Meity Suradji Sinaga
Departemen Proteksi Tanaman, Institut Pertanian Bogor

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Potensi Cendawan Endofit sebagai Agens Pengendali Hayati Phytophthora palmivora (Butl.) Butl. Penyebab Busuk Buah Kakao Tondok, Efi Toding; Sinaga, Meity Suradji; Widodo, ,; Suhartono, Maggy Thenawidjaja
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were sprayed with each EF propagules. The inoculated pods were harvested two weeks later and subsequently inoculated with P. palmivora. Scoring of disease development was performed and quantified as area under disease progress curve (AUDPC). Latent period, infection rate and effectiveness were also recorded. In vitro growth inhibition of pathogen and induced plant defense mechanisms due to EF were also investigated. Xylariaceaeand Calocybe gambosatreatment generated the highest effectiveness control level, i.e. 38.8% and 33.8% respectively, followed by Resinicium friabileand Aschersoniatreatment, i.e. 17.4% dan 12.7% respectively.  Pestalotiopsisand Fusariumwere not effective to control BPD. There was a strong connection between disease severity of BPD with the latent period of pathogen. Growth inhibition of pathogen and induced resistance of plant were partially responsible for disease suppression by Xylariaceae, C. gambosa, R. friabileand Aschersonia. Keywords: Calocybe gambosa, induced resistance, peroxidases, salicylic acid, Xylariaceae
Repellent Plants and Seed Treatments for Organic Vegetable Soybean Production Aziz, Sandra Arifin; Pardiyanto, Agus Yudhi; Sinaga, Meity Suradji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 1 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The research was conducted to study the effect of repellent plants and seed treatments on growth and  production of organically grown vegetable soybean.  The experiment was carried out at Cikarawang Research Station, Bogor, from September 2005 to May 2006. The organic experiment was arranged in a split plot design using four species of companion plants as repellent plants, i.e. Tagetes  erecta, Cymbopogon nardus, Ocimum gratissimum,  Tephrosia  vogelii, and without repellent plants as the main plot, and seed treatments i.e. galangal oil, Pseudomonas  fl  uorescens, and without seed treatments as sub plot using 3 replications and conventional system (using pesticides) as control.  Plants grown under conventional system had a greater fresh pod weight (6.7 kg. 10 m-2) than those in organic system (4.80-5.79 kg. 10 m-2), a lower insect infestation (19.17, 22.92 and 32.50%) and disease prevalence (9.17, 11.42 and 14.42%), at 6, 7 and 8 Week After Planting (WAP) respectively, than the organic system.  In the organic experiment, the use of O. gratissimum as repellent plants resulted in a signifi  cantly lowest empty pod per plant (0.79 g). T. erecta and O. gratissimum without seed treatment, P. fluorescens without repellent plants, and T.  vogelii  with galangal oil seed treatment has the signifi  cant lowest insect infestation at 6 WAP of 20.67, 23.00, 26.67 and 27.33%, respectively.  An organic system using repellent plants had a significantly lower insect infestation at 8 WAP (35.67-40.33%, O. gratissimum being the lowest) than without repellent plants (50.56%). Seed treatments on organic system had the lower disease prevalence at 8 WAP (33.87% on P. fluorescens and 35.47% on galangal oil) than without seed treatments (37.73%). Number of root nodules (11.6-16.7 to 7.8) and root nodules dry weight (0.068-0.101 to 0.040 g) of the organic system were greater than the conventional system.  Soybean without repellent plants had a greater number of harvestable plants (137.3), but it was fewer than the conventional system (158.3).    Keywords: disease and pest control, seed treatments, organic vegetable soybean
Keefektifan Perlakuan Panas Kering dan Iradiasi UV-C untuk Mematikan Cendawan Model Microcyclus ulei Cristin, Aprida; Sinaga, Meity Suradji; Adnan, Abdul Muin
Jurnal Fitopatologi Indonesia Vol 9, No 2 (2013)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

This study was carried out to develop treatment methods and determine the effective dose of dry heat treatment and UV-C irradiation to kill the fungus models of Microcyclus ulei on soybean grains and soybean meals without damaging its nutritional content. The fungus models used in this study were Colletotrichum gloeosporioides, Botryodiplodia theobromae, Fusarium oxysporum f. sp. niveum, and Sclerotium rolfsii. The results showed that UV-C treatment up to 12 and 24 hours exposure time at15 and 30 cm from UV-C light was not effective to kill all fungus models in PDA. Dry heat treatment at 55 °C for 30 and 60 minutes was effective to kill cultures of C. gloeosporioides, B. theobromae, and S. rolfsii in PDA, except for F. oxysporum f. sp. niveum. Further experiments on soybean showed that dry heat treatment at 55 °C for 30 minutes was effective to kill conidia of C. gloeosporioides, B. theobromae, and F. oxysporum f. sp. niveum, without damaging its protein content. However, dry heat treatment at 60 °C for 60 minutes has been proved not effective to kill sclerotia of S. rolfsii. If M. ulei had the similar resistance as S. rolfsii, then it would need a higher temperature and time than used in this study to kill the fungusKey words: Botryodiplodia theobromae, Colletotrichum gloeosporioides, effective doses, Fusarium oxysporum f. sp. niveum, Sclerotium rolfsii, soybean meals
Trichoderma dan Gliocladium untuk Mengendalikan Penyakit Busuk Akar Fusarium pada Bibit Kelapa Sawit Juariyah, Siti; Tondok, Efi Toding; Sinaga, Meity Suradji
Jurnal Fitopatologi Indonesia Vol 14, No 6 (2018): IN PRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

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Abstract

Trichoderma and Gliocladium for Controling Fusarium Root Rot Disease of Oil Palm SeedlingsFusarium spp. have been reported as the causal agent of common spear rot and crown rot diseases on oil palm.? An effective strategy to control these diseases is not available yet. This research was aimed to find biocontrol agents for effective control of crown rot disease on oil palm seedlings caused by Fusarium spp. The experiment consisted of 3 parts i.e. pathogenicity test of 3 isolates of Fusarium, identification and in vitro test of biocontrol agents, and in planta test of biocontrol agents against Fusarium spp. In vitro test was done through dual culture test and test for volatile compound produced by the biocontrol agents. In planta test was conducted through inoculation of Fusarium spp. into oil palm seedlings growing on medium containing? selected biocontrol agents i.e. Trichoderma harzianum, T. virens, T. inhamatum, and Gliocladium fimbriatum. In vitro test showed that Gliocladium fimbriatum 1 and 2 were inhibited effectively the growth of Fusarium spp. on the dual culture test, whereas T. harzianum Gadingrejo 2 was inhibited effectively the growth of Fusarium spp. on volatile compound test. The application of biocontrol agents was effective to protect oil palm seedlings from Fusarium spp. infection.
Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers KHAERUNI, ANDI; SUWANTO, ANTONIUS; TJAHJONO, BUDI; SINAGA, MEITY SURADJI
HAYATI Journal of Biosciences Vol 14, No 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

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Abstract

A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields. Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer
Identifikasi Penyebab Penyakit Busuk Pangkal Batang pada Jeruk Retnosari, Eka; Henuk, Julinda Bendalina Dengga; Sinaga, Meity Suradji
Jurnal Fitopatologi Indonesia Vol 10, No 3 (2014)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Research was initiated to identify the causal agent of basal stem rot disease from 11 citrus growing areas in Indonesia. Identification of fungal pathogens was based on macroscopic and microscopic observation of morphological characters. Koch Postulate was conducted to confirm the causal agent of the disease. Two fungal pathogens, Botryodiplodia theobromae, and Phytophthora citrophthora were found associated with basal stem rot disease. B. theobromaewere isolated from all plant samples, whereas P. citrophthorawas only isolated on samples from Soe (East Nusa Tenggara). Characteristic symptoms of basal stem rot disease was developed on plants inoculated with B. theobromae and P. citrophthora; this confirmed that two fungal isolates was the causal agent of basal stem rot disease.
Identifikasi Molekuler Fitoplasma yang Berasosiasi dengan Tanaman Kaktus Hias Opuntia sp. Prasetya, Ariny; Mutaqin, Kikin Hamzah; Sinaga, Meity Suradji; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol 13, No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

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Abstract

Cactus species (Opuntia sp.) is a popular ornamental succulent plant. Some ornamental cactus species in Indonesia showing proliferation and green mosaic pattern symptoms have been reported to be associated with phytoplasma infection. However, further molecular identification for accurate classification of the causal phytoplasma has not been done.? This study aimed to diagnose phytoplasma associated with Opuntia sp. based on molecular methods involving PCR standard combined with nested-PCR, cloning and DNA sequencing. Standard PCR was carried out using P1/P7 primers followed by nested-PCR using R16F2n/R16R2 or fU5/rU3 primer pairs which amplify the 16S rRNA gene targets of 1.2 kb and 880 bp, respectively. Amplified fragment of nested-PCR using R16F2n/R16R2 primers was chosen to be cloned and sequenced for further identification and classification of phytoplasma.? BLASTn analysis showed that the phytoplasma from Opuntia sp. was closely related to 16SrII group. Phylogenetic analysis and in silico RFLP indicated that phytoplasma strain infecting Opuntia sp. was a member of subgroup 16SrII-C (cactus witches? broom phytoplasma). This is a newly report of cactus witches? broom phytoplasma on Opuntia sp. in Indonesia.? ?
Perlakuan Udara Panas untuk Pengendalian Perkecambahan Spora Tilletia indica pada Gandum Handayani, Nurul Dwi; Setyawan, Taufan Tanto; Salbiah, Salbiah; Wahyuno, Dono; Sinaga, Meity Suradji
Jurnal Fitopatologi Indonesia Vol 14, No 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

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Abstract

Karnal bunt of wheat (Triticum aestivum) caused by Tilletia indica is a major concern in international trade because it can degrade quality significantly, and it is a quarantine pest for Indonesia. Tilletia infected seeds can be the source of fungal inoculum of other areas. The current research was aimed to determine the effectiveness of temperature and exposure time to control Tilletia using air heat treatment without reducing the quality of grain. The experiments were conducted in vitro, initiated by seed testing, including morphology-based detection and identification of Tilletia followed by a viability test of the seed. Air heat treatment was performed by adjusting the oven temperature at 75, 80, 85 ?C and 28 ?C (control) for 4 and 6 hr exposure time, each with three replications. The results showed that air heat treatment of the wheat at 75 ?C for 4 hr was able to suppress teliospores germination of 56.7?61.7%, without denature protein content of the wheat.?
Antagonism Mechanism of Epiphytic Yeast against Anthracnose Pathogen (Colletotrichum acutatum) on Chilli Hartati, Sri; Wiyono, Suryo; Hidayat, Sri Hendrastuti; Sinaga, Meity Suradji
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019): In Press
Publisher : Universitas Gadjah Mada

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Abstract

Epiphytic yeasts have the potency as antagonistic agents against various pathogens of post-harvest products. Anthracnose is a major disease of chilli that causes high economic loss. This research was objected to study the antagonism mechanism of epiphytic yeast isolates that have the antagonistic potency against anthracnose pathogen on chilli (Colletotrichum acutatum). Twenty-two isolates of epiphytic yeasts, isolated from chilli leaves and fruit, were tested. The characterization of the antagonism was carried out by antibiosis, anti-fungus volatile production, and chitinolytic activity tests. The results showed that all tested isolates did not have antibiosis mechanism against C. acutatum. All isolates produced volatile compounds which inhibited the colony growth of C. acutatum. Four isolates showed high relative inhibition rate, i.e. isolates B32DEP (35.68%), B30DEP (37.52%), B23DEP (38.52%), and B29DEP (45.42%). Fourteen isolates showed chitinolytic activities. Three of them had high chitinolytic activities, i.e. B12DEP, B2DEP, and G237DEP.
Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were Tondok, Efi Toding; Sinaga, Meity Suradji; Widodo, ,; Suhartono, Maggy Thenawidjaja
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were sprayed with each EF propagules. The inoculated pods were harvested two weeks later and subsequently inoculated with P. palmivora. Scoring of disease development was performed and quantified as area under disease progress curve (AUDPC). Latent period, infection rate and effectiveness were also recorded. In vitro growth inhibition of pathogen and induced plant defense mechanisms due to EF were also investigated. Xylariaceaeand Calocybe gambosatreatment generated the highest effectiveness control level, i.e. 38.8% and 33.8% respectively, followed by Resinicium friabileand Aschersoniatreatment, i.e. 17.4% dan 12.7% respectively.? Pestalotiopsisand Fusariumwere not effective to control BPD. There was a strong connection between disease severity of BPD with the latent period of pathogen. Growth inhibition of pathogen and induced resistance of plant were partially responsible for disease suppression by Xylariaceae, C. gambosa, R. friabileand Aschersonia. Keywords: Calocybe gambosa, induced resistance, peroxidases, salicylic acid, Xylariaceae