Muhamad Nurhalim Shahib
Department of Biochemistry, Faculty of Medicine, Universitas Padjadjaran

Published : 4 Documents
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Integrating Basic Sciences into Global Health by Implementing the Translational Research

International Journal of Integrated Health Sciences VOL 1, NO 1, July (2013)
Publisher : International Journal of Integrated Health Sciences

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Abstract

An emphasize on herbal medicine in cancer therapy is also included as an example of translational research implementation. A translational research in Faculty of Medicine, Universitas Padjajaran, has been conducted to develop a novel genomic approach (herbal genomic) on herbal crude extracts. This study focuses on the effects of the extracts on specific gene expressions. In this aspect, the concept of molecular targets is slightly different from pharmaceutical approaches  that aim to find compounds that interact with a specific molecule or macromolecule with known function. In this research strategy, the herbal genomic approaches involve screening of herbal extracts that affect particular gene expression related to cell responses rather than a single proteins. The determination of herbal concentration is based on differential gene expression before and after treatment which is analyzed by real time PCR. The results are being compared to the existing small molecule (active molecule) on the gene expressions. All these academic activities require biomedical research workforce to implement the translational research which, in the Faculty of Medicine, Universitas Padjadjaran, is fulfilled by involving the postgraduate students. New crude extract formulation plus specific gene expression profile is a concept required for developing chemotherapeutic agent in Indonesia. Hopefully, this review can support increased cooperation between hospital and academic institutions in implementing translational research in Indonesia.    DOI: 10.15850/ijihs.v1n1.86

NR2B Gene Expression Change in Wistar Rat Practicing Aerobic Exercise Comparing to Soybean (Glycine max) or Phyllanthus niruri Intakes and Soybean and Phyllanthus niruri Composition Intake

International Journal of Integrated Health Sciences VOL 3, NO 2, September (2015)
Publisher : International Journal of Integrated Health Sciences

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Abstract

Objective: The objective of this study is to examine the relation between nutrition and brain memory. Qualified human resource is the most valuable national asset. Adequate nutrition and regular exercise provide higher achievement to create healthy and smart generations.Methods: The method applied in this study was experimental laboratory. This study was conducted on Juni 2011–Juli 2012 at the Biomedical and Biochemistry laboratory, Faculty of Medicine, Universitas Padjadjaran. The subjects were 8 weeks approximately 200–250 grams, male Wistar rats. The examination involved 56 male Wistar rats divided into 8 groups with different treatments. The groups which received exercise treatment were also given Glycine max and Phyllanthus niruri intakes. Results: NR2B gene expression changes can be described as the following results: (1) without practicing exercise (3,8) and after exercises (4,6); (2) Glycine max intake minus exercise (2,86) and Glycine max intake and exercises (3,17); (3) Phyllanthus niruri intake minus exercises (4,7) and Phyllanthus niruri intake and exercises (4,9); and (4) Glycine max and Phyllanthus niruri composition intake minus exercises (3,14) and Glycine max and Phyllanthus niruri composition intake and exercises (4,83).Conclusions: This study determines that exercises and Phyllanthus niruri intake enhance NR2B gene expressions. Glycine max inhibits the NR2B gene expressions. Glycine max and Phyllanthus niruri composition intake and either with or without practicing exercises enhance NR2B gene expressions. Therefore, practicing exercises and Phyllanthus niruri intake might cause brain cell apoptosis while Glycine max intake inhibits brain cell apoptosis. Keywords: Exercise, gene expressions, Glycin max, NR2B, Phyllanthus niruri DOI: 10.15850/ijihs.v3n2.582

Integrating Basic Sciences into Global Health by Implementing the Translational Research

International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Original Source | Check in Google Scholar

Abstract

An emphasize on herbal medicine in cancer therapy is also included as an example of translational research implementation. A translational research in Faculty of Medicine, Universitas Padjajaran, has been conducted to develop a novel genomic approach (herbal genomic) on herbal crude extracts. This study focuses on the effects of the extracts on specific gene expressions. In this aspect, the concept of molecular targets is slightly different from pharmaceutical approaches  that aim to find compounds that interact with a specific molecule or macromolecule with known function. In this research strategy, the herbal genomic approaches involve screening of herbal extracts that affect particular gene expression related to cell responses rather than a single proteins. The determination of herbal concentration is based on differential gene expression before and after treatment which is analyzed by real time PCR. The results are being compared to the existing small molecule (active molecule) on the gene expressions. All these academic activities require biomedical research workforce to implement the translational research which, in the Faculty of Medicine, Universitas Padjadjaran, is fulfilled by involving the postgraduate students. New crude extract formulation plus specific gene expression profile is a concept required for developing chemotherapeutic agent in Indonesia. Hopefully, this review can support increased cooperation between hospital and academic institutions in implementing translational research in Indonesia.    DOI: 10.15850/ijihs.v1n1.86

NR2B Gene Expression Change in Wistar Rat Practicing Aerobic Exercise Comparing to Soybean (Glycine max) or Phyllanthus niruri Intakes and Soybean and Phyllanthus niruri Composition Intake

International Journal of Integrated Health Sciences Vol 3, No 2 (2015)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Original Source | Check in Google Scholar

Abstract

Objective: The objective of this study is to examine the relation between nutrition and brain memory. Qualified human resource is the most valuable national asset. Adequate nutrition and regular exercise provide higher achievement to create healthy and smart generations.Methods: The method applied in this study was experimental laboratory. This study was conducted on Juni 2011–Juli 2012 at the Biomedical and Biochemistry laboratory, Faculty of Medicine, Universitas Padjadjaran. The subjects were 8 weeks approximately 200–250 grams, male Wistar rats. The examination involved 56 male Wistar rats divided into 8 groups with different treatments. The groups which received exercise treatment were also given Glycine max and Phyllanthus niruri intakes. Results: NR2B gene expression changes can be described as the following results: (1) without practicing exercise (3,8) and after exercises (4,6); (2) Glycine max intake minus exercise (2,86) and Glycine max intake and exercises (3,17); (3) Phyllanthus niruri intake minus exercises (4,7) and Phyllanthus niruri intake and exercises (4,9); and (4) Glycine max and Phyllanthus niruri composition intake minus exercises (3,14) and Glycine max and Phyllanthus niruri composition intake and exercises (4,83).Conclusions: This study determines that exercises and Phyllanthus niruri intake enhance NR2B gene expressions. Glycine max inhibits the NR2B gene expressions. Glycine max and Phyllanthus niruri composition intake and either with or without practicing exercises enhance NR2B gene expressions. Therefore, practicing exercises and Phyllanthus niruri intake might cause brain cell apoptosis while Glycine max intake inhibits brain cell apoptosis. Keywords: Exercise, gene expressions, Glycin max, NR2B, Phyllanthus niruri DOI: 10.15850/ijihs.v3n2.582