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Diallel Analysis of Chili (Capsicum annuum L.) Resistance to Phytophthora capsici Leonian Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihat, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 3 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

 To accomplish the study of genetic parameters of chili resistance to P. capsici, thirty cross combinations from six parents’ crosses were made by full diallel method.  The resistance was assessed based on the incidence of disease, following the inoculation of 28 days-old plant of chili.  Inoculation was done by dropping 5 mL of inoculum (105 zoospore mL-1) at the base part of each plant. There was non-allelic interaction and overdominance effect. Chili resistance to P. capsici controlled by   one   positive   gene.  The  parents   contained   more  dominant  gene, with dominance order were IPB-C4, IPB-C10, IPB-C15,  IPB-C9,  IPB-C8,  and  IPB-C2. The prediction of the possible limits of selection if homozygote genes assemble on one individual was 0.676-0.691. Broad-sense and narrow-sense heritability values for the traits were high.    Keywords: chili, diallel analysis, genetic parameter, Phytophthora capsici  
Kriteria Seleksi untuk Perakitan Varietas Cabai Tahan Phytophthora capsici Leonian Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Selection criteria for developing Phytophthora blight-resistant varieties of pepper had been done in both laboratory and fi  eld conditions. Resistance screening were conducted on 28-days-old pepper plants grown in 72-cell fl  ats by inoculating 5 mL of inoculum (contain 105 zoospore mL-1) to the base of each plant. Phytophthora capsici isolate used in this experiment was TG01, which then identifi  ed as race-3. The evaluation of pepper genotypes characteristics were conducted in fi  eld.  Eight characters demonstrated a wide genetic variability; those characters were resistance to Phytophthora blight, infection period, dicotomous height, canopy width, fruit weight, fruit length, fruit diameter and production. These characters along with fruit wall thickness, chlorophyll-a, chlorophyll-b, total chlorophyll had high heritability values. Using path analysis infection period, dicotomous height and fruit diameter are recommended to be simultaneously used as selection criteria for developing Phytophthora blight resistant pepper varieties. Keywords : capsicum, Phytophthora capsici, selection criteria, path analysis
Induksi Mutasi pada Stek Pucuk Anyelir (Dianthus caryophyllus Linn.) melalui Iradiasi Sinar Gamma Aisyah, Syarifah Iis; Aswidinnoor, Hajrial; Saefuddin, Asep; Marwoto, Budi; Sastrosumarjo, Sarsidi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 1 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

It has been a common knowledge that LD50 is commonly used in estimating optimal doses of gamma irradiation in a breeding program. This research was aimed at observing radiosensitivity of five carnation´s genotypes to gamma irradiation, to find the LD50 of carnation´s cuttings, and to obtain solid mutants from five numbers of Carnation.  For cuttings, carnation genotype number 10.8 was the most insensitive to gamma rays, whereas number 24.15 was the most sensitive one.  LD50 of carnation´s cuttings were obtained around 49 -72 gray. There were 19 mutants produced from this treatment. The desired mutans were mostly produced from the treated 24.1 genotype whereas the character mutans were mostly observed in MV2 generation. Irradiation treatment on genotype 24.1 produced most stabile mutans while the less was in genotype 24.14.  The produced mutants were qualitatively different in colour and petal shape, and stabile till third generation.   Key words: LD50, gamma irradiation, induced mutation, carnation.
Ketahanan 22 Genotipe Cabai (Capsicum spp.) terhadap Phytophthora capsici Leonian dan Keragaman Genetiknya Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Laboratory and field experiments were carried out to analyze genetic diversity of 22 pepper genotypes (Capsicum spp.) and their resistance to Phytophthora capsici Leonian. Resistance screening was performed in plastic flats 72 cells. Inoculation was done on 28-day old pepper plant soon after watering by pipeting 5 ml of inoculum (105 zoospore/ml) at the base of each plant. P. capsici  isolate used in this experiment was TG01, identified as race 3 based on AVRDC differential pepper lines (PI 188478, PBC 602, PBC 137 and Early Calwonder).  The evaluation of pepper genotypes characteristic was conducted in the field.  Principle Component Analysis, Clustering Analysis and Biplot Analysis were used to analyze genetic diversity based on 37 characters. Result of resistance evaluation showed that two genotypes (C4 and C13) were identified as resistant, 7 genotypes (C2, C3, C5, C8, C10, C15, and C20) as slightly resistant, 6 genotypes (C7, C9, C17, C19, C21 and C27) as slightly susceptible, and 7 genotypes (C1, C11, C18, C28, C48, C64, and C65) as susceptible.  Based on genetic diversity analyzed, all genotypes could be divided into 4 clusters.  Cluster I consisted of 18 genotypes i.e. C1, C2, C3, C4, C5, C7, C8, C9, C10, C13, C14, C15, C17, C18, C19, C28, C64, and C65. Cluster II consisted of only 1 genotype i.e. C48.  Cluster III consisted of 2 genotypes (C20 and C21) which were characterized by the colour of corolla, corolla spot, and filament. Cluster IV consisted of 1 genotype (C27) which was characterized by fruit cross-sectional corrugation.   Key words :  Capsicum, resistance, Phytophthora capsici, genetic diversity, clustering.
ANALISIS MARKA MORFOLOGI DAN MOLEKULER SIFAT KETAHANAN KEDELAI TERHADAP INTENSITAS CAHAYA RENDAH Handayani, Titin; Sastrosumarjo, Sarsidi; Sopandie, Didy; Suharsono, Suharsono; Setiawan, Asep
Jurnal Sciene dan Teknologi Vol 8, No 1 (2006): JURNAL SAINS DAN TEKNOLOGI INDONESIA
Publisher : Jurnal Sciene dan Teknologi

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Abstract

Availability of moleculer marker to identify the important agronomic character of plant is needed to accelerate selection activity of plant. Particularly for the complex quantitatively inherited traits - like e.g. shading tolerance of soybean - the use of such a technique will speed up the process to produce adapted genotypes.The objective of this research is to identify the linkage of molecular marker RAPD with character of shading tolerance. The morphological specific characters wich is corelated to shade tolerance is the number of productive branchs. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes. The inheritance of shading tolerance of soybean was controlled by gene with full dominant or by two genes pairs with dominant and ressesive epistasis. There was no maternal effect in the inheritance to shade tolerance. Heritability value (0.45 – 0.54) indicated that the proportion variation caused by the genetic factors was moderate. Molecular analysis by using RAPD technique showed that UBC153, ROTH 480.01, and ROTH 480.03 primer have polymorphic band that can be used for inheritance study and linkage analysis. All polymorphisms segregated independently of each ather. Interval mapping with Mapmaker/QTL indicated that the location of the three QTLs on linkage group were at marker tolerance locus of Roth 480.01-8125 , Roth 480.03-1125, and UBC 153-19125 .
ANALISIS MARKA MORFOLOGI DAN MOLEKULER SIFAT KETAHANAN KEDELAI TERHADAP INTENSITAS CAHAYA RENDAH Handayani, Titin; Sastrosumarjo, Sarsidi; Sopandie, Didy; Suharsono, Suharsono; Setiawan, Asep
Jurnal Sains dan Teknologi Indonesia Vol 8, No 1 (2006)
Publisher : Jurnal Sains dan Teknologi Indonesia

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Abstract

Availability of moleculer marker to identify the important agronomic character of plant is needed to accelerate selection activity of plant. Particularly for the complex quantitatively inherited traits - like e.g. shading tolerance of soybean - the use of such a technique will speed up the process to produce adapted genotypes.The objective of this research is to identify the linkage of molecular marker RAPD with character of shading tolerance. The morphological specific characters wich is corelated to shade tolerance is the number of productive branchs. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes. The inheritance of shading tolerance of soybean was controlled by gene with full dominant or by two genes pairs with dominant and ressesive epistasis. There was no maternal effect in the inheritance to shade tolerance. Heritability value (0.45 – 0.54) indicated that the proportion variation caused by the genetic factors was moderate. Molecular analysis by using RAPD technique showed that UBC153, ROTH 480.01, and ROTH 480.03 primer have polymorphic band that can be used for inheritance study and linkage analysis. All polymorphisms segregated independently of each ather. Interval mapping with Mapmaker/QTL indicated that the location of the three QTLs on linkage group were at marker tolerance locus of Roth 480.01-8125 , Roth 480.03-1125, and UBC 153-19125 .
Ketahanan 22 Genotipe Cabai (Capsicum spp.) terhadap Phytophthora capsici Leonian dan Keragaman Genetiknya Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Laboratory and field experiments were carried out to analyze genetic diversity of 22 pepper genotypes (Capsicum spp.) and their resistance to Phytophthora capsici Leonian. Resistance screening was performed in plastic flats 72 cells. Inoculation was done on 28-day old pepper plant soon after watering by pipeting 5 ml of inoculum (105 zoospore/ml) at the base of each plant. P. capsici? isolate used in this experiment was TG01, identified as race 3 based on AVRDC differential pepper lines (PI 188478, PBC 602, PBC 137 and Early Calwonder).? The evaluation of pepper genotypes characteristic was conducted in the field. ?Principle Component Analysis, Clustering Analysis and Biplot Analysis were used to analyze genetic diversity based on 37 characters. Result of resistance evaluation showed that two genotypes (C4 and C13) were identified as resistant, 7 genotypes (C2, C3, C5, C8, C10, C15, and C20) as slightly resistant, 6 genotypes (C7, C9, C17, C19, C21 and C27) as slightly susceptible, and 7 genotypes (C1, C11, C18, C28, C48, C64, and C65) as susceptible.? Based on genetic diversity analyzed, all genotypes could be divided into 4 clusters.? Cluster I consisted of 18 genotypes i.e. C1, C2, C3, C4, C5, C7, C8, C9, C10, C13, C14, C15, C17, C18, C19, C28, C64, and C65. Cluster II consisted of only 1 genotype i.e. C48.? Cluster III consisted of 2 genotypes (C20 and C21) which were characterized by the colour of corolla, corolla spot, and filament. Cluster IV consisted of 1 genotype (C27) which was characterized by fruit cross-sectional corrugation. ? Key words : ?Capsicum, resistance, Phytophthora capsici, genetic diversity, clustering.
Kriteria Seleksi untuk Perakitan Varietas Cabai Tahan Phytophthora capsici Leonian Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Selection criteria for developing Phytophthora blight-resistant varieties of pepper had been done in both laboratory and ?? eld conditions. Resistance screening were conducted on 28-days-old pepper plants grown in 72-cell ?? ats by inoculating 5 mL of inoculum (contain 105 zoospore mL-1) to the base of each plant. Phytophthora capsici isolate used in this experiment was TG01, which then identi?? ed as race-3. The evaluation of pepper genotypes characteristics were conducted in ?? eld.? Eight characters demonstrated a wide genetic variability; those characters were resistance to Phytophthora blight, infection period, dicotomous height, canopy width, fruit weight, fruit length, fruit diameter and production. These characters along with fruit wall thickness, chlorophyll-a, chlorophyll-b, total chlorophyll had high heritability values. Using path analysis infection period, dicotomous height and fruit diameter are recommended to be simultaneously used as selection criteria for developing Phytophthora blight resistant pepper varieties. Keywords : capsicum, Phytophthora capsici, selection criteria, path analysis
Induksi Mutasi pada Stek Pucuk Anyelir (Dianthus caryophyllus Linn.) melalui Iradiasi Sinar Gamma Aisyah, Syarifah Iis; Aswidinnoor, Hajrial; Saefuddin, Asep; Marwoto, Budi; Sastrosumarjo, Sarsidi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

It has been a common knowledge that LD50 is commonly used in estimating optimal doses of gamma irradiation in a breeding program. This research was aimed at observing radiosensitivity of five carnation's genotypes to gamma irradiation, to find the LD50 of carnation's cuttings, and to obtain solid mutants from five numbers of Carnation.? For cuttings, carnation genotype number 10.8 was the most insensitive to gamma rays, whereas number 24.15 was the most sensitive one.? LD50 of carnation's cuttings were obtained around 49 -72 gray. There were 19 mutants produced from this treatment. The desired mutans were mostly produced from the treated 24.1 genotype whereas the character mutans were mostly observed in MV2 generation. Irradiation treatment on genotype 24.1 produced most stabile mutans while the less was in genotype 24.14.? The produced mutants were qualitatively different in colour and petal shape, and stabile till third generation. ? Key words: LD50, gamma irradiation, induced mutation, carnation.