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Effect of Various Concentration of Vegetable Protein in Hair Mask on The Hair Texture Helen, Ivony; Hadinoto, Idajani; Hadisoewignyo, Lannie; Soegianto, Lisa
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Effect of various concentrations of vegetable protein in hair mask on the hair texture including hair smoothness, hair shining, hair strength, and morphology of hair had been studied. Concentrations of vegetable protein are made 5%, 7.5%, and 10%. The hair mask was evaluated such as organoleptic, pH, viscosity, and allergy test. Hair mask used in six subjects that have damage hair for 8 times every 2 days. Then, the hair mask was evaluated such as hair smooth and hair shine that used tree trained panel and analyzed using non parametric method Q Cochran. Hair strength was evaluated using autograph  then  the  results  were    analyzes  using  ANOVA  test  (p<0.05),  while  hair morphology  was  evaluated  using  scanning  microscope  electron  (SEM).  The experimental  results  showed  that  vegetable  protein  in  hair  mask  increased  hair smoothness, hair strength, and morphology of damaged hair but did not give effect for hair shining. Various concentrations of vegetable protein (5%, 7.5%, and 10%) on hair mask given the same effect in hair texture (hair smoothness, hair strength, and hair morphology).
ANTIBACTERIAL AND ANTIBIOFILM POTENTIAL OF ETHANOLIC EXTRACT FROM BINTARO FLOWER (Cerbera odollam) AGAINST Staphylococcus aureus ATCC 6538 Lestari, Dwi Rahma Suci; Soegianto, Lisa; Hermanu, Liliek S.
UNEJ e-Proceeding 2016: Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UNEJ e-Proceeding

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Abstract

Staphylococcus aureus are examples of Grampositivebacteria that can cause infection.Treatment of infection with antibiotic synthesiscan bring its own problems, namely the resistanceof bacteria to antibiotics. Resistant bacteria is abacterial pathogen that is capable of formingbiofilms on living beings. Traditional medicine canbe another alternative in the treatment ofinfections. Natural compounds that have thepotential as an antibacterial commonly containsteroids, tannins, polyphenols, flavonoids1),alkaloids, saponins2).Some studies related to the use as an antibacterialBintaro explained that the mangrove plant thatbelongs to the family Apocynaceae is grown widelyin the southern coast of East Asia and the IndianOcean has antibacterial activity3).This study was conducted to determine theantibacterial activity and antibiofilm of ethanolicextract from Cerbera odollam flower againstStaphylococcus aureus.
EFFECTIVENESS OF BINTARO (Cerberra odollam Gaertn.) LEAF ETHANOLIC EXTRACT AGAINST Staphylococcus aureus IN-VITRO BIOFILM FORMATION Kanja, Filania S.; Soegianto, Lisa; Wijaya, Sumi
UNEJ e-Proceeding 2016: Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UNEJ e-Proceeding

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Abstract

Biofilm is a structural form of a group ofmicroorganisms protected by matrik extracellularcalled Extracellular Polymeric Substance (EPS),where EPS is a product produced by thesemicroorganisms itself and as a shelter from thebad influence in environment. The structure ofthese matrix is strings which cross each otherwhich can be adhesive for biofilm4).Biofilm from Staphylococcus aureus seen inchronicle infection cases as ulcer on diabeticsfoot, venous statis ulcer and pressure sores.Patients with infection wound with chronicleulcer in vein feet found positive culture ofStaphylococcus aureus as much as 88-93,3% froma number of infection found.Figure 1. Cerberra odollam Gaertn.Bintaro can be used as an analgesic,anticonvulsant, kardiotonik and antihypertensive2).The aim of this study is to test the effectivenessof the ethanol extracts of bintaro leaves (Cerberraodollam Gaertn.) towards the Staphylococcusaureus formation by in vitro.MATERIALSBintaro leaves (Cerberra odollam Gaertn.),Staphylococcus aureus culture ATCC 6538, petridish, ose wire , micro pipet, autoclave (AllAmerican Model No 25x, USA), incubator(Memmert and Binder, Germany), Laminar AirFlow (LAF) (Type V-130, Indonesia), microplate,microplate reader (Thermofisher Scientific,America), vacuum rotary evaporator (BÜCHI,Germany), weight analytic (Sartorius TE 214 S,Germany), oven (Memmert, Germany).
Perbandingan Antibakteri dari Ekstrak Etanol dan Fraksi Ekstrak Etanol Tanaman Ceguk (Quisqualis indica L.) Terhadap Staphylococcus aureus dan Escherichia coli Valeri, Sepvan; Soegianto, Lisa; Wijaya, Sumi
Jurnal Farmasi Sains dan Terapan Vol 2, No 2 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

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Abstract

Tanaman Ceguk merupakan tanaman yang berasal dari Myanmar dan Malaysia. Tanaman ini memiliki beberapa efek farmakologi antara lain immunomodulator, antihiperlipidemia, antipiretik, antioksidan dan antibakteri. Aktivitas antibakteri sebelumnya telah diteliti pada bagian bunga. Pada penelitian ini ekstrak etanol dan fraksi (n-heksana, etil asetat dan air) dari daun tanaman ceguk diuji aktivitas antibakterinya terhadap Staphylococcus aureus dan Escherichia coli. Fraksi aktif terpilih ditentukan dengan bioautografi kontak. Pengujian aktivitas antibakteri meliputi KHM (Kadar Hambat Minimum) dan KBM (Kadar Bunuh Minimum) dilakukan dengan metode mikrodilusi dan trifenil tetrazolium klorida 1% sebagai indikator visual. Golongan senyawa fraksi aktif ditentukan dengan uji kualitatif KLT dengan menggunakan penampak noda. Ekstrak dan fraksi n-heksana tanaman ini menghambat pertumbuhan pada bakteri Gram positif yaitu Staphylococcus aureus dengan nilai KHM 500 ppm pada fraksi n-heksana dan 1000 ppm untuk ekstrak. Ekstrak dan fraksi n-heksana tanaman ini tidak mampu membunuh Staphylococcus aureus dan Escherichia coli. Berdasarkan uji KLT, golongan senyawa fraksi aktif pada fraksi n-heksana yang diduga memiliki potensi sebagai antibakteri adalah golongan triterpenoid dengan harga Rf 0,63. Kata kunci : antibakteri, ceguk, fraksi, kadar hambat minimum, kadar bunuh minimum.
Uji Efek Sedasi dan Durasi Waktu Tidur Ekstrak Etanol Herba Putri Malu (Mimosa microphylla D.) pada Mencit (Mus musculus) Galur Swiss Webster Muliadi, Yeremia Kevin; Tamayanti, Wahyu Dewi; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan Vol 2, No 2 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

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Abstract

Sejak dahulu tanaman Putri Malu banyak digunakan oleh masyarakat sebagai obat batu ginjal, radang saluran nafas, anti mikroba, peluruh dahak, antipiretik, antiinflamasi, diuretik. Namun, tanaman Putri Malu dengan jenis spesies Mimosa microphylla D. masih jarang dilakukan penelitian serta masih sedikit literatur yang ada, terutama aktivitasnya dalam menimbulkan efek sedasi bila dibandingkan dengan tanaman Putri Malu dari spesies Mimosa pudica L. Oleh sebab itu dilakukan penelitian untuk menguji efek sedasi ekstrak herba Putri Malu (Mimosa microphylla D.) terhadap mencit (Mus musculus) galur Swiss webster untuk mengetahui potensi sedatifnya. Herba Putri Malu Mimosa microphylla D. diekstraksi dengan cara maserasi dengan etanol 96% dan diberikan kepada mencit jantan galur Swiss webster sebanyak 25 ekor yang dibagi dalam 5 kelompok dengan dosis pemberian 600 mg/kgBB, 1200 mg/kgBB, dan 2400 mg/kgBB masing-masing 1 ml/20 gBB. Penelitian ini dilakukan dengan berbagai metode: holeboard, evasion box, platform, rotarod untuk melakukan uji efek sedasi, uji induksi mula tidur, dan uji durasi waktu tidur, serta sebagai pembanding digunakan fenobarbital 30 mg/kgBB. Dari hasil percobaan uji sedatif, diketahui bahwa dosis 600 mg/kgBB ekstrak etanol herba Putri Malu (Mimosa microphylla D.) memiliki aktivitas sedatif terbaik dan pemberian pada 1200 mg/kgBB mampu memperpanjang durasi tidur mencit, namun ekstrak etanol herba Putri Malu (Mimosa microphylla D.) tidak mampu memperpendek waktu mula tidur mencit. Ekstrak etanol herba Putri Malu (Mimosa microphylla D.) memiliki aktivitas dalam menimbulkan efek sedatif, memperpanjang durasi waktu tidur, namun tidak mampu memperpendek waktu mula tidur mencit. Kata kunci: Mimosa microphylla D., efek sedatif, durasi waktu tidur, waktu mula tidur.
Pengaruh Pemberian Infus Kelopak Kering Rosella (Hibiscus sabdariffa) Terhadap Kadar Kolesterol Total Serum Darah Tikus Hiperkolesterolemia Octavia, Stefani Kartika; Surdijati, Siti; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan Vol 2, No 2 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

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Abstract

Rosella (Hibiscus sabdariffa) merupakan salah satu tanaman bermanfaat sebagai bahan makanan, minuman maupun bahan obat. Kelopak Rosella berkhasiat sebagai antihipertensi, antidiabetes, antikolesterol, terapi gangguan liver dan asam urat. Penelitian ini bertujuan untuk mengetahui apakah ada pengaruh yang signifikan dari pemberian infus kelopak kering Rosella dalam menurunkan kadar kolesterol total serum darah tikus hiperkolesterolemia. Penelitian ini menggunakan 25 tikus putih jantan galur Wistar yang diberi propiltiourasil 0,01% dan makanan tinggi kolesterol selama 2 minggu. Kemudian dibagi dalam lima kelompok, yaitu kontrol (PGA 3%), pembanding (Simvastatin 0,9 mg/kgBB) dan 3 kelompok uji yang diberi infus Rosella dosis 125 mg/kgBB, 250 mg/kgBB dan 500 mg/kgBB, secara oral selama 2 minggu. Data dianalisis dengan uji statistik Anova dilanjutkan uji Tukey HSD 5%. Perhitungan korelasi digunakan untuk mengetahui hubungan antara peningkatan dosis dengan penurunan kolesterol total. Tidak ada perbedaan bermakna pada setiap pasangan kelompok dengan signifikansi >0,05 untuk semua kelompok kecuali antara K1 dan K5 (signifikansi
EFFECT OF AFRICAN LEAF (VERNONIA AMYGDALINA) TO IL-6 AND IL-10 LEVEL ON STAPHYLOCOCCUC AUREUS INFECTION Setiawan, Lidwina Tri Kristanti; Nugraha, Jusak; Lestari, Pudji; Sinansari, Restry; Soegianto, Lisa; Tamayanti, Wahyu Dewi; Handayani, Luh Putu Trys Monika; Beatrix, Stephanie
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 4 (2019)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.96 KB) | DOI: 10.20473/ijtid.v7i4.9654

Abstract

Currently, infectious disease is increase in world wide. The African leaf (Vernonia amygdalina) – VA is used to antimicrobial treatment. It may protect the host against microbial attack in several ways. This plant has attracted the interest of researchers in recent decades because of the constituents have important roles in modulating immune system in bacteria infection. The aim of study is to analyze the prophylactic activity of VA’s ethanol extract in modulating the levels of IL-6 and IL-10 as well as the number of bacteria in male Wistar rats that were (Staphylococcus aureus) – SA – infected. There were as many as 30 rats were divided into 5 treatment groups: negative control (NC) was treated by CMC Na 2% (w/v); positive control (PC) was treated by 9mg/200g body weight (BW) of cephadroxil; T1; T2; and T3 were respectively treated with ethanol extract of VA of doses 20mg/200g BW; 40mg/200g BW and 80mg/200g BW. After the oral treatment was administered, all the rats were infected with 0.25mL (3x108cfu) SA via intra peritoneal route. Their blood was drawn in order to identify the IL-6 and IL-10 levels by ELISA. Furthermore, their peritoneal fluid was also taken to count the number of survived bacteria by pour plate method. The results are showed median of IL-6 and IL-10 levels as well as bacterial number respectively in NC 370.530pg/mL; 67.044pg/mL; 7.4x103cfu/mL; in PC 234.556pg/mL; 42.839pg/mL; 6.8x103cfu/mL,; in  T1 164.019pg/mL; 17.240pg/mL; 1.1x104cfu/mL; in T2 49.291pg/mL; 2.961 pg/mL; 6.3x103cfu/mL and in T3 43.342pg/mL; 13.235pg/mL; 7.1x103cfu/mL. These results are implied that VA’s ethanol extract is effective as a prophylactic agent to suppress the bacterial invasion at dose of 40mg/200g BW in Wistar rat particularly shown by the decrease level of IL-6 and the number of bacteria.
Isolasi dan Identifikasi Zat Antibakteri dan Antikuorum Sensing dalam Ekstrak Kelopak Bunga Rosela (Hibiscus sabdariffa L.) SOEGIANTO, LISA; HERTIANI, TRIANA; PRAMONO, SUWIJIYO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 2 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

This research was dried powder of Rosela calyx macerated with ethanol 70%-HCl (99:1) and then fractionated using n-hexane, ethyl acetate, n-butanol and water. All fraction was tested for antibacterial and antibiofilm activities by using the diffusion methods against S. aureus and E. coli. Quorum sensing inhibition was tested against Pseudomonas aeruginosa. Further fractionation was performed on the most active fraction by using VLC, followed by preparative TLC. Bioautography was done to guide the isolation of the active compound. Structure identification of the active compound was performed by analysing 1H-NMR, 13C-NMR, COSY, HMBC and LCMS data. The ethyl acetate fraction was the most active fraction against S. aureus showed by growth inhibition zone ,15.89 ± 0.37 mm (concentration of 10%) and 15.93 ± 0.72 mm (concentration of 20%), while against E . coli, 17.25 mm (concentration of 10%) and 17.35 mm (concentration of 20%). Formation inhibition of S. aureus biofilm observed at 62.05% ± 17.83 and 11.11% ± 23.13 in E.coli. Antiquorum sensing activity was observed as 19.23 ± 1.52 mm (concentration of 10%) and 20.89 ± 2.35 m at a concentration of 20%. Identification of the partial structure of the active compounds resulted a benzofuran derivative compounds.
Antidiarrheal Activity of The Combination of Ethanol Extracts of Turmeric Rhizomes, Gall oak Leaves, Guava Leaves and Meniran Herbs Wijaya, Sumi; Soegianto, Lisa
Jurnal Jamu Indonesia Vol 2 No 3 (2017): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (989.182 KB) | DOI: 10.29244/jji.v2i3.38

Abstract

Diarrheal disease is an endemic disease in Indonesia and also a potential disease that can cause death. In practice, the use of traditional medicines derived from plants still being considered by society due to its viability, economic factors and safety reasons. Several plants worked as antidiarrheal agents with the mechanism actions of (1) antibacterial (inhibited the growth of Escherichia coli, Shigella dysenteriae, Shigella flexneri, Staphylococcus aureus and Salmonella Typhi); (2) reduced the contraction of intestinal as results in the reduction of pain and the reduction of frequency of defecation or; (3) both mechanism of actions. Thus the purpose of the present study is to evaluate antidiarrheal activity of the combination of plant extracts which have been proved to have antidiarrheal activity.  Turmeric (Curcuma domestica) rhizomes, gall oak (Quercus lusitanica) leaves, guava (Psidium guajava) leaves and meniran (Phyllanthus niruri) herbs were used for this combination. The purpose of this combination was to minimize the doses (based on literature reviews turmeric at 5% and Meniran 10% inhibited the growth of Escherichia coli and Staphylococcus aureus, meanwhile guava leaves at 150 mg/kg BW and gall oak leaves at 10% reduced diarrhea in animal tested). Maceration was chosen for the extraction method using ethanol 96% as the solvent. Protection and transit intestinal method with loperamide HCl as comparison drug and castor oil for the induction of diarrhea were used for antidiarrheal activity, meanwhile diffusion and dilution methods using, inhibition zones, minimum inhibition concentration and minimum bactericidal concentration parameters were used for antibacterial activity. Tetracycline HCl with the concentration of 30 µg was used for the control. The results revealed that the combination of plant extract with the doses of 200 mg/KgBW (1:1:1:1) reduced diarrhea in mice with relaxation of smooth muscle and showed weak antibacterial effects on Eschericia coli and Staphylococcus aureus but strongly inhibited the growth of Salmonella Typhi.
Effect of Various Concentration of Vegetable Protein in Hair Mask on The Hair Texture Helen, Ivony; Hadinoto, Idajani; Hadisoewignyo, Lannie; Soegianto, Lisa
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (873.559 KB) | DOI: 10.14499/indonesianjcanchemoprev2iss1pp159-168

Abstract

Effect of various concentrations of vegetable protein in hair mask on the hair texture including hair smoothness, hair shining, hair strength, and morphology of hair had been studied. Concentrations of vegetable protein are made 5%, 7.5%, and 10%. The hair mask was evaluated such as organoleptic, pH, viscosity, and allergy test. Hair mask used in six subjects that have damage hair for 8 times every 2 days. Then, the hair mask was evaluated such as hair smooth and hair shine that used tree trained panel and analyzed using non parametric method Q Cochran. Hair strength was evaluated using autograph then the results were  analyzes using ANOVA test (p<0.05), while hair morphology was evaluated using scanning microscope electron (SEM). The experimental results showed that vegetable protein in hair mask increased hair smoothness, hair strength, and morphology of damaged hair but did not give effect for hair shining. Various concentrations of vegetable protein (5%, 7.5%, and 10%) on hair mask given the same effect in hair texture (hair smoothness, hair strength, and hair morphology).Keywords: vegetable protein, hair mask, hair texture