Marsetyawan HNE Soesatyo
Bagian Histologi dan Biologi Sel, Fakultas Kedokteran, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Hubungan Produksi IFN- dan IL-4 dengan Pengobatan Strategi DOTS fase intensif pada Penderita Tuberkulosis Paru Indreswari, Sri Andarini; Hadisaputro, Suharyo; Soesatyo, Marsetyawan HNE; Dharmawan, Yudhy
PROSIDING SEMINAR NASIONAL Vol 1, No 1 (2008): CONTINUING MEDICAL AND HEALTH EDUCATION (CMHE) | Peran Biomolekuler dalam Pen
Publisher : Universitas Muhammadiyah Semarang

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Latar Belakang: Tuberkulosis paru masih menjadi masalah utama di seluruh dunia, terutama di Negara sedang berkembang. Di Indonesia hasil pengobatan dan konversi belum optimal. Banyak penyebab kekurang berhasilan pengobatan ini belum diketahui, khususnya yang berkaitan dengan faktor imunologi. Penelitian ini bertujuan menjelaskan hubungan produksi IFN- dan IL-4 dengan kesembuhan klinis, dalam hal ini terjadinya konversi BTA pasca 2 bulan pengobatan dengan strategi DOTS.Metoda: Rancangan penelitian adalah nested case control, pada penderita baru tuberkulosis paru dengan pemeriksaan sputum BTA positip yang mendapat pengobatan strategi DOTS selama 2 bulan. Kasus adalah penderita yang tidak mengalami konversi pasca 2 bulan pengobatan (BTA tetap positip), sedangkan kontrol adalah penderita yang mengalami konversi pasca 2 bulan pengobatan (BTA menjadi negatip). BTA sebagai hasil pemeriksaan Ziehl Neelsen yang diteruskan dengan tes Niasin. Produksi IFN-dan IL-4 di dalam serum diperiksa dengan metode ELISA. Untuk uji beda rata-rata produksi sitokin antara kasus dan kontrol dilakukan analisis dengan T- test.Hasil: Jumlah sampel 73, diperoleh dari 158 penderita baru berobat jalan yang diikuti selama 2 bulan, terdiri dari 34 kasus (14 diperiksa sitokin) dan 39 kontrol (21 diperiksa sitokin). Penelitian dilakukan di BP4, 12 Puskesmas dan RSUD Kota Semarang. Produksi rata-rata IFN- di dalam serum pasca 2 bulan pengobatan berbeda secara signifikan antara kasus dan kontrol dengan stimulasi PPD 0,5 ug/mL dan PPD 5 ug/mL. Tidak terdapat perbedaan antara kasus dan kontrol pada produksi rata-rata IL-4 dengan semua stimulasi dan tanpa stimulasi.Simpulan: Perbedaan secara signifikan antara kasus dan kontrol pasca 2 bulan pengobatan dalam produksi sitokin (IFN-) bersifat spesifik (hanya dengan stimulasi antigen). Produksi IL-4 tidak terdeteksi kecuali dengan stimulasi PHA, tidak terdapat perbedaan secara signifikan antara kasus dan kontrol.Kata kunci: Tuberkulosis, Interferon-, Interleukin-4, DOTS
PERAN TEMPE KEDELAI HITAM DALAM MENINGKATKAN AKTIVITAS ENZIM ANTIOKSIDAN DAN DAYA TAHAN LIMFOSIT TIKUS TERHADAP HIDROGEN PEROKSIDA IN VIVO Nurrahman, -; Astuti, Mary; Suparmo, -; Soesatyo, Marsetyawan HNE
PROSIDING SEMINAR NASIONAL 2012: SEMINAR NASIONAL HASIL PENELITIAN 2012
Publisher : Universitas Muhammadiyah Semarang

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Konsumsi tempe dapat meningkatkan status antioksidan dalam tubuh karena tempe kedelaihitam mengandung senyawa antioksidan. Tujuan dari penelitian ini mengkaji peran tempekedelai hitam dalam meningkatkan aktivitas enzim antioksidan plasma dan daya tahan limfositterhadap hidrogen peroksida in vivo. Sebanyak 24 ekor tikus dikelompokan menjadi 4 (empat).Keempat kelompok sebanyak 6 ekor tikus diperlakukan dengan pemberian diit standar, diitditambah tepung tempe kedelai hitam, diit ekstrak tempe kedelai hitam dan diit kombinasitepung dan ekstrak tempe. Pada hari ke-36 tikus dimatikan untuk diambil darah dan limpa.Darah dibuat plasma, yang kemudian digunakan untuk analisa aktivitas enzim superoksidadismutase (SOD), katalase dan glutation peroksidase dan limpa diekstrak limfositnya untukanalisa daya tahan limfosit terhadap hidrogen peroksida (90 M). Hasil penelitian menunjukkanadanya peningkatan aktivitas enzim SOD dan daya tahan limfosit terhadap hidrogen peroksidasecara signifikan. Kesimpulan dari penilitian ini bahwa konsumsi tempe kedelai hitam dapatmeningkatkan status antioksidan tikus.
Faktor HLA-DRB pada Penderita Tuberkulosis Paru dengan Pengobatan Strategi DOTS Indreswari, Sri Andarini; Hadisaputro, Suharyo; Soesatyo, Marsetyawan HNE; Handono, Kusworini
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 1 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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ABSTRACTHLA-DRB factor in pulmonal tuberculosis with DOTS strategic treatmentBackground: Tuberculosis remains one of the world’s greatest public health problems, especially in developing countries. In Indonesia the results of DOTS strategic treatment and conversion have not been fruitful results. Many factors play important roles in the success of DOTS strategic treatment, but a little attention was given to the immuno genetics aspects. This study was aimed at theassociation between HLA-DRB factors and clinical output on DOTS strategic treatment (after first 2 months of treatment):conversion of sputum smear positive.Method: A nested case control study was carried out. The exposure variables were alleles of HLA-DRB (result of PCR examination), while the independent variables were sputum smear positive and negative (result of laboratories examination with Ziehl Neelsen staining, Niacin test). Body mass index (BMI) and sex were confounding variables. Odds ratio (OR) was calculated using bivariate and logistic regression for multivariate analysis.Result: A total sample of 73 new patients with active tuberculosis (sputum smear positive) in developing treatment with DOTS strategic treatment, consist of 34 cases and 39 controls. The odds ratio (OR) of HLA-DRB1*1502 and HLA-DRB5*01 were 3.2 (95%CI: 1.103-9.287). The OR of HLA-DRB1*1201 was 0.305 (95% CI: 0.117-0.798), OR of HLA-DRB3*01 was 0.214 (95% CI: 0.077- 0.592). The PAR (population attributable rate) of HLA-DRB1*1502 and HLA-DRB%*01 were 42.64%. While confounding variables were analyzed, only allele HLA-DRB1*1502 was significant, OR 4.9 (95% CI: 1.234-15.617), the probability was 70.57%.Conclusion: HLA-DRB1*1502 is an allele is a risk factor for the conversion of sputum smear positive after 2 months of treatment.Keywords: Tuberculosis, human leukocyte antigen (HLA)ABSTRAKLatar belakang: Di Indonesia hasil pengobatan  tuberkulosis paru belum optimal. Penyebab kekurangberhasilan pengobatan ini belum diketahui, khususnya yang berkaitan dengan faktor imunogenetika. Penelitian bertujuan menjelaskan hubungan faktor HLADRBdengan kesembuhan klinis, dalam hal ini terjadinya konversi BTA pasca 2 bulan pengobatan dengan strategi DOTS.Metoda: Rancangan penelitian adalah nested case control, pada penderita baru tuberkulosis paru dengan pemeriksaan sputum BTA positif yang mendapat pengobatan strategi DOTS selama 2 bulan. Jenis alel (HLA-DRB) yang ditemukan dengan pemeriksaan PCR dinyatakan sebagai variabel paparan, variabel efek adalah hasil pemeriksaan sputum (BTA) dengan pengecatan Ziehl Neelsen yangditeruskan dengan tes Niacin pasca 2 bulan pengobatan. Sebagai variabel perancu ditetapkan BMI dan jenis kelamin. Analisis dilakukan dengan menghitung rasio odds dengan chi-square dan regresi logistik.Hasil: Jumlah sampel 73, diperoleh dari 158 penderita baru berobat jalan yang diikuti selama 2 bulan, terdiri dari 34 kasus (BTA tetap positif pasca 2 bulan pengobatan) dan 39 kontrol (BTA menjadi negatif). Penelitian dilakukan di BP4, 12 puskesmas dan RSUD di Kota Semarang. Hasil penelitian adalah besar risiko (OR) HLA-DRB1*1502 dan HLA-DRB5*01 untuk tidak terjadinya konversi BTA 3,2 (95% CI: 1,103-9,287). Alel HLA-DRB1*1201 dan alel HLA-DRB3*01 merupakan alel yang bersifat protektifdengan OR 0,305 (95% CI: 0,117-0,798), sedangkan HLADRB3*01 dengan OR 0,214 (95% CI: 0,077-0,592). PAR untuk alel HLADRB1* 1502 dan HLA-DRB5*01 sebesar 42,64%. Apabila variabel perancu dimasukkan dalam analisis, maka hanya alel HLA-DRB1*1502 yang secara signifikan merupakan faktor risiko untuk tidak terjadinya konversi BTA pasca 2 bulan awal pengobatan dengan strategi DOTS. OR 4,9 (95% CI:1,234-15,617). Probabilitas untuk HLA-DRB1*1502 adalah sebesar 70,57%.Simpulan: Alel HLA-DRB1*1502 merupakan faktor risikountuk tidak terjadinya konversi BTA pasca 2 bulanpengobatan, dengan probabilitas cukup besar.
The Relationship Between Endothelin-1 and Hypertension on Mlati Population, Sleman, Yogyakarta, Indonesia Aziza, Lucky; Sja’bani, Mochammad; Haryana, Sofia Mubarika; Soesatyo, Marsetyawan HNE; Hamim, Ahmad
Journal of the Indonesian Medical Association Vol. 61 No. 6 June 2011
Publisher : Journal of the Indonesian Medical Association

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Abstract: Hypertension is one of major health problems in the world. The problem has become complicated since blood pressure (BP) is still uncontroled in many populations, including Yogyakarta, Indonesia. Endothelin-1 (ET-1) is a potent vasoconstrictor that has a role in hypertension pathogenesis. In the last decades, some researchers reported relationship between ET-1 and hypertension. The aim of the study is to investigate endothelin-1 expression in hypertensive subjects compared to prehypertensive and normotensive subject in Yogyakarta. This cross-sectional study was performed in 2009. Sample was taken by double random sampling method from 107 normotensive subjects, 97 prehypertensive subjects, and 125 hypertensive subjects. The results showed significant differences of three groups, namely age, body mass index (BMI), family history of hypertension, ET-1 expression and total cholesterol level, in which they tend to have greater value in hypertension group. Mean value of ET-1 in normotension subjects, prehypertension subjects, hypertension subjects were 0.81±0.30; 0.78±0.21 dan 0.81±0.23 respectively. There were no difference between ET-1 and hypertension. It was concluded, in the large-scale homogeneous population in Yogyakarta, the expression of ET-1 in subjects with hypertension is higher compared with prehypertension and normotensive subjects. In addition, in patients receiving captopril regularly, ET-1 expression in subjects with controlled blood pressure was lower than not controlled blood pressure, but the difference was not significant. J Indon Med Assoc, 2011;61: 237-42.Keywords: hypertension, endothelin-1, vasoconstrictor.
The Relationship of Angiotensin-Converting Enzyme Gene Polymorphism Insertion/Deletion and Hypertension in Yogyakarta Population, Indonesia Aziza, Lucky; Sja’bani, Mochammad; Haryana, Sofia Mubarika; Soesatyo, Marsetyawan HNE; Sadewa, Ahmad Hamim
Journal of the Indonesian Medical Association Vol. 60 No. 4 April 2010
Publisher : Journal of the Indonesian Medical Association

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Hypertension is one of major health problem in the world. It becomes complicated since the blood pressure (BP) still uncontrollable, including in Yogyakarta, Indonesia. The reninangiotensin system (RAS) has a central role in controlling BP and sodium homeostasis. RAS polymorphism was defined as genetic determinants of essential hypertension and end-organ damage. One of enzyme that work in RAS, which have a role on hypertension pathogenesis is angiotensin converting enzyme (ACE). ACE convert angiotensin I to angiotensin II. The act of ACE is influenced by ACE gene. In the world, there are many study about the role of ACE gene polymorphism I/D to hypertension. That studies show controversial result. The objective of this study is to evaluate the association between ACE gene polymorphism I/D and hypertension in Yogyakarta population. The method of this study is cross sectional. Sample was taken by double random sampling method from hypertension, prehypertension and normotension, was obtained 125 subjects, 97 subjects dan 108 subjects, consecutively. ACE gene polymorphism I/D was examined by PCR. This study shows significant differences of three groups (ages, body mass index (BMI), and family history of hypertension) and total cholesterol level in blood, which is tend to have greater value in the hypertension group. Frequency of genotype II, ID, DD are 85 (68%), 39(31.2%), 1(0.8%) in hypertension, 66 (61.1%), 38(35.2%), 4(3.7%) in normo-tension and 56(57.7%), 37 (38.1%), 4 (4.1%) in pre-hypertension subject, consecutively. The result of Chi- Square analysis shows statistically significant association between ID+DD vs. II genotype and hypertension. Multiple logistic regression analysis shows four variables that significantly influent to hypertension, namely ages, family history of hypertension, BMI, and ACE gene polymorphism. The conclusion of this study is ACE ID+DD genotype has significant relationship with hypertension in Mlati population, Sleman, Yogyakarta, Indonesia.Keywords: hypertension, genetic, ACE, polymorphism, insertion/deletion
PERAN TEMPE KEDELAI HITAM DALAM AKTIVITAS ENZIM ANTIOKSIDAN DAN DAYA TAHAN LIMFOSIT TIKUS TERHADAP HIDROGEN PEROKSIDA IN VIVO Nurrahman, -; Astuti, Mary; Suparmo, -; Soesatyo, Marsetyawan HNE
PROSIDING SEMINAR NASIONAL Vol 3, No 1 (2010): Sain, Teknologi, Kimia Sosial dan Humaniora, Kimia
Publisher : Universitas Muhammadiyah Semarang

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Konsumsi tempe dapat meningkatkan status antioksidan dalam tubuh karena tempe kedelai hitammengandung senyawa antioksidan. Tujuan dari penelitian ini mengkaji peran tempe kedelai hitamdalam meningkatkan aktivitas enzim antioksidan plasma dan daya tahan limfosit terhadap hidrogenperoksida in vivo. Sebanyak 24 ekor tikus dikelompokan menjadi 4 (empat). Keempat kelompoksebanyak 6 ekor tikus diperlakukan dengan pemberian diit standar, diit ditambah tepung tempekedelai hitam, diit ekstrak tempe kedelai hitam dan diit kombinasi tepung dan ekstrak tempe. Padahari ke-36 tikus dimatikan untuk diambil darah dan limpa. Darah dibuat plasma, yang kemudiandigunakan untuk analisa aktivitas enzim superoksida dismutase (SOD), katalase dan glutationperoksidase dan limpa diekstrak limfositnya untuk analisa daya tahan limfosit terhadap hidrogenperoksida (90 μM). Hasil penelitian menunjukkan adanya peningkatan aktivitas enzim SOD dan dayatahan limfosit terhadap hidrogen peroksida secara signifikan. Kesimpulan dari penilitian ini bahwakonsumsi tempe kedelai hitam dapat meningkatkan status antioksidan tikus.
The Profile of Interferon-γ (IFN-γ) and Interleukin-10 (IL-10) in Pulmonary Tuberculosis Patients Wasityastuti, Widya; Subronto, Yanri W; Soesatyo, Marsetyawan HNE
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Fakultas Kedokteran bekerjasama dengan PETRI

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Introduction: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and becomes the main health problems in the world and in Indonesia, as well. The microorganism itself is an intracellular pathogen. The control of tuberculosis infection depends on cell-mediated immunity involving activated macrophages, T cells, and cytokines. The balance and dynamic changes between Th1 cytokine and Th2 cytokine refl ect the immune response of host and infl uence the clinical manifestation of the disease.Objectives: This research was designed to study the profi le and interaction of IFN-γ (Th1 cytokine) and IL-10 (Th2 cytokine) of pulmonary tuberculosis (PTB) patients in endemic area.Methods: Peripheral blood mononuclear cells of 23 pulmonary TB patients and 16 healthy persons was cultured and stimulated by phytohaemagglutinin (PHA) to investigate the ability to secrete IFN-γ and IL-10.Result: The result showed that there was a decreased of IFN-γ response to PHA in PTB patients, suggesting the defi ciency of general immune capacity in PTB. In contrast, IFN-γ secreted by specifi c antigen was higher in PTB patients which minimal lung lesion was higher than moderate-far advanced. It is related to IFN-γ roles as immunomodulator in cellular immunity and immunoprotectant through stimulated antimicrobial capacity in macrophage. In fact, IL-10 response to PHA and M.tuberculosis antigen in PTB patients was lower than that of in healthy persons; moderate-far advanced lung lesion was the lowest. It was probably refl ecting their poor general conditions. Paired distribution between IFN-γ and IL-10 pointed out the leaning of negative interaction. It refl ected the existence of counterpart/cross regulation between IFN-γ(Th1 cytokine) and IL-10 (Th2 cytokine).Conclusion: In conclusion that specifi c immune response of PTB is related to the degree of lung lesion. This study also provides the balance of Th1 cytokine and Th2 cytokine in relation to TB.Key words: tuberculosis, immune response, IFN-γ, IL-10, lung lesion
Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 Kusumawardani, Banun; Soesatyo, Marsetyawan HNE; Dasuki, Djaswadi; Asmara, Widya
Journal of Dentistry Indonesia Vol 20, No 2 (2013): August
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Trophoblast participates in preventing allorecognition and controlling pathogens that compromise fetal wellbeing. Toll-like receptors recognize conserved sequences on the pathogens surface and trigger effector cell functions. Porphyromonas gingivalis is thought to spread to the umbilical cord and cause fetal growth restriction. Objective: To characterize expression and function of TLR-2 and TLR-4 in trophoblast cells from Porphyromonas gingivalisinfected pregnant rats. Methods: Live Porphyromonas gingivalis were challenged into the maxillary first molar subgingival sulcus of female rats before and/or during pregnancy and sacrified on gestational day (GD) 14 and 20. Porphyromonas gingivalis was detected by API-ZYM system in the maternal blood of the retro-orbital venous plexus and the umbilical cord. TLR-2 and TLR-4 expressions in trophoblast cells was detected by immunohistochemistry. Results: Porphyromonas gingivalis was first detected in the maternal blood and finally spread to the umbilical cord. Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell in treated groups had significantly higher expression of TLR-2 and TLR-4 than control group (p<0.05). Conclusion: Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell are able to recognize Porphyromonas gingivalis through TLR-2 and TLR-4 expression. The ligation of TLR-2 and TLR-4 promoted cytokine production and induced trophoblast cell death. These findings strengthen links between periodontal disease and fetal growth restriction.DOI: 10.14693/jdi.v20i2.150
Gestational Day-Dependent Expression of Interleukin-10 and Tumor Necrosis Factor-alpha in Porphyromonas gingivalis-infected Pregnant Rats Kusumawardani, Banun; Soesatyo, Marsetyawan HNE; Dasuki, Djaswadi; Asmara, Widya
Journal of Dentistry Indonesia Vol 20, No 3 (2013): December
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (926.192 KB) | DOI: 10.14693/jdi.v20i3.199

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Fetal growth restriction remains a major cause of neonatal morbidity and mortality. Porphyromonas gingivaliscan induce placental inflammatory response resulting in fetal growth restriction. Objective: This study aimed to evaluate the potential utility of the pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in rat placental tissues to understand whether these events were causally related. Methods: Female rats were infected with live-Porphyromonas gingivalis at concentration of 2x109 cells/ml into subgingival sulcus area of the maxillary first molar before and/or during pregnancy. They were sacrificed on gestational day (GD)-14 and GD20. The expression of TNF-α and IL-10 in macrophages and trophoblast cells were detected by immunohistochemistry. Results: A higher expression of TNF-α was found in spongiotrophoblast of the Pg-BD group on GD14 (6.30±1.16), and in trophoblastic giant cells of Pg-D group on GD20 (5.50±1.35). Furthermore, a higher expression of IL-10 was found in trophoblastic giant cells of the Pg-BD group on GD14 (4.50±1.51) and in syncytiotrophoblasts of Pg-BD group on GD20 (8.70±2.67). Conclusion: The expression of TNF-α on GD14 and GD20 were accompanied by increased expression of IL-10. The placental pathologic conditions induced by Porphyromonas gingivalis can be inhibited by elevated expression of IL-10 in macrophages and trophoblast cells.DOI: 10.14693/jdi.v20i3.199
THE IGF-1 LEVEL OF ESRD PATIENTS AND ITS RISK FACTORS Hidayati, Titiek; Yuningtyaswari, Yuningtyaswari; Sadewa, Ahmad Hamim; Soesatyo, Marsetyawan HNE
Indonesian Journal of Urology Vol 21, No 1 (2014)
Publisher : Indonesian Urological Association

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Objective: To identify the Insulin-like Growth Factor–1 (IGF-1) level of End Stage Renal Disease (ESRD) and non ESRD populations, and correlation between IGF-1level and ESRD incidences. Material & Method: This case study was carried out in Yogyakarta with 72 volunteers. The cases involved Chronic Kidney Disease (CKD) patients. The controls were non-CKD patients. CKD parameters were established with PERNEFRI diagnostic criteria. Comparison of IGF-1 levels between case and control groups was performed through ANOVA, with confidence level of 95%. Bivariate analysis to identify the correlation between IGF-1 plasma level, smoking status, illness history and body mass index (BMI) by determining odds ratio (OR) of individual risk factor of p < 0.05. Results: We enrolled 72 volunteers, 45 male and 27 female subjects. Of the 45 male patients, 15 CKD and 30 non CKD patients served as cases and controls, respectively. The difference in plasma IGF-1 level was detected in the case and control groups (42.01 ± 10.66 vs. 56.05 ± 24.91) (p < 0.05). The result of bivariate analysis showed passive smoking status, IGF-1 plasma level, DM history and hypertensive illness history had correlation with ESRD incidence with odds ratios of 7.88 (p < 0.005; CI: 1.6-37.5) for passive smokers, 4.3 (p < 0.05, CI: 1.36 to 13.33) for IGF-1 level, 21.5 (p < 0.05; CI) for DM history and 12.4 (p < 0.05; CI: 3.7 to 41) for hypertensive history. Conclusion: There was difference in IGF-1 plasma level between ESRD and non-ESRD patients. The IGF-1 plasma level, passive smoking status, diabetes history, and hypertensive history have correlation with ESRD incidence.Keywords: Insulin-like Growth Factor–1 level, End Stage Renal Disease, case control, odds ratio.