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Kandungan Senyawa Aktif Spirulina platensis yang Ditumbuhkan pada Media Walne dengan Konsentrasi NaNO3 Berbeda Notonegoro, Hartoyo; Setyaningsih, Iriani; Tarman, Kustiariyah
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): Desember 2018
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v13i2.555

Abstract

AbstrakSpirulina platensis merupakan sumber makanan organik  yang mengandung protein tinggi dengan asam amino yang seimbang. Spirulina juga memiliki kandungan senyawa aktif yaitu fikosianin dan flavonoid. Senyawa aktif tersebut pada umumnya memiliki aktivitas yang potential sebagai suplemen dan sediaan bahan aktif pada pangan fungsional. Salah satu faktor yang mempengaruhi kandungan fikosianin dan flavonoid dari mikroalga adalah nutrisi yang digunakan dalam media pertumbuhan sehingga perlu dilakukan kajian mengenai pengaruh komposisi media terhadap kandungan fikosianin dan flavonoid. Penelitian ini bertujuan untuk menentukan pengaruh variasi kandungan NaNO3 pada media pertumbuhan terhadap kandungan fikosianin dan flavonoid S. platensis; serta menentukan konsentrasi NaNO3 terbaik pada media Walne untuk menghasilkan biomassa S. platensis dengan kandungan fikosianin dan flavonoid  tertinggi. Penelitian ini terdiri dari 2 tahapan yaitu kultivasi S. platensis; dan ekstraksi fikosianin, flavonoid, dan komponen aktif lainnya. Kultur S. platensis menggunakan media Walne dengan variasi kandungan NaNO3 yaitu masing-masing sebesar 80, 100 dan 120 g selama 11 hari. Total protein dan nitrogen tertinggi diperoleh pada perlakuan 100 g NaNO3 masing-masing sebesar 44,30% dan 7,09%. Biomassa S. platensis setiap perlakuan NaNO3 mengandung flavonoid, steroid, fenol dan saponin. Konsentrasi dan rendemen fikosianin terbaik diperoleh pada perlakuan 80 g NaNO3 sebesar 1,32 mg/ml dan 32,93%.Total flavonoid ekstrak S. platensis tertinggi diperoleh pada perlakuan 80 g NaNO3 sebesar 16,56%. S. platensis terpilih adalah perlakuan NaNO3 80 g karena menghasilkan kandungan senyawa aktif flavonoid dan fikosianin tertinggi. ncentration. The Active Compounds of  Spirulina  platensis Grown on Walne Media with Different NaNO3 ConcentrationsAbstractSpirulina platensis is a source of organic foods that contain high protein with balanced amino acids. Spirulina also contains active compounds of phycocyanin and flavonoids. The active compound communly to have potential activities as supplement and functional food. One of the factors that affects the content of phycocyanin and flavonoids from microalgae is the nutrients used in growth media. So that it is necessary to study the effect of media composition on the content of phycochyanin and flavonoids. This study aimed to determine the effect of various concentrations of NaNO3 on growth media on the content of flavonoids and phycocyanin and determine the best NaNO3 concentration in Walne media that produce S. platensis biomass with the highest phycocyanin and flavonoid contents. This study was run in two steps that were cultivation of S. platensis and extraction of phycocyanin, flavonoid, and other active components. S. Platensis were cultured in Walne media with various NaNO3 concentrations that were 80, 100 and 120 g for 11 days. The highest total protein and nitrogen were obtained from 100 g NaNO3 treatment which were 44.30% and 7.09% respectively. The biomass of S. platensis for each of NaNO3 treatment contains flavonoids, steroids, phenols and saponins. The best concentration and yield of phycocyanin were obtained from the treatment of 80 g NaNO3 of 1.32 mg/ml and 32.93% respectively. The highest total flavonoids of S. platensis extract were obtained at 80 g NaNO3 treatment of 16.56%. The best treatment to grow S. platensis which produced highest active content of phycocyanin and flavoniud was gained from 80 g NaNO3 medium
KAJIAN KERUSAKAN TABLET HISAP SPIRULINA SELAMA PENYIMPANAN Setyaningsih, Iriani; Trilaksani, Wini; Desniar, .; Masruroh, Emma; Fahleny, Ria; Gentini, Vatin Tri
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.62 KB)

Abstract

Spirulina is a microalgae have a high nutrient content and bioactive compound were good for health. This microalgae can be cultivated indoor or outdoor as needed. One of the applications of Spirulina is for lozenges. The purpose of this study is determine the effect of packaging and shelf life of Spirulina lozenges on the growth of bacteria and fungi. This microalgae can be cultivated indoor or outdoor as needed. One of the applications of Spirulina is for lozenges. Bacteria and fungi still grow on the Spirulina lozenge which packaged using alumunium foil and plastic bottle. However bacteria and fungi total on Spirulina lozenge during storage on 8 weeks are not different.
POTENSI PROBIOTIK BAKTERI ASAM LAKTAT ASAL BEKASAM IKAN NILA Nurnaafi, Astri; Setyaningsih, Iriani; -, Desniar
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.783 KB) | DOI: 10.6066/jtip.2015.26.1.109

Abstract

Bekasam is well known in Indonesia as one of fermented fish product. Several fermented products generate lactic acid bacteria (LAB) which has probiotic potential with beneficial effects on human health. However, In Indonesia, the research on LAB isolated from fermented fish product, including bekasam, is still rarely conducted. The aim of this study was to evaluate the probiotic potential of LAB isolated from bekasam. Two LAB isolates namely NS(5) and NS(6) were selected based on their resistance to gastric pH (pH 2.0), intestinal pH (pH 7.2) and bile salts (0.5% oxgal). Pathogenic test, antimicrobial activity test, characterization and identification of the isolats were also performed respectively. The result showed that NS(5) isolate survived at pH 2.0, pH 7.2 and bile salts (oxgal). It was obtained that NS(5) isolate was non pathogenic bacteria which exhibited antimicrobial activity against Salmonella Typhimurium ATCC 14028 and Escherichia coli. The characterization result showed that NS(5) isolate was Gram-positive bacteria, rod-shaped, non-endospore producer, negative catalase, homofermentative, non motile, having an amilolitik as well as lipolitik activity and able to grow at 30-37°C, NaCl 2-7% dan pH 4.4-9.6. Isolate NS(5) isolate was then identified as Lactobacillus plantarum 1 strain with 99.9% of similarity. Meanwhile, NS(6) isolate was not able to survive in the medium containing bile salts (oxgal), therefore it was not categorized as a probiotic candidate.
PRODUKSI BIODIESEL DARI BIOMASSA Chlamydomonas sp. ICBB 9113 DIKULTIVASI MENGGUNAKAN MEDIA YANG MURAH: EFEKTIFITAS DARI BEBERAPA METODE EKSTRAKSI Patmawati, Patmawati; Ibrahim, Bustami; Setyaningsih, Iriani; Sudadi, Untung
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : LIPI-Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.292 KB)

Abstract

The main challenges to overcome in biodiesel production from microalgae are lower oil yield, as compared to those derived from plant and animal biomass, and expensive culture media. This work was aimed to compare the effectivity of three extraction methods differed in solvent used, e.g. n-hexane (N-hex), ethanol (Eth), and mixture of chloroform-methanol-water (CMW), to extract crude lipid and biodiesel from dry biomass of Chlamydomonas sp. ICBB 9113. This microalgae was cultivated in a cheap culture media using N and P soil fertilizers as nutrient sources. The results showed that, by using N-hex, Eth, and CMW methods, it could be extracted, respectively, 0.06%, 4.51%, and 20.45% crude lipid, and 384.2, 1333.8, and 2430.6 mg/100g biodiesel. The fatty acid profile of the studied microalgae biomass was: C8:0 (0.11%), C10:0 (0.09%), C14:0 (7.70%), 16:0 (1.39%), C18:0 (0.85%), C14:1 (5.12%), C16:1 (7.09%), C18:1 (8.28%), C18:2 (12.80%), and C18:3 (42.57%). Fatty acid characterization showed that Chlamydomonas sp. ICBB 9113 was dominated by C18: 3 and C16:0. Therefore, these microlagae were suitable to be used as raw material for biodiesel production to substitute the conventional fuel.
PENGARUH STARTER BAKTERI ASAM LAKTAT PROBIOTIK TERHADAP PERUBAHAN KIMIAWI DAN MIKROBIOLOGIS RUSIP Batubara, Pebry Aisyah Putri; Desniar, Desniar; Setyaningsih, Iriani
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 1 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.481 KB) | DOI: 10.6066/jtip.2019.30.1.28

Abstract

Rusip is one of the fish fermentation products. Traditional rusip made by Bangka Belitung community do not have a specific standard which leads to the high variety ofquality. One of the efforts to improve the quality of rusip is the addition of lactic acid bacteria (LAB) as a starter culture. This research aims to study the effect of the addition of starter culture combinations on the chemical and microbiological changes of the rusip and toobtain the best combination of LAB starters in rusip fermentation. This research used anchovy fish (Stolephorus sp) and 5% ofLAB starter combination consisting of Lactobacillus plantarum SK(5) and L. plantarum NS(5), L. plantarum NS(5) and L. plantarum NS(9) and L. plantarum NS(9) and L. plantarum SK(5) 5%. In addition, 5% ofsalt (w/w) and 5% of palm sugar (w/w) were added. The fermenta-tions were conducted for 8 days. The research were conducted in three stages: 1) starter culture prepara-tion; 2) raw material (fish) preparation; and 3) application of starter culture during rusip fermentation. The results showed that raw materialscontained a total microbe of 3.0x104 CFU/g, non detectable levels ofLAB, 6.54of pH and 20.40 mg N/100g of total volatile bases (TVB). After fermentation, the rusip contained total LAB of7.64-9.24log CFU/g, 0.86-3.98% of total lactic acid,4.22-6.36 of pH, 0.32-1.39 of free amino acid and 5.34-5.44% of salt.The best rusip was produced bya combination of L. plantarum SK(5) and L. plantarum NS(5) and con-tained12.36 g/100g of total amino acid.
ANTICANCER ACTIVITY OF SPIRULINA CULTIVATED IN WALNE AND ORGANIC MEDIA Sirait, Putriana Sari; Setyaningsih, Iriani; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.088 KB) | DOI: 10.17844/jphpi.v22i1.25876

Abstract

Spirulina is Cyanobacteria containing active components which is potentially showing anticancer activity. The purposes of this study were to determine anticancer activity and selectivity of crude extractsof Spirulina cultured using Walne and organic media, and to detect the apoptosis. The stages of this study included cultivation and harvesting of Spirulina, active components extraction, anticancer test and apoptosisdetection. Anticancer activity was determined using MTT assay. The crude extracts of Spirulina from Walne and organic cultures contained active components of alkaloids, flavonoids, steroids, and saponins. Theseextracts were not toxic to normal breast cells (MCF-12a), but showed cytotoxic activity in breast cancer cells (MCF-7). The crude extract of Spirulina from Walne culture had IC50 value of 36.23 ppm and selectivity index 30.07, while the IC50 of organic culture was 117.78 ppm and selectivity index 7.17. Detection of apoptosis with Hoechst dye 33342 showed the apoptotic activity of Spirulina crude extract against MCF-7 cells.
PENGARUH MEDIA KULTIVASI CHAETOCEROS GRACILIS TERHADAP KANDUNGAN KIMIAWI DAN POTENSI INHIBITOR PROTEASE [EFFECT OF CHAETOCEROS GRACILIS CULTIVATION MEDIA TO THE CHEMICAL CONTENT AND PROTEASE INHIBITOR POTENTIAL] Setyaningsih, Iriani; Nurhayati, Tati; Aremhas, Uzainah
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.533 KB) | DOI: 10.6066/jtip.2013.24.2.222

Abstract

Microalgae produce secondary metabolites with different characteristics for each genus, species or strain. A single species of microalgae can produce several bioactive compounds, including protease inhibitors which can prevent deterioration of fish. In this study, we observed the growth of Chaetoceros gracilis in the media NPSi and NPSi + NaHCO3 and determined the chemical content and the potency of protease inhibitor from Chaetoceros gracilis in both media. The culture was harvested at 8 and 15 days. Screening of protease inhibitor activity was performed by agar diffusion method. Protease inhibitor activity was tested on three pathogenic protease-producing bacteria, namely Staphylococcus aureus, Bacillus cereus and Escherichia coli. The pathogenic bacteria often contaminate foodstuffs. The results showed that media NPSi and NPSi + NaHCO3 affected protein and lipid content of C. gracilis, but the culture age did not affect them. The protein content of C. gracilis cultivated in NPSi media (34.75 and 32.94%) was higher than in NPSi + NaHCO3 media (28.13 and 27.13%), while the lipid content was 16.36 and 18.06, 23.86 and 25.40% respectively. Extracts of C. gracilis grown in NPSi and NPSi+NaHCO3 media had inhibitory activity against the test bacteria. Inhibitory activity against E. coli was greater than S. aureus and B. cereus.
Ekstrasi Senyawa Antibakteri Dari Diatom Chaetoceros gracilis dengan Berbagai Metode Setyaningsih, Iriani; Hardjito, Linawati; Monintja, Daniel R.; Sondita, M. Fedi A.; Bintang, Maria; Lailati, Nispi; Panggabean, Lily
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.24 KB) | DOI: 10.14203/jbi.v5i1.3203

Abstract

ABSTRACTExtraction of Antibacterial Compound from Diatom Chaetoceros gracilis WithDifferent Methods. Diatom is phytoplankton which is commonly found in off shore.Chaetoceros produce antibacterial which inhibit some bacteria. This research was donein 3 steps. At first, Chaetoceros gracilis was cultivated in temperate controlled roomwith lighting 24 hours. The culture was harvested on 14 days, then it was dried andweighted. The second step, biomass was disrupted by different method such as sonicator,glass beads, and undisrupting. Then the biomass was maserated, filtrated, and evaporated.The crude extracts were tested to pathogen bacterial. The third step, the extraction wasconducted using hexana (non polar solvent), ethyl acetate (semi polar solvent), andmethanol (polar solvent). The crude extracts were tested to the pathogenic bacteria.The result showed that the produce cell disruption antibacterial activity by sonicator.The biggest inhibition zone was obtained by hexana but produced lower yield.Key words: Chaetoceros gracilis, growth, extraction, disrupting, antibacteria
The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Chrismadha, Tjandara; Sartika, Diani; Setyaningsih, Iriani; Uju, Uju
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/47

Abstract

A green alga,  Chlorella vulgaris  was grown semi-continuosly at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed  in 10 L glass  bottles provided with aeration  for gas exchange and  mixing. Light  was  obtained  from  440  watt  cool  fluorescent  lamps  placed  at  one  side  of  the  culture.  Room temperature was  26-32C.  Algal  lipid  extraction  was  done  based  on  liquid  phase  separation  of  methanol :chloroform:water  and  the  antioxidant  activity  was  examined  by  means  of  oxidation  inhibitory  in ethanol emulsified  limnoleic  acid.  In  addition,  a  TLC  analysis  was  performed  to  identify  the  antioxidant compouns soluble  in  the  lipid.  The  results  showed  that  harvesting  per iod  had  a  significant  influence  on  the algal lipid  content,  which  were  11.94,  12.96,  and  16.51%  of  the  dry  weight  in  the  culture  with  harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the  harvesting period on the  antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can  be associated   with  the  algal  antioxidant  activity,  which  were  pheophorbide -a,  chlorophyll-b,chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one. Key words: microalgae, Chlorella vulgaris, lipid, antioxidant, harvesting period 
ANTIOXIDANT ACTIVITY OF SELECTED FORMULA SPIRULINA PLATENSIS TROCHES BASED ON PYSICAL CHARACTERISTICS Fahleny, Ria; Trilaksani, Wini; Setyaningsih, Iriani
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 2 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.481 KB) | DOI: 10.29244/jitkt.v6i2.9019

Abstract

ABSTRACT Spirulina contains complete nutrition and bioactive components as a source of antioxidants. The aims of this research were to determine a formula of troche from Spirulina platensis according to the physical characteristic standard of the Indonesian Health Ministry, and to determine the antioxidant activity of the selected troche from Spirulina platensis. This research was conducted in three stages. The first stage was troches formulation. The second stage was troches physical analyses, including weight uniformity, friability, hardnes, and disintegration time. The third stage was to determine the antioxidant activity of the selected formula of troche from Spirulina platensis. There were five formulas of troches i.e., FTS 1, FTS 2, FTS 3, FTS 4, and FTS 5. The troche consisted of Spirulina platensis powder, carrageenan, gelatin, corn flour, sugarleaf, and mint powder. Based on the weight uniformity of the troche, all formulas met the standard. However, according to friability aspect, the best formula was FTS 1 with rate of 1.7%. The hardness of five formula ranged from 0.65 to 2.3 Kp, and none met the standard. The best formula based on disintegration time measurement was FTS 1 (24:47 minutes). Based on the physical characteristics of the troches, the selected formula was FTS 1. The antioxidant activity (IC50 of the selected formula (FTS 1) was 288,68 ppm.   Keywords: antioxidant, troches, physical characteristics of troches, Spirulina platensis