seed originated from farmer located in Bogor, with size of 2-3 cm per each and stocking density of 100 fish/m2.  Treatments were feed frequency i.e. 2x; 4x and 6x in a day or night. Observed parameter were survival  and growth rate, including water quality. Block Randomized Design of Experiment were used with two replications. Samping was conducted every 7 days. Result showed that there was statistically significant differences (P<0.05) among treatment in survival rate, while no statistically differences in growth rate of seeds length and weight. Feed frequency 2x at night gived the best result of survival rate and percentage of ?filter selection? was up to 80% and 37.76% respectivelly.Keywords : Feed frequency, second rearing, catfish ABSTRAK          Salah satu skala bisnis ikan lele yang penting adalah pembenihan. Pada pembenihan lele, masa kritis salah satunya ditemui pada tahapan pendederan kedua (dari ukuran 2-3 cm ke 8-10 cm). Keberhasilan pendederan ini  sangat tergantung pada kesediaan pakan yang kontinu, sehingga dapat menekan kanibalisme yang ada. Tujuan penelitian ini yaitu untuk mendapatkan informasi frekuensi pakan yang diberikan dengan tepat pada  pendederan ikan lele yang dipelihara di kolam tanah. Penelitian ini dilaksanakan di kolam tanah di daerah pabuaran, Kemang, Bogor. Ikan uji yang digunakan adalah benih ikan lele ukuran ± 2-3 cm/ekor, dengan kepadatan 100 ekor/m2. Perlakuan yang diamati adalah frekuensi pemberian pakan, yaitu i) 2x, ii) 4x dan iii) 6x, baik dilakukan pada siang maupun malam hari. Secara garis besar  parameter yang diamati dalam penelitian ini meliputi pertumbuhan, dan kelangsungan hidup serta sifat fisika dan kimia air. Rancangan percobaan yang digunakan adalah rancangan acak blok yaitu berupa waktu siang dan malam  dengan 2 ulangan waktu. Sampling dilakukan 7 hari sekali. Hasil yang diperoleh menunjukkan adanya perbedaan yang nyata (P<0,05) dari perlakuan terhadap sintasan benih, namun tidak berbeda nyata pada laju pertumbuhan harian berat dan panjang benih. Pemberian pakan dengan frekuensi 2x pada malam hari memberikan pengaruh yang terbaik dengan sintasan mencapai 80%, dengan persentase lolos saring mencapai 37.76%.Kata kunci : frekuensi pakan, pendederan 2, ikan lele
LOW WATER TEMPERATURE AND ITS EFFECTS ON STRESS RESPONSE OF GREY MULLETS Mugil cephalus ACCLIMATED IN FRESHWATER Prakoso, Vitas Atmadi; Subagja, Jojo; Chang, Young Jin
Indonesian Aquaculture Journal Vol 10, No 1 (2015): (June 2015)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.582 KB) | DOI: 10.15578/iaj.10.1.2015.41-45

Abstract

Grey mullet (Mugil cephalus) can adapt to saline and freshwater. Although belonged to euryhaline species, but information regarding their stress response on low temperature. Environmental disturbance such as low water temperature may effect their physiological condition. These information can be useful for aquaculture development of this species in freshwater. Therefore, the purpose of study was to investigate the effects of low water temperature on the stress response of grey mullets Mugil cephalus acclimated in freshwater. The blood samples of experimental fish (TL: 28.2±1.1 cm, BW: 198.6±25.9 g) were collected during winter season when the water temperature of controlled rearing system was stable at 25°C and uncontrolled rearing system slowly dropped until 12°C. Their stress response on both rearing systems was observed. The results showed that low temperature affected to lower the behavior activity and increase the stress response of grey mullets. The breath frequency of grey mullet regarding their opercular movement at 12°C was 74-97 breath/min., while at 25°C it was 95-114 breath/min. Hematocrit (Ht) and hemoglobin (Hb) were shown higher values of 43.5% and 9.5 g/dL, respectively at 25°C than 12°C (28.0% and 7.1 g/dL, respectively). The tendencies of cortisol and glucose level increased with the lowering temperature, showing higher value of 264.8 ng/mL and 35.5 mg/dL in 12°C than 5.5 ng/mL and 32.7 mg/dL in 25°C. The chemical properties of blood in grey mullets showed same tendency comparing between 12°C and 25°C, there was no significant different between each temperature, except for chloride (P&lt;0.05). Chloride value was higher at 25°C, while other blood components such as osmolality, sodium, potassium, and magnesium were showing no significant differences. However, the results showed lower values at 12°C in every blood components, except for chloride. In conclusion, lowering water temperature to 12°C had impact as stressor to the behavior and stress response of grey mullets acclimated in freshwater.