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Masculinization on tilapia (Oreochromis sp.) by natural hormone steroid agent from bee resin through artificial diet

Jurnal Akuakultur Indonesia Vol 9, No 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

Masculinization on tilapia ( Oreochromis sp.) has been done with approach of natural hormone steroid agent  from bee resin  through artificial diet before sexual differentiation. This research aim to increase male sex ratio and production performance. Treatment of bee resin dose is 0.6; 1.2; 1.8; 2.4; 3.0 feed ml/kg compared to Control (0 ml/kg diet) were given  during 28 days at larva age 7 day in aquarium, then transferred to the farm ground until age 12 weeks. Average of masculine phenotype percentage increased significantly (P

Phenotype characterization of interspecific hybrid abalone Haliotis asinina and Haliotis squamata seed

Jurnal Akuakultur Indonesia Vol 12, No 1 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT Abalone is one of sea-water aquaculture commodity that having relatively low in growth and survival. Interspesific hybridization between abalone Haliotis asinina and Haliotis squamata is required to produce hybrid seeds having a better phenotype inherited from their parents. Crossbreeding of abalone was done in the reciprocal procedure with a natural spawning technique on mass scale. The hybrid seeds showed higher similarity with female brood (98,69%), while  the larvae from H. squamata × H. asinina were abnormal on trocophore until early veliger phase then dead occurred the next phase. The results showed that hybridization between male H. asinina and female H. squamata had higher fertilization and hatching rate than its reciprocal i.e. 76.01±6.15% and 60.14±4.80%. Keywords: interspesific hybridization, phenotype, Haliotis asinina, Haliotis squamata, abalone  ABSTRAK Abalon merupakan komoditas budidaya laut dengan pertumbuhan yang relatif lambat dan kelangsungan hidupnya rendah. Rekayasa persilangan interspesifik antara abalon Haliotis asinina dan Haliotis squamata diharapkan mampu mengatasi permasalahan benih dan memiliki fenotipe unggul yang diwariskan dari tetuanya. Persilangan abalon dilakukan secara resiprok dengan teknik pemijahan alami skala massal. Hasil penelitian menunjukkan bahwa benih hibrida menunjukkan kemiripan dengan induknya sebesar 98,69%, sedangkan larva hibrida antara jantan H. squamata × H. asinina menunjukkan bentuk abnormal pada fase trokofor hingga veliger awal dan kematian pada fase lanjut. Hibridisasi antara jantan H. asinina dan betina H. squamata memiliki derajat pembuahan dan derajat penetasan yang lebih tinggi dibandingkan resiprokalnya, yaitu berturut-turut 76,01±6,15% dan 60,14±4,80%. Kata kunci: hibridisasi interspesifik, fenotipe, Haliotis asinina, Haliotis squamata, abalon  

Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina

Jurnal Akuakultur Indonesia Vol 12, No 2 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT This study aimed to examine the effect of extract of purwoceng Pimpinella alpina for masculinization of Betta splendens. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L as control. The treatments were given by immersion to 35 embryos at eye spots phase or about 28-hours after fertilization, for eight hours. The immersion process was done for 8 hours. The result showed that 20 µL/L dose of purwoceng extract produced 62.66% male, and hatching rate was 85.71%. This male percentage was higher compared to control (45.91%), but the hatching rate was lower than that of control (98.57%). At the higher dose (30 µL/L), male fish population and hatching rate reduced respectively 39.72% and 68.57%, respectively. Keywords: masculinization, embryo immersion, purwoceng extract, Betta splendens  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pengaruh dosis ekstrak purwoceng Pimpinella alpina extract dalam maskulinisasi ikan cupang hias Betta splendens. Penelitian ini menggunakan metode rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan dosis ekstrak purwoceng. yaitu 10, 20, dan 30 µL/L, serta perlakuan 0 µL/L sebagai kontrol. Perlakuan diberikan melalui perendaman selama delapan jam terhadap 35 embrio saat memasuki fase bintik mata atau sekitar jam ke-28 setelah pembuahan. Hasil penelitian menunjukkan bahwa dosis ekstrak purwoceng 20 µL/L menghasilkan persentase ikan jantan sebesar 62,66%, dan daya tetas telur sebesar 85,71%. Persentase jantan tersebut lebih tinggi daripada kontrol (45,1%), tetapi derajat penetasannya lebih rendah daripada kontrol (98,57%). Pada dosis yang lebih tinggi (30 µL/L), populasi ikan jantan (39,72%), dan derajat penetasan (68,57%) adalah menurun. Kata kunci: maskulinisasi, perendaman embrio, ekstrak purwoceng, Betta splendens

Gracilaria spp. morphology cultured in brackish water pond Pantai Sederhana Village, Muara Gembong

Jurnal Akuakultur Indonesia Vol 13, No 1 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT Gracilaria spp. is a euryhaline species of seaweed which can live in the marine and brackish water. Development of Gracilaria spp. culture in Bekasi is potential because this seaweed can be cultured in ex shrimp pond by polyculture system. The objective of this research was to evaluate the phenotype morphological characteristic of Gracilaria spp. based on and its relationship with shrimp pond water quality. Sampling was done at three shrimp ponds with a salinity range at 13.7–19.2 g/L. Phenotypical characteristics of Gracilaria spp. consisted of colour and thallus morfometrics, while measurement of water quality consisted of physical and chemical charactersof shrimp pond. The result showed that Gracilaria spp. generally had light brown colour. At salinity higher than 13.7 g/kg, the number of secondary thalli increased, the distance among internode tertiary thalli declined, and the number of ramification index increased. Salinity showed a positive correlation with remification index which was 0.571. Keywords: Gracilaria spp., remification index, phenotype, salinity, brackishwater culture  ABSTRAK Gracilaria spp. merupakan spesies rumput laut eurihalin yang dapat hidup di laut dan di perairan payau. Pengembangan budidaya Gracilaria spp. di Bekasi potensial dilakukan karena memanfaatkan tambak bekas budidaya udang dengan sistem polikultur. Penelitian ini bertujuan untuk mengevaluasi karakteristik fenotipe morfologi Gracilaria spp. dan hubungannya dengan kualitas air di tambak budidaya. Sampling dilakukan pada tiga tambak dengan kisaran salinitas 13,7–19,2 g/L. Karakterisasi fenotipe meliputi warna dan morfometrik talus Gracilaria spp., sedangkan parameter kualitas air meliputi karakter fisika dan kimia air tambak. Hasil menunjukkan talus Gracilarias spp. umumnya berwarna coklat muda dan pada salinitas di atas 13,7 g/L menunjukkan jumlah talus sekunder meningkat, jarak internode talus tersier menurun, dan indeks percabangan meningkat (P<0,05). Salinitas berkorelasi positif dengan indeks percabangan sebesar 0,571. Kata kunci: Gracilaria spp., indeks percabangan, fenotipe, salinitas, budidaya air payau

Sex reversal of red tilapia using 17α-methyltestosterone-enriched feed and increased temperature

Jurnal Akuakultur Indonesia Vol 14, No 2 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT The growth rate between male and female red tilapia Oreochromis sp. is different. Generally, the male red tilapia grows faster than the female. Furthermore, the maturation process of red tilapia is relatively fast which causes slower growth rate. One of solutions to this problem is by rearing all male population or mono-sex culture. The method used in this study was commercial feed enrichment with 17α-methyltestosterone at different dosages and water temperature manipulation. The purpose of this research was to examine the effects of commercial feed enrichment with different dosages of 17α-methyltestosterone and water temperature manipulation on success rate of sex reversal of red tilapia into all male population. This research consisted of different temperature treatments (with and without water heating) and 17α- methyltestosterone dosages (0, 10, 20 mg/kg of commercial feed). The best dosage of 17α-methyltestosterone was 20 mg/kg of commercial feed with male to female sex ratio of 86.31%, daily growth rate of 8.18%, and feed conversion ratio of 1.53. In this study, the best treatment to produce the male seeds was the 17α-methyltestosterone treatment. Keywords: 17α-methyltestosterone, sex reversal, red tilapia, temperature  ABSTRAK Ikan nila merah Oreochromis sp. memiliki laju pertumbuhan yang berbeda antara ikan jantan dan betina. Umumnya ikan nila merah jantan lebih cepat tumbuh dibandingkan betinanya. Selain itu, ikan nila memiliki sifat cepat matang gonad dan mudah memijah sehingga akan menghambat pertumbuhan ikan. Salah satu cara untuk mengurangi masalah yang terjadi yakni dengan memelihara populasi ikan nila merah tunggal kelamin atau monoseks jantan. Metode yang dilakukan adalah pemberian hormon 17α-metiltestosteron dengan dosis berbeda melalui pakan buatan dan peningkatan suhu air. Penelitian ini bertujuan untuk mengetahui pengaruh dosis hormon 17α-metiltestosteron melalui pakan buatan dan peningkatan suhu air terhadap keberhasilan alih kelamin jantan pada ikan nila merah. Penelitian ini terdiri atas perlakuan suhu (dengan dan tanpa pemanasan air) dan dosis 17α-metiltestosteron (0, 10, 20 mg/kg pakan). Dosis hormon 17α-metiltestosteron terbaik yang didapatkan adalah 20 mg/kg pakan dengan nisbah kelamin jantan 86,31%, laju pertumbuhan harian 8,18%, dan rasio konversi pakan 1,53. Pada penelitian ini perlakuan terbaik untuk menghasilkan benih jantan adalah perlakuan dosis 17α-metiltestosteron. Kata kunci: 17α-metiltestosteron, alih kelamin, ikan nila merah, suhu

Effect of human chorionic gonadotropin on gonadal development of female striped snakehead

Jurnal Akuakultur Indonesia Vol 14, No 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

ABSTRACT This experiment was carried out to confirmed the role of human chorionic gonadotropin (hCG) injection on the gonadal development of striped snakehead (Channa striata, Bloch) reared in controlled tank. Two groups of female broodstocks were used as control and as treated fish. Fishes were fed with tilapia seed twice daily at satiation. The injection of hCG at the dose of 500 IU/kg body weight was done intramuscular once a week in three weeks of rearing period, whereas physiological solution was injected to control fishes. After one week, fishes injected by 500 IU/kg hCG had more big-size oocytes (0.8–1.2 mm) than control. There was no significant difference (P>0.05) on percentage of gonadal maturity index (GMI), hepatosomatic index (HIS), fecundity, and specific growth rate (SGR) between both treatments. The study showed that the injection of hCG at the dose of 500 IU/kg body weight with three times injection could accelerate the oocytes development of female striped snakehead. Keywords: hCG, gonadal development, striped snakehead fish, Channa striata  ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh penyuntikan hormon human chorionic gonadotropin (hCG) terhadap perkembangan gonad ikan gabus (Channa striata, Bloch) yang dipelihara dalam wadah terkontrol. Dua kelompok induk betina digunakan dalam penelitian sebagai kontrol dan ikan perlakuan. Induk diberi pakan benih ikan nila secara at satiation dua kali sehari. Penyuntikan hormon HCG dengan dosis 500 IU/kg dilakukan secara intramuskuler seminggu sekali selama tiga minggu pemeliharaan sedangkan penyuntikan larutan fisiologis dilakukan pada ikan kontrol. Mulai minggu kedua, ikan yang disuntik hormon HCG dengan dosis 500 IU memiliki jumlah oosit berukuran besar (0,8–1,2 mm) yang lebih banyak dibanding ikan kontrol. Nilai indeks kematangan gonad (IKG), indeks hepatosomatik (HIS), fekunditas, dan laju pertumbuhan spesifik (LPH) antara ikan kontrol dan perlakuan hCG menunjukkan nilai yang tidak berbeda nyata (P>0,05). Berdasarkan hasil penelitian, penyuntikan hormon hCG dengan dosis 500 IU/kg dengan frekuensi penyuntikan tiga kali dapat mempercepat perkembangan oosit ikan gabus betina. Kata kunci: hCG, perkembangan gonad, ikan gabus, Channa striata

Masculinization on tilapia (Oreochromis sp.) by natural hormone steroid agent from bee resin through artificial diet

Jurnal Akuakultur Indonesia Vol 9, No 2 (2010): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (134.02 KB)

Abstract

Masculinization on tilapia ( Oreochromis sp.) has been done with approach of natural hormone steroid agent  from bee resin  through artificial diet before sexual differentiation. This research aim to increase male sex ratio and production performance. Treatment of bee resin dose is 0.6; 1.2; 1.8; 2.4; 3.0 feed ml/kg compared to Control (0 ml/kg diet) were given  during 28 days at larva age 7 day in aquarium, then transferred to the farm ground until age 12 weeks. Average of masculine phenotype percentage increased significantly (P

KERAGAAN HIBRIDAINTRA SPESIFIK DARIEMPAT STRAIN IKAN NILA (Oreochromis niloticus) DIKARAMBA JARING APUNG, DANAU LIDO, BOGOR

BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

The objectives of the study was to evaluate hybrid performance of sixteen reciprocal cross resulted from three strains (Red NIFI,Nirwana, BEST) and one population (Lido Lake population) of nile tilapi. The study was conducted in experimental floating netcages in Lido Lake. Fish of 25 days old ranged 0,4 ± 0.07 g were used in the experiment. Fish were reared in floating net cages sized 2x1x1 m for each cross with the density of 50 fish/cage for eight weeks (56 days). Feed were given 10% of body weight with frequency three times a day. The results showed that the highest average larvae production was 1216 from Nirwana female crossed with four different male. Growth rate of hybrids were higher than the parents (P < 0.05) and the highest was .ed NIFI x c? BEST (6,440±0,0712% ).The highest survival rate was $ Red Lido x S BEST hybrid (77.0 ±2,78%")). For heterosis, Specific Growth Rate (SGR) was 5.8188% on hybrid between Nirwana and Red Lido. While for survival rate was 15.5888% on Nirwana x BEST hybrid. Crossing between fenotipe of light color background (red) and dark color background (black) showed that red color was dominant to the black one. Pleiotric effect of color gen was detected on the SGR and survival rate.

RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements]

BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.

ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS [Channastriata(Bloch, 1793)] [Analysis of Genotype Variation and Truss Morphometricof Three Populations of Snakehead Fish [Channa striata (Bloch, 1793)]

BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.