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P16INK4A Expression In Cervical Carcinoma Young Age Sinuhaji, Immanuel; Siregar, Budiningsih; Lisnawati, Lisnawati
Journal of the Indonesian Medical Association Vol. 63 No. 1 January 2013
Publisher : Journal of the Indonesian Medical Association

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Abstract

Introduction. Cervical carcinoma is still the highest prevalent malignancy in Indonesia. Therefore, early identification of the high risk lesion is needed. Antibody p16INK4Ais frequently found in high grade lesion and invasive carcinoma. The aims of study was to compare p16INK4A expression in young and old age, due to increased efficacy of prevention and early identification of invasive lesion.Methods. This cross-sectional study was performed on 30 formalin-fixed parafin embeded specimen of squamous cell carcinoma orginated from patient with young age (<30 years) and old age (45-55 years) respectively. The immunohistochemical staining was conducted againts p16INK4A protein to compare both of group.Results: P16INK4A expression in both group showed a strong result, both in nucleus or cytoplasm. However, there was no statistical difference between the expression of p16INK4A in young age groups and old age (p=0,951).Conclusion: There is no association between p16INK4A expression in young and old age. J Indon Med Assoc. 2013;63:4-8 .Keywords: cervical carcinoma, human papilloma virus, p16INK4A.
KORELASI ANTARA MIB-1, AgNOR DAN APOPTOSIS CASPASE-3 DENGAN RESPONS KEMORADIOTERAPI PADA KANKER SERVIK Kurnia, Iin; Siregar, Budiningsih; Soetopo, Setiawan; Ramli, Irwan; Kurjana, Tjahya; ., Andriono; Tobing, Maringan DL; Suryawathi, Bethy; Tetriana, Devita
Jurnal Sains dan Teknologi Nuklir Indonesia Vol 14, No 1 (2013): Februari 2013
Publisher : BATAN

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Abstract

Salah satu cara pengobatankanker servik adalah dengan kemoradioterapi melalui pemberian radioterapi dan kemoterapisecara bersamaan pada kanker servik stadium lanjut lokal. Respons kemoradioterapidipengaruhi oleh faktor biologis yaitu kinetika sel yang terdiri dari proliferasi dan kematian sel.Pada penelitian ini dipelajari korelasi antara biomarker proliferasi sel kanker, yaitu AgNOR, MIB-1, dan ekspresi apoptosis jalur caspase-3 dengan respons kemoradioterapi pada kanker servik.Dua puluh satu sediaan mikroskopik jaringan kanker servik yang diambil dari biopsi pasiensebelum menerima tindakan kemoradioterapi diberi pewarnaan AgNOR, sedangkan deteksiMIB-1 dan apoptosis caspase-3 dilakukan dengan teknik immunohistokimia. Setelah selesaimenerima kemoradioterapi dilakukan pengamatan respons klinik dengan cara pelvic control.Hasil penelitian menunjukkan bahwa sebelum kemoradioterapi tidak ditemukan korelasi nilaiAgNOR, MIB-1 dengan apoptosis (p>0,05). Proliferasi sel yang diamati dengan AgNOR danMIB-1 sebelum kemoradioterapi tidak menunjukkan adanya korelasi dengan respons jaringankanker setelah kemoradioterapi, namun ekspresi apoptosis menunjukkan korelasi positifdengan respons kemoradioterapi. Indeks apoptosis caspase-3 yang diperoleh dapat dijadikanbahan pertimbangan pada penjadwalan kemoradioterapi kanker servik.
Caspase-3 can not be Used to Predict the Response to Neoadjuvant Chemotherapy Regiment PVB in Cervical Cancer Stage IB-IIA Ambari, Ediwibowo; Winarto, Hariyono; Sutrisna, Bambang; Siregar, Budiningsih
Indonesian Journal of Obstetrics and Gynecology Vol. 1 No. 3 July 2013
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

Objectives: To determine the factors that may be used as the prognostic parameter for the therapeutic efficacy of neoadjuvant chemotherapy, which can be used to revising the management of early stage cervical cancer patients with large lesions. Methods: This was a retrospective cohort study. The study was conducted in the Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine, University of Indonesia. The subjects were 15 cervical cancer stage IB2 and IIA patients with lesions’ size of > 4 cm, who would be treated with neoadjuvant chemotherapy, consisted of cisplatin 50 mg/m2, vincristine 2 mg/m2 and bleomycin 15 mg regiment. The patients’ response would be evaluated after completing 3 series of chemotherapy. Data was retrieved from medical records and cervical biopsy paraffin blocks and examined histopathologically using IHC staining to see expression of caspase-3 with histoscore assessment score. Data was analyzed by univariate, bivariate analysis.Results: Response to PVB neoadjuvant chemotherapy was found in 5 out of 15 patients. None of the clinicopathology variables can be used to predict response to therapy. Expression of caspase-3 as a marker of apoptosis, can not predict the response of the therapy before administrating neoadjuvant chemotherapy either. There is a significant difference between the levels of caspase-3 in epidermoid carcinoma with adenocarcinoma, with p value of 0.02 (RR 6;95% CI 1.69-21.26).Conclusion: Clinicopathologic factors and the expression of caspase-3 before getting chemotherapy neoadjuvant can not predict the succeed of the therapy.Keywords: caspase -3, clinicopathologic, early-stage cervical cancer lession in large, neoadjuvant chemotherapy response to therapy
Clinical-pathologic factors, as predictor of lymph nodes metastasis in cervical cancer stage IB and IIA Aziz, M. F.; Andrijono, Andrijono; Nuranna, Laila; Purbadi, Sigit; Mangunkusumo, Rukmini T.; Siregar, Budiningsih; Cornain, Santoso; Saifuddin, Abdul B.; Tjarta, Achmad; Sutrisna, Bambang
Medical Journal of Indonesia Vol 13, No 2 (2004): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.052 KB) | DOI: 10.13181/mji.v13i2.574

Abstract

The aim of this study was to identify possible predictor factors of lymph node metastases in patients with cervical cancer stage IB and IIA. Study was conducted between May 1996 and December 2001. There were 183 patients of cervical cancer with FIGO Stage IB and IIA who were underwent radical hysterectomy and lymphadenectomy. From those 158 patients could be evaluated, consisting 43 patients with node metastases 115 patients without metastases. Research design was case control study. Case was patients with node metastases and control was those without node metastases. Multivariate analysis was made after bivariate analysis. On bivariate analysis age < 39 years, diameter of lesion > 4 cm, stage IIA > 4 cm, histopathology moderate and poor differentiation, blood and lymphatic vessel invasion were independent variables for node metastases with p value ≤ 0.05. However, on multivariate analysis younger age, parity ≥ 4, diameter of lesion, histopathology adenosquamous, and lymph vascular invasion (+) as independent factors for node metastases with p value ≤ 0.05. Conclusion: Younger age, parity ≥ 4, stage IIA > 4 cm, diameter of lesion, histopathology adenosquamous, and lymph vascular invasion (+) were risk factors for node metastases and can be used as predictors. (Med J Indones 2004; 13: 113-8)Keywords: cervical cancer, radical hysterectomy, node metastases, case control study, predictor
Cytokeratin 8,18 and 19 expressions, epithelial shedding, and stromal extravasation in endometrium of norethisterone enanthate (NET-EN) progestogen contraceptive users (A preliminary report) Wonodirekso, Sugito; Hadisaputra, Wachju; Affandi, Biran; Siregar, Budiningsih; Rogers, Peter A.W.
Medical Journal of Indonesia Vol 7, No 1 (1998): January-March
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

[no abstract available]
Alteration of Subcellular Beta Catenin Expression in Normal Mucosa Adenoma and Carcinoma in Relation to Colorectal Carcinogenesis Abineno, Pamela Damaledo; Handjari, Diah Rini; Siregar, Budiningsih
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 11, NUMBER 2, August 2010
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Adenomatous polyposis coli (APC) gene mutation was found in up to 80% of cases of sporadic colorectal cancers and adenomas. Loss of APC protein function has been known as one of the early process in colorectal carcinogenesis. This event leads to the accumulation of beta catenin in the cytoplasm and nucleus and subsequently activates target genes that regulate cell proliferation and apoptosis. The aim of this study was to investigate the alteration of subcellular beta catenin expression in the progression of colorectal cancer. Method: This cross-sectional study was conducted with 30 paraffin-embedded tissue sections each of normal colorectal mucosa, adenomas and carcinomas. Alteration of beta catenin expression in membranous, cytoplasmic, and nuclear compartments were evaluated by immunohistochemical staining. Results: Beta catenin immunoreactivity was detected in all cases, of which 87 (96.7%) cases showed membranous expression, 78 (86.7%) cases had cytoplasmic and 51 cases (56.7%) had nuclear expression. Such results were statistically significant (p < 0.000). All normal colorectal epithelium showed membranous beta catenin expression with 18 (60.0%) cases showed cytoplasmic and no nuclear beta catenin expression was found. Strong cytoplasmic expression was found in 17 (56.7%) adenomas and 25 (83.3%) carcinomas; while strong nuclear expression was found in 12 (40.0%) adenomas and 17 (56.7%) carcinomas. There was no statistical significant association between beta catenin expression in the membranous, cytoplasmic and nuclear compartment with the degree of dysplasia or differentiation of tumor (p > 0.05). Conclusion: Altered subcellular expression of beta catenin occurs as the oncogenic process develops from adenoma into carcinoma. Such finding reflects the importance of beta-catenin in colorectal carcinogenesis. Keywords: beta catenin, colorectal cancer, adenoma, colorectal cancer progression
Recurrence-Free Survival Stage IB1-HA2 Intermediate Risk Group (Based on Kartu Delgado) Cervical Carcinoma after Radical Surgery and Adjuvant Radiotherapy Friadi, Andi; Purbadi, Sigit; Siregar, Budiningsih; Sutrisna, Bambang
Indonesian Journal of Obstetrics and Gynecology Vol. 2. No. 3 July 2014
Publisher : Indonesian Journal of Obstetrics and Gynecology

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AbstractObjective: To evaluate the benefits of adjuvant radiotherapy (ART) based on Kartu Delgado (simple form of Gynecologic Oncology Group (GOG)scoring system) aimed at women with early stage cervical cancer after radical su rgery.Method: Fifty patients were enrolled for this study. Twenty one patients from 2011-2012 were given ART following surgery based on thei r Kartu Delgado score from as follows: score <120 were designated for observation; score >120 were given ART.Their score and recurrence were compared with 29 patients who were treated in 2009-2010 (based on single prognostic factor).Result: We observed eighteen recurrences for the duration th is study. Thirteen patients from the period of 2009-2010 and five patients from the period of 2011-2012. Most recurrences occurred in patients from 2009-2010 with score> 120 but were not designated ART. Two-years recurrence-free survival (RFS) for subjects with score <120 who were designated observation was 76.23% whil e for score>120 with ARTwas 64.29%. Conclusion: Adjuvant radiotherapy given based on Kartu Delgado reduced the number of recurrences in women with stage IB-IIA cervical cancer after treated by surgery.Keywords: cervical carcinoma, intermediate risk, prognostic factor, radical hysterectomy
Correlation Between Akt and p53 Protein Expression and Chemoradiotherapy Response in Cervical Cancer Patients KURNIA, IIN; SIREGAR, BUDININGSIH; SOETOPO, SETIAWAN; RAMLI, IRWAN; KURJANA, TJAHYA; ANDRIONO, .; TOBING, MARINGAN DIAPARI LUMBAN; SURYAWATHI, BETHY; KISNANTO, TEJA; TETRIANA, DEVITA
HAYATI Journal of Biosciences Vol 21, No 4 (2014): Desember 2014
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Akt is a protein that is associated with cell proliferation and is expressed at high levels in cancer cells. Some research indicates it may play a role in increasing the resistance of cancer cells to chemotherapy treatment. P53 is a tumor suppressor protein that influences the cell cycle and apoptosis. The purpose of this study was to examine the relationship between the expression of Akt and p53 in cancerous tissue before chemoradiation treatment, and the clinical response to treatment of cervical cancer patients. Twenty microscopic tissue samples were taken from cervical cancer biopsies obtained from patients before cancer treatment. The tissue samples were stained with p53 and Akt antibodies via immunohistochemistry technique, to measure expression of both proteins. After completion of chemoradiotherapy, patients’ clinical response to treatment was determined using the pelvic control method. Our results revealed no correlation between expression of Akt and p53 index (P = 0.74) as well as between p53 Index and chemoradiotherapy clinical response (P=0.29). There was significant correlation between expression of Akt and cervical cancer chemoradiotherapy response (P = 0.03). There was no correlation found between p53 index and chemoradiotherapy clinical response (P = 0.29). High expression of Akt may related with high cell proliferation and resistance to chemoradiotherapy.
Pendeteksian Ekspresi Biomarker ERK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi Kisnanto, Teja; Wardani, Rina Tri; Siregar, Budiningsih; Amir, Mellova; Soetopo, Setiawan; Ramli, Irwan; Kurjana, Tjahya; Andrijono, Andrijono; S Hernowo, Bethy; DL Tobing, Maringan; Tetriana, Devita
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 1 (2016): Juni 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positifCervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive.
Pendeteksian Ekspresi Biomarker MNK Secara Semi Kuantitatif dan Kuantitatif Pada Kanker Serviks Sebelum Respon Kemoradioterapi Kisnanto, Teja; Wardani, Rina Tri; Siregar, Budiningsih; Amir, Mellova; Soetopo, Setiawan; Ramli, Irwan; Kurjana, Tjahya; Andrijono, A; Hernowo, Bethy S; Tobing, Maringan DL; Tetriana, Devita
Jurnal Keselamatan Radiasi dan Lingkungan Vol 1, No 2 (2016): Desember 2016
Publisher : Pusat Teknologi Keselamatan dan Metrologi Radisasi - BATAN

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Abstract

Kanker servik merupakan penyakit kanker yang umum dijumpai pada wanita yang disebabkan oleh virus HPV (Human Papilova Virus). Tujuan penelitian ini adalah untuk mengetahui hubungan ekspresi protein MNK (Mitogen Activated Protein Kinase) pada penderita kanker serviks sebelum tindakan pengobatan terhadap respon kemoradioterapi. Sampel uji yang digunakan adalah sediaan mikroskopis hasil biopsi jaringan kanker dari penderita kanker serviks stadium lanjut (IIB-IIIB) sebanyak 20 sampel. Metode yang digunakan adalah metode imunohistokimia dengan menggunakan biomarker MNK pada biopsi jaringan kanker serviks. Ekspresi protein MNK yang positif ditandai dengan warna coklat tua yang terdapat pada inti sel. Respon kemoradioterapi diperoleh dari RSUPN Dr. Cipto Mangunkusumo Jakarta dan RS Hasan Sadikin Bandung. Hasil penelitian menunjukkan nilai IRS (Imuno Reaktif Score) protein MNK pada grup respon kemoradioterapi baik lebih tinggi dibandingkan grup respon kemoradioterapi buruk dan tidak ditemukan adanya hubungan IRS protein MNK dengan respon kemoradioterapi. Sedangkan hubungan ekspresi MNK terhadap respon kemoradioterapi menunjukkan adanya korelasi perbedaan grup respon kemoradioterapi antara ekspresi protein MNK negatif dan ekspresi protein MNK positif. Cervical cancer is a cancer that common in women caused by HPV (Human Papilova Virus). The purpose of this study is to determine the relationship MNK protein expression (Mitogen-Activated Protein Kinase) in patients with cervical cancer before chemoradiotherapy treatment. Sample used was the preparation of microscopic cancer tissue biopsies from patients with advanced cervical cancer (IIB-IIIB) is 20 samples. The method used is immunohistochemistry using MNK biomarkers in cervical cancer tissue biopsies. MNK positive protein expression marked with dark brown color that is contained in the cell nucleus. Chemoradiotherapy response obtained from RSUPN Dr. Cipto Mangunkusumo and Hasan Sadikin Hospital in Bandung. The results show the value of the IRS (Immuno Reactive Score) MNK protein in response to chemoradiotherapy group either higher than the response to chemoradiotherapy group was bad and did not find any relationship IRS MNK protein with chemoradiotherapy response. While the relationship MNK expression responses show a correlation chemoradiotherapy group differences in chemoradiotherapy response between MNK expression negative and MNK expression positive.