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Journal : Forum Pasca Sarjana

Isolation and Identification of Serum Gamma Immunoglobulin (IgG) of Native and Imported Chickens By Ion Exchange Chromatography and Immunochernistry Methods Simorangkir, Murniaty; Girindra, Aisjah; Pasaribu, Fachriyan H.; Manalu, Wasmen
Forum Pasca Sarjana Vol 18, No 1 (1995): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

The study was designed to isolate and identify serum IgG of native and imported chickens, after being immunized with Newcastle Disease Vaccine. The isolation method used was the DEAE-Cellulose ion exchange chromatography using 0.01 M Tris-HCl buffer, at pH 8.0, with linear gradient NaCl from 0.01 M to 0.30 M after salting out with anhydrous Na2SO4. Identification of IgG characteristics carried out using the Ouchterlony double immunodiffusion, immuno-electrophoresis and SDS-PAGE 8% methods. Serum fractionation of native and imported chickens using the DEAE-Cellulose chromatography, after salting out with anhydrous Na2So4 of 18,14 and 14% resulted in four peaks of protein fractions.