Articles

Physiological and Biochemical Responses to Aluminum Stress in the Root of a Biodiesel Plant Jatropha curcas L. TISTAMA, RADITE; WIDYASTUTI, UTUT; SOPANDIE, DIDY; YOKOTA, AKIHO; AKASHI, KINYA; SUHARSONO, .
HAYATI Journal of Biosciences Vol 19, No 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

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Abstract

We investigated J. curcas responses to aluminum stress, histochemically and biochemically. Histochemical stainings were observed to analysis aluminum accumulation, lipid peroxidation and the loss of plasma membrane integrity on the surface and tissue of the root apex. Enzymatic analysis was conducted to measure malate content in leaf, root and malate efflux in the medium. We used M. malabathricum as a comparison for Al-tolerance plant. J. curcas root elongation was inhibited by 0.4 mM AlCl3, while M. malabathricum root elongation was inhibited by 0.8 mM AlCl3 treatment. Inhibition of root elongation has high correlation with Al accumulation in the root apex, which caused lipid degradation and cell death. Generally, malate content in J. curcas leaf and root was higher than that in M. malabathricum. In the contrary malate efflux from the root into the medium was lower. J. curcas root has a different pattern compared to M. malabathricum in malate synthesis and malate secretion when treated with a different Al concentration. We categorized J. curcas acc IP3 as more sensitive to aluminum than M. malabathricum.
Evaluasi Beberapa Galur-Pup1Tanaman Padi (Oryza sativaL.) pada Larutan Hara dan Lapangan Prasetiyono, Joko; Suhartini, Tintin; Soemantri, Ida Hanarida; Tasliah, ,; Moeljopawiro, Sugiono; Aswidinnoor, Hajrial; Sopandie, Didy; Bustamam, Masdiar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

Phosphorus (P) deficiency is one of major problems in rice cultivation. Development of a tolerant variety to P deficient soil is expected to reduce the needs of P fertilizer. The aim of this research was to evaluate the agronomic performance of Pup1-introgression rice lines. This research consisted of two separate experiments, an evaluation on rice grown in nutrient solution in the greenhouse of ICABIOGRAD, Bogor; and field evaluation at upland rice screening site Taman Bogo, Lampung. The rice lines used were BC2F3 derived from crossing of Situ Bagendit with Kasalath and NIL-C443, and from crossing of Batur with Kasalath and NIL-C443. Molecular analysis was conducted to verifiy the Pup1 introgression in the lines used and successfully confirmed the occurance of homozygote Pup1 segment in those lines. Evaluation of rice lines grown in nutrient solution was performed in a split plot factorial design, using the dose of Al (0 and 45 ppm Al3+) as main plots and dose of P (0.5 and 10 ppm P) as subplots. Field evaluation was performed in split plot design, with P fertilization (0 and 500 kg SP-18 ha-1) as main plots and BC2F3 lines as the subplots. Evaluation using Yoshida nutrient solution showed that the BC2F3 lines had greater total dry weight under low-P condition (37.5-112.5%), especially under Al-toxicity, compared to the respective parental varieties (Situ Bagendit and Batur). Field evaluation showed that the Pup1-introgression lines had greater shoot dry weight than the respective parental lines (10.5-74.82%). However there was no significnant effect of Pup1 introgression in terms of weight of filled grain. Keywords: Al toxicity, BC2F3-Pup1 lines, P-deficiency, rice, Yoshida nutrient solution
SALT TOLERANCE OF TURFGRASS Puccinellia dirtans: II. IONIC INTERACTION IN THE ROOTS Sopandie, Didy; Moritsugu, Masumi; Kawasaki, T.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 23, No 3 (1995): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

In this study, multi-compartment transport boxes were used to determine the mobility of ions in the execised roots. For measuring Na uptake, the concentrations of 1, 50 and 150 mM NaCI were used in the external medium, whilst Ca were 0 and 2. 0 mM. The uptake of K was measured from 1.0 mM KCI in the presence of Na (0.50 and 150 mM) and Ca (0.50 and 2.0 mM). In the present study, 22 Na and 86 Rb were used to label Na and K in the external medium, respectively. Addition of Ca inhibited Na uptake in the roots exposed to 1.0 mM and 50 mM NaCI, thought Ca was relatively less effective in 150 mM. High concentrations of NaCI brought about the reduction of K uptake. Calcium, however, did not show any importance in recovering the uptake of K from the adverse effect of Na when the roots were exposed 10 50 mM NaCI, thought stimulating effect of Ca was observed at 150 mM NaCI. The results revealed that the pattern of ionic interactions in Puccinellia distans differs much with that generally found in most of halophyte plants.
Analisis Marka RAPD yang Te rpaut dengan To leransi terhadap Naungan pada Kedelai Wirnas, Desta; Sopandie, Didy; Trikoesoemaningtyas, ,; Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of the research was to identify RAPD markers linked to QTL related to agronomic traits of soybean under low-light intensity condition.  The genetic material used in the QTL analysis based on RAPD Markers were  Ceneng, Godek, and  F6 RILs derived from hybridization between  Ceneng (tolerant parent) and Godek (sensitve parent). The results of molecular analysis showed that 9 primers were polymorphic and linked to the tolerant parent.  Primers produce 14 RAPD markers which were polymorphic and linked to the tolerant parent. The markers were distributed into a linkage group that containing seven markers. RAPD Markers (OPE15-800, OPM20-800) were linked to two QTL controlling number of productive node and seed weight, respectively.  The marker linked to the tolerant parent could be used as a marker assisted selection for high-yielding soybean lines under low-light intensity.    
Keragaman Karakter Komponen Hasil dan Hasil pada Genotipe Kedelai Hitam Wirnas, Desta; Trikoesoemaningtyas, ,; Sutjahjo, Surjono Hadi; Sopandie, Didy; Rohaeni, Wage Ratna; Marwiyah, Siti; Sumiati, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 3 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

The objective of this research was to determine the trait variance in black soybean lines in terms of yield and yield components. The materials used were F8 lines generated from hybridization of two local varieties, i.e. black seeded Ceneng and greenish yellow seeded Godek, and then selected under single seed descent method, except SC-39-1, SC-62-2 and GC-74-7 that were generated through bulk selection method. The results showed that the traits between plant genotypes were significantly different, except number of productive node and number of unfilled pod. Total variance was contributed more by number of filled pod, seed weight per plant and 100 seed weight. Seed weight per plant and 100 seed weight had high heritability with genetic variance and broad-sense heritability which were significantly different from zero. These characters are important for further selection to improve yields of black soybean lines.Keywords: black soybean lines, broad sense heritability, total variance
Application of Filter Cake on Growth of Upland Sugarcanes Purwono, ,; Sopandie, Didy; Harjadi, Sri Setyati; Mulyanto, Budi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Recently planting site of sugarcane was shifted from lowland to upland area. Sugar cane cultivation in upland has many constraints, especially limited water supply and low nutrition availability. The objectives of this research were to study the influence of  application of composted filter cake on growth and water use efficiency of upland sugar cane. The research was conducted in Jengkol, Kediri. Treatments consist of three factors: frequency of irrigation (once every 1 week, once every 2 weeks, and once every 3 weeks); sugarcane varieties (PS-862 and  PS-864); and  compost doses (0, 2.5, 5, and 7.5 ton ha-1). Split plot design with three replications was used in each  irrigation treatment, using composted filter cake as main plots and sugarcane varieties as sub plots. The results showed that the highest sugar content was  reached at application of 5 ton ha-1   compost  and the  greatest crystal sugar was reached at 3.09 ton ha-1 compost. Compost application at 5 ton ha-1 on each planted row can reduce  frequency of irrigation from once a week to once every 2 weeks.   Keywords: compost of filter cake, frequency of irrigation, upland sugar cane
Karakter Morfo-Fisiologi Daun, Penciri Adaptasi Kedelai terhadap Intensitas Cahaya Rendah Kisman, ,; Khumaida, Nurul; Trikoesoemaningtyas, ,; Sobir, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The main objective of this study was to identify the leaf morpho-physiological characters as markers for adaptation of soybean to low light intensity. The field experiment was conducted using split plot design with 3 replications. First factor was soybean genotype consisted of shade tolerant genotypes (Ceneng, Pangrango) and sensitive genotypes (Godek, Slamet). Second factor was low light intensity consisted of: L0 = control (under full sun light), L1 = 5 days under 50% shading (exposed after 21 days after planting/DAP), L2 = 5 days under dark condition (exposed after 21 DAP), L3 = 3 days 50% shading + 5 days sun light (exposed after 18 DAP), dan L4 = 3 days 50% shading + 3 days sun light + 5 day dark conditions (exposed after 15 DAP). The 50% shading was made artificially using black plastic paranet with 50% light transmitted. Results of this study showed that under the conditions of low light intensity, leaf morpho-physiological characters of soybean that could be used as markers for adaptation were leaf area, specific leaf weight, and chlorophyll content. Leaves of shade tolerant genotypes were wider and thinner and also contained higher chlorophyll especially for chlorophyll b than that of shade sensitive genotypes under low light intensity.   Key words :  Low light intensity, tolerant genotype, leaf area, chlorophyll, specific leaf weight
Kandungan, Resorpsi N dan P serta Specifi c Leaf Area Daun Kakao (Theobroma cacao L.) pada Cekaman Kekeringan Prihastanti, Erma; Tjitrosemito, Soekisman; Sopandie, Didy; Qayim, Ibnul; Leuschner, Christoph
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 1 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Plant nutrient absorptions depend on their environment and plants’ stage of development. Beside from soil, nutrient absorption also proceed through resorption in leaves. The resorption efficiency varies based on types of habitat. Drought stress also influences the efficiency of nutrient resorption, growth rate, and plant development. The purpose of this research was to study the change of nitrogen (N) and phosphorus (P) content and leaf resorption value of N and P, and specifi  c leaf area of 6-years old cacao  grown under drought stress. Research was carried out in a cacao plantation with 7-years old Gliricidia sepium Jacq. as shading trees. The results showed that drought stress with Troughfall Displacement Experiment (TDE) system did not affect the content and resorption level of N and P of cacao leaves. However, N resorption of cacao leaves tended to increase while P resorption decreased. Cacao plants experienced drought stress have the ability to resorp leaf N for 46.64-50.63%, leaf P  for 47.98-58.40%, whereas, the control plants had the leaf N resorption of 45.05-52.97%, and leaf P resorption of 36.64-44.10%. Drought stress for 13 months on 6-years old cacao did not affect  specific  c leaf area.   Keywords: drought stress, N and P resorption, specifi  c leaf area, Theobroma cacao L.
Transformasi Padi Indica Kultivar Batutegi dan Kasalath dengan Gen Regulator HD-Zip untuk Perakitan Varietas Toleran Kekeringan Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; Ouwerkerk, Pieter B.F.; Loedin, Inez Hortense Slamet
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

water deficiency. Genetic engineering at the level of transcription factors (TF) is particulary a promising strategy in developing drought tolerant rice cultivar. HD-Zip genes are TF that function in plant adaptation to some environmental stresses including water deficit. The recombinant plasmid pC1301H Oshox6 which contained HD-Zip Oshox6 gene was placed under a drought inducible promoter called LEA promoter, gusA and hpt genes were driven with CaMV promoter. The aim of research was to obtain indica rice transgenic plants of Batutegi and Kasalath cultivars using pC1301H Oshox6 plasmid. Recombinant plasmid was transformed into immature rice embryos using Agrobacterium tumefaciens. Kasalath cultivar showed a better capacity to form embryogenic calli compared to Batutegi. Transformation efficiency of Batutegi is lower (1.5 - 0.3%) than Kasalath (2.2-28.3%). Regeneration efficiency is 25-83.3% and 7.7-100% for Batutegi and Kasalath, respectively. Number of putative transformant plantlets of Batutegi and Kasalath are 63 and 48 plantlets, respectively. Southern blot analysis (using hpt probe) on 12 independent lines of each Batutegi and Kasalath cultivars showed different gene copy number, ranging from one to four copies of gene.   Keywords: agrobacterium tumefaciens, LEA promoter, HD-Zip Oshox6, rice
Isolasi dan Pengklonan Fragmen cDNA Gen Penyandi H+-ATPase Membran Plasma dari Melastoma malabathricum L. Muzuni, ,; Sopandie, Didy; Suharsono, Utut Widyastuti; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Melastoma malabathricum L. grows well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying acid and aluminum stress is a plasma membrane H+  -ATPase protein encoded by PMA gene. The objective of this research was to isolate and clone the cDNA fragment of MmPMA encoding plasma membrane H+ -ATPase from M. malabathricum L. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of MmPMA  cDNA  had been successfully isolated by PCR by using total cDNA  as  template and PMA primer designed from conserved region for corresponding gene. This fragment had been successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5". Nucleotide sequence analysis showed that the length of MmPMA fragment is 806 bp encoding 268 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MmPMA fragment was 81% identical to part of PMA of Sesbania rostrata, Juglans regia, and Prunus persica. Based on deduced amino acid sequence, MmPMA was 94% identical to part of PMA of Juglans regia; 93% to PMA of S. rostrata, and Arabidopsis thaliana. MmPMA fragment has phosphorylation intermediate domain (DKTGT) and ATP binding domain (KGAP, DPPR, MITGD, and GDGVN).   Keywords: isolation, Melastoma malabathricum L., MmPMA fragment, sequencing
Co-Authors , Hamim , Hamin , Hapsoh , Kisman , Muzuni , Purwono , Sobir , Suharsono , Sumiati , Supijatno , Suwarto , Syafruddin , Tasliah , Trikoesoemaningtyas ,, Trikoesoemaningtyas . SUHARSONO . Suwarno . TRIKOESOEMANINGTYAS ABDUL KARIM MAKARIM ABDUL MUNIF AGUS PURWITO AHMAD JUNAEDI AKIHO YOKOTA Aksarah Pas, Aris Amris Makmur Amrullah Amrullah Anas D Susila Angelita Puji Lestari Asep Setiawan ASMARLAILI SAHAR Atang Sutandi Budi Mulyanto CHAIRANI HANUM Christoph Leuschner Darda Efendi Darman M. Arsyad Desta Wirnas Dwi Andreas Santosa Enny Sudarmonowati Enung Sri Mulyaningsih Erma Prihastanti GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor, Hajrial HAMIM HAMIM Hanedi Darmasetiawan Hasibuan, Halimatus Syahdia IBNUL QAYIM Ida Hanarida Soemantri Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI Kisman Kisman Komaruddin Idris La Muhuria laela Sari, laela Luluk Setyaningsih Masdiar Bustamam Masumi Moritsugu Maya Melati MUHAMMAD AHMAD CHOZIN Muhammad Arif Yudiarto Muhammad Jusuf MUNIF GHULAMAHDI Mustamu, Yohanis Amos Muzuni . Nani Heryani Nurliani Bermawie Nurul Khumaida Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pas, Aris Aksarah Pieter B.F. Ouwerkerk RADITE TISTAMA RAGAPADMI PURNAMANINGSIH ROEDHY POERWANTO SANDRA ARIFIN AZIZ Santosa, Dwi Andreas Santosa Sarsidi Sastrosumarjo Satya Nugroho Setyaningsih, Luluk Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih Sri Rahayu Sri Setyati Harjadi Sri Wilarso Budi SUDIRMAN YAHYA Sugianta, Sugianta SUGIONO MOELJOPAWIRO Suharsono . Suharsono Suharsono Suharsono, S. Sumiati, dan Sungkono Sungkono SURJONO HADI SUTJAHJO Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki TRI KOESOEMANINGTYAS Trikoesoemaningtyas Trikoesoemaningtyas UTUT WIDYASTUTI Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah Wirnas, dan Desta Yadi Setiadi Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati