Yuliana Heri Soesilo
Fakultas Kedokteran, Universitas Sebelas Maret

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Polimorfisme Gen Ferroportin (FPN1) Q248H dan Karakteristik Sosial Ekonomi Ibu Hamil dengan Anemia di Surakarta Istiqomah, Nor; Dewi, Vitria Sari; Farmawati, Arta; Sadewa, Ahmad Hamim; Soesilo, Yuliana Heri; Damayanti, Kusumadewi Eka; Indarto, Dono
Pena Medika Jurnal Kesehatan Vol 4, No 1 (2013): Pena Medika Jurnal Kesehatan, Vol.4 No.1 Desember 2013
Publisher : Fakultas Ilmu Kesehatan Universitas Pekalongan

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Abstract

Background: Iron deficiency anemia in pregnancy remains an important health problem in Indonesia. Ferroportin (FPN1) is one of important gene in iron metabolism encodes ferroportin for iron absorption, release, and recycle inside the body. The polymorphism of FPN1 Q248H alters negative charge of the amino acid sequence positively, hence influenced its ubiquitination and degradation. Beside of that, the high prevalence of anemia not only caused by genetics, nutrition, physiology of each pregant women, but also demographic factor and sosio-economic burden of family. This research will study the polymorphism of ferroportin Q248H and demographic character in pregnant women in Surakarta. Methods: The study using descriptive analytic case-control approach. The data used were from questionaire and blood sampling whose counted for screening anemia in private laboratorium. Pregnant women who had intention and had 10-25 week in pregnant were selected to be research subject.  FPN1 Q248H polymorphism were determined using PCR-RFLP method. Analysis Chi-square dan Independent t-test used to conclude the relationship between each variable to anemic state of pregnant women in Surakarta. A p<0.05 was considered as significant. The study using decriptive analytic design method. Results: Prevalence of anemia in Surakarta is 25.7% with 80.7% have mild anemia. There was no varian in FPN1 Q248H. Majority of pregnant women in group of anaemia have demographic character like 20-25 old, multigravid, last education in High School, housewife, their income range from Rp 500.000,00 to Rp 1.000.000,00, and consume Fe tablet. The bivariat analysis show no statistical significancy of parity, maternal age, education, income, and comsumption of iron tablet to state of anaemia (p>0.05). Conclusions: The FPN1 Q248H polymorphism was not a risk factor for iron deficiency anemia in pregnant women, likewise socio-economic factors had no significant role to incidence of anemia in Surakarta. Keywords: Anemia in pregnancy, Ferroportin (FPN1) Q248H polymorphism, Socio-Economic
Polimorfisme gen ferroportin (FPN1) -1355 G/C sebagai faktor risiko anemia defisiensi besi pada ibu hamil Istiqomah, Nor; Umarghanies, Sarah Safira; Farmawati, Arta; Sadewa, Ahmad Hamim; Soesilo, Yuliana Heri; Damayanti, Kusumadewi Eka; Indarto, Dono
Jurnal Gizi Klinik Indonesia Vol 9, No 4 (2013): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.17 KB) | DOI: 10.22146/ijcn.18364

Abstract

Background: According to WHO data, prevalence of anemia pregnancy in Indonesia is 44.3%, it’s higher than world prevalence (41.8%). Ferroportin (FPN1) is one of important iron exsporter for iron absorption, release, and recycle inside the body. The varian of FPN1-1355 G/C in promoter region, leads to increased of ferroportin expression and iron export, increased cellular iron needs, overexpression of soluble transferrin receptor (sTfR), decrease hemoglobin (Hb) and erythrocyte indices that manifest to iron deficiency anemia (IDA).Objective: This research will study the frequency of FPN1-1355 G/C polymorphism as a risk factor of IDA in pregnant women in Indonesia.Method: The research design was a case and control study. Blood samples were taken from 26 pregnant women with anemia and 48 pregnant women without anemia. FPN1-1355G/C polymorphism were determined using PCR-RFLP method. sTfR and ferritin level were measured with ELISA. Hemoglobin, erythrocyte indices, and sTfR level were compared among genotype group, then statistically analyzed using independent sample t-test and one way ANOVA. Bivariat analysis of Pearson test was conducted to analyze correlation between level of blood Hb and ferritin in pregnant women (p<0.05).Results: FPN1-1355 G/C polymorphism with frequency in pregnant women with IDA and in pregnant women with anemia non IDA were 100% and 95.2%, respectively (p=0.710; OR=1.600; 95%CI: 0.296-8.653). The mean of Hb level and erythrocyte indices in subjects carrying C allele were lower than subjects carrying only G allele although Hb level is not significantly different (p>0.05). The sTfR and hepcidin level in subjects carrying C allele were higher than subjects carrying only G allele (p<0.05). Conclusion: In this study the FPN1 gene promoter -1355 G/C polymorphism was not a risk factor for anemia, but it was a risk factor for iron deficiency anemia in pregnant women.
Polimorfisme gen ferroportin (FPN1) -1355 G/C sebagai faktor risiko anemia defisiensi besi pada ibu hamil Istiqomah, Nor; Umarghanies, Sarah Safira; Farmawati, Arta; Sadewa, Ahmad Hamim; Soesilo, Yuliana Heri; Damayanti, Kusumadewi Eka; Indarto, Dono
Jurnal Gizi Klinik Indonesia Vol 9, No 4 (2013): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.18364

Abstract

Background: According to WHO data, prevalence of anemia pregnancy in Indonesia is 44.3%, it’s higher than world prevalence (41.8%). Ferroportin (FPN1) is one of important iron exsporter for iron absorption, release, and recycle inside the body. The varian of FPN1-1355 G/C in promoter region, leads to increased of ferroportin expression and iron export, increased cellular iron needs, overexpression of soluble transferrin receptor (sTfR), decrease hemoglobin (Hb) and erythrocyte indices that manifest to iron deficiency anemia (IDA).Objective: This research will study the frequency of FPN1-1355 G/C polymorphism as a risk factor of IDA in pregnant women in Indonesia.Method: The research design was a case and control study. Blood samples were taken from 26 pregnant women with anemia and 48 pregnant women without anemia. FPN1-1355G/C polymorphism were determined using PCR-RFLP method. sTfR and ferritin level were measured with ELISA. Hemoglobin, erythrocyte indices, and sTfR level were compared among genotype group, then statistically analyzed using independent sample t-test and one way ANOVA. Bivariat analysis of Pearson test was conducted to analyze correlation between level of blood Hb and ferritin in pregnant women (p<0.05).Results: FPN1-1355 G/C polymorphism with frequency in pregnant women with IDA and in pregnant women with anemia non IDA were 100% and 95.2%, respectively (p=0.710; OR=1.600; 95%CI: 0.296-8.653). The mean of Hb level and erythrocyte indices in subjects carrying C allele were lower than subjects carrying only G allele although Hb level is not significantly different (p>0.05). The sTfR and hepcidin level in subjects carrying C allele were higher than subjects carrying only G allele (p<0.05). Conclusion: In this study the FPN1 gene promoter -1355 G/C polymorphism was not a risk factor for anemia, but it was a risk factor for iron deficiency anemia in pregnant women.