Articles

Antioxidant Activity of Stem Bark Ethanolic Extracts of Calophyllum pulcherrimum, C. soulattri, and C. teysmannii Septiana, Eris; Simanjuntak, Partomuan
Buletin Penelitian Tanaman Rempah dan Obat Vol 29, No 2 (2018): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v29n2.2018.59-68

Abstract

The decreased air quality due to severe pollution causes the formation of free radicals in the body. Antioxidants counteract free radicals compounds and are beneficial to health. Calophyllum plants is one of the natural antioxidants source. The study was aimed to determine the best antioxidant activity of ethanolic extracts of stem bark both single and combinations of three species of Calophyllum. The study was conducted from September to December 2017 at the Natural Product Chemistry Laboratory, Research Center for Biotechnology LIPI, Cibinong, West Java. The experiment consisted of seven treatments, repeated three times.  The treatments consisted of ethanolic stem bark extracts of a) C. pulcherrimum b) C. soulattri (CS), c) C. teysmannii (CT), d) combination of C. pulcherrimum and C. soulattri (CP+CS), e) combination of C. pulcherrimum and C. teysmannii (CP+CT), f) combination of C. soulattri and C. teysmannii (CS+CT), and g) combination of C. pulcherrimum, C. soulattri and C. teysmannii (CP+CS+CT) with the ratio of mixed extract was 1 : 1 for treatments with two combinations and 1 : 1 : 1 for three combinations. The antioxidant activity was evaluated using DPPH free radical scavenging  methods.  The  total  phenolic   and   flavonoids   content   assays   were conducted base on the reaction of follin-ciocalteu and aluminum chloride reagents respectively.  The results showed that combination of Calophyllum stem bark ethanolic extracts revealed higher antioxidant activity, total phenol and flavonoid content compared to its sole extract. CP+CS+CT treatment has the lowest IC50 value at 3.12 mg.l-1 so that it has the potential as a source of natural antioxidants.
TOKSISITAS, AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK AIR KULIT KAYU MASSOI (CRYPTOCARPA MASSOY (LAURACEAE)) Lohita Sari, Bina; Rurianti, Wandesta; Simanjuntak, Partomuan
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 4, No 1 (2014): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.548 KB) | DOI: 10.33751/jf.v4i1.183

Abstract

Massoi (Cryptocarya massoy) merupakan tanaman yang digunakan masyarakat Papuasebagai obat tradisional. Kulit batang tanaman ini memperlihatkan beberapa aktivitasbiologis. Penelitian ini bertujuan untuk menentukan toksisitas, aktivitas antioksidan danantibakteri ekstrak air kulit batang Massoi (EAKM). Uji toksisitas menggunakan metodeBrine Shrimp Lethality Test (BSLT), aktivitas antioksidan diuji dengan metode PeredamanRadikal Bebas menggunakan DPPH (1,1-diphenyl-2-picrylhydrazyl), dan uji antibakterimenggunakan metode cakram difus terhadap Staphylococcus aureus dan Escherichia coli.Penapisan fitokimia menunjukkan adanya steroid, flavonoid, saponin, tannin, kumarin danminyak atsiri. Hasil LC50sebesar 493,00 g/mL menunjukkan bahwa EAKM adalah toksik.Nilai IC50sebesar 14,06 g/mL (vitamin C sebagai control positif 7,78 g/mL) menunjukkanpotensi EAKM sebagai antioksidan. Sementara EAKM tidak menunjukkan aktivitasantibakteri terhadap S. aureusdan E. coli. Kromatografi kolom menggunakan silika geldengan fasa gerak kloroform:metanol (1:1) dan kloroform:metanol:air (5:5:1) pada EAKMmenghasilkan empat fraksi. Semua fraksi diidentifikasi dengan KCKT menggunakan fasagerak metanol : air (1:1). Profil KCKT keempat fraksi menunjukkan profil kromatogram yanghampir sama, yaitu pada waktu menit ke 10,0.Kata kunci :Toksisitas, antioksidan, antibakteri, Cryptocarya massoy
THE POTENTIAL OF ENDOPHYTIC FUNGAL EXTRACT ISOLATED FROM CINNAMON (CINNAMOMUM BURMANNII) AS ANTIDIABETIC AND ANTIOXIDANT Septiana, Eris; Rachman, Fauzy; Hapsari, Yatri; Yadi, Yadi; Bustanussalam, Bustanussalam; Rahmawati, Siti Irma; izzati, Fauzia Nurul; Simanjuntak, Partomuan
Jurnal Kimia Sains dan Aplikasi Vol 22, No 6 (2019): Volume 22 Issue 6 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3558.055 KB) | DOI: 10.14710/jksa.22.6.275-282

Abstract

An increase in blood glucose levels in people with diabetes can cause an increase in free radicals, which can worsen the disease. Thus, drugs that have antidiabetic and antioxidant activities are needed. The cinnamon plant is high in antioxidants and has long been used as a source for a diabetes drug. The utilization of endophytic fungi isolated from cinnamon plants as antidiabetic and antioxidant has never been reported. This study aims to investigate the antidiabetic as well as antioxidant activity from the extract of endophytic fungi from the cinnamon plant. The antidiabetic activity was tested using the ?-glucosidase enzyme inhibition method, while antioxidant activity was tested using the DPPH free radical scavenging method. Total phenol content was measured based on the Follin-Ciocalteu reagent reaction. All endophytic fungal extracts from the cinnamon leaves, twigs, flowers, and fruit have antidiabetic and antioxidant activity as well as high total phenol content. The three parameters measured showed a positive correlation. Endophytic fungal extract of Cb.D6 isolate derived from the leaf had the highest antidiabetic and antioxidant activity among the other isolates amounting to 92.41% and 90.28%, respectively. In addition, the total phenol content of Cb.D6 isolates was also the highest with 357.83 mg equivalent to gallic acid/g extract. Therefore, the endophytic fungal extract of Cb.D6 isolate has the potential to be developed as a source of the antidiabetic and antioxidant ingredients.
PENGARUH KONDISI KULTUR YANG BERBEDA TERHADAP AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER KAPANG ENDOFIT ASAL AKAR KUNYIT Septiana, Eris; Simanjuntak, Partomuan
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.699 KB) | DOI: 10.22146/tradmedj.24308

Abstract

Antioksidan merupakan topik yang menarik karena kemampuannya dalam meredam radikal bebas dan mencegah kerusakan akibat oksidasi. Kapang endofit adalah salah satu sumber senyawa antioksidan di alam. Rendahnya hasil dalam memperoleh senyawa antioksidan dari kapang membuka peluang untuk mencari komposisi media dan kondisi pertumbuhan yang optimal. Penelitian ini bertujuan untuk mengetahui pengaruh kondisi media yang meliputi sumber karbon dan nitrogen serta pH awal fermentasi yang berbeda terhadap aktivitas antioksidan kapang endofit Bo.Ci.Cl.A3. Metode fermentasi yang digunakan ialah fermentasi goyang dengan kecepatan 120 rpm pada suhu ruang selama 14 hari. Sumber karbon yang digunakan ialah glukosa, sukrosa, dan pati. Sedangkan sumber nitrogen yang digunakan ialah NaNO3, NH4NO3, dan yeast extract. Kondisi pH awal yang digunakan ialah pH 5, 7, dan 9 dengan etil asetat sebagai bahan ekstraksi. Hasil yang didapatkan yaitu kapang endofit mampu menghasilkan senyawa metabolit sekunder antioksidan pada semua variasi media fermentasi yang digunakan. Sumber nitrogen yeast extract dapat meningkatkan aktivitas antioksidan kapang endofit Bo.Ci.Cl.A3, sedangkan sumber nitrogen, sumber karbon, dan pH awal yang berbeda dari media basal yang digunakan tidak memberikan peningkatan aktivitas antioksdian. Sehingga kesimpulannya ialah bahwa penggantian sumber nitrogen berupa yeast extract (3g/L) pada medium basal Czapek Dox’s Broth dapat meningkatkan kemampuan aktivitas antioksidan kapang endofit Bo.Ci.Cl.A3.
TUMBUHAN SEBAGAI SUMBER ZAT AKTIF ANTIMALARIA Simanjuntak, Partomuan
Buletin Penelitian Kesehatan Vol 23, No 2 Jun (1995)
Publisher : Buletin Penelitian Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | http://ejournal.litbang.depkes.go.id/index.php/BPK/article/view/186

Abstract

Malaria is a common disease caused by infection of Plasmodium spp. Based on retrievals in library reference it has been discovered that a number of chemical compounds, isolated from fruit-bearing plants, contain anti-protozoic active substances. These compounds are, among others, found in chemical structures of alkaloide, terpene, quinone, and phenolic. Research on traditional medicinal plants is required to investigate their potential for antimalarial drug. Study on the chemical structure of the bioactive compounds produced by plants is also important to obtain new drugs which may be more useful.
AKTIVITAS ANTIMIKROBA DAN ANTIOKSIDAN EKSTRAK BEBERAPA BAGIAN TANAMAN KUNYIT (Curcuma longa) Septiana, Eris; Simanjuntak, Partomuan
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 5, No 1 (2015): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.447 KB)

Abstract

Kunyit (Curcuma longa) merupakan tanaman obat tradisional yang biasa digunakan sebagai bumbu masakan dan sebagai bahan obat meliputi antimikroba, antioksidan,antitumor, dan anti inflamasi. Tujuan dari penelitian ini ialah untuk mengetahui aktivitas antimikroba dan antioksidan dari beberapa organ tanaman kunyit meliputi akar, rimpang, batang, dan daun. Semua bagian diekstraksi dengan etanol dan etil asetat. Seluruh ekstrak etanol dan etil asetat diuji aktivitas antimikrobanya menggunakan metode difusi cakram kertas terhadap Escherichia coli,Staphylococcus aureus, dan Candida albicans. Kloramfenikol dan nistatin masing-masing digunakan sebagai kontrol positif untuk uji antibakteri dan antijamur, sedangkan masing-masing pelarut untuk ekstraksi juga digunakan sebagai kontrol negatif. Aktivitas antioksidan dilakukan menggunakan metode 1,1-difenil-2pikril hidrazil (DPPH) dan asam askorbat digunakan sebagai standar. Hasil aktivitas antimikroba menunjukkan bahwa ekstrak etil asetat dari daun dan batang memiliki aktivitas penghambatan tertinggi terhadap S. aureus, ekstrak etil asetat dari akar dan batang memiliki aktivitas penghambatan tertinggi terhadap E. coli, dan ekstrak etil asetat dari daun memilikiaktivitas penghambatan tertinggi terhadap C. albicans. Ekstrak etil asetat dari rimpangmemiliki aktivitas antioksidan tertinggi diantara ekstrak lainnya.
STUDI KIMIA DAN FARMAKOLOGI TANAMAN KUNYIT (Curcuma longa L) SEBAGAI TUMBUHAN OBAT SERBAGUNA Simanjuntak, Partomuan
JURNAL ILMU PERTANIAN "AGRIUM" Vol 17, No 2 (2012)
Publisher : JURNAL ILMU PERTANIAN "AGRIUM"

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.671 KB)

Abstract

Abstract                Some reports indicated a great variety of pharmacological activities of kunyit, Curcuma longa  L. which exhibit anti-inflamatory, anti virus, anti bacteria, antioxidant, nematocidal activities, etc. Curcuminoids are major compounds in Curcuma longa  L responsible for the biological activity. This paper compiles an overview of those pharmacological activities and chemical compositions of the kunyit, Curcuma longa establishing its importance in health.Keywords: chemistry, pharmacology, Curcuma longa L, medicinal plants
PRODUKSI ASAM LEMAK OLEAT OLEH MIKROBA ENDOFIT Sporodiobolus salimonicolor DARI TUMBUHAN KINA (Cinchona pubescens Vahl.) Mumpuni, Esti; ., Amalia; Parwati, Titi; Simanjuntak, Partomuan
Alchemy Jurnal Penelitian Kimia Vol 3, No 2 (2004)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

Fermentasi mikroba endofit dari tannaman kina (Cinchona pubescens Vahl.) dalam medium Phoma telah dilakukan. Hasil fermentasi selama 8 hari diekstraksi dengan diklormetan diperoleh asam lemak yaitu asam oleat dalam bentuk cis. Hasil ini dipertegas oleh spectra RMI (proton dan karbon) dan spektramassa (GC-MS).
UJI TOKSISITAS AKUT EKSTRAK AIR TANAMAN SARANG SEMUT (MYRMECODIA PENDANS) TERHADAP HISTOLOGI ORGAN HATI MENCIT Soeksmanto, Arif; Simanjuntak, Partomuan; Subroto, Muhammad Ahkam
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.325 KB) | DOI: 10.31258/jnat.12.2.152-155

Abstract

erbal products in powder or capsule forms made of sarang semut plant have been widely distributed in market.This plant is believed to treat several diseases, such as cancer, gout, liver, stroke, heart, hemorrhoid, back pain,allergy, and as tonicum to increase sexual desire. Information on the plants was still limited on plant distribution,ecology, ethnobotany, and taxonomy, without research on toxicity and clinical aspect. Considering that sarangsemut products has been distributed at market and limited scientific publication of the plant, hence acute toxicitytest of sarang semut extract should be conducted. The results showed that dose of 375 mg/kg bw caused liverdegeneration on day-5 and normal on day-19. Whereas dose of 3750 mg/kg bw caused cell necrosis up to day-12,degeneration still apparent on day-26.
JI BIOAKTIVITAS SENYAWA GLIKOSIDA DARI BIJI KEBEN (BARRINGTONIA ASIATICA L. KURZ) Bustanussalam, Bustanussalam; Simanjuntak, Partomuan
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.273 KB) | DOI: 10.31258/jnat.12.1.9-14

Abstract

The fruits or seeds of Keben (Barringtonia asiatica L. Kurz) were used traditionally for fish poisons, to curvestomach and headeach. The aim of this research was to isolate and identify bioactive compound of n-butanolfraction of active compound against Artemia salina Leach larvae. Isolation and purification of n-butanol fractionwere carried out by column chomatography (SiO2, CHCl3-MeOH) and high pressure liquid chromatography (RP,MeOH). The result of purification was than tested using BSLT methode. The test showed that, BABU-2.4 had thehighest activity with LC 50 value was 30.19 ppm. BABU-2.4 was identified with FT-IR spectrophotometer and NMR(proton and carbon) spectrophotometer as derivate of glicoside.
Co-Authors . Sukarno Abdul Malik Ahmad Sopian, Ahmad Al Qisthi, Farhana Hanida Amalia . Anwar, Yelfi Apriani, Elsa Fitria Arif Soeksmanto Aziz, Zulhelmi Bambang W. Nugroho, Bambang W. Batan, Alfrida Binawati Ginting Bustanussalam ., Bustanussalam Bustanussalam Bustanussalam, Bustanussalam Cyntia Cyntia, Cyntia Daniel Daniel Darmawan, ahmad Dian Malini Otovina, Dian Malini Djihan Ryn Pratiwi, Djihan Ryn DJOKO PRIJONO E.G. Said, E.G. Edi Susanto Edward J Dompeipen, Edward J Eko Mugiyanto, Eko Elvina Dhiaul Iftitah Enny Sudarmonowati ERIS SEPTIANA Esti Mumpuni, Esti Fanny Fanny Fauzy Rachman Gianny, Demitra Harmastini Sukiman, Harmastini Haryo, Haryo Hendig Winarno HERGATIKA, HARUMI Herman Suryadi Hindra Rahmawati Hirotaka Shibuya, Hirotaka izzati, Fauzia Nurul K. Ohashi, K. K. Prana, Titik KARTININGSIH, KARTININGSIH KAWAZOE, KAZUYOSHI KITAMURA, CHINAMI Kusharyoto, Wien Lamek Marpaung, Lamek Lekatompessy, Sylvia J.R. Lenny, Lisya Evy LOGAWA, EMELIA DEVI Lohita Sari, Bina Lohita Sari, Bina Lubis, Misri Yanty M. Rahayuningsih MAEHARA, SHOJI Mahayoga, Gede Adhi Majid, Nur Cholis MARIA BINTANG Mastura, Mastura Mastura, Mastura Mohamad Rafi Muhammad Ahkam Subroto Muhammad Fitrah, Muhammad Muhammad Saifur Rohman Muhammad Walid Murniaty Simorangkir Nampiah Sukarno Nancy Dewi Yuliana Nasution, M Pandapotan Nasution, Pandapotan Nasution, Pandapotan Ngadiman Ngadiman NISA RACHMANIA MUBARIK Noviani, Vini OHASHI, KOZUYOSHI Putra, Andrianopsyah Mas Jaya Putra, Ari Permana Rachman, Fauzy Rachmat, Judhi Rahmaniar Rahmaniar, Rahmaniar Rahmawati, Siti Irma Retno Murwani Ribu Surbakti, Ribu Risma Marisi Tambunan, Risma Marisi rizky maulida amalia hanif, rizky maulida amalia Rudi Kartika, Rudi Rumella Simarmata, Rumella Rurianti, Wandesta Rurianti, Wandesta Rutdianti, Rutdianti Rutdianti, Rutdianti Saifur Rohman Santoso, Ivan Sari Setianingsih, Sari SARONOM SILABAN Setyningrum, Eka Damayanti Setyningrum, Eka Damayanti Shinta Puspitasari Shirly Kumala Siswa Setyahadi Sri Hartati Srikandace, Yoice sudarmo sudarmo Sukiman, Harmastini I. Sulastri, Lilik Suwarno, Tisno Suwarno, Tisno Swasono R. Tamat Syamsudin Abdillah, Syamsudin Thauresia, Shelly Titi Parwati, Titi Titik K. Prana, Titik K. Tiwit Widowati, Tiwit Tonel Barus, Tonel Wiwi Winarti Yadi, Yadi Yatri Hapsari, Yatri Zuhelmi Aziz