Sri Angky Soekanto
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430

Published : 6 Documents
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THE EFFECT OF OESTROGEN AND OR CALCIUM VITAMIN D3 ON THE MANDIBULAR HEIGHT OF POST OVARIECTOMIZED WISTAR RATS

Journal of Dentistry Indonesia Vol 10, No 1 (2003): APRIL
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

One of the major health problems in elderly women is osteoporosis post menopause. Dentists must be aware of the disease since its involvement on the jaw. Nowadays, there are a lot of researches on correlation of osteopororsis and mandible but only few concentrate on hormonal substitution therapy and/or Calcium-vitamin D3 (Ca-Vit D3). This research is to evaluate the effect of hormonal substitiution therapy and/or Ca-vit D3 on mandibular height. Forty five rats used in this research and divided into nine groups: one control group, two ovariectomized (OVX) groups, two OVX groups treated with estrogen, two OVC groups treated with Ca-vit D3, two OVC groups treated with estrogen and Ca-vit D3. All of the rats except the control group were ovariectomized as model for postmenopausal estrogen deficiency state. The treatment was done in two or four weeks. The animals were killed with cervical dislocation, the mandible were excised and soaked on Hydrogen Peroxide 10%. Then the mandibles heights on right buccal side were measured from the manduble base to the alveolar crest. It is concluded that hormonal substitution therapy and combination of the hormonal substitution therapy and Ca-vit D3 can maintain the normal mandibular height. Mandibular height of groups with therapy using Ca-vit D3 have slightly lower means compared to control group but without significant difference statistically. The best therapy is combination of hormonal substitution therapy and Ca-vit D3.

PENGARUH TERAPI SULIH HORMON ESTROGEN, PREPARAT KALSIUM DAN KOMBINASINYA PADA TULANG MANDIBULA (Studi eksperimental laboratorik pada tikus Wistar setelah ovariektomi)

Journal of Dentistry Indonesia Vol 10, No 2 (2003): AUGUST
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

The purpose of this study was to evaluate the effects of ovariectomy, the influences of estrogen replacement therapy and/or suppelment CavitD3 on mandibles calcium contents of animals models. Forty-five female rats were used in this research. Five rates were sacrificed for unoperated basal controls and the others were bilaterally ovariectomized. The ovariectomized animals were divided into 8 groups and two of these groups were only ovariectomized, the others were treated by estrogen replacement therapy. CavitD3 and combination of both per oral for 2 and 4 weeks and then were sacrificed. Mandibles calcium contents were analyzed by Ion Selective Electrode after demineralized with 10% phosphoric acid for 24 hours. Result : there were significant increased of mandibles calcium content at 2 weeks post-ovariectomy, but the results decreased at 4 weeks post-ovariectomy. Estrogen replacement therapy and treated with CavitD3 for 2 and 4 weeks or combination of both for 2 weeks increased the mandibles calcium contents of ovariectomized rats, but therapy combination with estrogen and CavitD3 for 4 weeks had a protective effect to mandibles calcium contents of ovariectomized rats.

Surface Structure Study of Crystal Hydroxy-Apatite from Fluorosis Enamels

Journal of Dentistry Indonesia Vol 16, No 3 (2009): December
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Fluorosis is a condition due to ingestion of excessive amounts of fluor which can cause the change in tooth structure and strength. However, there is still lack of explanation on the surface structure of crystal hydroxyapatite that influences the microscopic characteristic of fluorosis enamel. Objectives: To investigate the surface structure of crystal hydroxy-apatite in fluorosis enamel. Materials and Methods: Determination of fluor concentration and the surface structure of normal and fluorosis enamel specimen were carried out by using Scanning Electron Microscopy/Energy Disperse X-Ray (SEM/EDX). Results: Fluor concentration of fluorosis enamel was significantly higher with increased surface roughness and porosity than normal enamel. SEM observation also showed gaps areas between enamel rods and visible aprismatic zone in some regions. Conclusion: High level of fluor concentration on fluorosis enamel indicated the subtitution of OH- by F- increasing the surface roughness of enamel surface.DOI: 10.14693/jdi.v16i3.100

The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture

Journal of Dentistry Indonesia Vol 6, No 1 (1999): April
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Bisphosphonates are reported to have an inhibitory effect on bone resorption in vivo and in vitro. The present study examined the effect of bisphosphonate on the formation of osteoclast-like cells in vitro. When mouse bone marrow cells were cultured for 8 days with 108M 1α, 25-dihydroxyvitamin D3 (1α, 25(OH)2D3) numerous clusters of mononuclear and multinucleated cells formed, which stained positive for tartrate-resistant acid phosphatase (TRAP-positive). 1α, 25(OH)2D3 is known to stimulate osteoclast-like cell formation in a mouse bone marrow culture. Adding 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) inhibited the increased formation of osteoclast-like cells stimulated by this stimulant. A time-course experimental model showed that the number of osteoclast-like cells decreased slightly when drugs were given early in the culture period and decreased markedly when the drugs were given later or continuously in the culture period. These findings suggested that bisphosphonate had an effect on mature stage and significantly inhibit bone destruction by inhibit osteoclast-like cells formation. The amount of PGE2 production stimulated by 1 α, 25(OH)2D3 was dose depedently higher with HEBP and 3-amino-1-hydroxypropylidene-1, 1-bisphosphonate (APD). Showing that PGE2 production is high at the end of culture when the cells are going to undergo apoptosis. This showed in part, the known bone-resorbing activity of these agents.

Pharmacotherapy for Orofacial Pain

Journal of Dentistry Indonesia Vol 9, No 1 (2002): April
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

The sensation of pain is the means by which the body is made urgently aware of the presence of tissue damage. Pain represents a protective reflex for self-preservation. It is often pain that brings the patient to the dental office but also can be the factor that keeps the patient from seeking treatment at the appropriate time. Pain control is of great importance in dental practice. The clinician has to know the financial neuroanatomy, peripheral and central nervous system pathways, pain modulating system and various categories of pain of the head, neck and and face. Psychological and psychosocial factors also contribute to pain, whether pain arises endogenously from pathologic processes or exogenously from trauma or even dental treatment. The dentist’s task is threefold: first, to establish the correct diagnosis, second to find the cause of the pain, and third to select the treatment plan that addresses the patient’s complaint. By knowing the classification of orofacial pain, the clinician will easily make a diagnosis and determine the cause of the pain. After establishing the physical diagnosis it is easy then to determine the therapy to be made. The treatment in managing dental pain from pharmacotherapy is still effective using analgesic and local anasthetic drug.  

IL-1 & IL-6 in Bone: Local Translators in Cell-to-Cell Communications

Journal of Dentistry Indonesia Vol 6, No 3 (1999): December
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Interleukin-1 (IL-1) and interleukin-6 (IL-6) which are produced in bone marrow cells and bone cells play an important roles as local mediators between cells in the bone microenvironment. These interleukin of osteotropic cytokines have important regulatory roles in many organ processes, such as embryogenesis, angiogenesis, tumorigenesis, wound healing, and immune responses. The major targets of these cytokines in the bone is progenitor cells, and in particular, hematopoietic precursors of osteoclasts, are strongly influenced by these cytokines. Proliferation and differentiation of osteoblasts are modulated by IL-1 and IL-6 through autocrine and/or paracrine mechanisms. IL-1 and IL-6 both have the action on bone formation and resorption in normal and pathologic bone turnover. However, it seems almost impossible to clearly elucidate their roles. Further investigation are needed to give more information on the role of osteotropic cytokines in bone cell function that will advance bone research.