M. Agus Setiadi
Departemen Klinik, Reproduksi, dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor

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The use enzyme immuno assay methode for measurement of milk progresterone without extraction for early pregnancy diagnosis in cow

Hemera Zoa Vol 77, No 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

An Enzyme Immuno Assay for Progesterone, using horse radish peroxidase as the label, was adapted for direct measurement of progesterone in milk. This was carriedout to detect early pregnancy in cows. The experiment used sixty milking cows divided into three groups, one as a controland two others as treatment groups. In group I (control) milk samples were collected on the day of insemination to 24 days afterward by quartan collection. In group II, milk samples were collected on days 18, 21 and 24 after insemination. In group III, milk samples were collected on days 21 and 24 after insemination. Milk samples were collected from lactating cows at noon from four quarters and preserved with Potassium dichromate (Merck 4858), stored at - 20 degree C until analysed.Pregnancy diagnosis by EIA was confirmed by rectal palpation at 60 to 90 days after insemination. The early pregnancy diagnosis in Group II compared with Group III showed that prolonged the time of sampling indeclinable improved the acuracy.

Motilitas dan Keutuhan Membran Plasma Spermatozoa Epididimis Kucing Selama Penyimpanan Pada Suhu 4°C

Media Kedokteran Hewan Vol 21, No 3 (2005): Media Kedokteran Hewan
Publisher : Media Kedokteran Hewan

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Abstract

The aim of this present study was to investigate the progressive motility and membrane integrity of cat epididymal spermatozoa during storage at 4°C.  Spermatozoa was collected by flushing technique with physiological saline and diluted in Tris egg yolk extender for three days.  The result showed that mean concentration of spermatozoa from cauda epididymal was 108,40 ± 81,49.106spz/ml.  The percentage of progressive motility and membrane integrity of spermatozoa on day-0 (time of collection) was 74,50 ± 3,50 % and  80,82 ± 2,00 %, respectively.  The percentage of progressive motility on day-1 was 62.5 ± 5.59 % and significantly higher(P<0,05)  than day-2 (50.5 ± 5.68 %) and  day-3 (25.5 ± 9.60 %).  There was also significantly different on the percentage of membrane integrity on day-1(71.54 ± 2.50 %), day-2 (64.16 ± 4.15 %) and day-3 (50.03 ± 4.16 %) of storage at 4°C.  In conclusion ,the spermatozoa derived from cat cauda epididymal could be used for artificial insemination after 2 days of storage at  4°C. Key words: epididymal sperm, storage, cat

The Effect of Vitamin E Supplement in the Diet on Gonad Maturation of Nilem (Osteochilus hasselti, CV)

Jurnal Perikanan Universitas Gadjah Mada Vol 19, No 1 (2017)
Publisher : Universitas Gadjah Mada

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Abstract

The aim of this study was to examine the effect of vitamin E on gonad maturation and determining the best doses in improving of gonad maturation in nilem fish. In this study used a flock of fish brood which is never experienced spawning. There are 4 doses of vitamin E which used in this research following by 0, 125, 250, and 375 mg kg-1. The results showed that fortified of vitamin E which were in feed significantly affected to the percentage of gonad development, gonado somatic index (IGS), hepato somatic index (IHS), fecundity, diameter of eggs, and the percentage of egg maturity at P<0.05. The result showed that the best dose of vitamin E to reach gonad maturity were 375 mg kg-1 with six weeks maintenanced. The percentage of gonad maturity was 100%, IGS was 11.50%, IHS was 0.34%, fecundity was 23.484 eggs/individual, the diameter of the eggs were 0.31 mm, and the percentage of mature eggs 64.44%. These results supported the potentially of feed additives such as vitamin E plays a critical role in enhancing gonad maturation in Nilem fish thereby providing a methods to improve fish reproduction.

KONSENTRASI, KEMURNIAN, DAN VIABILITAS SEL LEYDIG HASIL PURIFIKASI DENGAN GRADIEN NYCODENZ DAN KULTUR IN VITRO

Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan isolasi dan purifikasi sel Leydig dengan menggunakan gradien Nycodenz untuk meningkatkan jumlah perolehan sel Leydig yang hidup setelah purifikasi dan kultur. Isolasi dan purifikasi sel Leydig dilakukan dengan perlakuan gradien Nycodenz 3 kolom (5, 10, dan 15% sebagai Nycodenz I) dan 5 kolom (4, 8, 10, 12, dan 15% sebagai Nycodenz II) serta gradien Percoll 5 kolom (21, 26, 34, 40 dan 60%) sebagai kontrol. Parameter yang diamati adalah konsentrasi, kemurnian, dan viabilitas sel Leydig setelah isolasi dan purifikasi serta kultur in vitro. Hasil yang diperoleh menunjukkan bahwa isolasi dan purifikasi sel Leydig dengan gradien Nycodenz (I dan II) secara nyata (P<0,01) menghasilkan konsentrasi sel yang lebih rendah dibandingkan dengan gradien Percoll. Namun penggunaan gradien Nycodenz II c enderung menghasilkan kemurnian sel yang lebih tinggi (91,40%) dibandingkan dengan Nycodenz I (85,53%). Hasil tersebut tidak berbeda nyata dibandingkan dengan gradien Percoll (92,20%). Viabilitas sel Leydig pada semua perlakuan hampir sama yaitu 98%. Namun demikia n, setelah dikultur selama 3 hari, konsentrasi sel Leydig pada perlakuan Percoll (2,44x106 sel/ml) dan Nycodenz I (3,21x106 sel/ml) secara statistik (P<0,05) lebih rendah dan dibandingkan dengan Nycodenz II (3,88x10 6 sel/ml), sedangkan viabilitas sel Leydig setelah dikultur pada gradien Nycodenz I (90,00%) dan Nycodenz II (91,17%) secara sangat nyata (P<0,01) menghasilkan viabilitas yang lebih tinggi dibandingkan dengan gradien Percoll (82,30%). Berdasarkan hasil tersebut dapat disimpulkan bahwa penggunaan gradien Nycodenz II efektif untuk mempurifikasi sel Leydig dan setelah dikultur menghasilkan konsentrasi dan viabilitas sel yang lebih tinggi dibandingkan dengan gradien Percoll.

Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK)

Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

In poultry embryos, primordial germ cells (PGCs) are progenitor cells for gametes, which have unique migration pathway. Primordial germ cells arise from epiblast in germinal crescent and circulate through the bloodstream for a short period of time, then leave blood vessel to migrate toward gonads. The aim of this study was to determine the potential production capacity of circulated-PGCs of KUB chicken at different developmental stages of embryo using a rapid and simple method. Seventy five KUB chicken fertile eggs were divided into five groups and incubated at 38.5 0C with a humidity of 60%. Hatching was set to the embryonic development stage of 14-18. The blood was collected through dorsal aorta using micropipette under microscope. The collected blood was placed in a 1.5 ml eppendorf tube which was previously filled with 100 µl phosphate buffered saline without Ca2+ and Mg2+ (PBS-) mixed with fetal bovine serum (FBS) with a ratio of 90%:10%. The PGCs were purified using lysis buffer ammonium chloride potassium method. The results showed that average production of circulated-PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P <0.05). The average production of circulated-PGCs at stage 14, 15, 16, 17, and 18 were 37.9; 53.5; 49.8; 38.3; and 33.5 respectively. The number of circulated-PGCs was not different among stages 14, 17 nor 18. The highest number of circulated-PGCs of KUB chicken was obtained at stage 15, so that the isolation and collection of PGCs through the blood circulation was recommended in stage 15.Key Words: KUB Chicken, PGCs, Embryonic Development Stage, Ammonium Chloride Potassium