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HORIZONTAL DISTRIBUTION OF DINOFLAGELLATE CYSTS IN SURFACE SEDIMENTS OF JAKARTA BAY: SOME PRELIMINARY RESULTS Sidharta, Boy Rahardjo; Panggabean, M. G. Lily; Mizushima, Koichiro
Marine Research in Indonesia Vol 33, No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5042.978 KB) | DOI: 10.14203/mri.v33i1.509

Abstract

Ten surface sediment samples were collected from Jakarta Bay to study the horizontal distribution of dinoflagellate resting cysts in this area. Overall results had shown unique species composition and diversity of dinoflagellate cyst assemblages. However, dinoflagellate cysts found in this preliminary research were sparse and relatively low in term of species number and concentrations. Twenty cyst morphophites were identified in this research, within which ten cysts belong to autotrophic and another ten belong to heterotrophic species. Protoperidinium cysts were the most diversified group, predominating in almost the sampling locations. The cysts identified were generally characterized by species belonged to three orders namely Gonyaulacales, Gymnodiniales, and Peridiniales. Only one dinoflagellate cyst found that was belonged to the toxic and harmful algal bloom (HAB) member species, i.e. Gymnodinium catenatum.
POSSIBLE OCCURRENCE OF TOXIC AND HARMFUL PHYTOPLANKTON IN LOMBOK BAY, LOMBOK, INDONESIA Sidharta, Boy Rahardjo; Ahyadi, Hilman
Marine Research in Indonesia Vol 32, No 2 (2007)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7237.004 KB) | DOI: 10.14203/mri.v32i2.455

Abstract

Harmful Algal Bloom (HAB) incidences in Indonesian waters were increasingly occurred from time to time. Extensive and continous studies in this field are needed to be done in more areas in the country. This objective of this present survey were to determine the occurrence of potential toxic and harmful marine microalgae in Lombok, to reveal the diversity of marine microalgae found in the area, and to give some information on the occurrence of HAB phytoplankton in Lombok island. Plankton samples were taken from six stations in Lembar bay, Lombok on February, 2007. This survey found 23 marine microalgae species and two of those were potentially harmful and toxic, namely Dinophysis caudata and Gymnodinium catenatum. Four species, such as Ceratium spp, Dinophysis miles, Prorocentrum gracile, and P micans, were noted to be harmful though so far no report on adverse effect caused by these microalgae in the area. Diatom Chaetoceros spp were the most abundant phytoplankton in almost all of the sampling areas and followed by Ceratium furca and Protoperidinium sp.
Pelatihan Tentang Pengenalan, Pemeriksaan, dan Penjaminan Mutu Bahan Obat Tradisional (BOT) Bagi Guru Biologi SMA Daerah Istimewa Yogyakarta (Training on Determination, Identification, and Quality Control of Traditional Medicine Ingredients to Biology High School Teachers in Yogyakarta Special Province) Sidharta, Boy Rahardjo; Mursyanti, Exsyupransia; Atmodjo, P. Kianto; To’bungan, Nelsiani; Arsiningtyas, Ines Septi
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 2, No 2 (2018): Vol 2, No 2 (2018)
Publisher : Yogyakarta State University

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Abstract

AbstrakKegiatan Pengabdian kepada Masyarakat dengan topik “Pelatihan tentang Pengenalan, Pemeriksaan, dan Penjaminan Mutu Bahan Obat Tradisional (BOT) bagi Guru Biologi SMA Daerah Istimewa Yogyakarta” dilakukan mengingat banyaknya kasus keracunan akibat konsumsi obat tradisional. Guru Biologi SMA menjadi sasaran pelatihan karena telah memiliki latar belakang keilmuan dan keterampilan yang memadai serta sering menghadapi pertanyaan orangtua murid dan masyarakat perihal pemanfaatan BOT. Pelatihan diharapkan memberikan solusi terhadap permasalahan di atas dengan menerapkan teknologi tepat guna yang sederhana, sehingga dapat memberikan pemahaman tentang BOT berkualitas berdasarkan Cara Pembuatan Obat Tradisional yang Baik (CPOTB). BOT yang dipilih yaitu sambiloto (Andrographis paniculata), mahkota dewa (Phaleria macrocarpa), dan pule (Alstonia scholaris) karena banyak digunakan dalam pengobatan diabetes mellitus. Peserta diberikan keterampilan mengenali, mengidentifikasi, dan melakukan pemeriksaan mutu secara sederhana, namun ilmiah. Peserta menyatakan mampu menerapkan keterampilan yang diperoleh dan bersedia mengikuti pelatihan lanjutan di masa mendatang. Kata kunci: Pengenalan, Pemeriksaan, Penjaminan Mutu, Bahan Obat Tradisional  AbstractCommunity Service Activity with the topic “Training on Determination, Identification, and Quality Control of Traditional Medicine Ingredients to Biology High School Teachers in Yogyakarta Special Province” was done due to the increase of traditional medicine intoxications. Biology High School teachers were targeted as the participants, because they had scientific background and skills related to the problem. The activity was done to give better solution to the problem using simple and appropriate technology, hence it can give knowledge on high quality of traditional medicine based on Good Production of Traditional Medicine. Traditional medicines utilised were sambiloto (Andrographis paniculata), mahkota dewa (Phaleria macrocarpa), and pule (Alstonia scholaris), since these traditional medicines were mostly practiced to cure diabetes. Participants were also given skills on determination, identification, and quality control. Participants stated that they were able to apply the skills obtained and were ready to be included in the continual training in the future. Key words: Determination, Identification, Quality Control, Traditional Medicine
THE CURRENT STATUS OF RESEARCH ON HARMFUL ALGAL BLOOM (HAB) IN INDONESIA Sidharta, Boy Rahardjo
JOURNAL OF COASTAL DEVELOPMENT Vol 8, No 2 (2005): Volume 8, Number 2, Year 2005
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Harmful Algal Bloom (HAB) is a natural phenomenon, however its incident increases both in term of cases and areas. When HAB outbreaks occur it will usually damage the environment and create economic losses. Environmental damage and economic losses are caused by the harmful aspects of the HAB organisms due to both of environmental alterations and toxin productions. In Indonesian seas, HAB has become more frequent and spread through out the country since 1970s. But there are still lacks of: number of researcher and research, funding support, awareness, and integrated national agenda with regard to HAB in Indonesia. In contrast, worldwide research and researchers, funding, awareness, and national agenda have become common and more advance. Hence, there are some opportunities for Indonesian researchers on HAB to: join (international) research projects, gain research funding, experience advance training, and pursue scholarships (for Masters and PhDs degree) from institutions abroad.
Inventarisasi Ikan Hias Pantai Gunungkidul, Daerah Istimewa Yogyakarta: Sebuah Kajian Awal Sidharta, Boy Rahardjo; Probosunu, Namastra ; Suwarman, Suwarman
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.477 KB) | DOI: 10.24002/biota.v16i1.68

Abstract

Kajian “Inventarisasi Ikan Hias di Pantai Gunungkidul, Daerah Istimewa Yogyakarta (DIY)” ini diharapkan dapat memberikan data yang lebih akurat dan tepat tentang keberadaan ikan hias di kawasan ini. Kawasan kajian berdasar survei kawasan/lingkungan menetapkan delapan (8) pantai di Kabupaten Gunungkidul sebagai lokasi kajian. Dari delapan pantai tersebut didapat sebanyak 67 jenis ikan hias. Temuan ini seyogianya segera ditindaklanjuti oleh pihak berwenang dalam bentuk penetapan rencana strategis (renstra) yang terkait dengan sumber daya ikan hias, meliputi antara lain: rencana konservasi, pemintakatan (zonasi), pengelolaan, pengembangan, dan pemanfaatan secara berkelanjutan.
AKTIVITAS ANTIBAKTERI EKSTRAK KELOPAK BUAH DENGEN (Dillenia serrata Thunb.) TERHADAP Escherichia coli DAN Staphylococcus aureus Sidharta, Boy Rahardjo
Scripta Biologica Articles in Press
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

AbstRactDillenia serrata (Dengen) is an endemic plant from Sulawesi and it has never been known for its benefit, especially as antibacterial herbs. Unripe Dengen’s fruit is enveloped by sepals, thus it is indicated to have antibacterial activity as a protection of fruit from destructive bacteria. This research aimed to determine the optimum inhibitory of Dengen fruit sepals extracts to Escherichia coli and Staphylococcus aureus. Fruit sepals were extracted with two solvents, which are polar and non-polar, namely methanol and n-hexane. The extraction was done by maceration. Compounds from methanol and n-hexane extracts were qualitatively measured using phytochemical test, such as alkaloid, flavonoid, tannin, triterpenoid/steroid, and saponin. Methanol and n-hexane extracts were divided into three concentrations as treatments for antibacterial test namely 30, 60, and 90 % using disk diffusion method with five replications. The result of phytochemical test showed Dengen fruit sepals contained alkaloids, flavonoids, tannins, triterpenoids/steroids and saponins. The extracts showed optimum inhibitory effect to E. coli which was obtained at 90 % of methanol extract with 0.495 cm inhibitory zone diameter. While, the optimum inhibitory effect to S. aureus was obtained at 90 % of n-hexane extract with 0.54 cm of inhibitory zone diameter. Key words: Dengen fruit sepals, antibacterial, maceration, phytochemical, disk diffusion method
Toxicity of Bioactive Compound from Endophytic Fungi Isolated from Red Ginger (Zingiber officinale var. rubrum) Utilizing Brine Shrimp Lethality Assay Prasetyo, Angga; Sidharta, Boy Rahardjo; Hartini, Yustina Sri; Mursyanti, Exsyupransia
Biogenesis: Jurnal Ilmiah Biologi Vol 7, No 1 (2019)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i1.6000

Abstract

Red ginger (Zingiber officinale var. rubrum) has been proven to show anticancer activity. Direct use bioactive compound from red ginger has many obstacles such as large amount of red ginger’s rhizome needed, limitation of planting area, and very long time of harvesting. Utilization of endophytic fungi from red ginger’s rhizome could be an alternative to the problems. The aims of this study were to determine bioactive compound produced by endophytic fungi and toxicity activity based on LC50. Endophytic fungi were isolated from red ginger and were identified macroscopically and microscopically. The bioactive compounds were extracted using ethanol 96%. Flavonoid test was done qualitatively, bioactive compounds were analyzed by Thin Layer Chromatography (TLC), and the toxicity test was done using Brine Shrimp Lethality Assay (BSLA). The present research found two endophytic fungi isolated from red ginger rhizome. Isolate 1 was similar to Mucor sp. and isolate 2 was similar to Trichoderma sp. Phytochemical test revealed bioactive compound extracted from the isolates were contained flavonoid. TLC analysis did not detect quercetin from the bioactive compound extracted from the isolates. LC50 values of the bioactive compound from the isolates were 2.300 and 1.747 µg/ml, respectively. The toxicological results suggest that both isolates produce non-toxic compound to Artemia salina.
SEAGRESS COVERAGE AND ECOSYSTEM CONDITION AT THE COASTAL AREA OF MADASANGER, JELENGA AND MALUK, WEST SUMBAWA Poedjirahajoe, Erny; Mahayani, Ni Putu Diana; Sidharta, Boy Rahardjo; Salamuddin, Muhamad
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 1 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.648 KB) | DOI: 10.29244/jitkt.v5i1.7744

Abstract

The increase of temperature might affect the distribution and reproduction of seagrass. This research aims to determine the seagrass bed coverage and the ecosystem condition. Three line transects were established perpendicular to the coastal line with the distance of 50-100 m, or up to the border of the intertidal area. In each transect, sampling points were determined with a distance of 10-20 m. At the sampling points, a plot of 50 cm x 50 cm was established to measure the coverage percentage of seagrass vegetation. The seagrass species were also observed and recorded along the line transects. The percentage of seagrass coverage was measured using a method from Saito and Atobe (1994). The results showed that the coastal area of Jelenga has the highest percentage of seagrass coverage (>60%, healthy) among other coastal areas. This may be caused by the characteristic of Jelenga coast which was relatively calm, few visitors, low water turbidity, and high light penetration. While, other transects have percentage coverage of less than 60% (less healthy). There was one transect on Maluk coast which has coverage percentage of less than 29% (lack of seagrass species). The small coverage percentage on Maluk coast can be caused by the high number of visitors and high activity of fishing boats around the coast which results in high turbidity. Keywords: coverage, ecosystem condition, seagrass bed, west Sumbawa.
Dengan Rumput Laut, Membangun Negara (Kajian Buku) Sidharta, Boy Rahardjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.606 KB) | DOI: 10.24002/biota.v10i2.2850

Abstract

Rumput laut (seaweed) adalah biota laut yang mungkin paling dikenal manusia sejak lampau (setelah ikan, tentunya). Manfaat rumput laut bagi manusia juga sangat bervariasi, mulai dari sumber makanan hingga obat-obatan. Di dunia moderen sekarang ini, rumput laut juga semakin dikenal berkat hasil-hasil penelitian ilmiah yang dilakukan manusia terhadap biota ini.
Karakteristik dan Efektivitas Salep Madu Klanceng dari Lebah Trigona sp. Sebagai Antibakteri dan Penyembuh Luka Sayat Cahyadi, Martin Aristo; Sidharta, Boy Rahardjo; To'bungan, Nelsiani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 4, No 3 (2019): October 2019
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.248 KB) | DOI: 10.24002/biota.v4i3.2520

Abstract

Penggunaan antibiotik secara terus menerus dapat menimbulkan resistensi pada bakteri. Salah satu bahan alami yang dapat menjadi alternatif pengobatan adalah madu klanceng. Penelitian ini bertujuan untuk mengetahui sediaan salep madu klanceng yang optimal dan efeknya terhadap penyembuhan luka dan infeksi yang disebabkan oleh bakteri. Tahapan penelitian ini terdiri dari uji fitokimia madu, pembuatan salep, uji sediaan salep, uji kemurnian bakteri, uji zona hambat, dan uji bioassay pada kelinci. Madu klanceng yang diuji mengandung senyawa alkaloid. Variasi salep madu dibuat dengan perbandingan basis salep dan madu adalah 1:1, 1:2, 1:5, dan 5:1. Hasil salep berbentuk semi padat, berwarna  kuning, pH sekitar 4,8, belum homogen, memiliki daya lekat sekitar 265,4 detik, dan daya sebar sebesar 1,76 cm. Salep madu mampu menghambat bakteri S. aureus dan P. aeruginosa dengan diameter sebesar 1,2 mm. Salep madu ini juga memberikan efek menyembuhkan luka hingga 0,3 cm.