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Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA)

Jurnal Veteriner Vol 13, No 1 (2012)
Publisher : Jurnal Veteriner

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Abstract

Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.

Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER

Jurnal Veteriner Vol 8, No 4 (2007)
Publisher : Jurnal Veteriner

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Abstract

A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.

Perubahan patologis dan gambaran lekosit pada itik yang diinfeksi Pasteurella multocida

Hemera Zoa Vol 77, No 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Enam puluh ekor itik yang berumur lima minggu dipergunakan dalam penelitian ini. ltik dibagi secara acak menjadi dua kelompok yaitu kelompok infeksi dan kontrol.Kelompok pertama diinfeksi dengan Pasteurelhz multocida (Isolat lapangan) secara intramuskular. Itik diambil darahnya dan dinekropsi 1, 2, 4 dan 8 jam setelah infeksi untuk pemeriksaan diferensial lekosit dan perubahan makroskopik.Itik yang diinfeksi mulai menunjukkan lesio empat jam setelah infeksi. Itik yang diinfeksi dan mati menunjukkan gejala septikemi dengan hiperemi umum. Pemeriksaan gambaran lekosit menunjukkan peningkatan jumlah heterofil dan penurunan jumlah limfosit dan monosit 2,4 dan 8 jam setelah infeksi.

Kasus infeksi cacing saluran pencernaan pada ayam hutan hijau (Gallus varius) yang diperiksa di laboratorium patologi unggas FKH_IPB

Hemera Zoa Vol 76, No 2 (1993): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Telah ditemukan kasus inifeksi cacing saluran pencernaan pada ayam hutan hijau (Callus varius) yang diperiksa di Laboratorium Patologi Unggas, Fakultas Kedokteran Hewan lnstitut Pertanian Bogor, periode bulan Agustus 1992 - Januari 1993.Dari 62 ekor ayam hutan hijau yang mati, 26 ekor (41,93%) diantaranya positif terinfeksi cacing setelah dilakukan nekropsi. Dari 26 ekor ayam yang dinyatakan positif terinfeksi, 22 ekor terinfeksi oleh cacing pita (Cestoda), 3 ekor terinfeksi cacing gilik (Nematoda) dan 1 ekor ayam terinfeksi oleh kedua macam cacing tersebut.Hasil pemeriksaan terhadap parasit ini menunjukkan bahwa cacing pita yang menginfeksi ayam adalah Raillietina sp. dan cacing gilik yang ditemukan adalah Ascaridia galli. 

Enteritis necroticans pada ayam broiler akibat infeksi sekunder clostridium perfringeens Tipe A

Hemera Zoa Vol 76, No 1 (1993): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

The experiment was conducted to determine the relationship between conccidiosis vaccination and secondary infection of Clostridium perfringens type-A as a cause of Enteritis Necroticans in broiler chickens.The completely randomized design was used in this experiment. In this study 102 Arbor Acres strain unsexed chicken were randomly assigned to 8 treatment groups with 12 chicks each. Feed and water were given ad libitum.Treatment factors in this experiment were coccidiostat (Salinomycin 6%), coccidiosis vaccine and Clostridium perfringens type-A isolate, and their combinations.The anatomy pathology (AP) and histopathology (HP) figures of the chicken intestines were evaluated.Histopathology results indicated that the relationship between coccidiosis vaccination and secondary infection of Clostridium perfringens type-A was highly significant (p < 0.01) but the anatomy pathology figures showed that the relationship was not significant (p < 0.05). 

Pengujian secara In Vitro Oligosakarida dari Ekstrak Tepung Buah Rumbia (Metroxylon sago Rottb.) sebagai Sumber Prebiotik

Jurnal Agripet Vol 9, No 2 (2009): Volume 9, No. 2, Oktober 2009
Publisher : Agricultural Faculty

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Abstract

In vitro analysis of oligosaccharide from extract rumbia fruit (Metroxylon sago Rottb.) as prebioticABSTRACT. Despite a range of commercially available oligosaccharides there is plenty of room to develop new, functionally enhanced prebiotics. current generation of oligosaccharides was not rationally developed. better understanding of factors determining the prebiotic activity of a particular oligosaccharide. Despite the range of commercially available oligosaccharides mixtures (mainly fructo and galacto-oligosaccharides), very few studies are focused on the mechanisms behind the prebiotic activity of particular oligosaccharides. Probably this lack is due to the unavailability of well characterized oligosaccharide fractions for prebiotic function assessment. The objectives of this research were to asses the ability of lactic acid bacteria in fermentation of oligosaccharide and as prebiotic (in vitro). Material used was oligosaccharide of purified rumbia fruit extract. Analysis of oligosaccharide as prebiotic was conducted in vitro using lactic acid bacteria. The lactic acid bacteria consisted Bifidobacterium bifidum, Bifidobacterium animalis, Lactobacillus bulgaricus and Lactobacillus casei Rhamnosus. The growth media for bacteria was a liquid MRS basic medium where glucose was substituted by oligosaccharide of purified rumbia fruit extract. Incubation was in aerob for Lactobacillus and anaerob for Bifidobacterium in incubator 37oC. The lactic acid bacteria was calculated 24-48 hours during incubation periode. The variables observed were: oligosaccharide component, ability of lactic acid bacteria in fermentation of oligosaccharide, and growth of lactic acid bacteria (Lactobacillus and Bifidobacterium). The result showed that the oligosaccharide component from extract rumbia fruit consisted of: sucrose, stacchiose, and raffinose. The result showed that the oligosaccharide extract rumbia fruit was significantly (P<0.05) the growth of lactic acid bacteria (Lactobacillus and Bifidobacterium) and fermentation of oligosaccharide. It is concluded that oligosaccharide of rumbia fruit extract could be used as prebiotic.

DETEKSI IMUNOHISTOKIMIA ANTIGEN Coxiella burnetii SEBAGAI PENYEBAB Q FEVER PADA SAPI

Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan mendeteksi keberadaan antigen Coxiella burnetii sebagai penyebab Q fever pada organ sapi yang dikumpulkan di rumah potong hewan (RPH) Kota Medan dan Kabupaten Deli Serdang. Pada penelitian ini telah dikumpulkan organ limpa, paru-paru, dan hati dari 162 ekor sapi. Sampel organ tersebut kemudian diperiksa secara imunohistokimia dengan metode streptavidin peroksidase untuk melihat keberadaan antigen Coxiella burnetii menggunakan antibodi poliklonal terhadap Coxiella burnetii. Hasil pemeriksaan imunohistokimia menunjukkan 62/162 (38,3%) sampel sapi imunoreaktif terhadap Coxiella burnetii. Berdasarkan asal pengambilan sampelnya, sebanyak 40/101 (39,6%) sampel sapi yang berasal dari RPH Kota Medan dan 22/61 (36,1%) sampel sapi yang berasal dari RPH di Kabupaten Deli Serdang menunjukkan hasil imunoreaktif. Dilihat dari jenis organnya, dari 162 sampel sapi, antigen Coxiella burnetii dapat dideteksi pada 61 (37,7%) organ limpa, 12 (7,4%) organ paru-paru dan 2 (1,2%) organ hati. Hasil ini menunjukkan telah adanya infeksi Coxiella burnetii pada sapi di Kota Medan dan Kabupaten Deli Serdang.

Profil Kinetik dan Efektivitas Enrofloksasin yang Dikombinasikan dengan BioATP dalam Mengatasi Coxiella burnetii (KINETIC PROFILE AND EFFECTIVITY OF ENROFLOXACINE WITH BIO ADENOSIN TRIPHOSPHATE SUPPLEMENTATION AGAINST COXIELLA BURNETII)

Jurnal Veteriner Vol 14, No 3 (2013)
Publisher : Jurnal Veteriner

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Abstract

Coxiella burnetii belongs to rikettsia group living obligate intracellularly and as the agent of zoonosisQ fever. Enrofloxacine is an antibiotic in quinolon group used to treat infection of C. burnetii in chicken,goat, calve, pig, dog, cat,  and horse. From ruminant practical experience, enrofloxacine if combined withBioATP  can enhance the enrofloxacine activity. Research for the effecivity of enrofloxacine and BioATP totreat C. burnetii has never been carried out. The research was conducted to explore effect of enrofloxacinewith supplementation BioATP against C. burnetii. Enrofloxacine pharmacokinetic study was carried outby using simental beef as an experimental animals. The effectivity of BioATP supplementation onenrofloxacine activity to treat C. burnetii was tested by using Vero cell tissue culture. The results showedthat combination of enrofloxacine and BioATP increased kinetic profile of enrofloxacine in term of onset,duration, pharmacology intensity, and bioavailaibility. Enrofloxacine had activity to treat C. burnetii withvalue of minimal inhibitory concentration (MIC) at 1-2 ppm and value of minimal bactericidal concentrationat 4 ppm. Supplementation of BioATP improved the effectivity of enrofloxacine in treating C. burnetii.

Potency of Medicinal Plants for Eradication of Avian Influenza : In Vitro Test on Vero Cells

Jurnal Sain Veteriner Vol 31, No 1 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Some of medicinal plants indicate their potency as anti-viral such as Sambiloto (Andrographis paniculata Nees), Temu Ireng (Curcuma aeruginosa L.), Beluntas (Pluchea indica L.) Sirih Merah (Piper crocatum) and Adas (Foeniculum vulgare).  Avian Influenza (AI) H5N1 strain viruses used in this study was isolated from field in Cikole area, West Java in July 20th 2007.  To explore the potency of medicinal plants as anti-viral substance, the consecutive assays were performed by virus infection inhibition test in in vitro study using Vero cells.  After the Vero cells were growing confluently, they were treated with sterilized-extract of medicinal plants either in single or combination.  Furthermore, the culture cells were infected with AI H5N1 strain virus, then incubated at 37oC and examined for cytopathic effect (CPE) microscopically.  The result showed that extract of Sambiloto and combination of Sambiloto and Temu Ireng were stronger than others in inhibition of virus attachment and infection to the cells.  The Vero cells still alive up to 3rd day post infection with AI H5N1 virus after treatment with Sambiloto and Temu Ireng.  In conclusion, extract of Sambiloto and Temu Ireng showed their potency as candidate for anti-viral substances that may needed for eradicating AI infection.

The effect of alpha fetoprotein on NF-κB translocation in lipopolysaccharide induced monocyte-derived dendritic cell

Medical Journal of Indonesia Vol 21, No 2 (2012): May
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Background: Alpha fetoprotein (AFP) is a tumor-associated Ag that has a function in both ontogenic and oncogenic growth and its serum level is elevated in patients with hepatocellular carcinoma (HCC). A recent study showed that the immunoregulatory effect of AFP was through impairment of dendritic cell function as antigen presenting cell (APC), a mechanism that is known to hamper efficient antitumor response. However, the underlying intracellular mechanism of action of AFP required elucidation. As an initial step to determine the signaling pathway of AFP, we analyzed whether LPS induced NF-κB translocation occured in AFP-treated monocyte-derived dendritic cell (MDDC), which was induced by lipopolysaccharide (LPS).Methods: Monocytes were cultured in GM-CSF (800 ng/mL) and IL-4 (1000 ng/mL) containing medium and incubated for six days to generate immature MDDCs with or without the presence of AFP. Mature MDDC was generated by stimulation of the immature MDDC with LPS for another 30 minutes. The analysis of NF-κB translocation was measured by fluorescent microscopy.Results: Following activation of MDDC by LPS, the control group showed a marked nuclear staining of NF-κB. However, the AFP-treated group showed negative nuclear staining similar as observed in unactivated MDDC.Conclusion: This study demonstrated that AFP prevented the activation and nuclear translocation of NF-κB and subsequently might cause the impairment of MDDC function as APC. This finding provides a new insight on the role of AFP in the suppression mechanism of anti tumor immune response. (Med J Indones. 2012;21:97-101)Keywords: Alpha fetoprotein, dendritic cell, lipopolysaccharide, NF-κB translocation