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Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties HASTUTI, DWI; 1, SURANTO; SETYONO, PRABANG
Nusantara Bioscience Vol 1, No 2 (2009): Nusantara Bioscience
Publisher : “Bioscience Community”, School of Graduates, Sebelas Maret University, Surakarta

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Abstract

Hastuti D, Suranto, Setyono P. 2009. Variation of morphology, karyotype and protein band pattern of adenium (Adeniumobesum) varieties. Nusantara Bioscience 1: 78-83. The aim of this research to find out the Adenium obesum variation from six varieties,namely: obesum, cery, red lucas, red fanta , white bigben and harry potter based on morphology, karyotype, as well as protein bandingpattern. The chromosome preparation was made using semi-permanent squash method from the tip of root plant; while protein bandingpattern was made using SDS-PAGE method. Qualitative data included shape and color of the leave and flower described from eachvariety. Data were presented in morphometry and analyzed using ANOVA and then followed by DMRT with 5% of confidence levels,indicated significance difference. Protein banding pattern, the root, stem, leave and all organs were analyzed using Hierarchical ClusterAnalysis method with Average Linkage (between Groups) using SPSS 10.0. The result of research shows that the six A. obesumvarieties have morphological character with no variation of light green to dark green leave, not hairy, smooth leave bone, meanwhile forlight red to dark red flower crown color although some of them are white and the same funnel color, yellow. All varieties of A. obesumhave same number of chromosome, 2n = 22 and shows the difference ranging from 2.56 to 5.13 μm. In the banding pattern formedqualitatively, there is variation among the six varieties.
Alternative supplementary biochemic food for growing up the fresh water lobster (Cherax quadricarinatus) PRIYONO, EDI; PARAMA ASTIRIN, OKID; SETYONO, PRABANG
Nusantara Bioscience Vol 1, No 3 (2009): Nusantara Bioscience
Publisher : “Bioscience Community”, School of Graduates, Sebelas Maret University, Surakarta

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Abstract

Priyono E, Astirin OP, Setyono P. 2009. Alternative supplementary biochemic food for growing up the fresh water lobster(Cherax quadricarinatus). Nusantara Bioscience 1: 123-130. This research denotes to know the influence of biochemic composition tothe rapid grow of fresh water lobster on the stadium of post larva (PL) of 60 within three months. This research used the completerandom planning dealing with 4 treatments and each treatment would get 3 times cycle. The treatments cover, group K tested animalwas treted with 100% mill food containing 30% of protein. Group A is given with food and biochemic food containing 13,34% ofprotein. Group B is the treated with mill food which is mixed with biochemic food containing 10,7% of protein. While group C wastested by treating them with mill food and biochemic food containing 13,58%. After all the above mentioned would be set up within 3:1comparation. The variable of this research were the length of the abdomen, cephalothorax, total length, and the wet weight. The dataanalysis is using ANOVA system on 95% power test completed by of SPSS version 13. The result of the research shows that mentionedtreatments give us the same influence toward the growth of fresh water lobster. The composition of biochemic food with the containingprotein around 10,7%,13.34% and 13,38% has given the same effect to the lobster growth on post larva 60 level. There is strongcorrelation between abdomen and cephalothorax and between the total length and the lobsters weight.
PENGARUH KEPADATAN CACING TANAH TERHADAP EMISI CO2 mesocosm PADA KONVERSI LAHAN HUTAN KE PERTANIAN Dwiastuti, Sri; Sajidan, Sajidan; Suntoro, Suntoro; Setyono, Prabang
Prosiding Seminar Biologi Vol 10, No 1 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Tujuan penelitian ini untuk mengetahui: (1)  pengaruh Sistem Penggunaan Lahan  terhadap kepadatan cacing tanah, (2)  pengaruh  kepadatan cacing tanah terhadap emisi CO2 mesocosm (3) kontribusi kepadatan cacing  terhadap emisi CO2  mesocosm.  Lokasi  penelitian Didaerah Gondangrejo dengan enam Sistem Penggunaan Lahan yaitu: (1) Hutan, (2) Agroforestri Kompleks, (3) Agroforestri Sederhana, (4) Monokultur Jati, (5) Polikultur Jati-Akasia dan (6) Tanaman semusim kacang tanah. Penelitian ini bersifat eksploratif-deskriptif-eksperimental. Inventori cacing tanah dilakukan dengan metode handsorting dengan monolit dari enam Sistim Penggunaan Lahan tersebut diatas. Data penelitian dianalisis secara kuantitatif  menggunakan metode statistik dengan   alat bantu  SPSS 0.16.Dari hasil penelitian dapat disimpulkan bahwa: (1) Sistem Penggunaan lahan berpengaruh signifikan (p<0,05) terhadap kepadatan cacing tanah musim penghujan dan berpengaruh sangat signifikan (sig<0.01) terhadap kepadatan cacing  tanah musim kemarau , (2) Kepadatan cacing tanah berpengaruh sangat signifikan (sig<0,01) terhadap emisi CO2mes vegetatif dan emisi CO2 mesocosm total, (3) Kontribusi kepadatan cacing tanah musim hujan memberikan kontribusi sangat kecil yaitu pada emisi CO2 mes vegetatip 1,8 % sedang pada emisi CO2mes  total 6,3 %, sedang kontribusi kepadatan cacing kemarau memberikan kontribusi lebih besar yaitu pada emisi CO2 mes veg 71,8 % dan pada emisi CO2 mes total 49,7 %    Keyword: cacing tanah,emisi CO2mesocosm.
Estimation of Water Losses Through Evapotranspiration of Water Hyacinth (Eichhornia crassipes) Sasaqi, Dilyan; Pranoto, Pranoto; Setyono, Prabang
Caraka Tani: Journal of Sustainable Agriculture Vol 34, No 1 (2019): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.666 KB) | DOI: 10.20961/carakatani.v34i1.28214

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Batujai Reservoir locates in Batujai Village, Praya Barat, Central Lombok, West Nusa Tenggara. It is the primary source of irrigation water supply for agriculture in Central Lombok District with an area of around 3,235 ha. The problem is the bloom of water hyacinth weed (Eichhornia crassipes), which can cause reservoir water loss through evapotranspiration, affecting the amount of water reservoir available for the dry season. The objective was to identify the area of cover and estimate water loss through water hyacinth evapotranspiration for the period 2013 – 2017. This study used a descriptive method by analysis of secondary data which were meteorological data and landsat-8 satellite imagery. Evapotranspiration analyzes use CROPWAT 8.0, monitoring water hyacinth cover using landsat–8 satellite imagery processed using ENVI 5.3 and ArcGIS 10.4 software. The results show that the spatial distribution of water hyacinth can be detected and mapped accurately with an overall classification accuracy of 84.11% – 97.04% using Landsat 8 data, with a kappa coefficient of 0.80 – 0.96. The area of water hyacinth cover ranges from 38,400 m2 – 2,158,500 m2, with a cover area of more than 20%, causing water loss above 8,000 m3 day-1, which occurred in April 2013, April 2015, April 2016, February 2015, May 2014, May 2016 and July 2016, in those months it was seen that the amount of water loss was greater. Therefore, it is needed to suppress the growth of water hyacinth, in maintaining reservoir water storage capacity to support a systems of sustainable agriculture.
The Vulnerability Level of Groundwater Quality Degradation in The Ecosystem of Sand Dune Landscape on the Southern Coast of Central Java and the Yogyakarta Special Region, Indonesia Setyono, Prabang
International Journal of Advances in Applied Sciences Vol 3, No 2: June 2014
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.11591/ijaas.v3i2.4591

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The sand dune ecosystem phenomenon in the research area has unique and interesting characters. It is  identified that there is an  increase in the construction of the environment in the use of sand dune ecosystem which influence the groundwater quality. Such phenomenon is the manifestation of the existence of spatial dynamics influencing the groundwater degradation vulnerability. This research is aimed at 1) predicting the level of groundwater quality degradation vulnerability based on the sand dune ecosystem spatial dynamics; 2) developing an environmental management model to continuously maintain the availability of groundwater. The research employed survey research and inductive mapping. The aspect of the study included an environmental study with hydromorphological and hydrogeological approach. Analysis and evaluation were based on the multiplication of weight and rate, and were done on landscape units resulting from the map overlay. The research result  indicates  that there are 4 (four) classes of groundwater degradation vulnerability out of the 5 (five) classes of vulnerability determined beforehand. Groundwater degradation vulnerability classes III and IV indicate  problems of disturbances in the groundwater supply system and domestic and agricultural waste pollution. In order to overcome the disturbances in the groundwater supply system, water infiltration well should be constructed, communal domestic water processing should be encouraged, and in order to reduce pollution from the use of agricultural fertilizers, the fertilizers should be blended with clay.
The Association between Risk Factors and Blood Pressure in the Textile Industry Workers Sumardiyono, Sumardiyono; Hartono, Hartono; Probandari, Ari; Setyono, Prabang
Global Medical & Health Communication (GMHC) Vol 5, No 3 (2017)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v5i3.2650

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Noise exposure is often found in the industrial environment that exposed workers at risk for increasing blood pressure. This study aimed at investigating the association between noise level, duration of noise exposure, age, use of earplugs, and body mass index with blood pressure on textile industry workers. An observational study with the cross-sectional design conducted during August to October 2016. The study population was 180 textile industry workers in Surakarta selected by consecutive sampling method. Statistical analysis used was multiple logistic regression. Results showed that variables associated with systolic blood pressure were the use of earplugs (OR=12.7), noise level (OR=7.2), body mass index (OR=5.3), age (OR=4.4) and duration of noise exposure (OR=3.5). Variables associated with diastolic blood pressure were the use of earplugs (OR=6.9), age (OR=6.6), noise level (OR=6.1), body mass index (OR=4.4), and duration of noise exposure (OR=3.1). In clonclusion, the risk factors for blood pressure increased among industrial workers are the use of earplug, noise level, body mass index, age and duration of noise exposure.ASOSIASI ANTARA FAKTOR RISIKO DAN TEKANAN DARAH PADA PEKERJA INDUSTRI TEKSTILPaparan kebisingan yang mengekspos pekerja sering ditemukan di lingkungan industri sehingga berisiko terjadi peningkatan tekanan darah. Penelitian ini bertujuan mengetahui hubungan tingkat kebisingan, durasi paparan kebisingan, usia, penggunaan sumbat telinga, dan indeks massa tubuh dengan tekanan darah pada pekerja industri tekstil. Penelitian ini menggunakan jenis observasional dengan desain cross-sectional yang dilakukan pada bulan Agustus–Oktober 2016. Populasi penelitian adalah pekerja industri tekstil di Surakarta. Terpilih 180 orang pekerja dengan menggunakan metode sampling konsekutif. Analisis statistik yang digunakan adalah regresi logistik berganda. Variabel yang berhubungan dengan tekanan darah sistole adalah penggunaan sumbat telinga (OR=12,7), tingkat kebisingan (OR=7,2), indeks massa tubuh (OR=5,3), usia (OR=4,4), dan durasi paparan kebisingan (OR=3,5). Variabel yang terkait dengan tekanan darah diastole adalah penggunaan sumbat telinga (OR=6,9), usia (OR=6,6), tingkat kebisingan (OR=6,1), indeks massa tubuh (OR=4,4), dan durasi paparan kebisingan (OR=3,1). Simpulan, faktor risiko peningkatan tekanan darah di kalangan pekerja industri tekstil adalah penggunaan sumbat telinga, tingkat kebisingan, indeks massa tubuh, usia, dan durasi paparan kebisingan.
ZONASI DAN KOMPOSISI VEGETASI HUTAN MANGROVE PANTAI CENGKRONG DESA KARANGGANDU KABUPATEN TRENGGALEK PROVINSI JAWA TIMUR Mughofar, Ahmad; Masykuri, Mohammad; Setyono, Prabang
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 8, No 1 (2018): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (838.013 KB) | DOI: 10.29244/jpsl.8.1.77-85

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A research on mangrove forest at coast Cengkrong in the village of Karanggandu aimed at defining zoning and composition of magrove vegetation. The sampling method consists of 3 zoning. Each zone established 3 plots, consists of 10 m x10 m plot size of trees category, 5 m x 5 m of stake category, and 1 m x 1 m of seedling category to repeat in 3 times. Data analysis of mangrove vegetation employed Improtance Value Indeks (IVI). The research results found 12 mangrove species namely Avicennia alba, Bruguiera gymnorrhiza, Bruguiera parviflora, Ceriops decandra, Ceriops tagal, Lumnitczera racemosa, Rhizophora apiculata, Rhizophora mucronata, Sonneratia alba, Sonneratia caseolaris, Xylocarpus granatum Nypa fruticans. Reffering to the the mangrove zonation indicates the zone I or open zone grows Sonneratia alba and it can be found Rhizophora mucronata, Ceriops decandra with salinity of 6 ppt. Zone II or middle zone grows species of Avicennia alba and it also be found Sonneratia alba, Xylocarpus granatum with salinity of 6 ppt. Zone III or zone that is closer to the landward grows species Xylocarpus granatum and also appears Lumnitzera racemosa,Bruguiera parviflora with salinity of 4 ppt. It showed that the formation of mangrove zonation at Coast Cengkrong is still categorized as less stable, due to rehabilitation since 2002 were done without understanding the type of site suitability and natural factors which causes the fruits drop when the tides of sea water, so that the growth is not stable.
PEMETAAN KUALITAS BAKTERIOLOGIS AIR PADA POKMAIR KECAMATAN WATUMALANG Pujiyati, Pujiyati; Setyono, Prabang; Wiryanto, Wiryanto
Media Kesehatan Masyarakat Indonesia Vol 13, No 3: SEPTEMBER 2017
Publisher : Faculty Of Public Health, Hasanuddin University, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30597/mkmi.v13i3.1623

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Penyediaan air secara mandiri oleh masyarakat Watumalang dinilai belum memenuhi syarat kualitas. Kondisi ini dipandang sebagai penyebab terjadinya Kejadian Luar Biasa (KLB ) diare pada beberapa lokasi di Watumalang. Penelitian ini bertujuan mengetahui kualitas bakteriologis pada air konsumsi dan persepsi lingkungan masyarakat serta memetakan hasil pengkajian keduanya di Kecamatan Watumalang. Evaluasi kualitas bakteriologis dilakukan dengan uji koliform melalui metode Most Probable Number (MPN) dari sampel air masyarakat POKMAIR di Kecamatan Watumalang, sedangkan persepsi lingkungan dinilai dari hasil kuisioner responden. Analisis hasil evaluasi kualitas bakteriologis dan persepsi lingkungan dilakukan secara deskriptif. Hasil kedua variabel penelitian selanjutnya ditampilkan dalam format spasial menggunakan software ArcGis. Hasil penelitian menunjukkan bahwa mayoritas air baku tidak memenuhi syarat regulasi (71%) dengan hanya 29% yang memenuhi persyaratan. Hanya satu desa (Limbangan) yang kualitas airnya dianggap layak secara regulasi apabila ditinjau dari kontaminasi koliformnya. Tinjauan perspektif lingkungan menunjukkan mayoritas responden (60%) memiliki persepsi lingkungan yang baik, khusunya pada kesehatan lingkungan.
Keanekaragaman, Zonasi Serta Overlay Persebaran Bentos di Sungai Keyang, Ponorogo, Jawa Timur Pratami, Vivin Alfyana Yulia; Setyono, Prabang; Sunarto, Sunarto
DEPIK Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Vol 7, No 2 (2018): August 2018
Publisher : Faculty of Marine and Fisheries, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.7.2.9881

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Abstrak. Bentos merupakan organisme yang hidup sesil atau menetap di dasar sungai, dan dapat digunakan sebagai indikator pencemaran lingkungan. Penelitian ini bertujuan untuk menentukan keanekaragaman bentos, untuk mengetahui zonasi serta overlay persebaran bentos di Sungai Keyang, Kabupaten Ponorogo, Jawa Timur. Pengambilan sampel dilakukan pada bulan Oktober-November 2017 dengan metode plot/transek, penghitungan, dan analisis laboratorium. Terdapat 3 stasiun pengamatan, masing-masing stasiun terdiri dari 3 transek dan 9 plot. Pengukuran faktor lingkungan meliputi suhu, turbiditas, pH, kandungan bahan organik, DO, BOD, dan tekstur substrat. Hasil dari penelitian diperoleh 7 famili dan 14 spesies bentos. Hasil pengukuran indeks keanekaragaman (H’) yaitu 1,37-2,12 termasuk dalam kategori sedang, memiliki kemerataan yang tinggi (2,12-2,28), dan dominansi rendah (0,13-0,30). Kesimpulan dari penelitian ini adalah keanekaragaman bentos di Sungai Keyang termasuk dalam kategori sedang, zonasi dan overlay persebaran bentos pada bagian tepi dan tengah didominasi oleh Melanoides dan Tarebia, sedangkan pada bagian tergenang didominasi oleh Melanoides dan Cordulegaster.Kata kunci: bentos, keanekaragaman, Sungai Keyang, overlay persebaran, zonasi
Konservasi Mangrove Berbasis TRM (Tanam Rawat Monitoring) untuk Menjaga Sumberdaya Laut di Cengkrong, Trenggalek Paringsih, Novia Citra; Setyono, Prabang; Sunarto, Sunarto
Bioeksperimen: Jurnal Penelitian Biologi Vol 4, No 2: September 2018
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v4i2.6882

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Hutan Mangrove terletak di Kabupaten Trenggalek, Jawa Timur telah mengalami penurunan spesies diakibatkan perilaku konsumtif masyarakat dalam memanfaatkan mangrove. Keberadaan mangrove sangat penting untuk menjaga kesetabilansumberdaya laut khususnya perikanan. Penelitian ini bertujuan untuk mengetahui Indeks Nilai Penting (INP) pada tiga zonasi, melakukan pendekatan kepada masyarakat sekitar terkait partisipasi dalam konservasi mangrove, dan membuatstrategi konservasi hutan mangrove berbasis Tanam Rawat Monitoring. Metode penelitian deskriptif kuantitatif, pengambilan data mangrove random sampling dan pengambilan data wawancara kepada masyarakat sekitar purposive sampling. Analisis vegetasi menggunakan Indeks Nilai Penting, analisis partisipasi masyarakat menggunakan Pressure State Response. Hasil penelitian zonasi A didominasi Sonneratia alba (99,84%) kategori pohon, Sonneratia alba (89,03%) kategori anakanpohon, Rhizophora mucronata (80,74%) kategori semai, zonasi B didominasi Sonneratia alba (120,57%) kategori pohon, Sonnratia alba (57,55%) kategori anakan pohon, Sonneratia alba, Ceriops tagal, Rhizophora apiculata (32,47%)kategori semai, zonasi C didominasi Lumnitzera racemosa (132,40%) kategori pohon, Xylocarpus granatum (113,03%) kategori anakan pohon, Lumnitzera racemosa (60,28%) kategori semai. Pendekatan partisipatif dan kemitraan kepadamasyarakat sekitar melalui Pokmaswas Kejung Samudera berjalan lancar. Kesimpulannya konservasi berbasis Tanam Rawat Monitoringdi kawasan mangrove Cengkrong lebih efektif untuk diterapkan,  konservasi mangrove tersebut diharapkan dapat menjadi role model masyarakat untuk menjaga hutan mangrove dan sumberdaya laut.