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The Influence of Low HLA-G Protein Expression on Hsp-70 and VCAM-1 Profile in Preeclampsia Sulistyowati, Sri; Abadi, Agus; Hood, Julianto; Soetjipto, Soetjipto
Indonesian Journal of Obstetrics and Gynecology Vol. 34. No. 4. October 2010
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

Objective: To analyze HLA-G, Hsp-70, and VCAM-1 proteins expression on trophoblast in women with preeclampsia and women with normal pregnancy, and to assess causality relationship between low HLA-G expression and the increased Hsp-70 and VCAM-1 expression in preeclampsia.Method: Observational analytic study with cross sectional design.Results: Trophoblastic HLA-G expression in pregnant women with preeclampsia is lower (p = 0.01) than its expression in women with normal pregnancy. The expressions of Hsp-70 (p = 0.02) and VCAM-1 (p = 0.00) in preeclampsia are higher, compared to normal pregnancy. Regression analysis showed that low HLA-G is a predictor for the increase of trophoblastic Hsp-70 and VCAM-1.Conclusion: Low trophoblastic HLA-G in is a predictor for endothelial dysfunction in women with preeclampsia.[Indones J Obstet Gynecol 2010; 34-4: 185-90] Keywords: preeclampsia, HLA-G, Hsp-70, VCAM-1, endothelial dysfunctionTujuan: Penelitian ini bertujuan untuk menganalisis ekspresi protein HLA-G, Hsp-70 dan VCAM-1 pada trofoblas perempuan preeklampsia dan perempuan hamil normal. Menganalisis adakah hubungan kausatif adanya penurunan HLA-G terhadap peningkatan ekspresi Hsp-70 dan VCAM-1 pada preeklampsia. Metode: Jenis penelitian observasional analitik dengan rancang bangun potong lintang. Hasil: Pada trofoblas ibu hamil preeklampsia ekspresi HLA-G lebih rendah (p = 0,01) dibandingkan ekspresinya pada perempuan hamil normal. Ekspresi Hsp-70 (p = 0,02) dan VCAM-1 (p = 0,00) pada preeklampsia lebih tinggi dibandingkan dengan perempuan hamil normal. Dengan analisis regresi terbukti bahwa HLA-G yang rendah merupakan prediktor untuk terjadinya peningkatan Hsp-70 dan VCAM-1 pada trofoblas.Kesimpulan: HLA-G yang rendah pada trofoblas merupakan prediktor untuk terjadinya kerusakan endotel pada perempuan preeklampsia.[Maj Obstet Ginekol Indones 2010; 34-4: 185-90]Kata kunci: preeklampsia, HLA-G, Hsp-70, VCAM-1, disfungsi endotel
HIV Genotype Analysis from HIV Infected Patients in East Java Area Ismail, Yulia Sari; Soetjipto, Soetjipto; Wasito, Eddy Bagus; Nasronudin, Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Syiah Kuala University

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
The Influence of MMP-3 towards MMP-9 among Emphysematous Patients from Gingival Crevicular Fluid and Sputum Azhary, Mulkan; Amin, Muhammad; Soetjipto, Soetjipto; Mulyadi, Mulyadi; Sunnati, Sunnati
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The elevated activity of matrix metalloproteinase (MMP)-9 has been responsible for degradation of extracellular matrix (ECM) within lung parenchyma leading to emphysema among patientssuffering from chronic obstructive pulmonary disease (COPD). Prolonged exposure of smokinghad triggered activation of both MMP-9 and MMP-3. Active MMP-3 might degrade numerous kinds of ECM and act as MMP-9 inducer as well. The study aimed to correlate active MMP-3 towards elevated MMP-9 activity from both gingival crevicular fluid (GCF) and sputum to assess breakage of extracellular matrix (ECM) among emphysematous patients. Fifteen emphysematous respondents suffering from COPD were recruited to undergo thoroughly physical assessment, spirometry, and radiological examination. Then, both GCF and sputum were collected for measurement of MMP-3 and MMP-9 activity. Results showed that MMP-3 activities were correlated positively and significant with elevated MMP-9 activities from both GCF and sputum i.e. r = 0.899 (p<0.05) and r = 0.770 (p<0.05) respectively. Smoking exposure released many radicals and oxidants generating elevation of MMP-3 activity which then influenced repeatedly influx of neutrophils and activation of MMP-9. The role of active MMP-3 also involved in either acute inflammatory or broad ECM breakage. Moreover, active MMP-9 might lead mainly the degradation of ECM within lung parenchyma. Because of similar effect and impact concerning ECM degradation, both active MMP-3 and MMP-9 might concurrently cause larger breakage of ECM leading to lung emphysema among COPD patients. This study showed that both GCF and sputum would be assigned to evaluate active MMP-3 and MMP-9 for assessing ECM degradation among emphysematous patients.
ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.
PEMBELAJARAN GEOGRAFI MELALUI PENDEKATAN JAS UNTUK MENGEMBANGKAN KECAKAPAN HIDUP Soetjipto, Soetjipto
Pendidikan Geografi (Berkala) Vol 16, No 1 (2011)
Publisher : Universitas Negeri Malang

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Abstract

Abstrak: Pendidikan kecakapan hidup merupakan salah satu upaya peningkatan mutu pendidikan. Esensi pendidikan kecakapan hidup adalah untuk meningkatkan relevansi pendidikan dengan nilai-nilai kehidupan nyata, baik preservatif maupun progresif. Relevansi antara keduanya mengarahkan pada pendidikan yang lebih bersifat realistis dan kontekstual. Bentuk pembelajaran geografi JAS berpotensi dan ber kontribusi untuk mengembangan kecakapan hidup peserta didik. Hal ini dikarenakan karakteristik dan komponen pendekatan JAS dapat mendukung pengembangan domain utama dalam kecakapan hidup, baik domain kecakapan hidup umum (general) maupun kecakapan hidup khusus (spesifik). Keberhasilan pengembangan kedua domain kecakapan hidup peserta didik melalui pembelajaran di kelas sangat tergantung pada kreativitas guru dalam mendesain bentuk pembelajaran bagi peserta didik.Kata Kunci: Pembelajaran, Pendekatan JAS, Pendidikan Kecakapan Hidup
ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.
Uji Coba Vaksin Dengue Rekombinan pada Hewan Coba Mencit,Tikus, Kelinci dan Monyet Soegijanto, Soegeng; Rantam, Fedik A; Soetjipto, Soetjipto; Sudiana, Ketut; Priyatna, Yoes
Sari Pediatri Vol 5, No 2 (2003)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

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Abstract

Pencegahan terhadap infeksi dengue dengan cara vaksinasi perlu dikembangkan; olehkarena secara epidemiologi infeksi virus dengue telah menyebar ke daratan Asia, Afrika,Amerika dan Eropa. Pendekatan pencegahan dan pemberantasan dengan melakukanpemberantasan vektor tidaklah cukup untuk menekan angka kesakitan. Pengembanganvaksin dengan menggunakan protein E dapat menginduksi produksi antibodi terhadapsemua strain (galur) virus dengue.Tujuan penelitian menentukan daya proteksi antibodi yang dipacu oleh calon vaksinpada hewan percobaan (mencit, tikus, kelinci dan monyet).Metode penelitian Isolasi virus dari pasien DBD di RS Dr. Sutomo Surabaya dan isolatstandar dari NAMRU-2 Jakarta. Dilakukan purifikasi isolat virus dengue dan purifikasiprotein E rekombinan. Selanjutnya dilakukan imunisasi pada binatang percobaan dandinilai respon imunnya.Hasil penelitian karakterisasi dan identifikasi imunoglobulin dari mencit yang diimunisasidengan protein E selain IgM, IgG ditentukan subklas IgG1a, IgG2a, IgG2b. Protein Epada hewan percobaan dapat menginduksi antibodi humoral dengan berbagai kelasimunoglobulin maupun subkelasnya dan antibodi seluler yang protektif. Analisis hasilrespon imun pada CD 4 dan CD 8 yang di isolasi dari hewan percobaan yang telahdiimunisasi, direuksikan dengan IFN-¶ ternyata menunjukkan adanya perbedaan responimun yang berbeda. Pada challenge test hanya monyet yang memberikan respons patologisyaitu terlihat adanya perdarahan pada hari ke tiga setelah infeksi. Protein E yangdiimunisasikan pada monyet dapat menginduksi antibodi humoral dengan titer cukuptinggi terutama imunoglobulin G.
Pengembangan Perangkat Pembelajaran IPA Model Inkuiri Terbimbing (Guided Inquiry) untuk Meningkatkan Pemahaman Konsep Siswa SMP Hermanto, Fery; Soetjipto, Soetjipto; Hidayat, M. Thamrin
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol 4, No 2: December 2016
Publisher : IKIP Mataram

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Abstract

The objective of this research to produced learning materials were valid, practical, and effective to improved student’s conceptual understanding. The development of learning material used the ADDIE (Analyze, Design, Develop, Implement, and Evaluate) model and was tried out at VIII-A and VIII-B on SMP Negeri 1 Bandung Tulungagung with One Group Pretest-Posttest Design. Data were collected by used observation method, tests, and questionnaires. Data was analyzed descriptive analysis quantitatively and qualitatively. The results showed that 1) the learning material are valid categorized; 2) learning material in terms of practical category in (a) feasibility of instruction good category, (b) Obstacles for learning could be overcome, (c) student’s activity was active category; (3) the learning material effectiveness in terms of; (a) The response of student’s positively, and (b) student’s conceptual understanding increased significant after learning material was tried out. It could be concluded that the intervention design was valid, practical and effective to improved student’s learning achievement.
Effects of Robusta coffee (Coffea canephora) brewing on levels of RANKL and TGF- β1 in orthodontic tooth movement Herniyati, Herniyati; Narmada, Ida Bagus; Soetjipto, Soetjipto
Dental Journal (Majalah Kedokteran Gigi) Vol 49, No 3 (2016): (September 2016)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Orthodontic tooth movement will be followed by periodontal ligament and alveolar bone remodeling. Orthodontic mechanical force (OMF) will be distributed through the teeth to periodontal ligament and alveolar bone and then will generate local pressure resulting in bone resorption and tension areas that will form new bone. Robusta coffee contains caffeine, chlorogenic acid and caffeic acid. Caffeine may increase osteoclastogenesis, and caffeic acid has antioxidant effects that may reduce oxidative stress in osteoblasts. Purpose: This study conducted to analyze the effect Robusta coffee steeping on levels of RANKL and TGF-β1 in orthodontic tooth movement. Method: 16 male rats were divided into 2 groups. Group C: rats given OMF, Group T: given OMF and coffee brew at 20 mg/ 100 g BW. OMF in rats was conducted by applying ligature wire on the molar-1 (M-1) and both incisivus of right maxilla. Subsequently, M-1 of right maxilla was moved to mesial with a Niti closed coil spring. Observations were made on days 15 and 22 by taking the GCF by putting paper point on the gingival sulcus of mesio- and disto-palatal areas of M-1 of right maxilla to determine the levels of RANKL and TGF-β1 using ELISA method. Result: The administration of coffee brew was effective to increase levels of RANKL and TGF-β1 in the compression and tension areas (p <0.05). RANKL levels in compression area were higher than in the tension area (p <0.05), while the levels of TGF-β1 in the tension area were higher than in the compression area (p <0.05). Conclusion: The administration of coffee brew was effective to increase the levels of RANKL and TGF-β, therefore it might improve alveolar bone remodeling process.
Penelitian konversi kulit domba, kambing krom basah menjadi kulit jaket, glase ditinjau dari parameter prosesnya sebagai produk perdagangan Soetjipto, Soetjipto
Majalah Kulit, Karet, dan Plastik Vol 7, No 12-13 (1992): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

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Abstract

The object of this research is to determine mutual converted value between unfinished leather to finished leather as commercial commodity. The raw materials are wet chrome tanned goatskins which are converted into jacket leather and chrome tanned goatskins into glace leather. Weight, wide, thickness is measured during this process, and the last is carried out in accordance with Indonesia Industrial Standard, for each step of the process. The sample taken in accordance with ISO. 2588 1973 is n = 0,5 √N, where N is the number of cattle slaughtered. The sheeps taken were 148 sheets (197 sheets available) and the goats taken were 130 sheets (195 sheets available). The conclusion of this research are the conversion value of some sheepskins and goatskins from chrome tanned, sheepskins into glace  and goatskins into jacket. INTISARI Penelitian ini bertujuan untuk menentukan nilai konversi hubungan timbal balik antara tahap sebelum dan sesudah pengolahan kulit sesuai produk perdagangan. Dimulai dari kulit krom basah, menjadi kulit jaket untuk domba dan menjadi kulit glase untuk kulit kambing. Dilakukan pengukuran terhadap  berat, luas dan tebal. Cara pengambilan sampel menurut ISO. 2588-1975. Jumlah sampel adalah n = 0,5 √N, dimana N = jumlah ternak yang dipotong, sehingga sampel untuk domba = 130 lembar diperoleh 195 lembar, untuk kulit kambing 148 lembar diperoleh 197 lembar. Dari pengolahan data dapat disimpulkan, beberapa harga konversi kulit domba dan kulit kambing dimulai dari kulit krom basah sampai kulit jaket dan kulit glase.