Lisa Soegianto
Fakultas Farmasi Unika Widya Mandala Surabaya
Articles
4
Documents
Effect of Various Concentration of Vegetable Protein in Hair Mask on The Hair Texture

Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Effect of various concentrations of vegetable protein in hair mask on the hair texture including hair smoothness, hair shining, hair strength, and morphology of hair had been studied. Concentrations of vegetable protein are made 5%, 7.5%, and 10%. The hair mask was evaluated such as organoleptic, pH, viscosity, and allergy test. Hair mask used in six subjects that have damage hair for 8 times every 2 days. Then, the hair mask was evaluated such as hair smooth and hair shine that used tree trained panel and analyzed using non parametric method Q Cochran. Hair strength was evaluated using autograph  then  the  results  were    analyzes  using  ANOVA  test  (p<0.05),  while  hair morphology  was  evaluated  using  scanning  microscope  electron  (SEM).  The experimental  results  showed  that  vegetable  protein  in  hair  mask  increased  hair smoothness, hair strength, and morphology of damaged hair but did not give effect for hair shining. Various concentrations of vegetable protein (5%, 7.5%, and 10%) on hair mask given the same effect in hair texture (hair smoothness, hair strength, and hair morphology).

ANTIBACTERIAL AND ANTIBIOFILM POTENTIAL OF ETHANOLIC EXTRACT FROM BINTARO FLOWER (Cerbera odollam) AGAINST Staphylococcus aureus ATCC 6538

UNEJ e-Proceeding 2016: Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UNEJ e-Proceeding

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Abstract

Staphylococcus aureus are examples of Grampositivebacteria that can cause infection.Treatment of infection with antibiotic synthesiscan bring its own problems, namely the resistanceof bacteria to antibiotics. Resistant bacteria is abacterial pathogen that is capable of formingbiofilms on living beings. Traditional medicine canbe another alternative in the treatment ofinfections. Natural compounds that have thepotential as an antibacterial commonly containsteroids, tannins, polyphenols, flavonoids1),alkaloids, saponins2).Some studies related to the use as an antibacterialBintaro explained that the mangrove plant thatbelongs to the family Apocynaceae is grown widelyin the southern coast of East Asia and the IndianOcean has antibacterial activity3).This study was conducted to determine theantibacterial activity and antibiofilm of ethanolicextract from Cerbera odollam flower againstStaphylococcus aureus.

EFFECTIVENESS OF BINTARO (Cerberra odollam Gaertn.) LEAF ETHANOLIC EXTRACT AGAINST Staphylococcus aureus IN-VITRO BIOFILM FORMATION

UNEJ e-Proceeding 2016: Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UNEJ e-Proceeding

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Abstract

Biofilm is a structural form of a group ofmicroorganisms protected by matrik extracellularcalled Extracellular Polymeric Substance (EPS),where EPS is a product produced by thesemicroorganisms itself and as a shelter from thebad influence in environment. The structure ofthese matrix is strings which cross each otherwhich can be adhesive for biofilm4).Biofilm from Staphylococcus aureus seen inchronicle infection cases as ulcer on diabeticsfoot, venous statis ulcer and pressure sores.Patients with infection wound with chronicleulcer in vein feet found positive culture ofStaphylococcus aureus as much as 88-93,3% froma number of infection found.Figure 1. Cerberra odollam Gaertn.Bintaro can be used as an analgesic,anticonvulsant, kardiotonik and antihypertensive2).The aim of this study is to test the effectivenessof the ethanol extracts of bintaro leaves (Cerberraodollam Gaertn.) towards the Staphylococcusaureus formation by in vitro.MATERIALSBintaro leaves (Cerberra odollam Gaertn.),Staphylococcus aureus culture ATCC 6538, petridish, ose wire , micro pipet, autoclave (AllAmerican Model No 25x, USA), incubator(Memmert and Binder, Germany), Laminar AirFlow (LAF) (Type V-130, Indonesia), microplate,microplate reader (Thermofisher Scientific,America), vacuum rotary evaporator (BÜCHI,Germany), weight analytic (Sartorius TE 214 S,Germany), oven (Memmert, Germany).

EFFECT OF AFRICAN LEAF (VERNONIA AMYGDALINA) TO IL-6 AND IL-10 LEVEL ON STAPHYLOCOCCUC AUREUS INFECTION

Indonesian Journal of Tropical and Infectious Disease Vol 7, No 4 (2019)
Publisher : Institute of Topical Disease

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Abstract

Currently, infectious disease is increase in world wide. The African leaf (Vernonia amygdalina) – VA is used to antimicrobial treatment. It may protect the host against microbial attack in several ways. This plant has attracted the interest of researchers in recent decades because of the constituents have important roles in modulating immune system in bacteria infection. The aim of study is to analyze the prophylactic activity of VA’s ethanol extract in modulating the levels of IL-6 and IL-10 as well as the number of bacteria in male Wistar rats that were (Staphylococcus aureus) – SA – infected. There were as many as 30 rats were divided into 5 treatment groups: negative control (NC) was treated by CMC Na 2% (w/v); positive control (PC) was treated by 9mg/200g body weight (BW) of cephadroxil; T1; T2; and T3 were respectively treated with ethanol extract of VA of doses 20mg/200g BW; 40mg/200g BW and 80mg/200g BW. After the oral treatment was administered, all the rats were infected with 0.25mL (3x108cfu) SA via intra peritoneal route. Their blood was drawn in order to identify the IL-6 and IL-10 levels by ELISA. Furthermore, their peritoneal fluid was also taken to count the number of survived bacteria by pour plate method. The results are showed median of IL-6 and IL-10 levels as well as bacterial number respectively in NC 370.530pg/mL; 67.044pg/mL; 7.4x103cfu/mL; in PC 234.556pg/mL; 42.839pg/mL; 6.8x103cfu/mL,; in  T1 164.019pg/mL; 17.240pg/mL; 1.1x104cfu/mL; in T2 49.291pg/mL; 2.961 pg/mL; 6.3x103cfu/mL and in T3 43.342pg/mL; 13.235pg/mL; 7.1x103cfu/mL. These results are implied that VA’s ethanol extract is effective as a prophylactic agent to suppress the bacterial invasion at dose of 40mg/200g BW in Wistar rat particularly shown by the decrease level of IL-6 and the number of bacteria.