Rina Sriwati
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Deteksi dan Identifikasi Cendawan Endofit Trichoderma yang Berasosiasi pada Tanaman Kakao Sriwati, Rina; Chamzurni, Tjut; Sukarman, Sukarman
Jurnal Agrista Vol 15, No 1 (2011): Volume 15 Nomor 1 April 2011
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Detection and Identification of Endophyte Trichoderma Fungi Associated Cocoa PlantABSTRACT. The endophytic fungi are non pathogenic fungi and almost all of them associated with plant cells without any symptoms. Endophites fungi that associated with cacao plant from East Aceh has been isolated. Based on morphological and molecular identification was found two species of fungi Trichoderma spp which is fungal antagonist. Molecular identification have provided the species of Trichoderma virens and Trichoderma longibrachiatum. Trichoderma virens had goog poteny as biological control agains patogen.
Trichoderma virens ISOLATED FROM COCOA PLANTATION IN ACEH AS BIODECOMPOSER COCOA POD HUSK Sriwati, Rina; Chamzurni, Tjut; Bukhari, Bukhari; Sanjani, Anwar
Jurnal Natural Volume 13, Number 1, March 2013
Publisher : Syiah Kuala University

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Sustainable development on cocoa farming have been places great emphasis on the management of natural resources for Cocoa pod husk. Until now, there has been much public attention for a farming waste, environment polluting and a place to develop various diseases, especially fungal Phytopthora. To overcome these problems, it needs to utilize alternative cocoa pods. The aims of this study were: (1) to determine the potential use of Trichoderma virens isolated from cocoa plantation in Aceh as bio-decomposer in the composting process cocoa pods. The composition of potential use experiment; Manure+Trichoderma (PT); Cocoa pod husk+Trichoderma (KT); Cocoa pod husk + Manure (KP); Cocoa pod husk +Manure+Trichoderma (KPT) has been tested. The analysis result showed that the combined use of Trichoderma virens compost although no effect on the color, texture and smell of compost but could be effect on characterized of compost maturity by characteristics of C/N compost. The content of C/N compost on all treatments in accordance with the standard compost maturity thus considered quite feasible to be applied to the crop.
Nematode Species Inhabiting Pine Trees Killed by the Pinewood Nematode, Bursaphelenchus xylophilus Sriwati, Rina
Jurnal Agrista 2008: Edisi Khusus Nomor 1 November 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Spesies Nematoda Habitat Pada Pohon Pinus Mati Akibat Terserang Penyakit Layu Nematoda (Bursaphelenchus xylophilus).ABSTRAK. Spesies-spesies nematode habitat pada pohon pinus mati akibat terserang nematode (Bursaphelenchus xylophilus) telah diisolasi dan diidentifikasi. Lima belas spesies nematode telah terdeteksi, diantaranya (i) lima spesies nematode mycophagus: B. xylophilus, Bursaphilenchus n. sp., dan B. sinensis, Tylencida sp. 1, Tylencida sp. 2; (ii) Sembilan spesies nematode saprophagus: Diplogasterida sp. 1, Diplogasterida sp. 2, Diplogasterida sp. 3, Diplogasterida sp. 4, Monhisterida sp. 1, Monhisterida sp.2, Rhabditida sp. 1, Rhabditida sp. 2, Plectidae sp.; dan (iii) satu spesies nematode predator yaitu Mononchida sp. Dari setiap waktu pengamatan nematode B. xylophilus ditemukan merupakan spesies nematode yang paling dominan, dan diikuti oleh spesies Diplogasterida sp. 1.
KEMAMPUAN BERTAHAN HIDUP TRICHODERMA HARZIANUM DAN TRICHODERMA VIRENS SETELAH DITUMBUHKAN BERSAMA DENGAN JAMUR PATOGEN TULAR TANAH SECARA IN VITRO Sriwati, Rina; Chamzurni, T.; Kemalasari, L.
Jurnal Floratek Vol 9, No 1 (2014): April 2014
Publisher : Universitas Syiah Kuala

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The experiment was conducted at the Laboratory of Plant Pathology Faculty of Agriculture, Syiah Kuala University from February to June 2013. The purpose of study was to evaluate the survival of T. harzianum and T. virens after cohabiting with soil borne pathogenic fungi (Fusarium sp, Rhigidoporus sp, and Sclerotium sp) as well as the type of interaction. Trichoderma fungi and fungal pathogens were grown on PDA in vitro, and then observed their growth after being reisolated on 3 different growth zones. The results showed that T. harzianum and T. virens  growing with Fusarium sp were capable to grow over the space. It was evident from the results of reinoculation on a petridish B (border zone) and C (zone Trichoderma) colonies, that T. harzianum and T. virens regrew, while the pathogens were not able to regrow. When cohabiting with Sclerotium sp and Rhigidoporus sp, antagonistic fungus regrew after reisolation but pathogens also grew on Petridis B (border zone). It indicates that on the contact area (petri dish B), Sclerotium sp and Rhigidoporus sp conduct defense over the competition. Regrowth of fungal pathogens on contact area (border) indicates that the fungus has a high level of competition. Antagonist agents such as T. virens and T. harzianum were able to survive and regrow after cohabiting with fungal pathogens and showed type A interaction (against Fusarium sp), while cohabiting with Sclerotium sp and Rhigidoporus sp showed the type B interaction.
FORMULATION OF TRICHODERMA VIRENS ORIGIN OF ACEH COCOA CONTROLLING BLACK POD DISEASE CAUSED BY PHYTOPHTORA PALMIVORA CHAMZURNI, TJUT; SRIWATI, RINA; MUARIF, RIZKY; AMIN, BUNI; ULIM, ABDUH
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Trichoderma virens have been isolated from rooting cocoa in Aceh and in the laboratory have been reported capable of inhibiting the growth of P. palmivora in vitro. However, the formulations of T. virens from Aceh can be used as an alternative product for farmers in controlling black pod disease of cocoa. This research was conducted t the Laboratory of Plant Pathology College of Agriculture Syiah Kuala University and cocoa fruit samples obtained from cocoa plantations owned by farmers in Pidie Jaya District of Aceh. The purpose of the study was to obtain an application formulations strains of T. virensfrom Aceh, as one of the biological control study consisted of seven treatments, namely W (water), Pest (Mancozeb Fungicide), Det (Detergent), Det+Tv( detergent+strainsof T.virens), P+Tv (Adhesive +strainsof T.virens), Co+Tv( Corn Oil+strainsof T.virens), Po+Tv (Palm oil+strainsof T.virens) and each treatment was repeated 10 times to obtain70 experimental units. The parameters observed incubation period, disease incidence (%), and the diameter of the spots (cm). Application results show that the strains of T. virens Origin Aceh effective in controlling P. palmivora on cocoa pods and the best  formulation in controlling P. palmivora on cocoa pods are corn oil+T. virens formulation (Co +Tv) compared with other formulations are characterized by the length of the incubation period, the low incidence of P. palmivora disease, and the small diameter of the spots on the cocoa pods.
Fig Pollinating Wasp Transfers Nematodes into Figs of Ficus racemosa in Sumatra, Indonesia Jauharlina, Jauharlina; Sriwati, Rina; Yusmaini, .; Kanzaki, Natsumi; Compton, Stephen
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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The fruits (figs) of fig trees (Ficus spp, known as ‘bak ara’ in Aceh), are the source of food for many species of faunas in the forest, including birds, monkeys, orangutans, etc.  Pollination within the figs totally depends on female fig wasps that belong to family Agaonidae. Fig trees and their pollinating wasps rely on each other to survive.  Female fig wasps are known to transport nematodes into receptive figs when the wasps enter the figs to lay  eggs.  An investigation on the nematodes carried by female pollinating wasps Ceratosolen fusciceps Mayr into figs of Ficus racemosa was conducted in Sumatra, Indonesia. The figs on the trees were regularly sampled to determine the presence of nematodes and infer their ecology. The Baermann funnel method was employed to extract the nematodes from the figs.  Eight species of nematodes were recorded from the figs, two of which are still unidentified.  The species found were (1) Teratodiplogaster fignewmani, (2) Teratodiplogaster sp., (3) Parasitodiplogaster sp., (4) Schistonchus sp1., (5) Schistonchus sp2., (6) Mononchoides sp., (7) and (8) two undescribed Diplogastridae species (‘umbrella-like’ species 1 and species 2).  This is the most diverse fig nematode community recorded. The highest nematode populations were routinely found in D-phase figs, when the new generations of wasps were about to emerge.   Details of the ecology of each nematode species are likely to differ, but as a group they did not seem to significantly affect seed and wasp development in F. racemosa figs
EFEKTIVITAS DOSIS DAN WAKTU APLIKASI Trichoderma virens TERHADAP SERANGAN Sclerotium rolfsii PADA KEDELAI Chamzurni, Tjut; Sriwati, Rina; Rahel, Rahel
Jurnal Floratek Vol 6, No 1 (2011)
Publisher : Universitas Syiah Kuala

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            This study was aimed at obtaining an effective dose and application timing of Trichoderma in controlling wilt disease caused by Sclerotium on soybean. This study used a factorial complete ramdomized design with 8 combination of treatments and 4 replications. There were two factors studied, dose and timing of Trichoderma. Four level doses factor were studied, i.e. 75, 150, 225, and 300 g.polybag-1, while timing of application consisted of 2 levels, 7 days before planting and at the planting time. The observed variables were germination rate, incubation period, length of lesion formed on the base of the stem, and dry weight of seeds per plant. Data of variables observed was analized by analysis of variance and followed by least significance different test at level 5%.  The results showed that dose of Trichoderma gave a significant effect on seed germination rate, incubation period, length of lesion and dry weight of seed per plant. Dose of Trichoderma 300 g.polibag-1 was the best and gave  germination rate of seed up to 84,38%, incubation period 8 days, length of lesion 1,35 cm and dry weight of seed 24,13 g. The timing of application gave a significant effect only on dry weight of seed per plant.  The best timing of application was found at 7days before planting and no interaction between doses and time of application of Trichoderma.
CHARACTERISTIC SYMPTOMS OF Phytophthora palmivora ON COCOA LEAVES Sriwati, Rina; Muarif, Rizky
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Syiah Kuala University

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The research was aimed to determine the characteristics symptoms of Phytophthora palmivora on cocoa leaves, and was conducted in Plant Diseases Laboratory, the Faculty of Agriculture Syiah Kuala University. Mature cacao leaves with almost the same size in the quotation were used at Lab. Plant disease. Suspensions of P. palmivora were inoculated on leaves by spraying techniques with several concentrations of treatment, 1x102, 1x104, 1x106. The results showed that cocoa leaves’ symptoms were getting yellow colour around the veins and the whole leaves eventually showed lesion on the third day after spraying. The higher spores’ population of P. palmivora could impact higher invasive infections. Characteristic symptoms will be very helpful in the process of observation of early disease in the nursery.
PENGARUH TRICHODERMA TERHADAP PERKECAMBAHAN DAN PERTUMBUHAN BIBIT KAKAO, TOMAT, DAN KEDELAI Nurahmi, Erida; Susanna, Susanna; Sriwati, Rina
Jurnal Floratek Vol 7, No 1 (2012)
Publisher : Universitas Syiah Kuala

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Trichoderma is a free-living fungus, commonly can be found in soil and root ecosystem.  Extensively, it is capable of producing antibiotics, parasite to other fungus, and microorganism that cause diseases on plants.  The objectives of the experiment were to study effects of Trichoderma on germination and growth of cacao, tomato, and soybean. The result showed that provision of Trichoderma (T. harzianum and T. virens) conidia suspension using seed submersion technique did not affect seed germination of cacao, tomato, and soybean, but significantly affected cacao root extension. Provision of Trichoderma through seed submersion on sand box germination gave a positive response to tomato plant, tolerance to cacao plant, and a negative response to soybean plant.  The causing factors of difference responses varied including concentration, application techniques, and kinds of seed.
PENGARUH KOMBINASI SPESIES TRICHODERMA DAN FREKUENSI PENYEMPROTAN TERHADAP INTENSITAS PENYAKIT BUSUK BUAH (PHYTOPHTHORA PALMIVORA) DAN HASIL PANEN KAKAO Ferayanti, Fenty; Sriwati, Rina; harnelly, Essy
Jurnal Floratek Vol 11, No 2 (2016): Oktober 2016
Publisher : Universitas Syiah Kuala

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The research objective was to determine the effect of Trichoderma species and spraying frequency on the intensity of  black pod disease (Phytophthora palmivora) and cocoa yield. This study was conducted in farmers field in The East Aceh District, at June to Desember 2014. The choice of location was based on a history of the cocoa pod disease in the previous period or the location was endemic. The research was arranged in randomized block design non factorial, consisted of 10 treatments and 4 replications, i.e : A = control, B (T. harzianum + spraying frequency of 1 times), C (T. harzianum + spraying frequency of  2 times), D (T. harzianum + spraying frequency of 3 times), E (T. viren + spraying frequency of 1 times), F (T. viren + spraying frequency of 2 times), G (T. viren + spraying frequency of 3 times), H (T. asperellum + spraying frequency of 1 times), I (T. asperellum + spraying frequency of 2 times), J (T. asperellum + spraying frequency of 3 times). Each treatment consisted of 5 plants, the number of plant are 200 plants. At each plant is set 10 cacao pod as samples for observation of disease intensity. The results of the research showed that the the lowest intensity of black pod disease due to the combination treatment of Trichoderma species and spraying on 2 and 3 months after application frequency found in treatment D (T. harzianum + 3 times spraying  frequency) was 12.50 % and 13.13 %. The highest cocoa fruit yield found in treatment D (T. harzianum + 3 times spraying frequency) was 45.75 kg and the lowest found in treatment A (without Trichoderma / control) was 27.02 kg.