Surachmi Setyaningsih
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MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS (MAP) BECOMES A PUBLIC HEALTH CONCERN IN DEVELOPED COUNTRIES WHICH ASSOCIATED WITH CROHN’S DISEASE (CD) IN HUMAN AND JOHNE’S DISEASE (JD) IN RUMINANTS.  SOME RESEARCHERS IN EUROPE, USA, AND AUSTRALIA DETECTED MAP IN THE DAIRY PRODUCTS AND SHOWED THE RELATIONSHIP AMONG MAP, CD, AND JD.  MEANWHILE INDONESIA IMPORTED MILK AND MILK PRODUCTS FROM THOSE COUNTRIES TO COVER NATIONAL DEMAND.  IN THE FUTURE IT WILL BE A POTENTIAL PROBLEM TO NATIONAL D Nugroho, Widagdo Sri; Sudarwanto, Mirnawati; Lukman, Denny Widaya; Setyaningsih, Surachmi; Naim, Rochman; Usleber, Ewald
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn?s disease (CD) in human and Johne?s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy herd and human health.  The aim of this study is to detect MAP in the growing up milk formula.  Fifty samples from five established distributors were taken in Bogor.  Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold?s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer.  Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57.  Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia.   Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR
KEMAMPUAN PRIMER IS900 DAN F57 MENDETEKSI MYCOBACTERIUM AVIUM SUBSPESIES PARATUBERCULOSIS DENGAN PCR KONVENSIONAL Nugroho, Widagdo Sri; Sudarwanto, Mirnawati; Lukman, Denny Widaya; Setyaningsih, Surachmi; Hassan, Abdulwahed Ahmed; Usleber, Ewald
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

Abstract

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