I Ketut Suatha
Fakultas Kedokteran Hewan,Universtias Udayana

Published : 34 Documents
Articles

Found 34 Documents
Search

DINAMIKA POPULASI MONYET EKOR PANJANG (MACACA FASCICULARIS) DI HUTAN WISATA ALAS KEDATON TABANAN

Buletin Veteriner Udayana Vol. 1 No. 2 Agustus 2009
Publisher : Buletin Veteriner Udayana

Show Abstract | Original Source | Check in Google Scholar

Abstract

Overall population dynamic were observed in identified individuals between August andOctober 2008, in large group of long failed macaques in the AlasKedaton, Bali. Totalpopulation was 364 monkeys consisted of 54 (14,8%) adult males, 104 (28,6%) adultfemales, 164 (45,1%) juvenile and 42 (11,5%) infant. They were divided into 4 differentsmall social groups i.e., Parking area group, North area group, Centre area group and Southarea group. Ratio of adult male and adult female was 1: 2.Population densitiesof Macaca fascicularisin Alas Kedaton were 30 monkeys / Ha andpopulation natalities were 11, 5%.

Keragaman Silak Tanduk Sapi Bali Jantan dan Betina

Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : Buletin Veteriner Udayana

Show Abstract | Original Source | Check in Google Scholar

Abstract

Sapi merupakan salah satu hewan bertanduk dan tanduk berfungsi sebagai pertahanan diridari predator. Pada penelitian ini dicari persentase silak, dari berbagai ragam silak, yakni :silak bajeg, congklok, cono, pendang, manggulgangsa, dan anoa yang ditemukan pada sapibali di Pasar Hewan Beringkit. Penelitian ini menggunakan sampel 1000 pasang tanduksilak sapi bali dewasa, yang terdiri dari 500 pasang tanduk sapi bali jantan dan 500 pasangtanduk sapi bali betina. Hasil penelitian menunjukkan pada sapi bali jantan ditemukantanduk silak bajeg (26,3%) tanduk silak congklok (1,6%), tanduk silak cono (9,1%), tanduksilak pendang (13%), sedangkan tidak ditemukan ragam silak manggulgangsa dan tanduksilak anoa. Sapi bali betina terdiri dari 3,5% silak bajeg, 0,1% silak congklok, 36%, 5,9%silak cono, 2,1% silak pedang , 2,4% silak manggulgangsa dan tanduk silak anoa. Silaktanduk yang paling banyak ditemukan pada sapi bali jantan yaitu tanduk silak bajeg,sedangkan pada sapi bali betina yaitu tanduk silak cono. Silak pada sapi bali bervariasi,baik dari jenis, ukuran dan bentuk. Silak manggulgangsa dan anoa hanya muncul pada sapibali betina.

VASEKTOMI DAN PEMOTONGAN TARING PADA MONYET EKOR PANJANG (Macaca fascicularis) DI KAWASAN WISATA PURA BATU PAGEH, DESA UNGASAN BADUNG

Jurnal Udayana Mengabdi Vol 11, No 2 (2012): Volume 11 No.2 – September 2012
Publisher : Jurnal Udayana Mengabdi

Show Abstract | Original Source | Check in Google Scholar

Abstract

Population growth and aggressiveness are important factors on macaque populations that their habitats are used as tourist destinations. Controlling those two factors may decrease the negative impact of the macaque population both to the environment and the internal circumstance of the population. This Community Service was packed as a vasectomy and canine cutting services on the adult male of long tailed macaques occupying a tourist destination of Pura Batu Pageh, Ungasan Village, Badung Regency. Vasectomy is a surgical division of all or part of vas deferens to induce sterility. Surgical resection of part of vas deferens used a midline slicing method. In this program, one selectively adult male was successfully vasectomized and the four canines were also cut successfully. Later inspection showed that there was no any problem with the individual that formerly subjected to medical treatments and even the wounds went to be healing completely.

CENTELLA ASIATICA EXTRACT INCREASED ON THE LEVEL OF INTERLEUKIN 6 (IL-6) IN MICE

INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 1 Januari 2011
Publisher : Udayana University

Show Abstract | Original Source | Check in Google Scholar

Abstract

Salmonellosis is still problem in many developing countries including Indonesia. Themain problem in controlling and handling the disease is that only few antibiotics are availableto cure the disease. In addition, the prolonged use of such antibiotics often lead to bacterialresistant against the antibiotics. A herbal drugs such as Centella asiatica (in Indonesia isknown as pegagan) contains triterphenoid saphonins which acts as immunostimulant capableof enhancing the phagocytic activity of macrophages. However, no study has been conductedto investigate the use pegagan in activating macrophage of mice infected with Salmonellatyphi. A study was therefore conducted to find out the ability of Cantella asiatica inenhancing on the level interluekine (IL)-6 following challenge with Salmonella typhi. It istherefore expected that herbal drug such as Cantella asiatica can be used as an alternativemedicine to prevent and cure salmonellosis in both animals and human.Experimental laboratory studies were conducted using Completely FactorialRandomized Design. Mice were divided into 4 groups and they were treated respectively withdestilated water (negative control), 125, 250, and 500 mg/kg bw of Centella asiatica extract.The treatment was conducted daily for 2 weeks and the mice were then inoculated with 105cells of S. typhi. The level of IL-6 response were examined 24 hours, 2 weeks, and 4 weeksafter inoculation with S. typhi.The result showed that treatment of mice with Centella asiatica extract significantly(p<0,05) enhaced IL-6 level of Balb/c mice following inoculation with S. typhi. The highestIL-6 level were observed in mice treated Centella asiatica extract at the dose of 500 mg/kgBW (385,9257±125,4314 pg/ml serum). And the highest IL-6 level were observed at 2 weeksfollowing inoculation with S. typhi (533.4262 ± 81.7184 pg / ml).A further study is recomended to examine the celluler immune response and moredetailed study on the humoral immune response of animals or human before this herbal isused as alternatif medicine to prevent and cure typhoid fever. It is also important to study thebest preparation, the half life, and the side effect of Centella asiatica in human and animals.

Vitrifikasi Blastosis Mencit dengan Metode Kriolupv ?O

Jurnal Veteriner Vol 10, No 4 (2009)
Publisher : Jurnal Veteriner

Show Abstract | Original Source | Check in Google Scholar

Abstract

Cryopreservation is an ultra rapid freezing process to preserve tissue or organ. The studywas conducted to identify the effectiveness of cryoloop vitrification method and the viability ofembryos following vitrification. Embryos at blastocyst stage were vitrified by placing them inequilibration medium containing 10% ethylene glycol (EG) and phosphate buffer saline (PBS) wichsupplemented with 20% new born calf serum for 8-10 minutes. The blastocysts were then removedand put in vitrification medium (15% dimethyl sulfoxide, 15% EG, and 0.5M sucrose), and theprocess in the vitrifivcation medium not longer than 25-30 seconds. The blastocysts were immediatelytransferred to the vitrification medium film in the cryoloop and plunged into 100 ml liquid nitrogen.The warming process was done by immersing the cryoloop which carried the vitrified blastocstsinto PBS supplemented with 20% serum and 0.5M sucrose for 1 minute, and then removed to samesolution supplemented with 0.25M sucrose and 0.1M sucrose for 2 minutes respectively. Theblastocysts were washed 4 times in kalium simplex optimized medium (KSOM) and cultured indrops of KSOM in 5% CO2 incubator at 370C. The observations were done every 6 hours for 48hours using inverted microscope ( Olympus IX70 Japan). The viability of embryos was assessed onthe basis of the intact morphology, reexpansion of the blastosul, and the development of embryosinto advance stage. The results showed that 85.71% of vitrified embryos, developed into advancestages and 19% of them hatched. In conclusion the cryoloop can be used to vitrify the embryos.

Pertumbuhan Alometri Dimensi Panjang dan Lingkar Tubuh Sapi Bali Jantan

Jurnal Veteriner Vol 11, No 1 (2010)
Publisher : Jurnal Veteriner

Show Abstract | Original Source | Check in Google Scholar

Abstract

Research was conducted at Bukit Jimbaran, Badung Bali from September to October 2007 to investigatethe lenght and circular body dimension of Bali cattle. Ten young male bali cattle 0.5-1.5 year old weremeasured for three times at 2 weeks interval. Collected data was analysed with regretion-corelation byallometric growth lines Y = aXb. F test and coeficient-correlation was then used to chek the validity andacurate models. Result showed that the lenght head, neck, body anterior and posterior had medium potentialgrowth. Circular neck anterior grew early with a high potential. The circular neck posterior growth potentialwas medium, where as circular thorax and abdomen was law poitential growth.

Kebuntingan Hasil Transfer Blastosis Mencit yang Dibekukan dengan Metode Vitrifikasi Kriolup

Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Jurnal Veteriner

Show Abstract | Original Source | Check in Google Scholar

Abstract

The aim of the study was to assess the viability of vitrified embryo using cryoloop as a carrier ofembryo. The blastocyst stage embryos were collected from superovulated mice. Embryos were frozenusing vitrification method and vitrified embryos were loaded on copper filament cryoloop before dipped inliquid nitrogen. The viability of vitrified embryos was assess in vitro by medium cultered and in vivo bytransfered them to recipient mice. The result shows the viability of vitrified embryos was 85,7% after 24hours cultured and the embryos were born from two pregnant recipient mice out of nine (22%) or fouroffspring out of 63 trasfered embryos (6%). In conclusion, vitrified blatocyst stage embryos using cryoloopas a carrier could keep the viability of the embryos and they could be transfered to the recipient mice andwere born normally.

Perbedaan Aktivitas Ovarium Sapi Bali Kanan dan Kiri serta Morfologi Oosit yang Dikoleksi Menggunakan Metode Slicing

Indonesia Medicus Veterinus Vol.1 (1) 2012
Publisher : Indonesia Medicus Veterinus

Show Abstract | Original Source | Check in Google Scholar

Abstract

Sapi bali (Bos banteng) adalah jenis sapi keturunan banteng dan merupakan plasma nutfah ternak asli yang terdapat di Indonesia. Ovarium, merupakan bagian organ kelamin betina yang utama, bentuk dan ukuran ovarium, berbeda-beda setiap spesies, umur, dan status reproduksinya. Pada sapi ovarium berbentuk oval dan bervariasi dalam ukuran menurut struktur yang berada di dalamnya. Untuk itu diperlukan usaha untuk mengetahui aktivitas ovarium sapi bali kanan dan kiri serta morfologi oosit yang dikoleksi dengan metode slicing.Penelitian dilakukan dengan membandingkan aktivitas ovarium kanan dan kiri dalam hal panjang, lebar, jumlah korpus luteum, ukuran korpus luteum, jumlah folikel, dan jumlah oosit dari ovarium tersebut serta morfologi oosit sapi bali yang dikoleksi menggunakan metode slicing. Sampel yang digunakan berjumlah 18 pasang ovarium kanan dan kiri dari sapi bali yang telah dipotong di RPH Pesanggaran. Data yang diperoleh dianalisis dengan uji chi-square dan dilanjutkan dengan uji wilcoxon.Hasil penelitian menunjukkan adanya perbedaan aktivitas antara ovarium kanan dan ovarium kiri, tetapi pengujian secara statistika dengan uji wilcoxon tidak menunjukkan perbedaan yang signifikan (p>0,05). Pada hasil koleksi diperoleh oosit dengan morfologi yang memenuhi seluruh kriteria (A, B, C,dan D).Dari penelitian ini dapat disimpulkan bahwa nampak terjadi perbedaan aktivitas antara ovarium kanan dengan ovarium kiri, namun secara statistik tidak memberikan perbedaan yang nyata. Dengan metode slicing diperoleh oosit dengan morfologi yang memenuhi kriteria morfologi A sampai D.

Gambaran Klinik Sapi Bali Tertular Rabies di Ungasan, Kutuh dan Peminge

Indonesia Medicus Veterinus Vol 1 (3) 2012
Publisher : Indonesia Medicus Veterinus

Show Abstract | Original Source | Check in Google Scholar

Abstract

Rabies di Bali terjadi sejak Oktober 2008. Awalnya Bali merupakan provinsi bebas rabies. Rabies di Bali telah menyebar ke beberapa kabupaten seperti Denpasar, Badung, Buleleng, Bangli, Tabanan, Gianyar, dan Karangasem. Hingga saat ini korban rabies pada manusia mencapai 122 orang. Desa Ungasan, Kuta Selatan, Badung, merupakan tempat awal terjadinya rabies pada manusia, dengan adanya laporan 4 warga meninggal, 2 diantaranya positif rabies. Rabies pada sapi bali dilaporkan telah terjadi di Kabupaten Tabanan. Berdasarkan informasi dari masyarakat desa Kutuh (tetangga desa Ungasan) tentang sapi-sapi milik warga yang mati dengan tanda-tanda rabies bersamaan dengan kejadian rabies pada anjing dan manusia.

Struktur Genetika Populasi Monyet Ekor Panjang Di Alas Kedaton Menggunakan Marka Molekul Mikrosatelit D18S536

Indonesia Medicus Veterinus Vol 2 (1) 2013
Publisher : Indonesia Medicus Veterinus

Show Abstract | Original Source | Check in Google Scholar

Abstract

Struktur genetika populasi adalah kondisi intrinsik (genetik) suatu populasi. Pengungkapan struktur genetika dapat memberikan gambaran apakah kehidupan suatu populasi dalam keadaan aman atau terancam. Populasi dengan struktur genetika yang tinggi akan membuat potensial evolusi yang baik terhadap faktor-faktor yang bersifat stokastik. Pada tingkat DNA dengan menggunakan marka molekul mikrosatelit struktur senetika suatu populasi dapat diungkap. Marka molekul mikrosatelit merupakan segmen langsung dari genom (DNA) sehingga variasi genetik yang ditemukan mencerminkan variasi genetik yang sebenarnya. Penelitian menggunakan lokus mikrosatelit D18S536 untuk mengkaji struktur genetika populasi monyet ekor panjang di Alas Kedaton yang meliputi jumlah dan jenis alel, frekuensi alel, dan heterosigositas. Sejumlah 16 sampel darah dari populasi monyet ekor panjang di Alas Kedaton sebagai sumber DNA. DNA diekstraksi dengan menggunakan QIAamp DNA Blood Mini Kit dari Qiagen. Lokus mikrosatelit D18S536 kemudian di PCR, sebanyak 30 siklus dengan suhu annealing 450 C. Selanjutnya, pada gel poliakrilamid 7% alel dipisahkan dengan elekrtoforesis dan dimunculkan dengan pewarnaan perak. Hasil penelitian mengidentifikasi 5 jenis alel pada lokus D18S536 dalam populasi monyet ekor panjang di Alas Kedaton dengan panjang alel berkisar antara 160-176 pasang basa. Frekuensi alel bervariasi, alel 160 (0,31) memiliki frekuensi tertinggi di susul alel 164 (0,22), alel 168 (0,22), alel 172 (0,19) dan alel 176 (0,06). Heterosigositas populasi monyet ekor panjang di Alas Kedaton menggunakan lokus D18S536 sebesar 0,79. Dari hasil penelitian dapat disimpulkan bahwa lokus D18S536 pada populasi monyet ekor panjang di Alas Kedaton bersifat polimorfik.