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PENGARUH JAMUR Gliocladium sp. DAN BAKTERI Pseudomonas fluorencens DALAM MENEKAN PERKEMBANGAN PENYAKIT LAYU FUSARIUM PADA TANAMAN PISANG MAS (Musa Paradisiaca L.) HASIL KULTUR INVITRO

Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto Vol 17, No 2 (2015): AGRITECH
Publisher : Universitas Muhammadiyah Purwokerto

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Abstract

The objective of the study is to determine the potence of natural agent, a mmushroom of Gliocladium sp. and bacterium Peudomonas fluorencens in resisting against the withering disease (Fusarium Oxysporum f.sp. Cubense) and their effect to the growth of the in-vitrocultured banana plant (Musa paradisiaca L). Thiswas conducted in the experimental farm of Agriculture Faculty, University of Muhammadiyah Purwokerto, in the period of June to December 2013.This research is a single experiment using Randomized Completely Block Design. The treatment was the administration of Gliocladium sp. In three different doses of 10 g/polybag (G1),20 g/ polybag (G2), 30 g/ polybag (G3), and the giving of Pseudomonas fluorencens inthree different dosage of10 ml/l water/ polybag (PF1),20 ml/l water/polybag (PF2) and 30 ml/l water/polybag(PF3) and one control group of no treatment (K0).Based on the result of data analysis, it is proved that the treatment of natural agents of Gliocladiumsp and Pseudomonas fluorencenshas induced the plants resistance against the withering disease of FusariumOxysporumf.sp. Cubense in the banana, as it is indicated bythe increase of phenol compounds, i.e. glychoseda, saponin, and thanin. However, the treatment has no significant effect on the plant growth either on their leaves or their stalk diameter.

PENGARUH STERILAN DAN WAKTU PERENDAMAN PADA EKSPLAN DAUN KENCUR ( Kaemferia galanga L) UNTUK MENINGKATKAN KEBERHASILAN KULTUR KALUS

Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto Vol 12, No 1 (2010): AGRITECH
Publisher : Universitas Muhammadiyah Purwokerto

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Abstract

Penelitian ini bertujuan mempelajari pengaruh senyawa kimia Bayclin (Natrium hipoklorid/NaClO) dan alkohol 70% terhadap penurunan kontaminasi eksplan daun kencur, mencari pengaruh waktu perendaman senyawa kimia sterilan terhadap pertumbuhan eksplan daun kencur serta mengetahui pengaruh interaksi antara senyawa kimia sterilan dan waktu perendaman terhadap peroleh kultur kalus kencur yang bebas kontaminasi. Penelitian ini dilaksanakan pada bulan Oktober 2009 sampai dengan bulan April 2010 di Laboratorium Kultur Jaringan Universitas Muhammadiyah Purwokerto. Rancangan yang digunakan adalah Rancanmgan Acak Lengkap (RAL) dengan 3 ulangan. Variabel pengamatan meliputi : Persentase kontaminasi, persentase eksplan yang tumbuh, waktu pertama kontaminasi muncul, dan sumber kontaminan (Bakteri/jamur). Hasil penelitian menunjukkan bahwa Penggunaan senyawa kimia bayclin (Natrium hipoklorid/NaClO) 20%dan alcohol 70% mampu mengurangi kontaminasi baik eksternal maupun internal yang disebabkan oleh jamur maupun bakteri. Waktu perendaman eksplan dalam senyawa kimia sterilan dengan lama waktu perendaman berkisar 5-10 menit mampu menurunkan kontaminasi antara 35-56 % dalam penelitian ini. Kombinasi penggunaan senyawa kimia bayclin 20% selama 10 menit dan alcohol 70% selama 10 menit mampu menurunkan kontaminasi pada eksplan berkisar 42%.

AKLIMATISASI ANGGREK DENDROBIUM

SAINTEKS Vol 6, No 1 (2010): Jurnal Sainteks Volume VI No 1 Maret 2010
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH PURWOKERTO

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Abstract

This project shows how Dendrobium orchid acclimatization training can develop the skill of the orchid traders of Baturaden district on acclimatization technology, increase the income, and decrease the number of unemployment. The training was conducted towards 10 (ten) participants of PPAB group, and was continued by mentoring and monitoring until they acquired the skill of Dendrobium orchid acclimatization technology. The result proves that (1) the skill of Baturaden orchid traders on acclimatization technology increases, shown by the life percentage of seedling and compote plants that reaches 89.5%; (2) the average income of those traders increases in amount of Rp 4,094,167, or approximately Rp 409,417 per trader; (3) the number of unemployment decreases due to the increasing of employment number in amount of 5 (five) people, consisting of 3 (three) gardeners and 2 (two) florists. This sort of technique was shown to be an effective way of increasing Baturaden orchid traders’ skill on acclimatization technology and their income as well as the number of employment. Key words : Dendrobium orchid, acclimatization, skill           

Kajian Efektifitas Penggunaan Agensia Hayati Trichoderma Sp Untuk Mengendalikan Penyakit Layu Fussarium Pada Tanaman Bawang Merah Diluar Musim

Proceeding Seminar LPPM UMP Tahun 2014 2014: Proceeding Seminar Hasil Penelitian LPPM 2014, 6 September 2014
Publisher : Proceeding Seminar LPPM UMP Tahun 2014

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Abstract

Penelitian dengan judul " Kajian Efektifitas Penggunaan Agensia Hayati Trichoderma Sp Untuk Mengendalikan Penyakit Layu Fussarium Pada Tanaman Bawang Merah Diluar Musim " bertujuan untuk Mengetahui pengaruh agensia hayati Thrichoderma terhadap penekanan serangan jamur Fussarium penyebab penyakit layu pada  tanaman bawang merah serta Pengaruh agensia hayati Thrichoderma terhadap pertumbuhan tanaman bawang merah yang ditanam di luar musim. Penelitian dilakukan pada bulan oktober 2013 sampai dengan Maret 2014, bertempat di lahan percobaan Fakultas Pertanian UMP. Rancangan yang digunakan adalah Rancangan Acak Kelompok (Random Complete Block Design) dan perlakuan diuji terdiri dari 2 faktor perlakuan yaitu faktor pertama adalah jenis agensia hayati Trichoderma yang terdiri dari dua jenis yaitu T. harzianum (T1) dan T. viridae (T2), faktor kedua adalah dosis agensia hayati antagonis Trichoderma dengan taraf  perlakuan sebagai berikut : (D.1) dosis 20 g / lubang tanam, (D.2) dosis 30 g/lubang tanam; (D.3) dosis 40 g/lubang tanam.  Sehingga diperoleh 6 kombinasi perlakuan, ditambah satu perlakuan  tanpa agensia hayati ( kontrol), semuanya disusun secara faktorial dengan tiga ulangan. Data yang diperoleh kemudian dianalisis dengan analisis varian pada taraf 5% jika ada beda nyata maka dilakukan uji lanjut dengan uji Beda Nyata terkecil (BNT) pada taraf 5%. Perlakuan jenis dan dosis  agensia hayati Trichoderma terbukti berpengaruh terhadap penekanan perkembangan patogen Fussarium penyebab penyakit layu pada tanaman  bawang merah selama penelitian. Perlakuan agensis hayati Trichoderma dengan berbagai dosis berpengaruh nyata terhadap parametar jumlah daun dan jumlah umbi, namun tidak berpengaruh nyata terhadap tinggi tanaman dan bobot umbi. Perlakuan Trichoderma viridae pada kisaran dosis 40 g/ lubang tanam memberikan pengaruh terbaik terhadap pertumbuhan tanaman bawang merah selama penelitian. Kata kunci : Trichoderma, Fussarium, bawang merah

Efektifitas Solarisasi Tanah Terhadap Penekanan Perkembangan Jamur Fusarium Pada Lahan Tanaman Pisang Yang Terinfeksi

Proceeding Seminar LPPM UMP Tahun 2014 2014: Proceeding Seminar Nasional LPPM 2014, 20 Desember 2014
Publisher : Proceeding Seminar LPPM UMP Tahun 2014

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Abstract

Penelitian ini bertujuan untuk mengetahui efektifitas perlakuan solarisasi terhadap penekanan perkembangan jamur Fusarium pada  lahan tanaman pisang mas yang terinfeksi di Kecamatan Baturaden, Kabupaten Banyumas.  Penelitian dilaksanakan dilahan endemi penyakit layu Fussarium yang berlokasi di Desa Pamijen, Kecamatan Baturraden, Kabupaten Banyumas. Dengan  ketinggian tempat 175-200 m diatas permukaan laut. Rancangan yang digunakan adalah Rancangan Acak Terbagi dengan faktor perlakuan tunggal yaitu  perlakuan solarisasi yang terdiri dari perlakuan solarisasi dengan plastik transparn dan tanpa solarisasi. Hasil penelitian menunjukkan bahwa Perlakuan solarisasi tanah yang diberikan dimungkinkan berpengaruh dalam penghambatan perkembangan penyakit layu Fussarium pada bibit tanaman pisang mas selama penelitian, terbukti belum munculnya gejala serangan penyakit layu Fusarium pada tanaman pisang  hingga akhir pengamatan.   Perlakuan solarisasi menyebabkan terjadinya peningkatan suhu tanah hingga 45,8 oC dengan kisaran  suhu permukan tanah pada waktu pengamatan pukul 12.00 yaitu berkisar antara  39,5 oC – 45,8 oC dengan lama perlakuan selama 8 minggu. Sedangkan rerata suhu harian tertinggi mencapai 35,45 oC dengan kisaran rerata suhu harian pada permukaan tanah antara 32,13 oC – 35,45 oC, terbukti mampu meningkatkan suhu permukan tanah hingga 8,8 oC dibandingkan tanpa perlakun solarisasi dan berdampak pada penurunan jumlah populasi Fussarium di permukaan tanah hingga mencapai 53,61 %, sedangkan tanpa solarisasi penuruan populasi Fussarium sebesar 22,33 %. Key word : Solarisasi, tanaman pisang mas , lahan terinfeksi Fusarium

UPAYA ALIH TEKNOLOGI PEMBUATAN VIRGIN COCONUT OIL DI DESA KLAPAGADING KECAMATAN WANGON

Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto Vol 10, No 2 (2008): AGRITECH
Publisher : Universitas Muhammadiyah Purwokerto

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Abstract

Kegiatan ini bertujuan untuk memberikan pengetahuan dan ketrampilan kepada ibu rumah tangga dan remaja putri mengenai pembuatan Virgin Coconut Oil. Pelaksanaan kegiatan secara keseluruhan berlangsung selama 8 (delapan) bulan. Rangkaian kegiatan yang dilakukan terdiri dari (1) orientasi lokasi, (2) persiapan bahan, alat, dan materi, (3) kegiatan pelatihan, (4) pengamatan hasil pelatihan, dan (5) evaluasi terhadap pelatihan. Berdasarkan pada hasil evaluasi dan pembahasan, maka dapat disimpulkan bahwa ibu-ibu dan remaja putri di Desa Klapagading, Kecamatan Wangon sudah mulai menyadari dan mengerti pentingnya peranan pengembangan agroindustri khususnya minyak VCO sebagai sarana penunjang kesehatan dan menambah pendapatan keluarga. Selain itu peserta mengetahui peluang dan tantangan agroindustri VCO dengan benar dalam upaya meningkatkan ketrampilan dan pendapatan dari bidang pertanian. Di sisi lain mereka juga bertambah pengetahuannya tentang teknologi pembuatan VCO pada skala rumah tangga secara optimal.

Synthesis of Nanosilica from Padas Stone to Reduce The Total Organic Carbon of Palm Oil Waste

Jurnal ILMU DASAR Vol 20 No 1 (2019)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Padas stone is one of the natural mineral containing 67.5% of SiO2 compounds. In this research, synthesis of nanosilica was carried out by sol-gel method asistanced by 2.45 GHz microwave radiations with low (10%), medium (50%) and high (100%) power at 30 and 60 minutes of contact times. It was analyzed by XRD, BET, FTIR spectrophotometry and SEM. The synthesized silica was then used as absorbent for total organic carbon (TOC) of palm oil waste. The results show that the synthesized silica was a mixture of cristobalite and quartz type minerals according to ICDD No. 00-003-0271 and 01-083-287. The surface area and silicon dioxide contains are 12.174 m2/g and 76.325% for silica without microwave assistance (SiO2-TPMW); 12.796 m2/gand 86.385% for silica with 30 minutes on 10% microwave assistance(SiO2 -MW 10A); 12.735 m2/gand 86.254% for silica with 60 minutes on 10% microwave assistance (MW 10B); 13,659 m2/gand 87.211% for MW 50A; 13,583 m2/g and 86.684% for MW 50B;7.883 m2/gand 57.527% for MW 100A; also 8.752 m2/g and 37.725% for MW 100B, respectively. The use of silica as an absorbent of TOC shows the effectiveness of 62.89% (TPMW); 63.68% (MW 10A); 62.96% (MW 10B); 65.25% (MW 50A); 64.61% (MW 50B); 62.37% (MW 100A) and 61.18% (MW 100B) from the initial TOC of 1520 mg/L. Keywords: synthesis, nanosilica, padas stone, total organic carbon, oil waste.

CHARACTERISTICS OF Chlorella sp BIOMASS IMMOBILIZED ON CHITOSAN (Chlo-Kit) FOR ADSORPTION OF CHROMIUM(III) SOLUTION

ALCHEMY Jurnal Penelitian Kimia Vol 11, No 1 (2015): Alchemy jurnal penelitian kimia
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

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Abstract

Properties of biosorbent prepared from biomass of Chlorella sp immobilized on chitosan (Chlo-Kit) have been studied for adsorption of Chromium(III). Chlo-Kit adsorbent prepared by following steps: dissolving chitosan to get hydrogel form, immobilization of biomasson chitosan hydrogel, beads formation and crosslinkingthe adsorbent beads using epichlorohydrin reagent.Characterization using FT-IR spectrophotometry  and SEM showed that mechanism of biomass immobilization on chitosan involves electrostatic interactions between the functional groups of such both materials. Biomass immobilized on chitosan (Chlo-Kit) showed the higher stability in acid medium than un-immobilized biomass. Adsorption of Cr(III) metal ion on Chlo-Kit occured at optimum pH of 4-5 and followed well the Langmuir adsorption isotherm model. The adsorption capacity of Cr(III) on Chlo-Kit was 68.965 mg/g, about 91% higher than that on Chlorella biomass

Cadmium Adsorption on Chitosan/Chlorella Biomass Sorbent Prepared by Ionic-Imprinting Technique

Indonesian Journal of Chemistry Vol 15, No 2 (2015)
Publisher : Universitas Gadjah Mada

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Abstract

Ionic-Imprinted Chitosan/Chlorella biomass sorbent (IICCb) has been synthesized for selective adsorption of Cd(II) adsorption. IICCb was prepared by coating Cd(II)-complexed-chitosan hydrogel on the biomass surface followed by cross-linking procedure. Cd(II)-imprinting ions were then eluted using Na2EDTA solution to provide a specific template for binding sites of Cd(II). Batch adsorption was carried out as function of initial pH, contact time, and initial concentration of the Cd(II) solution. Result showed that the optimum adsorption of Cd(II) was found at pH 4-6. Study of pseudo-second order kinetic showed that the adsorption of Cd(II) on IICCb went faster than that on Ionic-Imprinted Chitosan (IIC) or Non-Imprinted Ionic Chitosan/Chlorella sorbent (NIICCb). The maximum Cd(II) adsorption capacity as obtained from Langmuir adsorption isotherm was found to be 53.76 mg/g on IICCb, that was comparatively higher than that on IIC (44.44 mg/g) or on NIICCb (51.02 mg/g) adsorbent.

DETERMINATION OF pH EFFECT AND CAPACITY OF HEAVY METALS ADSORPTION BY WATER HYACINTH (Eichhornia crassipes) BIOMASS

Indonesian Journal of Chemistry Vol 6, No 1 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

Effect of pH and determination of adsorption capacity of Cu(II), Ni(II) and Pb(II) heavy metal ions on adsorbent prepared from Eichhornia crassipes (eceng gondok) biomass has been investigated. The influence of media acidity on the adsorption characteristics was carried out by determining ions adsorbed at various pH in the range of 2-10, while an adsorption isotherm model of Langmuir was used to estimate the capacity of adsorption. Results showed that Cu(II) was optimally adsorbed at the range pH of 5-6, Ni(II) at 2-4, while Pb(II) reached an optimum adsorption at pH 2-3. The adsorption data of Cu(II), Ni(II) and Pb(II) for the adsorbent folowed quite well Langmuir isotherm model, confirmed that such chemisorptions involved on that process. The ions adsorption capacities (am) were 27.47, 16.69, and 15.04 mg/g for Pb(II), Cu(II), and Ni(II), respectively.   Keywords: adsorption, heavy metal, Eichhornia crassipes, pH, capacity