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Karakter Fisik dan Kimia Buah Pepaya pada Stadia Kematangan Berbeda Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of the experiment was to investigate the physical and chemical characteristics of three stadia of maturity based on a range of peel color from green to yellow or based on percentage of the yellow area of fruit peel (stadium 1 = 25-49 % yellow, stadium 2 = 50-74 % yellow, and stadium 3 = above 75 % yellow) on six genotypes of papaya. Each genotype exhibited different days to maturity for each stadium. The fruits of stadium 1, 2 and 3 for IPB 1 were picked at 130, 135, and 140 days after anthesis (DAA); IPB 10A at 160, 165, and 170 DAA;  IPB 1 x PB 174 at 135, 140 and 145 DAA; while PB 174,  IPB 1 x IPB 10A and IPB 10A x PB 174 were picked at 140, 145 and 150 DAA, respectively. The results indicated that peel firmness was affected by maturity stage on female fruit of IPB 10A. Maturity stage affected chemical characteristics of papaya included total soluble solids (TSS) content (IPB 10A, female fruit of  PB 174, female fruit of IPB 1 x IPB 10A, and female fruit of IPB 1 x PB 174), vitamin C content (hermaphrodite fruit of 10 A, female fruit of IPB 1 x IPB 10A) and juice pH (hermaphrodite fruit of  IPB 1).  IPB 1 genotype can be harvested at all stadia of maturity stage. Hermaphrodite and female fruit of IPB 10 A, female fruit of  PB 174, female fruit of  IPB 1 x IPB 10A and female fruit of IPB 1 x  PB 174 genotype would be better harvested at stadium 3 of maturity stage.   Keywords: Carica papaya, papaya genotype, hermaphrodite fruit, female fruit, fruit quality, fruit maturity stage
Analisis Marka RAPD yang Te rpaut dengan To leransi terhadap Naungan pada Kedelai Wirnas, Desta; Sopandie, Didy; Trikoesoemaningtyas, ,; Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of the research was to identify RAPD markers linked to QTL related to agronomic traits of soybean under low-light intensity condition.  The genetic material used in the QTL analysis based on RAPD Markers were  Ceneng, Godek, and  F6 RILs derived from hybridization between  Ceneng (tolerant parent) and Godek (sensitve parent). The results of molecular analysis showed that 9 primers were polymorphic and linked to the tolerant parent.  Primers produce 14 RAPD markers which were polymorphic and linked to the tolerant parent. The markers were distributed into a linkage group that containing seven markers. RAPD Markers (OPE15-800, OPM20-800) were linked to two QTL controlling number of productive node and seed weight, respectively.  The marker linked to the tolerant parent could be used as a marker assisted selection for high-yielding soybean lines under low-light intensity.    
Karakter Morfo-Fisiologi Daun, Penciri Adaptasi Kedelai terhadap Intensitas Cahaya Rendah Kisman, ,; Khumaida, Nurul; Trikoesoemaningtyas, ,; Sobir, ,; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The main objective of this study was to identify the leaf morpho-physiological characters as markers for adaptation of soybean to low light intensity. The field experiment was conducted using split plot design with 3 replications. First factor was soybean genotype consisted of shade tolerant genotypes (Ceneng, Pangrango) and sensitive genotypes (Godek, Slamet). Second factor was low light intensity consisted of: L0 = control (under full sun light), L1 = 5 days under 50% shading (exposed after 21 days after planting/DAP), L2 = 5 days under dark condition (exposed after 21 DAP), L3 = 3 days 50% shading + 5 days sun light (exposed after 18 DAP), dan L4 = 3 days 50% shading + 3 days sun light + 5 day dark conditions (exposed after 15 DAP). The 50% shading was made artificially using black plastic paranet with 50% light transmitted. Results of this study showed that under the conditions of low light intensity, leaf morpho-physiological characters of soybean that could be used as markers for adaptation were leaf area, specific leaf weight, and chlorophyll content. Leaves of shade tolerant genotypes were wider and thinner and also contained higher chlorophyll especially for chlorophyll b than that of shade sensitive genotypes under low light intensity.   Key words :  Low light intensity, tolerant genotype, leaf area, chlorophyll, specific leaf weight
Character, Xanthone Content and Antioxidant Properties of Mangosteen Fruit’s Hull (Garcinia mangostana L.) at Several Fruit Growth Stadia Kurniawati, Ani; Poerwanto, Roedhy; Sobir, ,; Effendi, Darda; Cahyana, Herry
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 3 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this study was to evaluate the characteristics of mangosteen fruit’s hull, xanthone content, and antioxidant potential on various stadia of mangosteen fruit development. The experiment  was conducted in September 2006 until July  2008 using randomized complete block design, with three replications at maturation stage i.e. 1, 2, 3, and 4 months after anthesis. The parameters being observed were fruit’s hull characters, xanthone content, and antioxidant capacity.  The results of this study showed that fruit diameter and fruit weight increased until three months after anthesis and then did not change significantly once they entered the process of maturity. Thickness of fruit’s hull differed significantly among maturation stage. The thickness of fruit hull was observed at two months and the highest weight was at three months after anthesis. Xanthone content of mangosteen fruit’s hull at a month up to four months after anthesis did not differ significantly however capacity of antioxidant differed significantly among fruit ages, the IC50 values increased with the increase of fruit maturation.   Keywords: Antioxidant, fruit growth, fruit’s hull, mangosteen, xanthone
Rekomendasi Pemupukan Kalium untuk Tanaman Nenas Berdasarkan Status Hara Tanah Safuan, La Ode; Poerwanto, Roedhy; Susila, Anas Dinurrohman; Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 1 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Potassium (K) is required in a large amount for plant growth and production of most fruit crops, including pineapple. However, excess application may decrease its growth and production. Therefore, fertilizer application must be site specific, based on soil nutrient status and plant requirements. The aims of the research were (1) to determine the soil K nutritional status of pineapple and (2) to determine the optimum dosage of  K fertilization for pineapple grown in Sawah Baru, Darmaga, Bogor.  The research was conducted using split plot randomized block design with five soil K status as main plots i.e. 0, 70, 140, 210, and 280 kg K2O ha-1 representing status levels of very low, low, moderate, high, and very high, respectively. The sub plot was dosage of  K fertilizer i.e. 0, 200, 400, 600, and 800 kg K2O ha-1. The result of the research showed   that plant growth and production of pineapple were improved by soil K nutrient status and dosage of K application.  The  level  of  soil K  nutrient  availability  was  classified  into  low  (50 ppm K2O). Potassium fertilizer is recommended for the soil with low  soil K status of 634 kg K2O ha-1.    Keywords: Ananas comosus, fertilization, potassium, soil nutrient status
Pengaruh Batang Bawah dan Jenis Tunas pada Mikrografting Manggis (Garcinia mangostana) secara In Vitro Handayani, Rd. Selvy; Poerwanto, Roedhy; Sobir, ,; Purwito, Agus; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 1 (2013): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The aim of this study was to investigate the effect of rootstock and shoot types on in vitro mangosteen micrografting.The experiment was arranged in completely randomized design (CRD) with two factors. The first factor was the rootstocktype, i.e. rooted planlet from the germination of quartered seed, and rooted planlet from the germination of undivided seeds.The second factor was the developmental phase of scion, i.e. dormant buds, and flush (had new leaf more than 2-4 mm). Theresults showed that rootstock derived from the germination of undivided seed had a higher success rate than other treatmentson all variables, except for number of new leaves. The use of flush as scion was better than dormant buds; flush resulted in ahigher percentage of successful micrograft and longer shoots. In vitro micrografting had a better growth rate than grafting at the same age. The results of anatomical observation conducted at four months after micrografting demonstrated that there was a good graft union, indicated by excellent fusion between rootstock and scion xylem tissues.Keywords: flush, in vitro, micrografting, rootstock, scion
IDENTIFICATION OF CO-DOMINANT DNA MARKER TIGHTLY LINKED TO Tm-2 LOCUS IN TOMATO Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 27, No 3 (1999): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

Tm-2 adalah gen yang mengendalikan ketahanan terhadap tomato mosaic virus (ToMV) pada tomat (Lycopersicon esculentum) dan berasal dari Lycopersicon peruvianum. Pada penelitian ini digunakan dua galur tomat yang mempunyai latar belakang genetik yang sarna GCR26 dan GCR3 6, akan tetapi keduanya dapat dibedakan oleh adanya gen Tm-2 pada GCR36. Melalui analisis random amplified-polymorphic DNA (RAPD) terhadap dua galur tomat tersebut di atas diketahui adanya primer RAPD yang menghasilkan fragmen spesifik untuk GCR236. Selanjutnya fragmen tersebut sekuennya dianalisis, dan dari hasil analisis sekuen tersebut dibuat sepasang primer sequence characterized amplified region (SCAR) Yang merupakan marka DNA kodominan yang bisa membedakan baik genotipe homozigot gen Tm-2. Oleh karena fragmen dihasilkan terkait dengan Tm-2, maka primer SCAR tersebut merupakan sarana yang dapat digunakan untuk pemulian tanarnan tomat bagi pembentukan varietas tahan virus.
Analisis Kedekatan Hubungan antar Genotipe Pepaya Berdasarkan Karakter Morfologi dan Buah Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso D.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> A study was conducted to determine the variation and relationships among  papaya genotypes based on morphological and fruit characteristics  in order to produce  high quality papaya fruits. Fruit characterization study is very useful for genotype improvement and genotype classifi  cation of papaya. In this study the morphological characters of 36 genotypes were analyzed to determine their phenotypic variabilities.  The relationships between genotypes based on all of the morphological and fruit characteristics were tested by subjecting the data to multivariate principal component analysis and to cluster analysis. Based on the dendrogram generated from vegetative and generative characters, the 36 genotypes could be grouped into 11 clusters on a threshold of 1.6 and formed 6 clusters on a threshold of 1.8.  The dendrogram was able to explain the close relationship between IPB 5 x IPB 1 and IPB 5 x IPB 4, IPB 2 and IPB 7, IPB 1 and IPB 3 genotype. The scattered diagram of generative variable divided the papaya genotypes into three groups based on fruit sizes i.e small group (IPB 1, IPB 3, IPB 4, IPB 3 x IPB 4, IPB 1 x IPB 9), medium group (IPB 5, IPB 7, IPB 8, IPB 9) and big group (IPB 2, IPB 10).  The IPB 1, IPB 3 and IPB 4 were different from IPB 2 in fruit shapes, petal length of male fl  owers, infl orescence size and fruit length. The hybrid plants obtained from crossings with IPB 10 were different from the other genotypes in the colours of female-, hermaphrodite-, and male fl  ower-lobes. Subsequently the scatter diagrams also revealed that several genotypes i.e. IPB 2 x IPB 6, IPB 1 x IPB 5,  IPB 1 x IPB 9, IPB 5 x IPB 1 and IPB 5 x IPB 2 had superior characters  ideotype similar to IPB 1, IPB 3 and IPB 8 genotypes. Keywords: Carica papaya, hermaphrodite, female, dendrogram, scatter diagram, ideotype
Pola Pewarisan Adaptasi Kedelai (Glycine max L. Merrill) terhadap Cekaman Naungan Berdasarkan Karakter Morfo-Fisiologi Daun Kisman, ,; Trikoesoemaningtyas, ,; Sobir, ,; Khumaida, Nurul; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this study was to analyze the inheritance pattern of soybean adaptation to shade stress based on leaf morpho-physiological characters. Genetic materials used in this study consisted of 22 plants of low irradiance (LI)-tolerant genotype (Ceneng) and 22 plants of LI-sensitive genotype (Godek); 21 plants of F1 (Ceneng x Godek); and 114 plants of F2 populations (derived from F1). These populations were planted under shading of paranet 50%. The population was arranged in a Randomized Complete Block Design with two replicates. Analysis of inheritance of soybean adaptation involved estimation of heritability (broad sense) and gene action. Results of this study showed that: adaptation of soybean to low light stress based on characters of yield per plant was highly heritable (68% of broad sense) with partial dominant mode of action. Characters of leaf area and specific leaf weight were highly (68% of broad sense) and moderately (48% of broad sense) heritable, respectively, with additive mode of action. Soybean adaptation based on leaf physiological characters (chlorophyll contents) was highly heritable (70% - 86% of broad sense) in epistatic mode of action.   Key words:  Soybean, leaf morpho-physiological character, heritability, gene action
Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 Lizawati, ,; Poerwanto, Roedhy; Sobir, ,; Rusmana, Iman; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation