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Nintya - Setiari
biologi

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Induksi Kalus Binahong (Basella rubra L.) Secara In Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda

Bioma Vol. 13, No. 1, Tahun 2011
Publisher : Bioma

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Abstract

Binahong (Basella rubra L.) is a plant medicine consisting secondary metabolites which have virtue asmedicines for several diseases that could also be used as coloring agent. The medicine compounds in secondarymetabolites could be extracted from callus. Sucrose is one of the components that build MS (Murashige & Skoog)medium. Sucrose is important in in vitro culture, it functions as carbon and energy source for explant to grow. Thepurposes of this research are to study the effect of sucrose in MS medium towards B. rubra L callus formation andgrowth; to find the optimum sucrose concentration for callus B. rubra L formation; and also to find the fastestinitiation time to produce callus crumb. This research uses Complete Random Design (CRD) single factor method,i.e., sucrose concentration of 0 g/l, 10g/l, 20 g/l, 30 g/l, 40 g/l with five repetitions. The data is analysed withAnalysis of Varian (Anova) and if a real difference is found the analysis is continued with Duncan Multiple RangeTest (DMRT) with significancy level of 95%. The results show that various sucrose concentrations in MS mediuminfluences callus B. rubra L induction. The highest sucrose concentration, i.e. 40 g/l, which was added into MSmedium, could induce the maximum callus wet-weight of 1,69 g and the fastest callus initiation time of 4,8 day.

Induksi Kalus dari Hipokotil Alfalfa (medicago sativa l.) secara in vitro dengan Penambahan Benzyl Amino Purine (BAP) dan α-Naphtalene Acetic Acid (NAA)

Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

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Abstract

Alfalfa (Medicago sativa L.) is useful plant for treatment some diseases such as: cancer, diabetes, lupus, andhepatitis. Propagation of this plant in Indonesia face a problem which has no embryo. One method to propagate thisplant is by tissue culture or micropropagation. Callus induction is first step in micropropagation to produce calluswhich will be regenerated to become planlet. The aims of this research are to induce callogenesis from hipocotyl ofalfalfa with addition Benzyl Amino Purine (BAP) and α Naphtalene Acetic Acid (NAA), and to determine the propercombination of BAP and NAA to produce the optimal callus. The experiment has been conducted by using 12combination of BAP and NAA with Completely Randomized Design (CRD) in 4x3 factorial pattern by 5 replicates.Data were analyzed by ANOVA 95% Degrees of Freedom (DF). If there was significance result, it was followed byDMRT analyzed at 95 % DF. The result showed that combination of BAP and NAA was able to induce callogenesisfrom hipocotyl of alfalfa. The optimal callus was obtained in combination of BAP 0 ppm and NAA 2 ppm.

Eksplorasi Kandungan Klorofil pada beberapa Sayuran Hijau sebagai Alternatif Bahan Dasar Makanan Tambahan

Bioma Vol. 11, No. 1, Tahun 2009
Publisher : Bioma

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Abstract

Chlorophyll is a plant pigment which recently have been popular as food supplement. The source ofchlorophyll consumed now are alfalfa’s leaves algae such as Spirulina and Chlorella.. Chlorophyll content find in allkinds of plant especially vegetables so that have potent to be source of chlorophyll. The aims of this research are toexplore alternative source of chlorophyll aside of Leguminosae, from vegetables such as sweet basil, spinach, waterspinach, cassava leaves, pegagan, and papaya’s leaves and to study the vegetables potent to be food supplement.The experiment has been conducted by extracting 0,1 g each leaves in 10 mL acetone 85%. The extract was filteredwith Whatman paper No. 1, thus record the absorbance by using spektrofotometer UV Vis. Statistical analysis wasused to analyze chlorophyll content is Analysis of Variance (ANOVA) with Randomized Complete Design (RCD) in95% significancy. If the ANOVA showed significantly different, the analysis were continued with Duncan’s test.The result showed that the highest content of chlorophyll-a, b, and total found in papaya’s leaves. However thelowest one was found in sweet basil. Papaya’s leave potential as food supplement.