Hardyanto Soebono
Bagian Kulit dan Kelamin, Fakultas Kedokteran UGM, Yogyakarta

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PENGEMBANGAN DIAGNOSIS TUBERKULOSIS PADA HEWAN KESAYANGAN ANJING MENGGUNAKAN ANTIGEN SPESIFIK Mycobacterium tuberculosisESAT-6 DAN CFP-10 Tjahajati, Ida; Asmara, Widya; Soebono, Hardyanto
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Early diagnosis is one of the important methods to  control tuberculosis because this disease is zoonotic which easily spread through the air. Early detection of tuberculosis in dog is also very important since dog as petanimal have a potency to transfer the disease to human or other animals. The discovery of two specific M.tuberculosisantigens, ESAT06 and CFP-10, provide the opportunity to developa specific diagnostic kit for tuberculosis by using ELISA based on the secretion of IFN-γ. The development of a tuberculosis diagnostic kit based on this molecular biology and immunological method would provide a good alternative method to detect tuberculosis specially, accurately as early as possible. The result of this experiment would provide contribution for the development of health science and technology, especially in the eradication of tuberculosis. Keywords: diagnosis, tuberculosis, ESAT-6, CFP-10, interferon-gamma.
Faktor risiko kejadian kusta Aprizal, Aprizal; Lazuardi, Lutfan; Soebono, Hardyanto
Berita Kedokteran Masyarakat (BKM) Vol 33, No 9 (2017)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.5 KB) | DOI: 10.22146/bkm.25569

Abstract

Latar Belakang : Kabupaten Lamongan merupakan daerah endemis kusta di Provinsi Jawa Timur dengan angka prevalensi tahun 2007 – 2011 lebih dari 1/10.000 penduduk. Sebagian besar penderita kusta tipe Multibasiler (MB) (71%). Tujuan : Untuk mengetahui faktor risiko kejadian kusta di Kabupaten Lamongan Provinsi Jawa Timur antara lain ; status ekonomi, vaksinasi BCG, kondisi rumah (kondisi lantai rumah, kepadatan hunian dan air bersih) dan perilaku individu (kontak penderita, menggunakan alas kaki dan mandi menggunakan sabun mandi). Metode : Metode  penelitian kasus kontrol.  Subjek dalam penelitian ini adalah penderita kusta yang tercatat di register puskesmas, sedangkan kontrol adalah penderita bukan kusta. Sampel berjumlah 170 terdiri dari 85 kasus dan 85 kontrol. Analisis data bivariat menggunakan uji Kai-kwadrat dan analisis multivariat menggunakan uji Regresi Logistik, besar risiko menggunakan Odds Ratio (OR). Hasil : Faktor-kator risiko yang berhubungan dengan kejadian kusta di Kabupaten  Lamongan antara lain ; status ekonomi atau pendapatan keluarga (OR=4,3 dan P=0,001), vaksinasi BCG (OR=4,3 dan P=0,050), kepadatan hunian (OR=3,2 dan P=0,001), kondisi lantai rumah (OR=2,8 dan P=0,051) dan sumber air bersih (OR=2,1 dan P=0,033), riwayat kontak (OR=7,8 dan P=0,001), kebiasaan mandi menggunakan sabun mandi (OR=3,1 dan P=0,022) dan penggunaan alas kaki (OR=3,1 dan P=0,004). Sedangkan faktor risiko yang dominan vaksinasi BCG (OR = 8,1 dan P=0,025). Kesimpulan : Terdapat hubungan antara status ekonomi atau pendapatan keluarga,vaksinasi BCG, kondisi rumah (kondisi lantai rumah, kepadatan hunian dan air bersih) dan perilaku individu (kontak penderita, menggunakan alas kaki dan mandi menggunakan sabun mandi) dengan kejadian kusta di Kabupaten Lamongan. Faktor risiko yang paling dominan adalah vaksinasi BCG.
Effect of Oxidative Stress on AhpC Activity and Virulence in katG Ser315 Thr Mycobacterium tuberculosis Mutant Rintiswati, Ning; Wibawa, Tri; Asmara, Widya; Soebono, Hardyanto
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

AbstractMycobacterium tuberculosis strains resistance to INH is mainly caused by the alteration in several genesencoding the molecular targets. Mutation of katG at codon 315 especially Ser315Thr are responsible forINH resistance in a large proportion of TB cases. The aim of this study is to evaluate the influence of stressoxidative on AhpC activity of katG Ser315Thr of M.tuberculosis, and to find out the relation of AhpC and thevirulence of this mutant. The study design was laboratoric experimental, subjects of study were M.tuberculosisINH resistance strains, and the treatment were serial dose of H2O2. Eighty five M.tuberculosis INH resistantclinical strain were screened for mutation of katGSer315Thr by PCR/RFLP and characterized on the basis ofphenotypic properties (catalase activity and AhpC activity). AhpC activity of katG Ser315Thr M.tuberculosisstrains in response to oxidative stress condition was evaluated by culturing the strains on liquid culturemedium containing 1mM H2O2. To ascertain role of AhpC in the virulence of katGSer315Thr mutant strains, themutants were infected into human macrophages culture, and several indicator of virulence were observed (i.e:replication competence, and apoptosis induction on human macrophages). The results showed that katG Ser315Thr were identified in 23 (27,05%) of 85 INH resistance strains, all mutant strains had decrease of catalaseactivity. AhpC activity of katG Ser315Thr of M.tuberculosis increased significantly with increase of hydrogenperoxide dose. In addition , it has been shown that increased AhpC activity related to replication ability ofmutant, and reduction of apoptosis macrophages induction significantly. We conclude that the productionof AhpC of katG Ser315Thr M.tuberculosis induced by oxidative stress. There was a role of AhpC in virulenceof the M.tuberculosis katG Ser315Thr strains by replication capability and macrophages apoptosis.Keywords : katG Ser315Thr Mycobacterium tuberculosis- oxidative stress - AhpC - virulence
Faktor risiko dan pola distribusi kusta di Yogyakarta Efrizal, Efrizal; Lazuardi, Lutfan; Soebono, Hardyanto
Berita Kedokteran Masyarakat (BKM) Vol 32, No 10 (2016)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.367 KB) | DOI: 10.22146/bkm.12345

Abstract

Risk factors and distribution of leprosy in YogyakartaPurposeThe purpose of this paper was to identify risk factors and distribution pattern of leprosy in Yogyakarta. MethodsA case-control study was conducted involving 112 samples which consisted of 56 cases and 56 controls. ResultsThe study found three risk factors of leprosy: BCG vaccination, humidity and lighting. BCG vaccination was the most dominant risk factor of leprosy. The study also showed that the distribution pattern of leprosy in Yogyakarta was clustered. BCG vaccination was the most dominant risk factor for leprosy occurrence. Distribution of leprosy events were wide-spread and dispersed in the city of Yogyakarta, Sleman, Bantul and Kulon Progo, and only in Gunung Kidul Regency were clustered, and overall in Yogyakarta Special Region was clustered. The existence of cases of leprosy was largely distant with primary health care, away from roads and rivers as well as more dominant in settlements in mountainous areas.ConclusionActive case finding of leprosy by health workers, especially in clustered areas to decide the transmission of leprosy either from humans or the environment, with training of leprosy and counseling officers to remove leprosy-related stigma needs to be done. These efforts need to be coordinated between the Health Office and community health centers so that the number of cases in each region can be known.
Effect of Oxidative Stress on AhpC Activity and Virulence in katG Ser315 Thr Mycobacterium tuberculosis Mutant Rintiswati, Ning; Wibawa, Tri; Asmara, Widya; Soebono, Hardyanto
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.371 KB) | DOI: 10.22146/ijbiotech.16369

Abstract

Mycobacterium tuberculosis strains resistance to INH is mainly caused by the alteration in several genesencoding the molecular targets. Mutation of katG at codon 315 especially Ser315Thr are responsible forINH resistance in a large proportion of TB cases. The aim of this study is to evaluate the influence of stressoxidative on AhpC activity of katG Ser315Thr of M.tuberculosis, and to find out the relation of AhpC and thevirulence of this mutant. The study design was laboratoric experimental, subjects of study were M.tuberculosisINH resistance strains, and the treatment were serial dose of H2O2. Eighty five M.tuberculosis INH resistantclinical strain were screened for mutation of katGSer315Thr by PCR/RFLP and characterized on the basis ofphenotypic properties (catalase activity and AhpC activity). AhpC activity of katG Ser315Thr M.tuberculosisstrains in response to oxidative stress condition was evaluated by culturing the strains on liquid culturemedium containing 1mM H2O2.To ascertain role of AhpC in the virulence of katGSer315Thr mutant strains, themutants were infected into human macrophages culture, and several indicator of virulence were observed (i.e:replication competence, and apoptosis induction on human macrophages). The results showed that katG Ser315Thr were identified in 23 (27,05%) of 85 INH resistance strains, all mutant strains had decrease of catalaseactivity. AhpC activity of katG Ser315Thr of M.tuberculosis increased significantly with increase of hydrogenperoxide dose. In addition , it has been shown that increased AhpC activity related to replication ability ofmutant, and reduction of apoptosis macrophages induction significantly. We conclude that the productionof AhpC of katG Ser315Thr M.tuberculosis induced by oxidative stress. There was a role of AhpC in virulenceof the M.tuberculosis katG Ser315Thr strains by replication capability and macrophages apoptosis.
Association between HLA-B alleles and nevirapine-induced allergies among Indonesian HIV patients Pudjiati, Angela Satiti Retno; Oktarina, Dyah Ayu Mira; Soebono, Hardyanto; Sauda, Saihas; Paramita, Dewi Kartikawati; Dwiprahasto, Iwan; Djoerban, Zubairi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.093 KB) | DOI: 10.19106/JMedSci004804201604

Abstract

This study aimed to investigate the association between human leukocyte antigen-B(HLA-B) alleles and nevirapine allergy in HIV patients in Indonesia. A case control studywas conducted involving 147 HIV patients comprising of 50 patients with and 97patients without nevirapine allergy as control. The HLA-B allele typing was conducted byusing polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP),followed by sequencing. Bivariate analysis using Chi-square (X2) test and multivariatelogistic regression with significance level at p<0.05 were applied to analysis the data.Among 147 subjects, 34 with HLA-B alleles were identified. Bivariable analysis showedthat HLA-B*58 allele was the most significant risk factor for the nevirapine allergy (OR= 3.67; 95% CI: 1.79 to 7.54), while HLA-B*35 allele was a protective factor (OR =0.18; 95% CI: 0.08 to 0.42). Multivariate logistic regression analysis showed that youngmen and HLA-B*58 allele were the significant risk factors of nevirapine allergy (OR: 4.63;95% CI: 2.02 to 10.61), while older women with the HLA-B*35 was able to reducethe risk of nevirapine allergy approximately 81% (OR: 0.19; 95% CI: 0.08 to 0.49). Inconclusion, young male with the HLA-B*58 allele are the high risk factor for nevirapineallergy in Indonesian HIV patients.
Association between atopy and allergic contact dermatitis in Dr. Sardjito General Hospital Yogyakarta Fitria, .; Danarti, Retno; Soebono, Hardyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 02 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.829 KB) | DOI: 10.19106/JMedScie004502201305

Abstract

Association between atopy and development of allergic contact dermatitis (ACD) remains controversial. T cell disfunctions in a patient with atopy complicate the process of nickel sensitization. On the other, the decrease of the skin barrier function and overexpression of Langerhans cells in the patient facilitate the sensitization.  This study aimed to evaluate the association between atopy and incidence of nickel ACD. A case-control study was carried out in Allergic and Immunology Sub Department of Dermato-Venereology Policlinic, Dr. Sardjito General Hospital, Yogyakarta, involving 54 nickel ACD patients as case group and 74 healthy subjects as control group. All subjects underwent prick test allergens i.e. house dust, dust mite, cockroach, mixed fungi, nuts and egg white. The skin reaction was considered as a positive result if a wheal diameter of at least 3 mm larger than the negative control or a minimum of half of the positive control. The relationship between atopy and the nickel ACD incidence was analyzed using Chi-Square test with confidence interval (CI) of 95%. A significant association between atopy and the nickel ACD incidence was observed in this study. Subjects with atopy to  ≥1 allergen had risk of nickel ACD 3.74 higher than subjects without atopy (odds ratio/OR=3.74; 95%CI = 1.64-8.53).  Furtheremore, subjects with atopy to  ≥2 allergens had risk of nickel ACD 3.74 higher than subjects without atopy (OR=2.08; 95%CI = 1.01-4.29). In conclusion, atopy is a risk factor of nickel ACD.
Association between environmental allergen sensitization with severity of atopic dermatitis in children and young adult at Dr. Sardjito General Hospital, Yogyakarta Brahmanti, Herwinda; Trisnowati, Niken; Danarti, Retno; Soebono, Hardyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 01 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The important role of aeroallergens and food allergens as the most common environmental allergens in exacerbationof atopic dermatitis (AD) iswell known. Sensitization is an essential processwhich correlateswith clinicalmanifestationof AD. The study of AD in Indonesia, especially in Yogyakarta, has not been reported, yet. The aim of study is toevaluate the association between sensitization of environmental allergens with clinical severity of AD in children andyoung adult at Dr. Sardjito General Hospital, Yogyakarta. An analytic observational cross sectional study wasperformed on 33 children and young adult with AD. The severity of disease was determined by SCORing AtopicDermatitis (SCORAD). Assessment of sensitization was performed using specific IgE serum, atopy patch test, andprick test. Data were analyzed using chi-square or Fisher exact test and prevalence ratio (PR) with significancevalue of p<0.05 and 95%confidence interval (CI). The results showed that specific IgE positivity was associatedwith severity of AD. Percentage of specific IgE positivity to house dust mite was significantly higher in subject withmoderate/severe AD compared to those of mild AD (p=0.049; PR 1.13; 95% CI 1.01-1.59). The result was alsosimilar for cat dander (p=0.041; PR 1.1; 95%CI 1.09-4.98), cow’s milk (p=0.038; PR 1.21; 95% CI 1.02-2.2),and egg white (p=0.027; PR 1.23; 95% CI 1.15-2.97). Whereas specific IgE positivity to fish allergen was notstatistically different in subject with moderate/severe AD compared to those with mild AD (p=0.061; PR 0.8; 95%CI 0.76-2.8). According to atopy patch test and prick test result, no association was found between allergensensitization and severity of AD. If allmethodswere combined to increase the sensitivity of sensitizationmeasurement,then the association was found for all allergens. It could be concluded that environmental allergens sensitization isassociated with severity of AD in children and young adult at Dr. Sardjito General Hospital, Yogyakarta.Key words: atopic dermatitis-disease severity-sensitization-aeroallergen-food allergen
The effect of mitomycin-c in keloid fibroblast cultures Dachlan, Ishandono; Aryandono, Teguh; Wahyuningsih, Mae Sri Hartati; Soebono, Hardyanto; Wirohadidjojo, Yohanes Widodo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 3 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.667 KB) | DOI: 10.19106/JMedSci004803201605

Abstract

ABSTRACTKeloid occurs due to hyperactivity of keloid fibroblast (KF) in proliferation, migration, collagen deposition, together with low rates of collagen degradation. These are under the responsibility of TGF-b. Mitomycin C (MC) is used for treating keloid by a topical application during surgery at the level of 0.02% to 0.08%. Unfortunately, the lowest effective level of MC for keloid has not been determined yet. We aimed to determine the lowest effective level of MC in the suppression of KF activities. Various levels of MC diluted in growth medium were administered on KF that were isolated from six patients. After 24 hours and 72 hours of incubation, cellular proliferation, collagen deposition, cellular migration and level of TGF-b, were analyzed. Application of 120 uM MC on KF culture for 24 hours could significantly reduce TGF-b production from 1265.74 ± 274.81 pg/mL to 265.17 ± 12.20 pg/mL; proliferation index from 100% to 84.01 ± 12.91%; inhibit cellular migration to 64.38 ± 3.66%; but reduce collagen depositions from 100% to only 91.13 ± 10.19%. The lowest MC level is on 30 uM or equal with 0.001%. In conclusion, the lowest level of MC can suppress the activities of KF is 0.001%. Moreover, due to low activity in inhibiting collagen deposition, MC would be better as an adjuvant drug for keloid surgery.
Lepromatous leprosy mimicking systemic lupus erithematosus: a case report Kusumaningrum, Niken; Purnamawati, Schandra; Winarni, Dwi Retno Adi; Soebono, Hardyanto
Medical Journal of Indonesia Vol 28, No 1 (2019): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7167.123 KB) | DOI: 10.13181/mji.v28i1.1793

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The clinical manifestations of leprosy are highly variable, and the disease is notorious for being “a great imitator” of several other conditions. Leprosy may manifest with a variety of phenomena resembling those of autoimmune diseases. Herein, we report a 33-year-old male presenting with wounds on his left leg and hyperpigmented skin lesions all over his body. Six years earlier, the patient was diagnosed with systemic lupus erythematosus (SLE). However, therapy for SLE did not control his symptoms; instead, the patient developed features of leprosy, such as anesthetic skin lesions, nerve enlargement, and tenderness. Tests for antinuclear antibodies and anti-double stranded DNA antibodies were negative. Slit-skin smear showed a bacterial index of 6+ and morphological index of 10 %. Lupus band test results were negative. Histological findings were compatible with lepromatous leprosy. The clinical and serological similarities between leprosy and SLE may lead to erroneous diagnosis. Thus, clinicians should be aware of this characteristic for correct diagnosis.