Articles

Found 30 Documents
Search

Molecular Phylogenetic Classification of Streptomycetes Isolated from the Rhizosphere of Tropical Legume (Paraserianthes falcataria) (L.) Nielsen

HAYATI Journal of Biosciences Vol 16, No 3 (2009): September 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (475.107 KB)

Abstract

Intrageneric diversity of 556 streptomycetes isolated from the rhizosphere of tropical legume was determined by using molecular taxonomic method based on 16S rDNA. A total of 46 isolates were taken to represent 37 colour groups of the isolates. 16S rDNA were amplified and subsequently sequenced and the sequences data were aligned with streptomycete sequences retrieved from the ribosomal data base project (RDP) data. Phylogenetic trees were generated by using the PHYLIP software package and the matrix of nucleotide similarity and nucleotide difference were generated by using PHYDIT software. The results confirmed and extended the value of 16S rDNA sequencing in streptomycete systematic. The 16S rDNA sequence data showed that most of the tested colour group representatives formed new centers of taxonomic variation within the genus Streptomyces. The generic assignment of these organisms was underpinned by 16S rDNA sequence data which also suggested that most of the strains represented new centers of taxonomic variation. The taxonomic data indicate that diverse populations of streptomycetes are associated with the roots of tropical legume (P. falcataria). Therefore, the combination of selective isolation and molecular taxonomic procedures used in this study provide a powerful way of uncovering new centers of taxonomic variation within the genus Streptomyces. Key words: molecular phylogenetic, classification, streptomycetes, rhizosphere, Paraserianthes falcatharia

Application of Numerical Systematics in Unraveling Streptomycete Diversity

Jurnal Mikrobiologi Indonesia Vol 6, No 1 (2001): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (746.922 KB)

Abstract

Systematic. play. an important role in the utilization of streptomycete strains as an outstanding producer ofthousands documented bloactive compounds. The chaotic state of streptomycete systematics resulted from the application of traditional monothetic approach had clearly hampered the progress of development of reliable Identification system for potential streptomycete strains. However, the extcnSivC application of polythitic numerical method byincreasing number of experts was proved to be successful in developing a more sounding streptomycete elasslflcstl*nsystem. As a consequence, such classification System can subsequently be used as a rigorous basis to generate a morereliable identification system in attempt to unravel the extent of streptomycete diversity In natural habitats both itthe Inter and intraspecies level. This review addresses the problem and the role of numerical systematic. in thedevelopment of current status of streptomycete systematics which is applicable to delimit species within the genus.

OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (Optimization Of Fermentation Conditions For The Production Of Bacterial Cellulose By Slk-1 Strain In Coconut Water Based Medium)

Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (426.082 KB)

Abstract

ABSTRAK   Tujuan penelitian ini adalah mengoptimasi kondisi fermentasi terbaik strain Bakteri Asam Asetat penghasil selulosa yaitu isolat SLK-1.  Strain ini diisolasi dari buah salak pada penelitian sebelumnya.  Hasil optimasi menunjukkan bahwa kondisi fermentasi optimum untuk pertumbuhan dan produksi selulosa pada isolat SLK-1  dicapai dengan sumber karbon gula pasir, sumber nitrogen ammonium sulfat, pH 7, suhu inkubasi 25°C dan metode fermentasi statis. Karakter struktur permukaan selulosa hasil fermentasi isolat SLK-1 dipengaruhi oleh metode fermentasi yang digunakan.  Metode fermentasi goyangan berpengaruh menurunkan produksi selulosa pada  isolat SLK-1 dan merubah struktur permukaan yaitu susunan mikrofibril lebih renggang dan membentuk gelembung.   Kata Kunci: bakteri asam asetat, optimasi, fermentasi, selulosa bakteri, penggoyangan

KEANEKARAGAMAN SPESIES BAKTERI PADA KULTUR DARAH WIDAL POSITIF ASAL KOTA SEMARANG BERDASARKAN KARAKTER FENOTIPIK

Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (356.083 KB)

Abstract

ABSTRAK   Tujuan penelitian ini untuk menentukan keanekaragaman spesies bakteri pada kultur darah Widal positif  Asal kota Semarang berdasrkan karakter fenotipik. Sampel darah yang dikultur sebanyak 136 sampel berasal dari pasien rawat inap dan rawat jalan di 4 rumah sakit serta 2 puskesmas di kota Semarang (RSUD Kota Semarang, RSUD Tugurejo, RS. Islam Sultan Agung,  dan 2 Puskesmas yaitu Kedungmundu dan Bangetayu.  Kultur darah digunakan medium BacT/Alert FAN blood culture bottles (Biomerieux), subkultur digunakan medium Blood Agar Plate (BAP, OXOID) dan Mac Conkey (MC, OXOID), dilanjutkan  uji biokimia digunakan medium API 20E dan API 50CHB/E untuk identifikasi strain anggota familia Enterobacteriaceae serta APIStap (Biomerieux) untuk identifikasi spesies anggota Staphylococcus. Kultur darah positif sebanyak 59 sampel (43.4%) terdiri dari 44 sampel (32,4%) positif Staphylococcus sp. (S. aureus, S. saprophyticus, S. xylosus, S. warnei, S. hominis, S. cohnii) dan 15 sampel (11%) positif bakteri batang gram negatif anggota familia Enterobacteriaceae yaitu Enterobacter cloacae, S. typhi, Serratia marcescens, Escherichia coli, Salmonella ssp., Klebsiella pneumoniae ssp. Ozanae. Berdasarkan karakter fenotipik  bakteri batang gram negatif dapat dikelompokkan menjadi 4 kluster, kluster pertama beranggotakan S. typhi , kluster kedua beranggotakan E. coli dan Salmonella ssp., kluster ketiga beranggotakan Ser. Marcescens dan kluster keempat beranggotakan Enterobacter cloacae dan Kleb. pneumoniae ssp. Ozaenae. Bakteri kokus gram positif berdasarkan karakter fenotipiknya dapat dikelompokkan menjadi 6 kluster  yang tampak sangat bervariasi   Kata kunci:  Widal, Kultur darah, BacT/Alert FAN, API 20E, API 50 CHB/E, API Stap

Ecological Approach to Unravel Streptomycete Diversity as an Unsurpassed Sources of Natural Bioactive Products

Microbiology Indonesia Vol 2, No 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Original Source | Check in Google Scholar

Abstract

Search and discovery for natural bioactive products have been so important to control the emergence of antibiotic resistant microbial pathogens. Therefore, novel microorganisms that produce such metabolites is extremely needed. The capacity of members of the genus Streptomyces to produce commercially significant bioactive metabolites, notably antibiotics remains unsurpassed. However, it is acknowledged that discovering commercially useful secondary metabolites from streptomycetes is becoming more difficult due to lack of knowledge on the ecology and complexity of streptomycete systematics. In fact, those are fundamental aspects for developing strategy and method for isolation. In order to devise an appropriate program for successful selective isolation of sreptomycetes, it is fundamentally important to understand their occurance and activity in nature. A multistep extraction procedure designed for representative sampling, called dispersion, and differential centrifugation technique in combination with the incorporation of antibiotics into isolation media has become one of the most important selective method for the isolation of streptomycetes from natural habitats. The availability of new procedures to selectively isolate representative of streptomycetes from natural habitats opens up the possibility to determine the extent of streptomycete diversity from various habitats. Hence, the capacity of well characterized streptomycete isolates to produce commercial novel active metabolites could be further assessed appropriately.

OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (Optimization Of Fermentation Conditions For The Production Of Bacterial Cellulose By Slk-1 Strain In Coconut Water Based Medium)

Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (426.082 KB)

Abstract

ABSTRAK   Tujuan penelitian ini adalah mengoptimasi kondisi fermentasi terbaik strain Bakteri Asam Asetat penghasil selulosa yaitu isolat SLK-1.  Strain ini diisolasi dari buah salak pada penelitian sebelumnya.  Hasil optimasi menunjukkan bahwa kondisi fermentasi optimum untuk pertumbuhan dan produksi selulosa pada isolat SLK-1  dicapai dengan sumber karbon gula pasir, sumber nitrogen ammonium sulfat, pH 7, suhu inkubasi 25°C dan metode fermentasi statis. Karakter struktur permukaan selulosa hasil fermentasi isolat SLK-1 dipengaruhi oleh metode fermentasi yang digunakan.  Metode fermentasi goyangan berpengaruh menurunkan produksi selulosa pada  isolat SLK-1 dan merubah struktur permukaan yaitu susunan mikrofibril lebih renggang dan membentuk gelembung.   Kata Kunci: bakteri asam asetat, optimasi, fermentasi, selulosa bakteri, penggoyangan

KEANEKARAGAMAN SPESIES BAKTERI PADA KULTUR DARAH WIDAL POSITIF ASAL KOTA SEMARANG BERDASARKAN KARAKTER FENOTIPIK

Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (356.083 KB)

Abstract

ABSTRAK   Tujuan penelitian ini untuk menentukan keanekaragaman spesies bakteri pada kultur darah Widal positif  Asal kota Semarang berdasrkan karakter fenotipik. Sampel darah yang dikultur sebanyak 136 sampel berasal dari pasien rawat inap dan rawat jalan di 4 rumah sakit serta 2 puskesmas di kota Semarang (RSUD Kota Semarang, RSUD Tugurejo, RS. Islam Sultan Agung,  dan 2 Puskesmas yaitu Kedungmundu dan Bangetayu.  Kultur darah digunakan medium BacT/Alert FAN blood culture bottles (Biomerieux), subkultur digunakan medium Blood Agar Plate (BAP, OXOID) dan Mac Conkey (MC, OXOID), dilanjutkan  uji biokimia digunakan medium API 20E dan API 50CHB/E untuk identifikasi strain anggota familia Enterobacteriaceae serta APIStap (Biomerieux) untuk identifikasi spesies anggota Staphylococcus. Kultur darah positif sebanyak 59 sampel (43.4%) terdiri dari 44 sampel (32,4%) positif Staphylococcus sp. (S. aureus, S. saprophyticus, S. xylosus, S. warnei, S. hominis, S. cohnii) dan 15 sampel (11%) positif bakteri batang gram negatif anggota familia Enterobacteriaceae yaitu Enterobacter cloacae, S. typhi, Serratia marcescens, Escherichia coli, Salmonella ssp., Klebsiella pneumoniae ssp. Ozanae. Berdasarkan karakter fenotipik  bakteri batang gram negatif dapat dikelompokkan menjadi 4 kluster, kluster pertama beranggotakan S. typhi , kluster kedua beranggotakan E. coli dan Salmonella ssp., kluster ketiga beranggotakan Ser. Marcescens dan kluster keempat beranggotakan Enterobacter cloacae dan Kleb. pneumoniae ssp. Ozaenae. Bakteri kokus gram positif berdasarkan karakter fenotipiknya dapat dikelompokkan menjadi 6 kluster  yang tampak sangat bervariasi   Kata kunci:  Widal, Kultur darah, BacT/Alert FAN, API 20E, API 50 CHB/E, API Stap

UJI AKTIVITAS ANTIBAKTERI ISOLAT ACTINOMYCETES DARI RIZOSFER PADI (Oryza sativa) TERHADAP Salmonella typhosa DAN Staphylococcus aureus

Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (393.06 KB)

Abstract

Actinomycetes merupakan anggota bakteri yang dipromosikan sebagai penghasil zat  antimikrobial  terbesar.  Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi Actinomycetes dari rizosfer padi (Oryza sativa) yang berpotensi sebagai penghasil antibakteri dan dapat menghambat pertumbuhan Salmonella typhosa dan Staphylococcus aureus. Penelitian ini menghasilkan 17 isolat Actinomycetes, 8 isolat diantaranya mampu menghasilkan zat antibakteri. Diantara 8 isolat tersebut, satu isolat mampu menghambat pertumbuhan kedua bakteri uji, yaitu NRPR 13 yang menghambat Salmonella typhosa (dengan diameter daerah hambatan 14 mm) dan Staphylococcus aureus (12 mm). Tiga isolat mampu menghambat pertumbuhan bakteri Salmonella  typhosa, yaitu NRPR  14  (11 mm), NRPR  62 dan NRPR 67 (masing-masing 10 mm). Dan empat isolat mampu menghambat pertumbuhan Staphylococcus aureus, yaitu RPR 15 (14 mm),  NRPR 11 (17 mm), NRPR 33 (11 mm) dan NRPR 46 (16 mm).  Berdasarkan hasil penelitian ini dapat disimpulkan bahwa Actinomycetes yang diisolasi dari rizosfer padi berpotensi menghasilkan zat antibakteri. Kata kunci : Actinomycetes,  Zat Antibakteri, S. typhosa,  S. aureus.

APLIKASI METODE ARDRA DALAM IDENTIFIKASI ISOLAT Bacillus thuringiensis ENDOGENIK SEBAGAI PENGENDALI HAMA KUBIS (Crocidolomia binotalis)

EUGENIA Vol 17, No 2 (2011)
Publisher : EUGENIA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (102.255 KB)

Abstract

ABSTRACT Indonesian indigenous bacterial isolates of B. thuringiensis pathogenic to cabbage pest (C. binotalis) were molecularly characterized and identified using DNA fingerprinting method of ARDRA (Amplified Ribosomal DNA Restriction Analysis). Chromosomal DNA of 10 selected isolates (SLK2.3, SRNG4.2, TKO1, TK9, YPPA1, UG1A, BLPPN8.2, YWKA1, BAU3.2, LPST1) and 2 reference strains (B. thuringiensis serovar kurstaki HD1 & B. thuringiensis serovar israelensis H14) were isolated and purified by standard method. 16S rRNA genes were amplified by PCR method using universal primers of 27f and 1529r. PCR products were digested by 4 restriction endonucleases (EcoR1, HindIII, Pst1 dan HaeIII), and separated by agarose electrophoresis method to generate ARDRA profiles. Results of study showed that only ARDRA profiles generated by Hae III digestion were found to be meaningful and therefore used to identify the isolates. The ARDRA profile analysis indicated that the reference strain of B. thuringiensis serovar kurstaki HD1 could be clearly separated with B. thuringiensis serovar israelensis H14. In fact, those two strains have been widely recognized to be different in terms of their pathogenic specifity against insects. B. thuringiensis serovar kurstaki HD1 has been known to be specifically pathogenic to Lepidopteran whereas B. thuringiensis serovar israelensis H14 has been known to be specifically pathogenic to Dipteran. Key words : application, ARDRA, indigenous, B. thuringiensis, C. binotalis  

A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria

Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar

Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB.Keywords : Production, PHB, Amylolytic bacteria, Sago starch.