WELLYZAR SJAMSURIDZAL
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424

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Molecular Identification and Diversity of Yeasts Associated with Apis cerana Foraging on Flowers of Jatropha integerrima BASUKRIADI, ADI; SJAMSURIDZAL, WELLYZAR; PUTRA, BANGGA BERISTAMA
Microbiology Indonesia Vol 4, No 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

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There are only a few reports from tropical countries, and none from Indonesia, on yeasts associated with the Asiatic honeybee, Apis cerana. Here we report on yeasts associated with A. cerana foraging on flowers of Jatropha integerrima in the campus of the Universitas Indonesia, Depok, Indonesia. Yeasts were isolated from guts of 30 individual pollen-collecting bees (PCB) and nectar-collecting bees (NCB), and identified by their internal transcribed spacer (ITS) regions of their rDNA sequences. Based on ITS regions sequence data, 14 representative yeast isolates obtained from A. cerana were found to be closely related to Aureobasidium pullulans, Dothioraceae sp., Candida cf. apicola, C. cf. azyma, C. cellae, Metschnikowia sp., Kodamaea ohmeri and Yarrowia lipolytica. Undescribed yeast of the genus of Metschnikowia was also discovered in this study. At present, we assume there is association between C. cf. apicola and species closely related to C. cellae with A. cerana. Yeasts species associated with PCB differ from those found in NCB, indicating that PCB and NCB possess different and specific yeasts communities. Some yeasts species isolated from A. cerana show a low degree of similarity to their closest related species. Our study sheds light on the detection of several new taxa of yeasts associated with A. cerana.
Identification and Phylogenetic Analysis of Bacterial Isolates from Litopenaeus vannamei Shrimp Culture System and Gut Environment Based on 16SrRNA Gene Sequence Data RAHAYU, TUBAGUS HAERU; GANDJAR, INDRAWATI; RIANI, ETTY; DJUNAIDAH, IIN SITI; SJAMSURIDZAL, WELLYZAR
Microbiology Indonesia Vol 3, No 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

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Selected bacterial isolates from a Litopenaeus vannamei shrimp culture system and gut environment were assessed using 16S rRNA gene sequencing method to identify their identity and to construct their phylogenetic relationship. In a preliminary study, a total of 19 isolates were selected as probiotics. These isolates were prepared using freeze and heat-shock method to obtain the DNA template. PCR amplification of 16S ribosomal RNA gene of isolates was carried out using bacterial universal primers 9F and 1510R and was sequenced using an automated DNA sequencer. These gene sequences were compared with other gene sequences in the GenBank database (NCBI) using a BLAST search to find closely related sequences. Alignment of these sequences with sequences available from GenBank database was carried out to construct a phylogenetic tree for these bacteria. Most of the isolates obtained, i.e. 17 out of the 19 isolates, belonged to different species of Bacillus, sharing 95 to 99% 16S ribosomal RNA identity with the respective type-strain, whereas the remaining 2 isolates belonged to Micrococcus sp. and Micrococcus luteus with 97 to 99% 16S rRNA homology, consecutively.
Ecological and Taxonomical Perspective of Yeasts in Indonesia SJAMSURIDZAL, WELLYZAR; OETARI, ARIYANTI; KANTI, ATIT; SARASWATI, RASTI; NAKASHIMA, CHIHARU; WIDYASTUTI, YANTYATI; KATSUHIKO, ANDO
Microbiology Indonesia Vol 4, No 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

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In the course of ecological and taxonomical study of yeasts in Indonesia, a total of 2147 yeast isolates from 315 samples in the year 2003, 2005, 2006, and 2008 had been obtained from eight locations e.g. Liwa (Sumatera), Cibinong (Java), Cibodas (Java), Kutai (Kalimantan), Enrekang (Sulawesi), Pucak (Sulawesi), Gili and Kuta (Lombok), and Kupang (Timor).  Leaves, flowers, litters, soils, epiphytic soils, insects and insect´s nests were collected for yeasts isolation.  Our molecular identification based on D1/D2 region of nuclear large-subunit rDNA and the internal transcribed spacer (ITS) regions sequence data on 525 representative isolates revealed that 306 isolates belong to 48 described species (18 genera) and 209 strains belong to 19 undescribed species (19 genera), and 10 isolates were discarded because of contamination.  Based on their substrates, litter had the highest yeasts genera (19) followed by soils (18), flowers (10), leaves (6), epiphytic soils (4), and insects and insect´s nests (4).  Genera found on soils were also common on litters.  Yeasts genera found on flowers and epiphytic soils were common on leaves and litters.  The genera Aureobasidium, Cryptococcus, Pseudozyma, Rhodotorula and Sporidiobolus were found in all substrates.  Based on their locations, Kutai had the highest number of genera (15) followed by Cibodas (10), Cibinong (10), Enrekang (10), Kupang (10), Pucak (9), Liwa (7), and Lombok (7).  The genus Cryptococcus was found in all locations.  Our study shed a light to detection of many new taxa of yeasts, 41% of yeasts found in this study represented novel taxa.
Genetic Diversity of Osmophilic Yeasts Isolated from Indonesian Foods with High Concentration of Sugar RIDAWATI, .; JENIE, BETTY SRI LAKSMI; DJUWITA, ITA; SJAMSURIDZAL, WELLYZAR
Microbiology Indonesia Vol 4, No 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

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Isolation of osmophilic yeasts from a total of 70 samples consisting of jam, sweet condensed milk, honey, sweet soy sauce, and palm sugar was conducted. Sixty-eight osmophilic yeasts were isolated from strawberry jam, pineapple jam, and honey from South Sumatera. No yeast was obtained from condensed milk, honey from Sumbawa, sweet soy sauce, and palm sugar. Sequence analysis based on the ITS region showed that isolates were identified as five species belong to two genera, Candida and Sterigmatomyces. Those isolates were distributed in 5 species, C. metapsilosis, C. etchellsii, C. parapsilosis, C. orthopsilosis, and S. halophilus. C. etchellsii was the predominant species in South Sumatera honey, while C. parapsilosis group was predominant species in jams. Those species were reported as osmophilic yeasts. In both jams and honey we found C. parapsilosis and C. metapsilosis, whilst C. orthopsilosis was found only in pineapple jam. Phylogenetic analysis based on sequence of ITS region showed that most of the osmophilic yeasts (67 out of 68 isolates) were located in the phylum Ascomycota and only one isolate Sterigmatomyces halophilus NN38 from pineapple jam was located in the phylum Basidiomycota.
PENGUJIAN KEMAMPUAN ANTAGONISTIK KHAMIR Rhodotorula spp. ASAL KEBUN RAYA CIBODAS TERHADAP KAPANG DARI TANAMAN TOMAT TERINFEKSI DENGAN CO-CULTURE Hafsari, Anggita R; Oetari, Ariyanti; Salamah, Andi; Sjamsuridzal, Wellyzar
JURNAL ISTEK Vol 5, No 1-2 (2011): ISTEK
Publisher : JURNAL ISTEK

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Aspergilus ochraceu and A. terreus had been known as mould that causes significant loss to quality of tomatoes. One of potential methods to control the development of this mould by using the antagonistic activity of Rhodotorula sp. Six strains of Rhodotorula sp., UICC Y-318, UICC Y-325, UICC Y- 332, UICC Y-381, UICC Y-384, and UICC Y-386, which isolated from Cibodas Botanical Garden, West Java were observed for its antagonistic activity against mould A. ochraceu D1.2.2.2.SSM3 and A. terreus D2.2.MC. Co-culture method was used as 1.58 to 5.59 x 108 CFU/ml Rhodotorula sp. growth together with 7 x 107 CFU/ml A. ochraceus D1.2.2.2.SSM3 and 7.5 x 107 CFU/ml A. terreus D2.2.MC at PDB. Result showed that the highest percentage reduction of conidial heads and hyphal width was show by Rhodotorula sp. UICC Y-318 against A.ochraceus (9.45% and 12.43%; 7.10% and 7.51%, 2 and 3 day after incubation, respectively).
Insecticidal Activities of Ethyl Acetate Extract of Indonesian Mangrove Fungus Emericella nidulans BPPTCC 6038 on Spodoptera litura ABRAHAM, SILVA; BASUKRIADI, ADI; PAWIROHARSONO, SUYANTO; SJAMSURIDZAL, WELLYZAR
Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.391 KB) | DOI: 10.5454/mi.9.3.1

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Mangrove fungi are known as sources of biological active compounds. The study and the report of secondary metabolites of mangrove fungi as insecticides is very limited in Indonesia. This study assess the insecticidal activities of ethyl acetate extract of Indonesian mangrove fungus Emericella nidulans BPPTCC 6038 against Spodoptera litura (Lepidoptera, Noctuidae) neonate larvae and pupae. The fungus E. nidulans BPPTCC 6038 was isolated from leaves of mangrove Rhizophora mucronata and identified based on ITS rDNA sequence data, with the GenBank accession number KP165435, and confirmed with morphological observation. This fungus strain was grown on malt extract broth for 14 days on rotary shaker at 65 rpm, and incubated at room temperature. Mortalities of S. litura were observed on larvae fed on artificial diet containing ethyl acetate extract of E. nidulans at concentrations of 625 – 5000 ppm. The lethal concentration of the extract which causes 50% mortality of larvae (LC50 value) was 1102.27 ppm. The other effects of fungus extract on S. litura were decrease in growth rate, longer larval period, inhibition on pupal development and absence in adult emergence. The HPLC analysis of extract showed that the crude extract contained three major compounds. This study provides evidence that the extract of E. nidulans possesses insecticidal activities against S. litura.