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Seleksi Genotipe Padi Mutan Insersi Toleran Cekaman Salinitas Berdasarkan Karakter Pertumbuhan dan Biokimia Situmorang, Apriadi; Zannati, Anky; Widyajayantie, Dwi; Nugroho, Satya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Eleven genotypes of insertional mutant Nipponbare rice carrying randomly inserted activation tag were tested to investigate the growth and biochemical responses to salt stress. Pokkali, IR 29, and non-transgenic Nipponbare were used as tolerant, susceptible, and isogenic control, respectively. Plants were grown in plastic trays filled with 0  g L-1 NaCl  Yoshida nutrient solution as control and grown in 6 g L-1 NaCl as treatment. Salt injury was scored on 10, 16, and 21 days after salinization. Observation at 21 days after salinization showed that there was significant  difference  among genotypes in growth and biochemical characters. Classification  using hierarchical cluster analysis based on growth and biochemical responses showed that there were three clusters. Cluster I consisted of wild-type Nipponbare, T2.pMO.V.3.13.c.22, T2.pMO. V.1.7.a.15, T2.pMO.III.98.b.7, T2.pMO.II.231.c15, T2.pMO.III.98.b.8, T2.pMO.III.98.b.17, T2.pMO.III.98.b.3, and T2.pMO.VI.81.3.a.4. Cluster II consisted of IR 29, T2.pMO.V.3.13.c.14, T2.pMO.VI.30.1a.125, and T2.pMO.VI.30.1.a.65. However no mutant genotype was clustered with Pokkali in cluster III, which may indicate that there was no salt tolerant mutant genotype found in this research.   Keywords: salt tolerance mutant rice, growth characters, biochemical characters
PENGGUNAAN BERBAGAI JENIS BAHAN PEMANIS BUATAN PADA PEMBUATAN JAM PEPINO Pandiangan, Maruba; Situmorang, Apriadi
MEDIA UNIKA - Majalah Ilmiah Vol 1, No 2 (2013)
Publisher : Universitas Katolik Santo Thomas Sumatera Utara

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh jenis dan konsentrasi bahan pemanis buatan terhadap mutu jam pepino. Bahan yang digunakan dalam penelitian ini adalah buah pepino yang diperoleh dari supermarket Berastagi, pemanis buatan aspartam, asesulfam-K, sukralosa, sakarin, tepung maizena, gum arab, garam, jeruk nipis, vanila dan gula. Penelitian ini dilaksanakan di Laboratorium Pengolahan Hasil Pertanian Universitas Katolik Santo Thomas Sumatera Utara Medan. Penelitian ini dilakukan dengan rancangan acak lengkap (RAL) faktorial, yang terdiri dari dua faktor perlakuan. Perlakuan pertama yaitu jenis pemanis yang terdiri dari P1 = aspartam, P2 = asesulfam-K, P3 = sukralosa dan P4 = sakarin. Faktor kedua yaitu konsentrasi pemanis yang terdiri dari K1 = 0,02 %, K2 = 0,04 %, K3 = 0,06 % dan K4 = 0,08 %. Hasil penelitian menunjukkan bahwa kadar vitamin C, total asam, total padatan terlarut dan nilai organoleptik tertinggi terdapat pada jenis pemanis sukralosa, sedangkan kadar air tertinggi terdapat pada jenis pemanis Sakarin. Semakin tinggi konsentrasi zat pemanis kadar vitamin C, total padatan terlarut dan nilai organoleptik semakin meningkat, sedangkan total asam dan kadar air semakin menurun. Untuk memperoleh mutu jam pepino yang baik, dapat dilakukan dengan menggunakan pemanis sukralosa atau sakarin dengan konsentrasi 0,08 %.
IDENTIFIKASI GALUR-GALUR PADI MUTANINSERSI TOLERAN DAN RENTAN CEKAMAN SALINITAS BERDASARKAN KARAKTER MULTIVARIAT PERTUMBUHAN DAN BIOKIMIA PADA FASE VEGETATIF Situmorang, Apriadi; Zannati, Anky; Widyajayantie, Dwi; Nugroho, Satya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

Growth and biochemical characters of thirty-three Nipponbare-based rice mutant lines independently harboring activation-tag in salt stress condition were investigated. Rice cv Nipponbare wild type, IR29 and Pokkali were used as isogenic, susceptible, and tolerant cultivars, respectively. Plants were grown in Yoshida nutrient solution containing NaCI (6 g/1) as the stress treatment and in Yoshida nutrient solution without NaCI as the control. Evaluation of salt tolerance was conducted at 21 days after salinization.The results showed that there was a significant difference in growth characters among genotypes in reduction of the plant height,root length, shoot fresh weight, root fresh weight, shoot dry weight, as well as in the root dry weight. Biochemical characters of the genotypes also showed significant difference in their reduction of chlorophyll-a and chlorophyll-b concentrations,total carotenoids and proline accumulation. Based on multivariate growth and biochemical characters,T3.PMO.VI.63.5a.30.9,T3.PMO.VI.30.1a.21.1, T3.PMO.V1.63.5a.33.7F3.PUR.IX.49.1d.l.6.5,T3.PMO.VI.81.3a.4.4, F3.PUR.IX.49.1d.l.6.5,and F3.PUR.VIII.5.1f.l.4.8 were assumed as potential salt-tolerant lines, respectively, while mutant lines T3.PMO.VI.30.1a.l07.7,T3.PMO.VI.63.5a.20.6,T3.PMO.III.4.4c.7.2,andT3.PMO.VI.30.1a.51.1 were assumed as salt-susceptible lines respectively.
Phenotypic Screening of Ds transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayantie, Dwi; Pantouw, Carla F.; Astuti, Dwi; Indrayani, Sri; Rahmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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Abstract

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice,  combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller.   Keywords: rice, insertion mutant, gene discovery, drought, salinity