Retno Damajanti Soejoedono
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Native Virus Challenge Test Against Vaccinated Chickens With Commercial Active and Inactive IBD Vaccines

Media Veteriner Vol 5, No 4 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Vaccination trial were conducted on two groups of broiler day-old-chick (DOC) using active and inactive IBD vaccines. First Group was further divided into two groups: one received active IBD vaccination at 7 days of age, and the other received oral vaccination at 7 and 21 days of age, respectively. Both subgroups were then challenged against native IBD isolate, K-5, at 21 days of age. The second Group was arranged similar to the first group, but the second Group received inactive IBD vaccination subcutaneously, at 21 days of age. At 35 days of age, all chickens were challenged against native IBD isolate, K-5. The group which received active vaccine showed pathological change of the bursa, correlated to the bursa/body weight indices less than 0.70 (20 %) and the bursa lesion score (BLS) was 1.56. This pathological change was more obvious after second application of the vaccine. The group which received active and inactive vaccines revealed immune responses with mild lesion in the bursa. This im-munity could not protect the chickens after challenged with K-5 isolate, correlated to the bursa/body weight indices less than 0.70 (100 %) and BLS was 4.0. This results show that K-5 isolate belong to different subtype or variant.

Phenotypic and Serotypic Characterization of Staphylococcus aureus Strains from Subclinical Mastitis Cattle (KARAKTERISASI SECARA FENOTIPE DAN SEROTIPE STAPHYLOCOCCUS AUREUS YANG BERASAL DARI MASTITIS SUBKLINIK PADA SAPI)

Jurnal Veteriner Vol 17, No 1 (2016)
Publisher : Jurnal Veteriner

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Abstract

Staphylococcus aureus is known as a major causative agent of mastitis in dairy cattle. In the presentstudy, 104 isolates of Staphlylococcus originated from subclinical mastitis cattle characterized for thephenotypic properties and the presence of Staphylococcal protein A (Spa). Some bacteria were resistancesagainst several antibiotics were also studied, such as, streptomycin, cefepime, nitrofurantoin, amikacin,chloramphenicol, and ciprofloxacin. About 78% of the isolated were moderately sensitive to nitrofurantoin,while 89% were highly resistant to cefepime and ciprofloxacin. Using the various mammals’ sera, sevenisolates revealed the presence of Spa.

Phenotypic and Serotypic Characterization of Staphylococcus aureus Strains from Subclinical Mastitis Cattle (KARAKTERISASI SECARA FENOTIPE DAN SEROTIPE STAPHYLOCOCCUS AUREUS YANG BERASAL DARI MASTITIS SUBKLINIK PADA SAPI)

Jurnal Veteriner Vol 17, No 1 (2016)
Publisher : Jurnal Veteriner

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Abstract

Staphylococcus aureus is known as a major causative agent of mastitis in dairy cattle. In the presentstudy, 104 isolates of Staphylococcus originated from subclinical mastitis cattle characterized for thephenotypic properties and the presence of Staphylococcal protein A (Spa). Some bacteria were resistancesagainst several antibiotics were also studied, such as erythromycin, streptomycin, tetracycline, cefepime,nitrofurantoin, amikacin, chloramphenicol, and ciprofloxacin. About 78% of the isolated were moderatelysensitive to nitrofurantoin, while 89% were highly resistant to cefepime and ciprofloxacin. Using thevarious mammals’ sera, seven isolates out of 104 revealed the presence of Spa.

Deteksi Spesies Brucella pada Kambing di Rumah Potong Hewan Jakarta

Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected fromslaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and PolymeraseChain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limphand limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown.