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Kajian Fisis Radiasi Plasma Terhadap Organ Daun pada Pertumbuhan Awal Tanaman Anggrek Phalaenopsis amabilis [Physics Observation of Plasma Radiation for Leaves to Initial Growth of Phalaenopsis amabilis] Nur, Muhammad; Setiari, Nintya; Azam, Much.; Selawanti, Ika Indah
BERKALA FISIKA Vol 10, No 1 (2007): Berkala Fisika
Publisher : BERKALA FISIKA

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The Phalaenopsis amabilis (”anggrek”) has been irradiated by nitrogen ions for growth  accelereting.Irradiation technique used corona plasma technology that its electrodes configuration was point-to-plan geometry to generate nitrogen ions in air. Corona glow discharge Plasma has been generated by DC Voltage of 3.50 kV and current was 0,23 mA.  Nitrogem ions that were irradiated to Phalaenopsis amabilis (”anggrek”) have been bombarded to  leaf organ during 1 to  7 minutes. We used two types Phalaenopsis amabilis control:  control with fertilizer and control without fertilizer. Growing of Phalaenopsis amabilis  can be accellereted about 90 % compare with control without  fertilizer and 30 % compare with control with fertilzer. The maximum growing of Phalaenopsis amabilis by using plasma technology for time irradiation of 1 or 2 minutes.   Key words  : corona glow discharge plasma, Phalaenopsis amabilis (anggrek), nitrogen, ion electrons,  free radicals.
Kapasitas Penyerapan dan Penyimpanan Air pada Berbagai Ukuran Gel dari Tepung Karaginan untuk Pembuatan Media Tanam Jeloponik Hakim, Muhammad Faisol; Setiari, Nintya; Izzati, Munifatul
JURNAL ANATOMI FISIOLOGI Vol 17, No 1 (2009): Volume XVII, Nomor 1, Maret 2009
Publisher : JURNAL ANATOMI FISIOLOGI

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There is a trend in using gel for hydrophonic. We examine the potency of water absortion and holding capacity from different size of carrageenan gel. This research was conducted from may to july 2006. the size of gel, i.e.: 1 cm3, 2 cm3, 3 cm3 and in powder form were used as independent variables. Where as, water absorbtion and holding capacity act as dependent variables. Collected data were analized by ANOVA. Result indicated that gel size significantly effect water absorbtion and holding capacity. In powder form, water absorbtion and holding capacity is the most optimal, but the gel was easily destroyed. Therefore, the best size if gel that should be used for hydrophonic were 2 cm3 and 3 cm3.
PERTUMBUHAN TUNAS LATERAL TANAMAN NILAM (Pogostemon cablin Benth) SETELAH DILAKUKAN PEMANGKASAN PUCUK PADA RUAS YANG BERBEDA Irawati, Heny; Setiari, Nintya
JURNAL ANATOMI FISIOLOGI Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : JURNAL ANATOMI FISIOLOGI

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Patchouly is one of the plants that produce essential oil, i.e. patchouly oil, which is commonl  used in cosmetic industry. Increasing the biomass of patchouly plants by triggering the growth could be one way to increase the production of pathouly oil. Therefore, the purpose of this research is to study the effect of apical bud cutting towards patchouly growth, and also to understand the optimum location of cutting on the nodus. The research used Complete Random Design method with 5 repetitions. There were 3 locations of cutting (R), i.e. R0 (without cutting), R1 (cutting at the first nodus), R2 (cutting at the second nodus). The observed variables are length and amount of lateral bud, wet weight, and dried weight. The data were then analysed using ANOVA with significancy level of 95%. If there were real different, the analysis were then continued using Duncan test with significancy level of 95%. The results showed that apical bud cutting gives real effect to the variable of length and amount of lateral bud. The untreated plant has the most lateral bud shorter than the treated ones. For the variables of wet and dried weight there were not any significant differents. Generally, cutting at the first nodus (R1) of patchouli plant gives better result than any other treatments.
Pembentukan Akar pada Stek Batang Nilam (Pogostemon cablin Benth.) setelah direndam Iba (Indol Butyric Acid) pada Konsentrasi Berbeda Hasanah, Farida Nur; Setiari, Nintya
JURNAL ANATOMI FISIOLOGI Vol 15, No 2 (2007): Volume XV, Nomor 2, Oktober 2007
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This paper studies the effect of plant growth regulators, i.e., indol butyric acid (IBA), to the root growth in stem cutting technique of P. cablin, and the correct concentration of the IBA to obtain optimum root growth. The research were carried out in Lab Bio Struktur dan Fungsi Tumbuhan, UNDIP, Semarang. Complete Randome Design was used as the research method in which 4 different concentrations, i.e., control 0, 25, 50 and 75 ppm, were observed with parameter of the amount of the root, the length of the root, wetted weight, dried weight, and efficiency. The results show immersing the stem into 25 ppm IBA exhibits the highest root growth efficiency.
Perlakuan Defoliasi untuk Meningkatkan Pembentukan dan Pertumbuhan Cabang Lateral Jarak Pagar (Jatropha curcas) Darmanti, Sri; Setiari, Nintya; Romawati, Tanti Dwi
JURNAL ANATOMI FISIOLOGI Vol 16, No 2 (2008): Vol. XVI, No. 2, Oktober 2008
Publisher : JURNAL ANATOMI FISIOLOGI

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Jatropha is an alternative resource of sustainable energi. Therefore, to support the use of sustainable energi,an increase production of Jatropha seed is important. As Jatropha seeds (fruits) only growth at terminal andaxial bud, therefore the presence of lateral bud is necessary to increase production of Jatropha seed.Formation of lateral bud is affected by the balance between auxin and sitokinin. Defoliation will control thebalance of these two hormone. In this study, defoliation is conducted on seedling at the age of 4 month.Each treatment was replicated    7 times. Variabel were observed and ended after 6 month. Result indicatecthat either defoliation will stimulated the formation and growth of lateral bud.
Produksi dan Konsumsi Oksigen serta Pertumbuhan Ceratophyllum demersum L. pada Kerapatan yang Berbeda dalam Mendukung Potensinya sebagai Bioaerator Hidayat, Muhammad Khusni; Izzati, Munifatul; Setiari, Nintya
JURNAL ANATOMI FISIOLOGI Vol 19, No 2 (2011): Volume XIX, Nomor 2, Oktober 2011
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Aquaculture is one of important economic activity in Indonesia. The main problem in aquaculture is the low water quality such lower oxygen level . Ceratophyllum demersum is one of aquatic plants that is hypothesized capable in increasing oxygen level through photosynthesis. However the growth of C. demersum it self will consume oxygen from the water  through respiration. The aim of this study is to measure oxygen production and consumption by C. demersum at different density level. From this data, we will understand the potency of  C.demersum as bioaerator to supplay oxygen in the water. This experiment was designed using Completed Randomized Designed (CRD). Three density level of C. demersum was apllied as treatment. They were 100g/100L, 200g/100L dan 300g/100L. Each treatment was replicated by 4 times. Results indicated that in density of 300g/100L produced the highest oxygen level ( 1,65 ppm). The highest growth rate of C. demersum was resulted by density level of 200g/100L.
Respon Pertumbuhan dan Produksi Alkaloid pada Kalus Berakar Datura metel L. terhadap Peningkatan Mikronutrien dari Medium MS Hadayani, Wahyu; Nurchayati, Yulita; Setiari, Nintya
JURNAL ANATOMI FISIOLOGI Vol 12, No 1 (2012): VOL XX, NOMOR 1, TAHUN 2012
Publisher : JURNAL ANATOMI FISIOLOGI

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Senyawa alkaloid dari tanaman Solanaceae memiliki potensi obat antara lain sebagai antibakteri bahkan memberi efek halusinasi. Alkaloid pada kecubung, Datura metel disintesis pada organ akar dan diakumulasi pada bagian pucuk. Salah satu metoda untuk produksi senyawa alkaloid tersebut adalah dengan menginduksi perakaran dari kalus yang berasal dari daun (induksi kalus berakar). Produksi alkaloid dapat ditingkatkan dengan memodifikasi komponen mikronutrien dari medium dasar MS (Murashige&Skoog).  Penelitan ini bertujuan untuk mempelajari pengaruh peningkatan konsentrasi mikronutrien terhadap pertumbuhan dan kandungan alkaloid total dari kalus berakar.  Kultur diperoleh dari induksi kalus dalam medium MS tanpa zat tumbuh dengan perlakuan konsentrasi mikronutrien.  Pertumbuhan kultur ditentukan dari berat segar dan berat kering,   sedangkan kandungan alkaloid total dianalisis dengan metoda titrasi. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan  bahwa peningkatan konsentrasi mikronutrien hingga 2,5 kali dari medium MS menghambat pertumbuhan kalus berakar. Namun demikian, kondisi ini tampak tidak mampu memacu produksi senyawa alkaloid dari kultur.
Pengaruh Pemberian Vitamin C (asam askorbat) terhadap Kesegaran Bunga Krisan (Chrysanthemum sp) pada Kawasan Sentra Penghasil di Desa Ngasem, Kecamatan Jetis, Bandungan, Jawa Tengah Arisanti, Desi; Setiari, Nintya
JURNAL ANATOMI FISIOLOGI Vol 12, No 1 (2012): VOL XX, NOMOR 1, TAHUN 2012
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Bunga Krisan (Chrysanthemum sp) merupakan salah satu jenis bunga potong yang popular dan banyak diminati oleh konsumen karena bentuk dan warnanya yang menarik serta ukuran yang bervariasi. Budidaya bunga krisan di Desa Ngasem merupakan mata pencaharian vital bagi petani karena sebagian besar bertanam bunga krisan. Permasalahan dalam budidaya bunga krisan potong yaitu penanganan pascapanen. Bunga yang dipotong, lama kesegarannya berkurang seiring menurunnya kandungan unsur hara dalam tanaman. Petani di Desa Ngasem mempertahankan kesegaran bunga potong krisan hanya direndam dalam air. Penelitian ini bertujuan untuk mengetahui cara penanganan pascapanen bunga potong krisan di Desa Ngasem dan mengetahui pengaruh pemberian vitamin C dalam larutan perendam (pulsing) sehingga dapat memperpanjang lama kesegaran bunga potong krisan. Perlakuan dalam percobaan adalah perbedaan komposisi larutan perendam bunga potong krisan, yaitu perlakuan P0 : larutan perendam berupa air 500 ml; perlakuan P1 : larutan perendam berupa campuran air 500 ml  dan  vitamin  C 100 ppm; perlakuan P2 : larutan perendam berupa campuan air 500 ml dan  vitamin  C 200 ppm. Masing-masing perlakuan diulang sebanyak 3 kali dengan rancangan percobaan berupa Rancangan Acak Lengkap (RAL) dengan pola faktor tunggal.. Parameter yang diamati yaitu lama kesegaran bunga potong krisan. Berdasarkan uji F hasil pengamatan menunjukkan bahwa lama kesegaran bunga potong krisan tidak dipengaruhi oleh pemberian vitamin C dalam larutan perendam tetapi hanya dipengaruhi oleh air tanpa pemberian vitamin C. Perlakuan air sebagai kontrol (perlakuan P0) mampu mempertahankan lama kesegaran bunga potong krisan tertinggi, yaitu 14 hari. Perlakuan dengan pemberian vitamin C (P1 dan P2) hanya mampu mempertahankan lama kesegaran bunga potong krisan selama 12 hari.
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Hibiscus sabdariffa L. dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Ascorbic acid and carotenoid are secondary metabolites found in roselle, which show antioxidant activity. These compounds can be obtained from callus induced  by several kinds of explants. The aims of this experiment is to study callus growth from explants which can encourage high level of antioxidant compounds. The callus was obtained from difference organ, i.e. section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L Naphtalene Acetic Acid (NAA) dan 5 mg/L Benzyl Adenin (BA).  This experiment has been conducted by completly randomized design with 5 replicates. Besides fresh weight callus, callus respons from each explants were analyzed descrptively.  Ascorbic acid and carotenoid content were analyzed qualitatively and quantitatively by titration and spectrophotometric respectively.  The results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds. Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Hibiscus sabdariffa L. dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
SAINS DAN MATEMATIKA Volume 22 Nomor 1 Tahun 2014
Publisher : SAINS DAN MATEMATIKA

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Abstract

Ascorbic acid and carotenoid are secondary metabolites found in roselle, which show antioxidant activity. These compounds can be obtained from callus induced  by several kinds of explants. The aims of this experiment is to study callus growth from explants which can encourage high level of antioxidant compounds. The callus was obtained from difference organ, i.e. section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L Naphtalene Acetic Acid (NAA) dan 5 mg/L Benzyl Adenin (BA).  This experiment has been conducted by completly randomized design with 5 replicates. Besides fresh weight callus, callus respons from each explants were analyzed descrptively.  Ascorbic acid and carotenoid content were analyzed qualitatively and quantitatively by titration and spectrophotometric respectively.  The results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds. Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid