Usman Siswanto
Fakultas Pertanian Universitas Bengkulu

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Biomass and Artemisinin Production of Artemisia annua L. on Several Altitudes Nusantara, Abimanyu Dipo; Bertham, Yudhy Harini; Siswanto, Usman; Andani, Apri
International Journal on Advanced Science, Engineering and Information Technology Vol 9, No 2 (2019)
Publisher : International Journal on Advanced Science, Engineering and Information Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18517/ijaseit.9.2.2023

Abstract

Increasing supply of artemisinin as a multipurpose medicinal compound, especially to cope with malaria, is a global problem that should be solved immediately. The aim of this research was to obtain high artemisinin production from Artemisia annua L. cultivated at the suitable altitude at Bengkulu Province of Indonesia. Experimental design used was a Split Plot in Randomized Complete Block Design with three replications, altitude (1000, 1100 and 1200 m above sea level altitudes each located at Sukasari, Bandung Baru, and Tangsi Duren, Kepahyang District Province Bengkulu of Indonesia) as main plot and bio-fertilizer application (control, arbuscular mycorrhizal fungi, and phosphate solubilizer fungi) as subplot. Each experimental unit received 10 ton ha-1 of cow-dung. Research result shows that altitude significantly affects artemisinin, essential oil, mycorrhizal and phosphate solubilizer fungus population, but not affect biomass production. Overall, Artemisia annua L. was successfully planted at the 1000 – 1200 m above sea level. Biomass and artemisinin production was higher compared to other experiment, i.e 350 g per plant with 0.21 – 0.43% of artemisinin content.
The Study of Artemisinin Content in Callus Artemisia annua L. Cultures Elicited with Endophytic Fungi Aspergillus sp. Yuliani, Farida; Dewi, Widyatmani Sih; Yunus, Ahmad; Siswanto, Usman
Molekul Vol 13, No 2 (2018)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.067 KB) | DOI: 10.20884/1.jm.2018.13.2.459

Abstract

Artemisinin, is a sesquiterpene lactone which is originally extracted from the medicinal plant Artemisia annua L. Artemisinin is a very effective antimalarial agent, however it is produced in very low amounts in plants. Chemical synthesis of artemisinin is complicated and not economically feasible. Researchers often use elicitation methods to increase artemisinin content in a laboratory scale with biotic or abiotic elicitor. This study examined the effect of elicitation using endophytic fungi Aspergillus sp on artemisinin content of callus A. annua cultures. Observations were made on its growth, texture and color, chlorophyll content and dry weight. Fungi were isolated from the stem tissue of A. annua and callus were grown on Murashige and Skoog medium. After 3 weeks of incubation, callus treated with four different elicitors concentration (0, 2, 4, 6 mg/mL). There were significant alterations on callus culture in terms of growth, texture, color and chlorophyll content after elicitation. The results clearly demonstrated that the endophytic fungal elicitor Aspergillus sp could increase artemisinin content but did not affect the callus dry weight of A. annua. The treatment of 4 mg/mL  elicitor produced the highest artemisinin content, that increased 7.6 times compared to the control, from 0.0036% to 0.0275%.