Ediati Sasmito
Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia

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Isolasi Dan Identifikasi Senyawa Flavonoid Subfraksi Etil Asetat Myrmecodia tuberosa (non Jack) Bl Khumaidi, Akhmad; Hertiani, Triana; Sasmito, Ediati
Natural Science: Journal of Science and Technology Vol 4, No 2 (2015): Volume 4 Number 2 (August 2015)
Publisher : Univ. Tadulako

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Berdasarkan studi sebelumnya diperoleh bahwa kadar flavonoid total subfraksi etil asetat Myrmecodia tuberosa (non Jack) Bl memiliki korelasi positif terhadap aktivitas proliferasi sel limfosit. Subfraksi V (kelima) menunjukkan nilai indeks stimulasi proliferasi sel limfosit tertinggi serta memiliki kadar flavonoid total terbesar (6,158±1,298) diantara semua subfraksi. Penelitian ini bertujuan mengisolasi dan mengidentifikasi senyawa flavonoid yang dikandung oleh subfraksi V. Metode pemisahan yang dilakukan adalah melakukan hidrolisis subfraksi V, kemudian dikromatografi lapis tipis preparatif dan dianalisis secara spektroskopi. Hasil spektroskopi  UV-Vis isolat memiliki puncak 1 pada panjang gelombang 324 nm dan puncak 2 pada panjang gelombang 284 nm, spektroskopi infra merah memiliki serapan pada 3.443 cm-1. Data 1H-NMR menunjukkan adanya serapan pada ? 6,74; ? 6,73; ? 6,62; ? 6,10; ? 7,82; ? 7,39; doublet of doublet pada ? 1,14, doublet pada ? 3,47 - ? 3,60 dan singlet pada ? 3,65 serta adanya serapan pada ? 17,81; 71,47; 74,19; 74,51 dan ? 76,78 dari spektrum 13C-NMR. Hasil spektroskopi massa terlihat serapan pada m/z 391,10. Berdasarkan data-data yang diperoleh, senyawa hasil isolasi diduga merupakan senyawa isoflavon.
UJI AKTIVITAS IMUNOSTIMULATOR EKSTRAK ETANOL DAN FRAKSI–FRAKSI KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) TERHADAP PROLIFERASI SEL LIMFOSIT MENCIT GALUR SWISS SECARA IN VITRO BESERTA IDENTIFIKASI KANDUNGAN KIMIANYA Ulfah, Maria; Nirmalasari, Nirmalasari; Yulianti, Desi; Sakti, Riyanto; Heni, Oktarina; Sasmito, Ediati
Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Vol 1, No 1 (2014): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 5 2014
Publisher : Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik

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Agen imunostimulator berperan penting dalam pengobatan penyakit infeksi akibat patogen. Kelopak bunga rosella (Hibiscus sabdariffa L.) dimanfaatkan oleh masyarakat sebagai minuman kesehatan untuk meningkatkan sistem imun tubuh. Penelitian ini bertujuan untuk mengetahui aktivitas imunostimulator fraksi etil asetat ekstrak etanol kelopak bunga rosella terhadap proliferasi sel limfosit mencit galur swiss berserta identifikasi kandungan kimianya. Ekstraksi kelopak bunga rosella dilakukan dengan metode maserasi menggunakan pelarut etanol 96%, kemudian dilanjutkan fraksinasi secara bertingkat dengan menggunakan n-heksana, etil asetat dan air. Sampel uji ekstrak etanol, fraksi n-heksana, etil asetat dan air masing-masing dibuat dengan konsentrasi 10, 20, 50, 100, 200, 400  µg/mL dan kontrol positif PHA 10 µg/mL untuk diujikan terhadap kultur sel limfosit. Uji aktivitas imunostimulator menggunakan metode MTT Assay. Aktivitas proliferasi sel limfosit dianalisis secara statistik terhadap nilai Optical Density (OD) dari Enzyme Linked Immunosorbent Assay (ELISA) reader menggunakan statistik parametrik uji Oneway Anova dilanjutkan uji Tukey (p<0,05), serta statistik non parametrik Kruskal Wallis Test dilanjutkan Mann Whitney Test (p<0,05) yang dibandingkan terhadap masing-masing kontrol sel limfosit. Identifikasi kandungan senyawa fenolik dan flavonoid dilakukan dengan Kromatografi Lapis Tipis (KLT). Hasil penelitian menunjukkan bahwa kelopak bunga rosella memiliki aktivitas imunostimulator terhadap proliferasi sel limfosit yang terlihat pada konsentrasi 50, 100, 200 dan 400 µg/mL untuk ekstrak etanol dan fraksi etil asetat; 200 dan 400 µg/mL untuk fraksi n-heksana serta 400 µg/mL untuk fraksi air. Ekstrak etanol dan fraksi dari kelopak bunga rosella memiliki kandungan senyawa terpenoid, fenolik dan flavonoid. Kata kunci : kelopak bunga rosella, ekstrak etanol, fraksi,  MTT Assay, imunostimulator
Elettaria cardamomum Distillate Increases Cellular Immunity in Doxorubicin Treated Rats Raksamiharja, Rikat; Sy, Khairunnisa; S, Meirizky Zulharini; Novarina, Annisa; Sasmito, Ediati
Indonesian Journal of Cancer Chemoprevention Vol 3, No 3 (2012)
Publisher : Indonesian Research Gateway

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Doxorubicin is one of chemotherapeutic agent used for cancer therapy. However, doxorubicin usage causes some adverse effects, such as lymphocyte, CD4+ and CD8+ cells number.  Therefore,  the  co-chemotherapeutic  agent  is  required  to  reduce  the imunosuppression  effect.  Cardamom  (Elettaria  cardamomum)  contains  terpenoid  1,8  cineol. This  research  aimed  to  know  the  effect  of  Elettaria  cardamomum  distillate  (ECD)  in combination with doxorubicin on Sprague Dawley rat’s hematology profile and the amount of CD4+  and  CD8+  cells.  The  experiment  was  done  for  13  days  using  6  groups  of  rats:  I doxorubicin (dox) 15 mg/kg BW; II dox + ECD5 mg/kg BW; III dox + ECD 50 mg/kg BW; IV dox  +  ECD  100  mg/kg  BW;  V  ECD  100  mg/kg  BW;  VI  control  without  treatment.  The hematology  profile  and  the  amount  of  CD4+  and  CD8+  were  counted  before  and  after treatment  using  flowcytometer.  The  results  show  that  ECD  increases  the  amount  of lymphocyte, white blood, CD4+ and CD8+ cells in dose dependent manner in doxorubicin treated rats. Based on the data, it can be concluded that ECD is potential to be developed as immunostimulant agent for chemotherapy.Keywords: Cardamom (Elettaria cardamomum), immunostimulant, hematology, CD4+, CD8+
Optimum Dose and Formulation of Centella asiatica L. Urban Extract Against IgG of Wistar Strain Male Mices which Induced by BCG Vaccine Ermawati, Dian Eka; Sasmito, Ediati; Mufrod, Mufrod; ND., Pramitha Esha; UA., Ni Putu; KD, Anggi; H., Muchammad; S., Aini
Journal of Food and Pharmaceutical Sciences Vol 4, No 3 (2016): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/jfps

Abstract

Objective: Centella asiatica L.Urban contains triterphenoid saphonin, flavonoid, and pectin that believed to have an immunomodulatory effect toward the Immunoglobulin-G in Wistar strain male mices which Induced by BCG vaccine. A study was conducted to find out the optimum dose and formulation of in enhancing on the level of IgG. It’s therefore expected that Cantella asiatica L.Urban can be used as an immunomodulatory suplement in both animals and human.Methods: Centella asiatica L.Urban were macerated with ethanol 50%. This was an experimental study with the post-test control group design. The samples in this study were 35 Wistar strain male mices which divided into 7 groups: negative control [Aquadest 0,5mL/20g BW]; control immunosupresant [Prednison® 0,06mg/20g BW]; control immunostimulant [Levamisol® 0,445mg/20g BW]; ethanolic extract of Cantella asiatica L.Urban with a dose of 50mg; 100mg; 150mg; 200mg/Kg B.W were administered orally fo 10 day, after the treatments implemented, BCG [Bacillus Calmette Guerin] vaccine was infected intraperitoneally on day 10th, 14th, 21st, and 28th. The most effective doses of ethanolic extract of Cantella asiatica L.Urban was further studied for its effect on hormonal assay using ELISA reader which compared with the effects of Prednison® and Levamisol®. Based on simplex lattice design to find the optimum proportion of coefficients a, b, and ab of the equation Y = a (A) + b (B) + ab (A) (B), where A is lactose and B is amylum.Results : Oral administration of ethanolic extract of Cantella asiatica L.Urban at dose of 50mg and 100mg/Kg B.W increased the immunity which showed elevated levels of IgG in the blood serum in Wistar strain male mices that had been induced by BCG vaccine. The optimum formula capsul of Centella asiatica L.Urban extract that use combination of 80% Amylum and 20% Lactose which produce the optimum parameters of disintegration time (≤15minutes) and CV of weight uniformity (≤5%)with the test results had no significant difference to the prediction results of Simplex Lattice Design [p>0.05].Keywords:  Centella asiatica L.Urban extract, Immunomodulator, simplex lattice design
UJI AKTIVITAS IMUNOSTIMULATOR FRAKSI AIR DARI EKSTRAK ETANOL KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) TERHADAP PROLIFERASI SEL LIMFOSIT MENCIT GALUR SWISS SECARA IN VITRO BESERTA IDENTIFIKASI KANDUNGAN KIMIANYA Puspitowati, Oktarina Heni; Ulfah, Maria; Sasmito, Ediati
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Jurnal Ilmu Farmasi & Farmasi Klinik Vol.9 No.2 Desember 2012
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACTRoselle calyx has been used as traditional medicine for generations. The content of phenols and flavonoids in the roselle calyx thought to have immunostimulatory effects. This study aims to determine the immunostimulatory activity in vitro and to identified phenolic and flavonoids compounds in the water fraction of roselle (Hibiscus sabdariffa L.) calyx ethanol extract.Powder of roselle calyx macerated with 96% ethanol. The extract were obtained partitioned gradually with n-hexana, ethyl acetate, and water. Immunostimulatory activity test was done according to MTT Assay with series of water fraction at concentration 10, 20, 50,100, 200, 400 μg/mL and positive control PHA 10 μg/ml against to lymphocyte cell culture. Optical Density (OD) data was statistically analyzed by Oneway ANOVA followed Tukey test (p<0.05). Identification of phenolic and flavonoids compounds in roselle calyx done by Thin Layer Chromatography (TLC).The results of statistical analysis showed that water fraction have immunostimulatory activity against lymphocyte proliferation at a concentration of 400 μg/mL were significantly different compared to the positive control and the cells control (p<0.05). TLC test results showed that the fraction contained phenols and flavonoids.Key words : roselle (Hibiscus sabdariffa L.) calyx, MTT Assay, immunostimulatory, phenolic and flavonoid.
UJI AKTIVITAS IMUNOSTIMULATOR FRAKSI ETIL ASETAT EKSTRAK ETANOL KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) TERHADAP PROLIFERASI SEL LIMFOSIT MENCIT GALUR SWISS SECARA IN VITRO BESERTA IDENTIFIKASI KANDUNGAN SENYAWA KIMIANYA Ulfah, Maria; Nirmalasari, Nirmalasari; Sasmito, Ediati
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Jurnal Ilmu Farmasi & Farmasi Klinik Vol.10 No.1 Juni 2013
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACTImmunostimulatory agent has an important role in the treatment of infectious disease caused bypathogen. Roselle calyx (Hibiscus sabdariffa  immune system. The aims of this research was to know immunostimulatory activity of ethyl acetate fraction ofethanolic extract of roselle calyx on lymphocyte cell proliferation swiss strain mice and to identified thechemical compounds of the fraction.Roselle calyx was ext racted by maceration method using ethanol 96% as a solvent. The ethanolicextract was partitioned gradually by n-hexan and ethyl acetate. Series of ethyl acetate fraction were made toconcentration 10, 20, 50, 100, 200, 400 μg/mL and positive control PHA 10 μg/mL for tested to lymphocyte cellculture. Immunostimulatory act ivity test was done according to MTTAssay  Key words : roselle calyx, ethyl acetate fraction, MTTAssay , immunostimulatorymethod. Lymphocyte proliferat ion activity was analyzed statistically to the value of Optical Density (OD) result of the Enzyme LinkedImmunosorbent Assay (ELISA) reader using Kruskal Wallis Test continued by Mann Whitney Test (p<0,05).Identificat ion of the contents phenolic compounds and flavonoids performed by Thin Layer Chromatography(TLC).The result of the research showed that ethyl acetate fraction had lymphocyte cell proliferation activity atthe concentration of 50, 100, 200, and 400 μg/mL. The ethyl acetate fraction of roselle calyx contained phenoliccompounds and flavonoids.L.) used by community as a health drink to enhance body’s
UJI AKTIVITAS IMUNOMODULATOR FERMENTASI TEH HITAM JAMUR KOMBUCHA TERHADAP ROLIFERASI SEL LIMFOSIT MENCIT GALUR BALB/C SECARA IN VITRO Qodriyan Sofiakmi, Rahma Laila; Ulfah, Maria; Sasmito, Ediati
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Prosiding Seminar Nasional "Perkembangan Terbaru Pemanfaatan Herbal Sebagai Agen Preventif Pada Ter
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRAK Teh kombucha diperoleh dari hasil fermentasi larutan teh, gula dan koloni kombucha.  Fermentasi teh hitam jamur kombucha (FTHJK) secara empiris, terbukti dapat meningkatkan sistem kekebalan tubuh. Penelitian ini bertujuan untuk mengetahui aktivitas imunomodulator hasil FTHJK terhadap proliferasi sel limfosit mencit galur Balb/C secara in vitro. Kombucha difermentasi selama 5, 10, dan 15 hari dengan menggunakan seduhan teh hitam yang telah diberi gula. Hasil fermentasi teh hitam jamur kombucha (FTHJK) dibuat beberapa seri konsentrasi yakni 6,25; 12,5; 25; 50 dan 100 µg/ml dan sebagai kontrol positif Phytohemagglutinin (PHA) 10 µg/ml diuji secara in vitro terhadap proliferasi sel limfosit mencit galur Balb/C jantan usia 2 bulan dengan menggunakan metode MTT Assay. Data absorbansi berupa nilai Optical Density (OD) dianalisis secara statistik dengan Friedman Test dilanjutkan Mann Whitney Test. Hasil penelitian menunjukkan bahwa FTHJK memiliki aktivitas imunostimulator terhadap proliferasi sel limfosit pada konsentrasi 50 dan 100µg/mL fermentasi hari ke-5, dan konsentrasi 100µg/mL fermentasi hari ke-10 dan 15. Aktivitas imunostimulator tertinggi terjadi pada konsentrasi 100µg/mL dengan lama fermentasi hari ke-15. kata kunci: Teh hitam jamur kombucha, imunomodulator, MTT Assay,Optical Density (OD).
EFEK PENGHAMBATAN EKSTRAK N-HEKSANA BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP REAKSI ANAFILAKSIS KUTANEUS AKTIF PADA TIKUS WISTAR YANG DIINDUKSI VAKSIN HEPATITIS B Sasmito, Ediati; Nugroho, Agung Endro; V. Sagala, Yose
Majalah Obat Tradisional Vol 19, No 1 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/mot-TradMedJ19iss1pp%p

Abstract

Mengkudu (Morinda citrifolia L.) adalah salah satu tanaman berkhasiat obat  yang sudah tidak asing lagi penggunaannya dalam pengobatan. Mengkudu telah diketahui memiliki efek farmakologis, antara lain efek analgesik, antihipertensi, dan imunomodulator. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak n-heksana buah mengkudu dapat menghambat reaksi anafilaksis kutaneus aktif pada tikus Wistar jantan yang diinduksi vaksin hepatitis B. Penelitian ini menggunakan metode anafilaksis kutaneus aktif, kemudian dilanjutkan dengan pengamatan histopatologinya. Uji anafilaksis kutaneus aktif dilakukan selama dua minggu. Hewan uji dibagi menjadi lima kelompok yaitu kelompok : kontrol tanpa perlakuan, perlakuan dengan  ekstrak n-heksana buah mengkudu dosis   50mg, 100mg, dan 200mg/kgBB tikus, dan kelompok yang diberi kromolin dosis 2,16mg/kg BB tikus sebagai kontrol positif. Hewan uji disensitisasi 2 kali (1x setiap minggu) dengan vaksin hepatitis B (dosis 145µg/kgBB tikus) secara subkutan pada bagian punggung. Pada minggu kedua, hewan uji diambil secara acak 4 ekor setiap kelompok untuk dikurbankan dan diambil jaringan kulit beserta jaringan hatinya. Jaringan dibuat sebagai preparat histopatologi dengan pewarnaan hematoxyllin-eosin. Hasil penelitian menunjukkan bahwa pemberian ekstrak n-heksana buah mengkudu dosis 50mg, 100mg, dan 200mg/kgBB pada tikus jantan Wistar yang diinduksi vaksin hepatitis B dapat menghambat reaksi anafilaksis kutaneus aktif.
UJI AKTIVITAS IMUNOSTIMULATOR FRAKSI ETIL ASETAT EKSTRAK ETANOL KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) TERHADAP PROLIFERASI SEL LIMFOSIT MENCIT GALUR SWISS SECARA IN VITRO BESERTA IDENTIFIKASI KANDUNGAN SENYAWA KIMIANYA Nirmalasari, Nirmalasari; Ulfah, Maria; Sasmito, Ediati
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Jurnal Ilmu Farmasi & Farmasi Klinik Vol.10 No.1 Juni 2013
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACTImmunostimulatory agent has an important role in the treatment of infectious disease caused by pathogen. Roselle calyx (Hibiscus sabdariffa L.) used by community as a health drink to enhance body’s immune system. The aims of this research was to know immunostimulatory activity of ethyl acetate fraction of ethanolic extract of roselle calyx on lymphocyte cell proliferation swiss strain mice and to identified the chemical compounds of the fraction. Roselle calyx was extracted by maceration method using ethanol 96% as a solvent. The ethanolic extract was partitioned gradually by n-hexan and ethyl acetate. Series of ethyl acetate fraction were made to concentration 10, 20, 50, 100, 200, 400 μg/mL and positive control PHA 10 μg/mL for tested to lymphocyte cell culture. Immunostimulatory activity test was done according to MTT Assay method. Lymphocyte proliferation activity was analyzed statistically to the value of Optical Density (OD) result of the Enzyme Linked Immunosorbent Assay (ELISA) reader using Kruskal Wallis Test continued by Mann Whitney Test (p<0,05). Identification of the contents phenolic compounds and flavonoids performed by Thin Layer Chromatography (TLC). The result of the research showed that ethyl acetate fraction had lymphocyte cell proliferation activity at the concentration of 50, 100, 200, and 400 μg/mL. The ethyl acetate fraction of roselle calyx contained phenolic compounds and flavonoids. Key words : roselle calyx, ethyl acetate fraction, MTT Assay, immunostimulatory
UJI AKTIVITAS IMUNOMODULATOR FERMENTASI TEH ROSELA JAMUR KOMBUCHA TERHADAP PROLIFERASI SEL LIMFOSIT MENCIT GALUR BALB/C SECARA IN VITRO Ulfah, Maria; Octaviani, Dinar Putri; Sasmito, Ediati
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Jurnal Ilmu Farmasi & Farmasi Klinik Volume 12 No.2 Desember 2015
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACT Recently, some research showed that there is an immunomodulator effect in the fermentation of Rosella kombucha mushroom  tea and it can be caused by flavonoid and organic acid. The purpose of this research was to show the activity of immunomodulator Rosella kombucha mushroom  tea to against the proliferation of lymphocyte cells of Balb/C mice in vitro and to show the dose and length of fermentation that induces highest immunomodulator activity.  Kombucha mushroom was fermented for 5 days, 10 days and also 15 days with boiled Rosella tea, water and sugar. The yield of fermentation in the concentration of 6,25; 12,5; 25; 50 and 100 µg/mL were tested on lymphocyte cells proliferation by the MTT Assay. The OD (Optical Density) data obtained was  statistically analyzed by nonparametric Friedman test and continuoused by Mann-Whitney test. The result of this research showed that the fermentation of Rosella kombucha mushroom tea  has an immunostimulatory activity on lymphocyte cells proliferation with the concentration 6,25; 12,5 and 25 µg/mL in the 10 days and the highest immunostimulatory activity  showed by the concentration 25 µg/mL and 10 days of fermentation time. Key words: immunomodulatory, optical density, Rosella kombucha mushroom tea.