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The Role of of Red Fruit (Pandanus conoideus Lam) Oil towards Colitis Ulcerative Mice Model Khiong, Khie; Soeng, Sylvia; Ratnawati, Hana
Journal of the Indonesian Medical Association Vol. 62 No. 4 April 2012
Publisher : Journal of the Indonesian Medical Association

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Abstract

Introduction: Ulcerative colitis (UC) is chronic inflammation process in gut due to dysregulationof mucous immune systems. Oxidative stress caused by free radicals is believed to aggravate the degree of the disease. Red fruit (Pandanus conoideus Lam.) is indigenous plant from Papua that contains high level of antioxidants. The aim of this research were to examine the role of red fruit oil towards leukocytes proliferation and hystological feature of colon in colitis mice modelMethod: Colitis induced group were divides into 2 gropus; with and without red fruit oil treatment. Red fruit treated groups were given red fruit oil in various dosages of 0,1 ml; 0,2 ml and 0,4 ml respectively. At the end of experiment, all mice were sacrificed and the colons were removed and subjected to histopathological analysis by staining with HE. Lymphocytes were measured using MTT assay method.Result: From histopathological analysis results, 0,1 ml -red fruit oil treated group showed improving colon structure compared to untreated group. Red fruit oil of 0.1 ml and 0.4 ml groups showed increased of non-T leukocytes proliferation significantly compared to untreated group.Conclusion: Red Fruit extract can increase non-T leukocytes and T lymphocytes proliferationsignificantly. J Indon Med Assoc. 2012;62:142-8.Keywords: Red fruit extract, leukocytes and lymphocytes proliferation, Dextran Sulfate Sodium (DSS)
Efek Infusa Daun Pepaya (Carica papaya L.) terhadap Larva Nyamuk Culex sp. Valiant, Michael; Soeng, Sylvia; Tjahjani, Susy
Jurnal Kedokteran Maranatha Vol 9, No 2 (2010)
Publisher : Universitas Kristen Maranatha

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Insidence of filariasis in Indonesia is still increasing and this disease may cause many disabilities. In order to decrease the incidence rate, we can interfere with the life cycle of Culex, the filariasis vector, by using  larvicides.    Most  larvicides  sold  in  the  market  contain  chemical  substances,  such  as  temephos. Therefore, it is necessary to find out more friendly natural larvicides, which are effective but safe; one of them is Papaya Leaves Infusion (PLI). The aim of this research is to find out the effectiveness of PLI as a larvicide  of  Culex  sp.  and  compare  it  with  temephos  powder.  This  research  is  a  real  comparative experimental laboratory study using complete randomized design. 720 Culex’ larvae were divided into 6 treatment  groups,  each  group  was  given  Papaya’s  Leaves  Infusion  1%,  1.5%,  2%,  2.5%,  aquadest (negative  control),  and  temephos  1%  (positive  control).  The  observed  data  were  the  numbers  of  killed larvae within 24 hours. The data were analyzed using one way ANOVA which was continued by  Tukey HSD ( = 0,05). The result showed that PLI 1%, 1.5%, 2%, and 2.5% had very significantly different effects compared to the negative control (p=0.000), but there was no significantly different effect between PLI 2 % and temephos 1%. It is concluded that PLI  2% has a larvicide effect as strong as temephos does.  Keywords : Culex sp larvae, papaya’s leaves infusion, temephos
Imunoekspresi EphB4 pada Karsinoma Endometrium Soeng, Sylvia
Jurnal Kedokteran Maranatha Vol 10, No 2 (2011)
Publisher : Universitas Kristen Maranatha

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Karsinoma endometrium merupakan keganasan invasif pada traktus genitalia wanita yang paling sering terjadi. Ketidakseimbangan hormonal yang salah satu penyebabnya adalah paparan estrogen yang berlebihan, dapat menyebabkan hiperplasia endometrium yang berisiko untuk menjadi karsinoma. Reseptor EphB4 merupakan salah satu subtipe dari reseptor Eph yang termasuk dalam reseptor tirosin kinase. Aktivasi reseptor EphB4 oleh ligannya, ephrin-B2, memegang peranan penting dalam embriogenesis dan morfogenesis berbagai organ dewasa termasuk endometrium, serta pada karsinogenesis berbagai jaringan. Penelitian imunohistokimia dilakukan dengan menggunakan antibodi EphB4 terhadap 30 sampel karsinoma endometrium. Tujuan penelitian adalah untuk mengetahui distribusi dan intensitas imunoekspresi reseptor EphB4 pada karsinoma endometrium diferensiasi baik, sedang, dan buruk, serta hubungan imunoekspresi EphB4 dengan gradasi histopatologis karsinoma endometrium. Hasil penelitian menunjukkan bahwa imunoekspresi EphB4 tampak pada permukaan lumen, membran, dan sitoplasma sel epitel kelenjar endometrium. Distribusi imunoekspresi reseptor EphB4 pada karsinoma endometrium diferensiasi baik, sedang, dan buruk berbeda tidak bermakna, sedangkan intensitas imunoekspresi EphB4 tampak paling kuat pada karsinoma endometrium diferensiasi baik dan paling lemah pada karsinoma endometrium diferensiasi buruk (p<0,01). Intensitas imunoekspresi EphB4 berhubungan terbalik yang bermakna (p<0,01) dengan gradasi histopatologis karsinoma endometrium, semakin lemah intensitasnya, semakin tinggi gradasinya. Simpulan penelitian ini adalah intensitas imunoekspresi EphB4 dapat digunakan sebagai indikator keganasan dan memungkinkan untuk diagnosis dini karsinoma endometrium.Kata kunci: karsinoma endometrium, imunoekspresi reseptor EphB4, gradasi histopatologis.
Free Radicals Scavenger Potency of Betel Leaves (Piper betel L.) Extract and Various Fractions Widowati, Wahyu; Wargasetia, Teresa L.; Khiong, Khie; Mozef, Tjandrawati; Soeng, Sylvia; Risdian, Chandra
Jurnal Kedokteran Maranatha Vol 10, No 1 (2010)
Publisher : Universitas Kristen Maranatha

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The imbalance between free radicals and antioxidants in the biology system can cause various diseases. Betel leaves (Piper betel L.), parts of a medicinal plant, are popularly used as a herbal remedy for diseases, but the scientific basis especially of their antioxidant properties remains unknown. To evaluate free radicals scavenger activity of ethanol extract and various fractions (hexane, ethyl acetate, butanol and water fraction), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and hydroperoxide (H2O2) scavenging activity were determined. To determine the DPPH and H2O2 scavenging activity, betel leaves extract and fractions were prepared in 10 concentrations. The result of this research showed that betel leaves extract and fractions had higher H2O2 scavenger and lower DPPH scavenger activities than gallic acid. The highest DPPH scavenging activity with Inhibitory Concentration (IC50) was found in gallic acid 0.732 μg/mL, ethyl acetate fraction 3.156 μg/mL, and ethanol extract 5.489 μg/mL. The highest H2O2 scavenging activity with IC50 was found in butanol fraction 0.223 μg/mL, gallic acid 0.597 μg/mL, and ethyl acetate fraction 0.783 μg/mL. In conclusion, betel leaves extract and fractions were potential free radicals scavenger; they could be potential candidates to inhibit oxidative stress. Gallic acid and ethyl acetate fraction were the highest free radicals scavenger both in DPPH and H2O2 free radical scavenging activity.Keywords: free radical, betel leaves, DPPH, H2O2, antioxidant
Perbandingan Ekstrak Batang Sereh (Cymbopogon Citratus) dan Citronella Oil Sebagai Repelen terhadap Nyamuk Culex Sp Dewasa Betina Gunawan, Christine Natalia; Tjahjani, Susy; Soeng, Sylvia
Jurnal Kedokteran Maranatha Vol 8, No 2 (2009)
Publisher : Universitas Kristen Maranatha

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Culex is known as the vector transmitter of filariasis and encephalitis, and to control its spread, repellent is commonly used. DEET has been widely used and proven as an effective repellent; however, it also has many side effects that may be harmful to the human health. Accordingly,  people tend to prefer using repellents made of natural herbs, such as lemon grass or citronella oil, which have been proven effective. This research was aimed to compare the effectiveness of lemon grass stem extract, at various concentrations, against citronella oil. This was a real comparative, prospective experimental study using complete randomized design. Seven hundred and fifty Culex sp. mosquitoes were divided into 5 treatment groups; 3 groups were treated with lemon grass stem extract at 50% (EBS50), 75% (EBS75), and 100% (EBS100) concentration; the negative control group was treated with aquadest and the positive control group was treated with citronella oil. An observation was conducted to find out the number of mosquitoes that moved to the opposite side of the glass box. The data were analyzed using one-way ANOVA, followed by Tukey HSD test with α = 0.01. The result showed that citronella oil had the highest effectivity, followed by EBS100, EBS75 and EBS50  (p = 0.000-0.005). The conclusion is that lemon grass stem extract is a Culex mosquito repellent which is less effective  than citronella oil.
Efek Pajanan Timbal terhadap Infertilitas Pria Panggabean, Putri C. T.; Soeng, Sylvia; Ivone, July
Jurnal Kedokteran Maranatha Vol 8, No 1 (2008)
Publisher : Universitas Kristen Maranatha

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Industrial and technological advances have brought a great benefit in human life, but these also bring negative effects to human and environment. Several toxic agents often influence human health, one of them is lead which give toxic effect on male reproductive  system. Lead can cause male infertility through two main mechanisms. First, lead reduces the mannose receptors so that the sperms are unable to conduct the acrosome recation, or cause premature acrosome reaction. Secondly, lead competes with zinc in binding protamine, as the result, it will interfere the chromatin stability of the sperms which are closely related to  male fertility.   
Efek Gelombang Elektromagnetik Telepon Seluler terhadap Spermatozoa Mencit Galur BALB/C Soeng, Sylvia; Wargasetia, Teresa Liliana; Steven, Anna
Jurnal Kedokteran Maranatha Vol 7, No 1 (2007)
Publisher : Universitas Kristen Maranatha

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Nowadays, cellular phones are widely used, but most people seem do not aware of the effect of radio frequency electromagnetic radiation (RFEMR) on human body, especially the male reproduction system.   The objective of this study is to  investigate the biological effects of RFEMR of cellular phone  on sperm  motility and sperm count. Sixteen mice of BALB/c strain were divided into four groups: mice in the control group were not exposed to RFEMR, mice in the the first group were exposed to 20 times/day intensity, the second group to 40 times/day, and the third group to 80 times/day.  Mice were exposed to 1900MHz at specific absorption rate of 96 mW/kg for 7 days. Sperm motility and sperm count  were analyzed by means of Oneway ANOVA using SPSS 13.0. There were highly significant differences of sperm  motility between the exposed mice groups and the control group (p<0.01), but no significant differences among the three exposed groups (p>0.05). There were also highly significant differences of sperm count between the exposed groups and the control group (p<0.01), and the differences among the three exposed group were significant too (p<0.05). It can be concluded that exposure to cellular phone electromagnetic radiation may reduces mice sperm motility and sperm count.
Akurasi Deteksi Mycobacterium tuberculosis dengan Teknik PCR menggunakan “Primer X” dibandingkan dengan Pemeriksaan Mikroskopik (BTA) dan Kultur Sputum Penderita dengan Gejala Tuberkulosis Paru Jasaputra, Diana Krisanti; Onggowidjaja, Philips; Soeng, Sylvia
Jurnal Kedokteran Maranatha Vol 5, No 1 (2005)
Publisher : Universitas Kristen Maranatha

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Akhir-akhir ini, terjadi peningkatan jumlah penderita tuberkulosis. Guna pembe-rantasan penyakit TBC, diagnosis dan deteksi Mycobacterium tuberculosis menjadi amat penting. Deteksi tersebut dapat dilakukan dengan teknik Polymerase Chain Reaction (PCR), pemeriksaan mikroskopik, dan kultur bakteri. Tujuan penelitian ini adalah menilai sensitivitas, spesifisitas, dan akurasi deteksi Mycobacterium tuberculosis dalam sputum penderita TBC paru dengan teknik PCR dibandingkan pemeriksaan secara mikroskopik (Bakteri Tahan Asam/BTA) dan kultur bakteri TBC dalam sputum.Penelitian ini merupakan suatu uji diagnostik yang dirancang secara cross sectional. Penelitian dilakukan terhadap penderita TB paru di BP4 Jl Cibadak Bandung, mulai April 2004 sampai dengan Agustus 2004.Pemeriksaan sputum penderita dilakukan dengan tiga teknik pemeriksaan, yaitu dengan teknik PCR, pemeriksaan BTA secara mikroskopik, dan kultur bakteri.Dibandingkan dengan pemeriksaan Bakteri Tahan Asam secara mikroskospik, deteksi Mycobacterium tuberculosis dengan teknik PCR memiliki sensitivitas 30%, spesifisitas 80%, dan akurasi 47%. Uji kemaknaan dengan Mc Nemar memberikan hasil adanya perbedaan yang bermakna. (p < 0,01).Dibandingkan dengan metode kultur bakteri TBC, deteksi Mycobacterium tuberculosis dengan teknik PCR memiliki sensitivitas 65%, spesifisitas 40%, dan akurasi 57%. Uji kemaknaan dengan Mc Nemar memberikan hasil tidak adanya perbedaan yang bermakna. (p = 1,0). Dibandingkan dengan pemeriksaan Bakteri Tahan Asam secara mikroskospik, deteksi Mycobacterium tuberculosis dengan metode kultur bakteri TBC memiliki sensitivitas 31,6%, spesifisitas 81,8%, dan akurasi 50%. Uji kemaknaan dengan Mc Nemar memberikan hasil adanya perbedaan yang bermakna. (p < 0,01).Dengan demikian, dapat disimpulkan bahwa deteksi Mycobacterium tuberculosis dengan teknik PCR sama baiknya dengan kultur bakteri TBC, namun waktu pemeriksaan dengan teknik PCR lebih singkat dibandingkan dengan kultur bakteri TBC. Mycobacterium tuberculosis banyak tidak terdeteksi dengan pemeriksaan mikroskopik (BTA)
Gambaran Pasien Rawat Inap Diabetic Kidney Disease di Rumah Sakit Immanuel Bandung Atmaharmoni, Widyasanti; Soeng, Sylvia; Evacuasiany, Endang
Jurnal Kedokteran Maranatha Vol 11, No 2 (2012)
Publisher : Universitas Kristen Maranatha

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Sebanyak 5-40% penyandang diabetes dalam 10-15 tahun akan menderita penyulit Diabetic Kidney Disease (DKD) yang berakhir pada end stage renal disease (ESRD). Pasien ESRD dihadapkan pada mortalitas yang cukup tinggi yakni 59-66% dan membutuhkan renal replacement therapy yang menghabiskan biaya cukup besar. Tujuan penelitian ini adalah mengetahui prevalensi DKD dan distribusinya berdasarkan tipe diabetes, kolesterol total dan low density lipoprotein (LDL), tekanan darah, HbA1c, indeks massa tubuh (IMT), dan stadium nefropati. Penelitian ini bersifat observasional deskriptif dengan data retrospektif berupa data rekam medis pasien diabetes melitus yang menjalani rawat inap di Rumah Sakit Immanuel Bandung periode Oktober 2010 hingga September 2011. Variabel berupa jumlah pasien, tinggi dan berat badan, tekanan darah, usia, jenis kelamin, kreatinin, HbA1c, dan profil lipid. Jumlah kasus DKD sebanyak 30 dari 420 kasus diabetes (7,14%). Diabetes tipe 1 sebanyak 70% dan diabetes tipe 2 sebanyak 30%. Kolesterol total dan LDL lebih tinggi pada diabetes tipe 2. Tekanan darah pada 66,67% kasus tergolong hipertensi. Kadar HbA1c lebih dari 6,5% didapatkan pada 80% kasus. IMT tergolong overweight dan obese pada 53,33% kasus. Kasus yang tergolong ESRD sebanyak 13,33%.
The Effect of Mangosteen Peel Extract on Sperm Motility and Sperm Count of Swiss Webster Mice Induced by Hard Physical Exercise Prawira, Ardi; Hasianna, Stella T.; Soeng, Sylvia
Journal of Medicine and Health Vol 1, No 1 (2015)
Publisher : Maranatha Christian University

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High-intensity physical exercises can increase oxidative stress, causing damage to reproductive system. The purpose of this study was to determine the effect of mangosteen peel extract on sperm motility and  sperm count of  Swiss Webster mice induced by high-intensity physical exercise. This was a real experimental with complete randomized design. Twenty five  male Swiss Webster mice were divided into five groups (n = 5). Negative control (KN) was without any treatment, positive control (KP) was given  treadmill exercise only, the treatment groups (KM1, KM2, KM3) were given mangosteen peel extract orally 2, 4, 8 mg respectively as well as treadmill exercises. The treadmill exercises was conducted for 35 days. The sperm motility (mm/second) and the sperm count (per mm3) were assessed manually using Improved Neaubauer chamber. The result were analyzed using one-way ANOVA followed by Tukey HSD. The result showed high significant differences of sperm motility and sperm count between KM2 and KP, also between KM2 with  KM1 and  KM3 (p=0,000). The conclusion was mangosteen peel extract improved the sperm motility and sperm count of Swiss Webster mice induced by high-intensity physical exercise with 4 mg as the optimal dose. Keywords: mangosteen peel, sperm motility, sperm count