Articles

Found 4 Documents
Search
Journal : Journal of Aquaculture Management and Technology

Pengaruh Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila Syahida, Intan Eska Amalia; Sarjito, Sarjito; Prayitno, Slamet Budi; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Original Source | Check in Google Scholar

Abstract

The study aimed was to know the influence of Piper crocatum exstract on the blood profile included total erythrocyte, total leukocyte, differential leukocyte (DL), phagocytosis indeks (IF) and survival rate of common carp (Cyprinus carpio) infected by Aeromonas hydrophila and the best concentration of P. crocatum for imunostimulatory common carp of infected A. hydrophila. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The methods used in this research is experimental methods by using random design complete (RAL) with 4 treatments and three replicates. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The treatments of different addition of P. crocatum exstract on fish feed, 0 gr/kg feed (treatment A), 5 gr/kg feed (treatment B), 10 gr/kg feed (treatment C), 15 gr/kg feed (treatment D). The treatnent was given 21 days. The research results obtained additional P. crocatum exstract significantly different effect (P0,05) on percentage of netrofil (2%) and survival rate of common carp (C. carpio) infected by A. hydrophila (40%), the best concentration of P. crocatum on feed for immunostimulant common carp of infected A. hydrophila, the result showed the best dose 5 g/kg feed
Pengaruh Ekstrak Daun Sirsak (Annona muricata) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila Nurjannah, R. Dewi Dharina; Prayitno, Slamet Budi; Sarjito, Sarjito; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Original Source | Check in Google Scholar

Abstract

Carp is a freshwater species that is easily cultivated. One of the constraints in cultivating carps (C. carpio) is diseases that ultimately caused mass mortality. The disease that generally affects carp is Motile Aeromonas Septicemia (MAS) caused by Aeromonas hydrophila. Soursop leaves is one of the herbs that can be used as an ingredient to control fish diseases because it contains chemicals that acts bacteriocide. The purpose of this research was to determine the influence and the best dose of soursop leaf extract in feed to the blood profile and survival of carp infected by A. hydrophila. The treatments of this research were addition of soursop leaf extract on feed with 4 different dosages and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet) and D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B (5 g/kg diet) significantly different (P0.05) on total erythrocyte (0.83×106 cells/mm3), differential leukocyte include the percentage of monocytes (7.67%), neutrophils (8.67%), lymphocytes (83.67%), phagocytic activity (37%) and survival rate (50%). It can be conclude that soursop leaf extract at dosage 5g/kg was able to stimulate immune response of carp, it was characterized by an increased total leukocytes of carp infected by A. hydrophila.
Pengaruh Penggunaan Ekstrak Daun Jeruju (Acanthus Ilicifolius) Dengan Dosis Berbeda Terhadap Gambaran Darah, Gejala Klinis Dan Kelulushidupan Lele Dumbo (Clarias gariepinus) Yang Diinfeksi Aeromonas hydrophila Ziyadaturrohmah, Siti; Prayitno, Slamet Budi; Sarjito, Sarjito; Hidayati, Nurul; Saptiani, Gina
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Original Source | Check in Google Scholar

Abstract

Aeromonas hydrophila is a causative agent of Haemoragic Septicemia on Clarias gariepinus. The aim of this research was to investigate the effect of Acanthus ilicifolius leaf extract toward survival rate of C. gariepinus infected by A. hydrophila. Tested fish C. gariepinus 10-12 cm and weight ±25 gr. This reasearch was conducted by 4(four) treatments namely, A (treatment with no leaf ectract A. ilicifolius), B (300 ppm), C (500 ppm), and D (700) 30 minutes immersion. The challenge test was done by injecting 0,1 mL A. hydrophila suspensions with dosage 108 cell/mm3 intra-muscular by experiment fishes. Observation was performed for 5 days after infection such as blood profile, clinical symptoms, and survival rate of C. gariepinus. The results of blood profile after the infection showed that hematokrit of A, B, C, and D treatments was 17,33%, 14,67%, 15,00%, 19,67% respectively. Percentage of the highest erythrocyte was at D treatment 1,79x106 sel/mm3, B 1,72 x106 sel/mm3, A 1,64x106 sel/mm3, C 1,52x106 sel/mm3. Percentage of the highest leuchocyte was at C treatment 5,43x104 sel/mm3, D 3,11x104 sel/mm3, B 2,63x104 sel/mm3, and A 2,32x104 sel/mm3. The result showed that clinical symptoms of A. hydrophila infected fish swam abnormally, injured and haemoragic on the skin along with damaged on the body. Immersion with A. ilicifolius extract leaf past infection indicated that they were not significantly different on C. gariepinus survival rate. Therefore the dosage of A. ilicifolius leaf extract did not sufficient to protect C. gariepinus from A. hydrophila infection.
Pengaruh Ekstrak Daun Sirsak (Annona muricata L) Terhadap Profil Darah dan Kelulushidupan Ikan Lele Sangkuriang (Clarias gariepinus Var. Sangkuriang) yang Diinfeksi Bakteri Aeromonas Hydrophila Kurniawan, Kurniawan; Prayitno, Slamet Budi; Sarjito, Sarjito; Lusiastuti, Angela Mariana
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Original Source | Check in Google Scholar

Abstract

The main problem in catfish farms is mass mortality coused by Motile Aeromonas Septicemia (MAS) by Aeromonas hydrophila. Natural ingredients that are currently being developed is soursop leaf. It was because soursop leaf contains acetogenin compounds that wishes compared to other plant parts. An acetogenin compound has the benefit of as an antibacterial. The purpose of this research was to determine the influence and best dose of sourshoup leaf extract in feed to the blood profile and survival rate of catfish infected by A. hydrophila. The treatment in this study were the addition of soursop leaf extract on feed with different dosage and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet), D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B on the best dose (5 g/kg diet) was total erythrocytes(1,51 x 106 sel/mm3), total leukocytes (8,00 x 103 sel/mm3), limphocytes (92%), monocyte (8%), neutrophils, phagocytic index (75%), and survival rate (93%). It can be concluded that dose of 5 g/kg diet was the best dosage of soursop leaf extract in the feed that was able to stimulate the immune response of catfish, it was characterized by an increased erythrocytes, total leukocytes, differetial leukocyte (limphocytes and monocyte) of catfish infected by A. hydrophila.