Sihono Sihono
Center for Application of Isotope and Radiation Technology, National Nuclear Energy Agency Jl. Lebak Bulus Raya, Pasar Jumat, Jakarta, Indonesia

Published : 8 Documents
Articles

Found 8 Documents
Search

Application of Mutation Techniques in Sorghum Breeding for Improved Drought Tolerance Human, S; Sihono, Sihono; Parno, Parno
Atom Indonesia Vol 32, No 1 (2006): January 2006
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.152 KB) | DOI: 10.17146/aij.2006.116

Abstract

Sorghum is not native to Indonesia and has not yet as popular as other cereal crops. This crop has a big potential to be grown and cultivated owing to its wide adaptability and high productivity. Genetic variability of this crop is still low, thus, plant breeding program is required to support sorghum development in the country. The objective is to develop superior genotypes to improve sorghum production and quality, as food, animal feed or for industry. Research on sorghum improvement through induced mutations has been conducted at the Center for the Application of Isotope and Radiation Technology, National Nuclear Energy Agency (BATAN). Durra variety was used as parental material in the breeding program. Induced mutation was made by Gamma irradiation on seed treatments. The optimal radiation dose was to be around 300-500 Gy. Through selection processes and direct screening for drought tolerance, a number of ten putative mutant lines were obtained. In dry season, the mutant lines B-68, B-72, B-95 and B-100 produced grain yield of 4.55, 4.50, 4.20 and 4.62 t/ha, respectively. These yields were significantly higher than the original parent Durra (3.50 t/ha) and the control check varieties UPCA (2.68 t/ha) and Higari (3.75 t/ha). These promising mutant lines might be of useful for further sorghum research and development in Indonesia
Development of Sorghum Tolerant to Acid Soil Using Induced Mutation with Gamma Irradiation Human, S.; Trikoesoemaningtyas, Trikoesoemaningtyas; Sihono, Sihono; Sungkono, Sungkono
Atom Indonesia Vol 36, No 1 (2010): April 2010
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.302 KB) | DOI: 10.17146/aij.2010.6

Abstract

Water scarcity still becomes a problem in some dryland agricultural areas in Indonesia. Development of dryland farming system may be focused on crops that are required less water such as sorghum. Sorghum is a cereal crop that is usually grown under hot and dry condition and it is ideal for Indonesia. Sorghum is a good source of food, animal feed and raw material for ethanol. Indonesia is currently looking for alternative renewable energy resources and sorghum is regarded as one of the promising source of bioethanol as bioenergy. Unfortunately, most agricultural land in western part of the country particularly in Sumatera and Kalimantan is dryland and dominated by acid soil. The main constraint of crop production in acid soil is deficiency and Al toxicity. Therefore, development of sorghum cultivation in dryland farming system requires a variety which is tolerant to such conditions. Sorghum breeding for acid soil tolerance had been conducted at PATIR-BATAN by using induced mutations with gamma irradiation. The breeding objective was to search for sorghum genotypes tolerant to acid soil condition and with regard to sorghum use for bioethanol production. A number of 66 breeding materials, including the mutants, had been screened for acid soil tolerance on land with soil pH of 4.2 and 39% Al saturation in Lampung Province. Ten sorghum genotypes had been identified as high yielding in the acid soil condition. The mutant lines GH-ZB-41-07, YT30-39-07, B-76 and B-92 had grain yield higher (>4.5 t/ha) than the control plants (Durra, Mandau and Numbu). Sorghum mutants ZH30-29-07, ZH30-30-07 and ZH30-35-07 were promising for grain-base bioethanol production with ethanol yield exceeded 2,000 l/ha. Meanwhile, the sweet sorghum mutants ZH30-35-07, ZH30-30-07 and ZH30-29-07 had brix content of 11.59, 11.95 and 10.50%, respectively. These mutant lines are promising to be developed further in sorghum breeding since they are highly tolerant to acid soils. Received: 20 November 2009; Revised: 20 April 2010; Accepted: 21 April 2010
Stability Test For Sorghum Mutant Lines Derived From Induced Mutations with Gamma-Ray Irradiation Human, S.; Andreani, S.; Sihono, Sihono; Indriatama, W.M.
Atom Indonesia Vol 37, No 3 (2011): December 2011
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.03 KB) | DOI: 10.17146/aij.2011.76

Abstract

Sorghum breeding program had been conducted at the Center for the Application of Isotopes and Radiation Technology, BATAN. Plant genetic variability was increased through induced mutations using gamma-ray irradiation. Through selection process in successive generations, some promising mutant lines had been identified to have good agronomic characteristics with high grain yield. These breeding lines were tested in multi location trials and information of the genotypic stability was obtained to meet the requirements for officially varietal release by the Ministry of Agriculture. A total of 11 sorghum lines and varieties consisting of 8 mutant lines derived from induced mutations (B-100, B-95, B-92, B-83, B-76, B-75, B-69 and Zh-30) and 3 control varieties (Durra, UPCA-S1 and Mandau) were included in the experiment. All materials were grown in 10 agro-ecologically different locations namely Gunungkidul, Bantul, Citayam, Garut, Lampung, Bogor, Anyer, Karawaci, Cianjur and Subang. In each location, the local adaptability test was conducted by randomized block design with 3 replications. Data of grain yield was used for evaluating genotypic stability using AMMI approach. Results revealed that sorghum mutation breeding had generated 3 mutant lines (B-100, B-76 and Zh-30) exhibiting grain yield significantly higher than the control varieties. These mutant lines were genetically stable in all locations so that they would be recommended for official release as new sorghum varieties to the Ministry of Agriculture.Received: 20 November 2010; Revised: 01 August 2011; Accepted: 05 August 2011
RANCANG BANGUN KENDALI ON/OFF LAMPU PENERANGAN DENGAN TEKNOLOGI WIRELESS ZIGBEE SEBAGAI PENUNJANGPRATIKUM MIKROKONTROLER Sihono, Sihono
Orbith Vol 12, No 2 (2016): Juli 2016
Publisher : Orbith

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penerangan ruang merupakan kebutuhan utama yang sangat diperlukan pada setiap  tempat atau ruang baik untuk rumah tangga, perkantoran, tempat-tempat usaha, industri dan tempat- tempat lain. Pada penelitian ini akan dilakukan pembuatan alat kendali lampu penerangan ruang jarak jauh menggunakan teknologi nirkabel dengan protokol Zigbee, sehingga kendali lampu penerangan menjadi lebih mudahdan nyaman.Pembuatan alat kendali lampu nirkabel terdiri dari dua unit yaitu unit kendali yang terdapat saklar kendali ON/OFF lampu dan Unit beban yang berfungsi menterjemahkan informasi data digital menjadi keluaran nyala/padam lampu penerangan. Pada penelitian ini digunakan mikrokontroler AVRATMega 328 sebagai pusat kendali dan modul Zigbee DRF 1604 sebagai penghubung komunikasi data antara unit kendali dan unit beban. Hasil penelitian menunjukan bahwa data digital dapat dikirimkan melalui modul Zigbee sehingga perintah dari unit kendali dapat digunakan untuk memberi perintah nyala atau padam pada lampu penerangan. Pada penelitian ini juga dibuat program untuk merealisasikankendali lampu menggunakan bahasa C menggunakan program IDE Codevision yang merupakan bahasa pemrograman mikrokontroler yang banyak digunakan saat ini.
Alat Peraga Kendali Pemanas Udara Berbasis Arduino Uno Sebagai Penunjang Praktikum Laboratorium Kendali Politeknik Negeri Semarang [A Demonstration Tool of Arduino Based Air Heater Controller to Support Control Laboratory of Politeknik Negeri Semarang] Supriyo, Bambang; Kuntardjo, Samuel Beta; Sihono, Sihono
-
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of this research is to develop and test the demonstration tool of Arduino based air heater controller with Proportional Integral Derivative (PID) based control methods for the temperature range between 50ºC and 70ºC.   Arduino Uno was programmed using C-language to do control tasks and to transfer control data serially to computer via USB port. The selection of the initial PID  parameters are determined using the combination of Relay Feedback method and Ziegler-Nichols  formula.  The control methods were focused on proportional (P), proportional derivative (PD) dan Proportional Integral Derivative (PID) with the performance criteria based on overshoot  dan steady state error.  The experimental results show that the PID controllers give best output responses in terms of zero steady state errors, while P and PD controllers still result in steady state errors of about 3ºC. In addition, P controllers still give about 2,5ºC-4ºC fluctuative output values below set points, while the PD and PID have very small fluctuative values and even very close to zero. This laboratory demonstration tool has shown very good PID based controller performance, so it is feasible to be used as a supporting demonstration tool for control system laboratory in Politeknik Negeri Semarang or even other universities.
Evaluasi In Vitro Silase Sinambung Sorgum Varietas Samurai 2 yang Mengandung Probiotic BIOS K2 dalam Cairan Rumen Kerbau Imanda, Shafa; Effendi, Yunus; Sihono, Sihono; Sugoro, Irawan
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 12, No 1 (2016): Juni 2016
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.403 KB) | DOI: 10.17146/jair.2016.12.1.3193

Abstract

Evaluasi In Vitro Silase Sinambung Sorgum Varietas Samurai 2 yang Mengandung Probiotik BIOS K2 dalam Cairan Rumen Kerbau. Kebutuhan pakan hijauan ternak ruminansia dapat ditingkatkan kualitasnya dengan pembuatan silase. Salah satu teknik silase yang dikembangkan adalah silase sinambung, yaitu suatu teknologi modifikasi pembuatan silase dengan waktu fermentasi yang lebih singkat akibat pemberian bibit silase pada saat awal pembuatannya. Peningkatan kualitas silase dapat dilakukan dengan menambah suplemen berupa probiotik seperti BIOS K2. Tujuan dari penelitian ini adalah untuk mengevaluasi pakan silase sinambung hijauan sorghum varietas Samurai 2 yang mengandung probiotik BIOS K2. Evaluasi pakan dilakukan dengan metode in vitro Hohenheim gas test menggunakan inokulum cairan rumen dari kerbau berfistula yang diinkubasi selama 24 jam pada suhu 390C. Perlakuan terdiri dari pakan A (silase sorgum 21 hari), B (silase sinambung sorghum 3 hari), dan C (silase sinambung sorgum 7 hari). Parameter yang diuji adalah konsentrasi amonia, volatile fatty acids (VFA), sintesis protein mikroba (bakteri dan protozoa), degradasi bahan organik (%DBO), konsentrasi gas karbondioksida (CO2) dan metana (CH4). Data hasil perlakuan dianalisis dengan menggunakan rancangan acak lengkap. Hasil pengujian menunjukkan bahwa adanya pengaruh yang signifikan perlakuan pakan terhadap konsentrasi amonia, konsentrasi VFA, sintesis protein mikroba dan %DBO, sedangkan untuk konsentrasi gas CO2 dan CH4 tidak ada pengaruh. Perlakuan pakan C menghasilkan konsentrasi amonia, VFA dan sintesis protein bakteri berturut-turut sebesar 0,44 mg/ml; 0,89 mg/ml; dan 5,18 mg/ml/jam, lebih tinggi dibandingkan dengan perlakuan A dan B. %DBO tertinggi terjadi pada perlakuan A sebesar 47,30%, sedangkan B dan C sebesar 25,18 dan 37,15%. Sintesis protein mikroba protozoa tertinggi terjadi pada perlakuan A dan B sebesar 2,62 mg/ml/jam, sedangkan perlakuan C sebesar 2,55 mg/ml/jam. Disimpulkan dari percobaan ini bahwa pakan silase sinambung sorgum varitetas Samurai 2 dengan lama inkubasi selama 7 hari (C) memiliki kualitas yang lebih baik dibandingkan dengan silase sorgum yang diinkubasi 21 hari (A).
Evaluasi Jerami Sorgum Varietas Samurai 2 Hasil Iradiasi Gamma secara In Sacco Wahyono, Teguh; Apriliani, Widia; Muawanah, Anna; Sihono, Sihono
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 13, No 2 (2017): Desember 2017
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.513 KB) | DOI: 10.17146/jair.2017.13.2.3527

Abstract

Studi in sacco dilakukan untuk mengevaluasi pengaruh iradiasi gamma terhadap degradasi dan produk fermentasi rumen dari substrat jerami sorgum varietas Samurai 2. Dosis iradiasi yang digunakan sebesar 0, 100 dan 150 kGy bersumber dari cobalt-60. Metode yang digunakan adalah evaluasi secara in sacco dengan titik pengambilan parameter pada jam ke-0, 12, 24, 48 dan 72. Variabel yang diamati adalah degradasi Bahan Kering (BK), karakteristik degradasi BK, degradasi Bahan Organik (BO), karakteristik degradasi BO dan produk fermentasi rumen. Produk fermentasi rumen yang diamati meliputi kondisi pH, konsentrasi amoniak (NH3) dan produksi volatile fatty acid (VFA) total. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan tiga perlakuan dan lima ulangan. Hasil penelitian menunjukkan bahwa pada periode inkubasi ke-72 jam, dosis iradiasi 150 kGy mampu meningkatkan degradasi BK sebesar 21,66% dibandingkan kontrol dan 12,09% dibandingkan dosis 100 kGy (P<0,05). Perlakuan iradiasi gamma dapat meningkatkan nilai degradasi maksimum (a+b) BK pada jerami sorgum varietas Samurai 2 (P<0,05). Perlakuan iradiasi gamma dosis 150 kGy juga mempengaruhi karakteristik degradasi BO yaitu peningkatan parameter degradasi efektif (DE) pada nilai k 0,02 (P<0,05). Perlakuan iradiasi gamma dapat meningkatkan kondisi pH dan konsentrasi NH3 (P<0,05). Kesimpulan dari penelitian ini adalah bahwa Iradiasi gamma dosis 100 dan 150 kGy mampu meningkatkan degradasi BK dan BO. Dosis radiasi 150 kGy merupakan dosis terbaik untuk meningkatkan degradasi BK maksimum (a+b).
Metabolite Profiles and Antioxidant Activity of Caulerpa racemosa with Different Handlings sihono, Sihono; Tarman, Kustiariyah; Madduppa, Hawis; Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.988 KB) | DOI: 10.15578/squalen.v13i3.355

Abstract

 Metabolite profiles and antioxidant activity of Caulerpa racemosa extract with different handlings were investigated. Three different handlings during transportation were applied, namely samples chilled with ice, stored in liquid nitrogen and soaked in seawater. The different handling significantly affected the yield of ethanolic crude extracts and inorganic fractions but insignificantly to organic fractions. Different handlings resulted in differences of major fractions of C. racemosa extracts. Major fractions of the sample that was handled with chilling temperature contained low polar fractions (K10, K11, K12, and K13), while seawater handling extract contained very polar (K1, K2 and K3), polar (K6, K7, and K8) and low polar (K13) fractions. The extract of the sample handled in liquid nitrogen contained balanced fractions. Chilling temperature handling produced highest antioxidant activity (IC50 below 2,000 ppm) in ethanolic extract of C. racemosa. Keywords: antioxidant activity, Caulerpa racemosa, ethanolic extract,handlings, IC50