Yulia Sistina
Jurusan Perikanan Fakultas Biologi Universitas Soedirman

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Deteksi Triploid Ikan Nilem (Osteochilus hasselti Valencienes 1842) Hasil Kejut Dingin 4oC Susanti, Desi; Yuwono, Edy; Sistina, Yulia
Biota BIOTA Volume 17 Nomor 3 Tahun 2012
Publisher : PBI Yogyakarta

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Abstract

Protokol triploidisasi cyprinidae nilem dengan kejut dingin 4oC pada 1, 3, atau 5 menit dari waktu fertilisasi dengan lama kejut 15, 20, atau 25 menit terbukti efektif dari parameyter fertilitas, daya tetas, abnormalitas larva, sintasan benih, panjang benih, dan khususnya data dimensi eritrosit benih hasil perlakuan. Dimensi eritrosit berupa ukuran panjang (major axes), lebar (minor axes), luas, dan volume eritrosit. Benih hasil perlakuan kejut dingin terbukti triploid dengan dimensi eritrositnya secara sangat nyata (P<0,01) jauh lebih besar dibanding diploid normal kontrolnya. Perlakuan kejut dingin secara sangat nyata (P<0,01) mempengaruhi fertilitas telur dan panjang tubuh benih, namun tidak nyata (P>0,05) mempengaruhi persentase penetasan, persentase abnormalitas larva, dan sintasan benih. Penerapan protokol triploid kejut dingin nilem yang terbukit efektif untuk pada bidang akuakultur siap dijalankan.Kata kunci: Deteksi triploid, kejut dingin, lama kejut, nilem, dimensi eritrosit
POLIPLOIDISASI IKAN NILEM (Osteochilus hasselti Valenciennes, 1842) DENGAN KEJUT DINGIN 40 CPOLYPLOIDIZATION ON SHARK MINNOW (Osteochilus hasselti Valenciennes, 1842) BY COLD SHOCK 40 C Fitria, Syawalina; Sistina, Yulia; Sulistyo, Isdy
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Penelitian ini bertujuan untuk mengetahui tingkat keberhasilan poliploidisasi, yakni triploidisasi dan tetraploidisasi  ikan nilem (Osteochilus hasselti Valenciennes, 1842) menggunakan kejut dingin 40C. Variabel utama adalah persentase penetasan telur, kelangsungan hidup, abnormalitas dan dimensi sel darah merah  benih ikan nilem serta kualitas air sebagai variabel pendukungnya. Penelitian dilakukan dengan tujuh perlakuan dan empat ulangan. Telur dan milt encer segar difertilisasi, pada 5, 20 atau 25 menit setelah fertilisasi dikejut temperatur dingin 40C selama 20 atau 30 menit. Persentase penetasan dihitung 24 jam setelah fertilisasi. Abnormalitas, kelangsungan hidup dan penentuan tingkat ploidi dengan pengukuran dimensi sel darah merah dihitung pada benih ikan nilem umur 60 hari. Hasil penelitian menunjukkan bahwa abnormalitas, kelangsungan hidup dan dimensi sel darah merah benih ikan nilem berbeda sangat nyata (P<0,01), sedangkan penetasan telur dan laju pertumbuhan spesifiknya tidak berbeda nyata (P>0,05). Hasil uji lanjut membuktikan bahwa perlakuan 5 atau 20 menit pasca fertilisasi  dan durasi kejut 30 menit memberikan hasil yang secara nyata paling baik dibandingkan perlakuan lain. Lama kejut nampak efektif dari penelitian ini. Dengan demikian, kejut temperatur dingin 40 C pada poliploidisasi ikan nilem ini siap diterapkan pada bidang akuakultur.  Kata Kunci : Triploidisasi, Tetraploidisasi, Ikan Nilem, Kejut Dingin
Viability of Nilem’s Eggs Delayed in Oviposition after The Sign of Spawning Soeminto, Soeminto; Susatyo, Priyo; Sistina, Yulia
SAINTEK PERIKANAN Vol 2, No 1 (2006): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

This study reports eggs viability from nilem (Osthehilus hasselti CV) parental which their oviposition has been delayed up to 3 – 24 hours from first actual oviposition time. By 3 hours interval, the experimental has been set as completely randomized design with 9 treatments as delay time, i.e. 0, 3, 6, 9, 12, 15, 18, 21 or 24 hours delayed oviposition time, each from three different fish as eggs sources. Results showed that eggs viability were observed  from eggs up to 6 hours delayed oviposition  treatments from data of fertilization rate of the egg and their larvaes up to 10 days old post hatching. Results from treatment more than 6 hours delayed oviposition showed eggs cleavage, however they never developed further to larvae nor hatching. The 18 hours delayed  oviposition  eggs groups developed  up to first cleavage. Treatment more than 21hours delayed did not showed any cleavage, but cytoplasmic changes were detected. It can be concluded that delayed ovipositions of nilem eggs up to 6 hours delayed did not affected their viability, they  developed  and hatched as controls one as well as the larvae survive up to 10 days  examination. Delayed up to 18 hours incubation although showed some activity, but they  were  all never developed further than cleavage stage.   Key words: Osteochilus hasselti CV, oviposition , eggs and spermatozoon viability
Fekunditas Udang Galah (Macrobrachium rosenbergii ) Betina Pasca Ablasi Unilateral dan Suplementasi Vitamin E pada Pakan Nasution, Rachmawati; Sastranegara, Moh. Husein; Sistina, Yulia
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.135

Abstract

This study reports on giant freshwater prawn fecundity with or without unilateral ablation treatment combined with alpha tocopherol (vit E) during 3 month culture. This exsperimental study applied 8 combination treatments of 4 different vit E dosages, i.e. 0, 200, 400, and 600 IU per kg feed with or without unilateral ablation. The results showed that treatments very highly significantly different (p<0,01) in fecundity with the 200 IU vit E dose combined with unilateral ablation was the best (45.228 ± 3.867 egg) optimum resulted in fecundity of the treated females. The overall fecundities averages were ablation group with 0 IU = 41.835 ± 837 eggs; 200 IU = 45.228 ± 3.867 eggs; 400 IU = 43394 ± 1523 eggs ; and the 600 IU = 43.718 ± 2.255 eggs, and for the non ablation with vit E group were 0 IU = 45.293 ± 2.899 eggs, 200 IU = 44.106 ± 1.759 eggs, 400 IU = 46.623 ± 2.556 eggs, and 600 IU=51.824 ± 1.132 eggs. The analysis results also proven that ablation factor gave very highly significantly (p<0,01) and vit E factor separately and in combination with ablation significantly (p<0,05) determined the females fecundity. In conclution, combination of unilateral ablation and tocopherol effective improved fecundity and the combination of unilateral ablation and vit E 200 IU the optimum resulted in giant freshwater prawn females fecundity.
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhyncos) Setelah Penyimpanan dalam Medium Berbeda Dikombinasi Krioprotektan Kuning Telur Berbagai Konsentrasi Atifah, Yusni; Saleh, Dadang Mulyadi; Pramono, Hendro; Sistina, Yulia
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.118

Abstract

The purpose of this study is to asses motility and viability of domestic duck (Anas platyrhyncos) spermatozoa after 6 day storing in preservation medium of different egg yolk cryoprotectant concentration in three different medium Tris, PBS or Ringer lactate. In this experiment, we applied 3 combination of factors as treatments : extender (Tris, PBS or Ringer lactate), egg yolk (EY) concentration (0, 5, 10, 15 or 25%) and storage time (0,1,2,3,4,5,6 days). Spermatozoa was obtained by massage technique, ejaculate then were swup up in the extender, the swup-up sperm then were incubated in preservation medium as each treatments subjected to motility and viability assesment before and after storage at refrigerator temperature (50C). The results showed that sperm motility and viability were highly significantly different (P<0.01) among 107 treatments. Extender factor as well as storage duration time were highly significantly different (P<0.01) affected the motility and viability of the treated sperm. EY concentration was significantly (P<0.05) affected viability, however there were no factor (P>0.05) affected motility of the sperm. The best treatment resulted in promising quality having the highest motility of 46,67 ± 32,15 5 motile was from the treatment of 10% EY in PBS stored for 4 days. The best viability result was from the tretament of 25% EY in PBS after 4 days storage resulted in 50,00 ± 36,05 % of viabie sperm. In conclusion this protocol could be applied for artificial insemination trial.
Keberhasilan Ginogenesis Ikan Tawes (Barbonymus gonionotus Blkr.) pada Dua Dosis Iradiasi UV (λ 254 nm) dengan Kejut Panas 40 C Apriyani, Reni; Suryaningsih, Suhestri; Sistina, Yulia
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.346

Abstract

Laporan ini memverifikasi keberhasilan protokol tiga tahap ginogenesis tawes melalui inaktivasi genom jantan dengan iradiasi ultra violet (UV), fertilisasinya, dilanjutkan diploidisasi zigot dengan kejut panas (400 C) selama 60 detik. Eksperimen Rancangan Acak Lengkap, tujuh perlakuan yaitu, kontrol positif (fertilisasi normal); kontrol negatif1 telur difertilisasi dengan milt encer yang di UV 1983,348 J/m2; kontrol negatif2 dosis iradiasinya 3966,96 J/m2; ginogenesis1 kontrol negatif1 lalu dikejut panas pada 10 menit pasca fertilisasi; ginogenesis2 ginogenesis1 beda waktu kejut pada 15 menit pasca fertilisasi; ginogenesis3 kontrol negatif2 lalu dikejut pada 10 menit pasca fertilisasi; dan ginogenesis4 ginogenesis3 beda waktu kejut pada 15 menit pasca fertilisasi. Materi gamet segar diperoleh dari induk yang diinduksi GnRH analog dan domperindon dosis 1,5 ml/kg bobot induk intramuskuler, 6-10 jam sebelumnya. Milt segar diencerkan 100 x dalam larutan Ringer. Data dianalisis dengan uji Anova, menggunakan program SPSS. Hasil penelitian menunjukkan bahwa data fertilitas, data penetasan dan data kelangsungan hidup yang terbukti homogen (p>0,05), menunjukkan bahwa ketujuh perlakuan secara statistik fertilitas, penetasan, dan kelangsungan hidupnya tidak nyata (p>0,05) antar pelakuan. Data persentase morfologi larva abnormal tawes menunjukkan bahwa perlakuan yang dicobakan memberikan pengaruh yang sangat nyata (P<0,01). Secara keseluruhan dapat disimpulan bahwa, keempat perlakuan mitoginogenesis yang diujikan efektif menghasilkan larva mitoginogenesis ikan tawes walaupun efektifitasnya masih tergolong rendah. Efektivitas perlakuan dibuktikan dari signifikansi data morfologi larva abnormal dariklompok kontrol negatif membuktikan efektivitas dosis inaktivasi genetis jantan dan morfologi larva normal hasil perlakuan ginogenesis membuktikan efektivitas diploidisasi kejut panas tidak berbeda dari morfologi kontrol positif.
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhynchos) Setelah Penyimpanan Refrigerator dalam Ekstender Dikombinasi Berbagai Konsentrasi Krioprotektan Gliserol Amelia, Arie; Saleh, Dadang Mulyadi; Pramono, Hendro; Sistina, Yulia
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.116

Abstract

The economic value of ducks has increased one Day Old Duck in large quantities and short time, effects on artificial insemination technologies improvement. Spermatozoa used for artificial insemination, could be derived from sperm preservation biotechnology, by means to maintain or extend their viability and motility of those spermatozoa. Extenders and cryoprotectant are needed for maintaining sperm motility and viability during storage or preservation. This study aims to assess motility and viability of local ducks spermatozoa after six days srotrage in 3 different extenders (Ringers lactate, Tris or PBS) in combination with 5 different concentrations (0, 2, 4, 6, and 8%) of cryoprotectant glycerol. Results showed that sperm motility and viability was very highly significant (P<0.01) different among treatments, extender factor as well as duration storage time also very highly significantly (P<0.01) affected motility and viability of treated sperm. However, glycerol concentration factor was not significant (P>0.05) affected motility and viability of the sperm. The best treatment, resulted in the highest percentage motility and viability of sperm with progressivity score >1, was the 8% glycerol in PBS storage for up to 2 days (75.00 ± 21.79% and 71.67±14.43%). This protocol could be applied in artificial insemination trial.
Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture Rachmawati, Farida Nur; Affandi, Ridwan; Sistina, Yulia
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.843 KB) | DOI: 10.20884/1.oa.2018.14.2.546

Abstract

Eel population in nature reaches critical number, so that culture strategy is urgently needed to fulfil the high demand of this fish. A shortcut to get functional male, which proven difficult to be founded from natural catching, is masculinization. This research aimed to induce masculinization of tropical eel, Anguilla bicolor McClelland supplemented with various doses of 17α-methyltestosterone during a month culture in freshwater (0 ppt) or brackish water (10 ppt).  The eel was grouped and fed with supplementation of 17α-methyltestosterone at various doses, depending upon treatments, namely 0 mg Kg-1 diet (control), 40, 80 or 120 mg Kg-1 diet. Eels size were similar, at approximately 16,78 g ± 0,62 in weight and 25,38 cm ± 0,15 in length were either culture in freshwater or brackish during the experiment for eight weeks. Sex gonad, based on anatomical histological structures, Eye Index and Fin Index were measured after time culture treatment achieved, as well as body length, weight, eye diameter and the length of the pectoral fin were measured. Results showed that supplementation 17α-methyltestosterone 80 mg/Kg diet culture in brackish water has the highest number of male (90%).  This study proven that, the hormone was effective for masculinization in eels, It useful for masculinization in eels. Results proved that the 17α-methyltestosterone highly significant (P<0.01) effect on the Eye index (3.63 – 5.14) and Fin Index (3.03 – 4.08) of eels. This study concluded, that 17α-methyltestosterone more effective in improving the number of males in brackish water than in freshwater culture.
UKURAN ORGAN SISTEM REPRODUKSI ITIK JANTAN YANG DISUPLEMENTASI PROBIOTIK MEP+ BERBAGAI DOSIS SELAMA 30 HARI Rirgiyensi, Chelsy; Sistina, Yulia; Rachmawati, Farida Nur
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (769.21 KB) | DOI: 10.20884/1.sb.2014.1.3.39

Abstract

Probiotics MEP+ can increase fowl weight and weft efficiency, therefore it is important to know probiotics MEP+ effect at different dosage toward reproduction aspect. This research aimed to examine duck reproduction organ size suplemented with probiotics MEP+ with different dosage within 30 days. This research used Completely Randomized Design (CRD) with 4 treatments with different dosages within 30 days which was without probiotic’s application or control (K), 0,75 ml/kg wefts (P1) dose, 1,5 ml/kg wefts (P2) dose, a n d 3 ml/kg wefts (P3) dose. Each treatment repeated 8 times. Total 40 ducks raised in floor dry cage system. At 31st day of treatment duck reproduction system organ was measured. Whole results show increase average data (±SD) for weight of both right and left testis, and liver weight with highly probiotics dosage it, however the analysis result statistic not significant (P>0,05) except weight of right left testis with duck weight or gonadosomatic indeks (GSI) were very significant (P<0,01) among all treatment at different dosages was compared control. The results is confirmed that probiotic's MEP+ treatment with different dosages within 30 days gave no effect towards duck reproduction system organ size except to gonadosomatic indeks (GSI) male duck.
Deteksi Triploid Ikan Nilem (Osteochilus hasselti Valencienes 1842) Hasil Kejut Dingin 4oC Susanti, Desi; Yuwono, Edy; Sistina, Yulia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 3 (2012): October 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i3.149

Abstract

Protokol triploidisasi cyprinidae nilem dengan kejut dingin 4oC pada 1, 3, atau 5 menit dari waktu fertilisasi dengan lama kejut 15, 20, atau 25 menit terbukti efektif dari parameyter fertilitas, daya tetas, abnormalitas larva, sintasan benih, panjang benih, dan khususnya data dimensi eritrosit benih hasil perlakuan. Dimensi eritrosit berupa ukuran panjang (major axes), lebar (minor axes), luas, dan volume eritrosit. Benih hasil perlakuan kejut dingin terbukti triploid dengan dimensi eritrositnya secara sangat nyata (P<0,01) jauh lebih besar dibanding diploid normal kontrolnya. Perlakuan kejut dingin secara sangat nyata (P<0,01) mempengaruhi fertilitas telur dan panjang tubuh benih, namun tidak nyata (P>0,05) mempengaruhi persentase penetasan, persentase abnormalitas larva, dan sintasan benih. Penerapan protokol triploid kejut dingin nilem yang terbukit efektif untuk pada bidang akuakultur siap dijalankan.Kata kunci: Deteksi triploid, kejut dingin, lama kejut, nilem, dimensi eritrosit