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Penggunaan Repetitive Sequence-Based Polychain Reaction (REP-PCR) Untuk Pengelompokan Bakteri Vibrio yang Berasosiasi dengan Ikan Kerapu Sakit dari Perairan Karimunjawa

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Ikan kerapu sakit diperoleh dari keramba jaring apung yang berlokasi di perairan Karimunjawa. Penelitian ini bertujuan  untuk  mengkaji  penggunaan  Repetitive  Sequence-Based  Polychain  Reaction (REP-PCR)  untuk pengelompokan bakteri genus vibrio yang berasosiasi dengan berbagai ikan kerapu sakit.  Sebanyak 32  isolat Vibrio berhasil diisolasi dari bagian luka maupun ginjal berbagai ikan kerapu sakit dengan  medium Thiosulfat Citrat Bile Salt Agar (TCBSA).  Hasil rep-PCR diperoleh bahwa terdapat delapan kelompok bakteri vibrio yang berasosiasi dengan ikan kerapu sakit.  Oleh karena itu,  pada penelitian ini, delapan  isolat (JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, dan JT 31) yang masing-masing mewakili kelompoknya akan dilakukan uji selanjutnya. Teknik  molekuler  gen 16S  rDNA  digunakan  untuk  karakterisasi  kedelapan  isolat    secara  komprehensif. Berdasarkan analisis sekuen gen 16S rDNA, data menunjukkan bahwa isolat JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, dan JT 31 memiliki kekerabatan terdekat dengan Vibrio natriegens, V. olivaceus,  V. damsella ATCC33, V. fortis, V. alginolyticus, V. harveyi, V. parahaemolyticus and V. carcharieae.  Dari hasil penelitian ini dapat disimpulkan bahwa rep-PCR dapat digunakan untuk pendekatan molekuler secara efisien pada bakteri vibrio yang berasosiasi dengan kerapu sakit.Kata kunci: rep-PCR, Vibrio, Kerapu, Karimunjawa Moribound grouper fish was taken from the cages located in Karimunjawa waters. The research aim was to apply Repetitive Sequence-Based Polychain Reaction (REP-PCR) to group  the vibriosis on groupers from Karimunjawa waters.  Thirty two  isolates of Vibrio were isolated from external wound and kidney of groupers.  Based on the rep-PCR results found  eight  groups of vibrio bacteria associated with moribund groupers fish. Eight isolates, i.e. isolate of JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, and JT 31 were continued to characterize using the molecular techniques of 16S rDNA. Based on the results of sequen analysis, data showed that isolate of JT 02, JT 07, JT 10, JT 13, JT 20, JT 24, JT 27, and JT 31 was closely related to Vibrio natriegens, V. olivaceus,  V. damsella ATCC33, V. fortis, V. alginolyticus, V. harveyi, V. parahaemolyticus and V. carcharieae, respectively . The present research concluded that rep-PCR was able to conduct biomoleculer approach efficiently for vibrios bacteria in moribund groupers fish. Key words: rep-PCR, Vibrio, groupers, Karimunjawa

Fenomena Pertumbuhan Compensatory dan Kualitas Ikan Nila Merah (Oreochromis sp.) pada Kondisi Laut

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 2 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Penelitian skala laboratorium untuk mengevaluasi fenomena pertumbuhan compensatory dan kualitas produk (body composition) dari nila merah (Oreochromis sp.) yang dipelihara di air laut dilakukan pada awal Juni sampai awal Agustus 2003 di Hatchery Kampus Kelautan Teluk Awur Jepara FPIK UNDIP. Benih ikan (37,74gr + SD 1,16gr) sebelumnya diaklimatisasikan pada kondisi laut dan dipelihara dalam bak-bak percobaan dengan kepadatan 5 ekor/m3. Perlakuan pemuasaan dengan 3 kali ulangan selama 4 minggu percobaan, yaitu: ikan diberi pakan setiap hari (A/kontrol); diberi pakan selama 6 hari diikuti pemuasaan 1 hari (B); diberi pakan selama 5 hari diikuti pemuasaan 2 hari (C); dan, diberi pakan selama 4 hari diikuti pemuasaan 3 hari (D). Pakan berbentuk pellet tenggelam (PT CP Prima) dengan kandungan protein 24-26%, lemak 3-5%, serat kasar 4-6%, abu 5-8% dan air 11-13%, diberikan 2 kali sehari sebanyak 5% dari biomassa. Pengamatan pertumbuhan dilakukan seminggu sekali. Analisa body composition dilakukan untuk mengukur kandungan protein, lemak, karbohidrat dan air (%). Hasil penelitian memperlihatkan bahwa tidak terjadi perbedaan dalam pertumbuhan selama percobaan; hal ini menunjukkan terjadi fenomena pertumbuhan compensatory. Tingkat pertumbuhanpada masing-masing perlakuan yaitu 7,42 gr/minggu (A); 7,18 gr/minggu (B); 3,44 gr/minggu (C); dan, 5,34 gr/minggu (D). Analisis body composition tidak menunjukkan adanya perbedaan dalam kandungan protein, lemak, karbohidrat, maupun air. Kandungan protein normal (>18%). Kandungan lemak rendah, 1,50-1,80%, diikuti kandungan air yang tinggi (>70%), yang menyebabkan tekstur daging lebih lunak. Kandungan karbohidrat 2,7-4,8%.Kata kunci: nila merah, pertumbuhan compensatory, kuantitas, kualitasThe study to evaluate both compensatory growth phenomenon occurred and the fish quality (body composition) were done under the laboratory conditions from beginning June to beginning August 2003 at the hatchery of Marine Science Teluk Awur-Jepara Campus, Diponegoro University. Red tilapias of mean weight of 37.74g+SD 1.16 g were acclimated in seawater conditions. The fish were cultured in the tank with a density of fivefish/m3 The treatments were feeding daily (A/control); fish fed 6 days–a day unfed (B); fish fed 5 days-2 days unfed; and fish fed 4 days-3 days unfed (D). The food was slowly sinking type (CP Prima) containing protein24-26%, oil 3-5%, fibre 4-6%, ash 5-8%, and water 11-13%. Feeding frequency was twice a day with 5% of the biomass. Growth was measured weekly. For fish quality analysis (body composition), it was done tomeasure the contents of protein, fat, carbohydrate, and water (%). The result showed that there was no significant difference of the growth among the fish (ANOVA); and, in turn, it suggested that the compensatorygrowth was occurred. The growth rates were 7.42 g/week (A), 7.18 g/week (B), 3.44 g/week(C), and 5.34 g/week (D). There was no difference for the contents of protein, fat, carbohydrate, and water. In general, theprotein content was above 18%.The low fat contents (1.50-1.80%) was followed by the high water contents (>70%), so that it made the flesh texture being soft. The carbohydrate contents were 2.74 to 4.8%.Key words: red tilapia, compensatory growth, quantity, quality

Causative Agent Vibriosis dari Kerapu Bebebk (Cromileptis altivelis ) : 2. Kakarkterisasi secara Molekuler Berbasis 16 S rDNA

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 4 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Ikan Kerapu Bebek (Cromileptis altivelis) sakit diperoleh dari keramba jaring apung di Karimunjawa. Penelitian ini bertujuan untuk mengkaji causative agent utama vibriosis pada ikan kerapu bebek  (C. altivelis) dari  karamba jaring apung di perairan Karimun Jawa.  Sebanyak tujuh isolat vibrio diisolasi dari bagian luka maupun ginjal kerapu bebek sakit yang menunjukkan gejala vibriosis.  Hasil uji postulat koch dan pathogenisitasnya dari enam isolat,  diperoleh bahwa tiga isolat (isolat  JT 07; JT 10 dan JT 20 ) yang mengakibatkan 100% dan merupakan agensia penyebab utama vibriosis pada ikan Kerapu Bebek . Oleh karena itu,  pada penelitian ini hanya tiga isolat ini yang akan dilakukan uji selanjutnya. Teknik  molekuler gen 16S rDNA (amplifikasi 16S DNA ribosom) digunakan untuk karakterisasi ketiga causative agent utama secara komprehensif. Berdasarkan analisis sekuen gen 16S rDNA, data menunjukkan bahwa isolat JT 07 memiliki kekerabatan terdekat dengan Vibrio olivaceus (99%); isolat JT 10 dengan  V. damsella (99%)  dan isolat JT 20  dengan  V. alginolyticus  (98%). Kata kunci:  causative agent, Vibriosis,  molekuler,  Kerapu bebekMoribound Humpbeck Grouper fish (Cromileptis altivelis) was taken from the cages of Karimunjawa. The research aim was to find out the main causative agent of vibriosis on humpbeck grouper (C. altivelis)  from Karimunjawa waters. Seven  isolates of Vibrio were isolated from external wound and kidney of the humpbeck grouper fish (C. altivelis) which showed the clinical signs of vibriosis. Based on the koch postulate and pathogenecity test results indicated that three vibrios  (isolate JT 07; JT 10 and JT 20 ) act as a main causative agents of vibriosis which caused mortality of  100% to E. fuscogutatus.  Because of highest mortality as pathogenicity indicator, the three isolates were continued to investigate. A complementary molecular techniques of 16S rDNA genes (amplified 16S ribosomal DNA) was used to give a comprehensive characterization of these isolates.  On the basis of the results of sequen analysis, our data showed that JT 07; JT 10 and JT 20 isolates were closely related to Vibrio olivaceus (99.0%); V. damsella (99%)  and  V. alginolyticus  (98%) respectively.. Key Words : Causative agent, Vibriosis, molecular,  humpbeck grouper  

Causitive Agent Vibriosis dari Ikan Kerapu Bebek (Cromileptis altivelis) Bermulut Merah : 1. Patogenitas pada Ikan Kerapu Macan (Epinephelus fuscoguttatus)

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 3 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

The research aims were to find out the causative agent vibriosis of Cromileptis altivelis  having clinical symptom red mouth and its pathogenecity to Epinephelus fuscoguttatus.   Seven isolats Vibrio were isolated from wound and kidney of  C.  Altivelis.  The result of Koch postulate indicated that five vibrios as a causative agent of vibriosis, consisted of three vibrios (isolat JT 07,   JT 10, JT 20) and  two vibrios (isolat JT 4,  JT 29) caused  mortality of 100% and 40% on E. fuscogutatus respectively.  Three isolat vibrios ( JT 7,   JT 10, JT 20 ) with higher pathogenicity were continued to futher investigation.  Mean time to death of  V. fuscus (JT 07), V. alginolyticus and V. anguillarum, to E. fuscogutatus  on concentration of 108 CFU/mL  were 83,33%  (11,25 hours); 79,16%(15,63 hours); dan 50% (20,5 hours) respectively; whereas  on concentration of 109 CFU/mL were 95,83% (10,8 hours); 87,5%(15,28 hours); dan 62,5% (19,6 hours) respectively. Lethal Concentration Median (LC50) of V. Fuscus, V. alginolyticus,  V. anguillarum were  3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL. All isolates on concentration of 106 dan 107 CFU/mL did not cause 50%  tested fish mortality Key words : Causative agent, Vibriosis, E. Fuscogutatus,  V. parahaemolyticus, Pathogenicity Penelitian ini bertujuan untuk mengkaji causative agent  vibriosis pada ikan Kerapu Bebek (Cromileptis altivelis) dengan gejala klinis mulut merah serta patogenisitasnya terhadap ikan Kerapu Macan (Epinephelus fuscoguttatus).  Tujuh  isolat Vibrio berhasil diisolasi dari bagian luka dan ginjal Kerapu Bebek Mulut Merah.  Hasil uji postulat koch memperlihatkan lima isolat dimana isolat  JT 07,   JT 10, JT 20 dapat mengakibatkan kematian 100%, sedangkan isolat  JT 04 dan JT 29 menyebabkan kematian 40%. Pada tiga isolat (Vibrio JT 07, JT 10 dan JT 20) yang memperlihatkan patogenitas yang lebih tinggi dilakukan uji lanjutan.  Hasil karakterisasi melalui uji morfologi dan biokimia diperoleh bahwa isolat JT 07 memiliki kemiripan 96,15 % dengan Vibrio fuscus;  JT 10 memiliki kemiripan 100% dengan Vibrio anguillarum dan JT 20 memiliki kemiripan 100% dengan Vibrio alginolyticus. Patogenisitas ketiga isolat vibrio tersebut secara berurutan adalah V. fuscus, V. alginolyticus,  V. anguillarum, dimana diperoleh bahwa  persentase kematian dan rerata waktu kematian (Mean Time to Death, MTD) pada penyuntikan intraperitoneal V. fuscus (JT 07), V. alginolyticus (JT 20) dan V. anguillarum (JT 10) dengan konsentrasi 108 CFU/mL adalah berturut-turut 83,33%  (11,25 jam); 79,16% (15,63 jam); dan 50% (20,5 jam); sedangkan untuk konsentrasi 109 CFU/mL secara berurutan adalah 95,83% (10,8 jam); 87,5% (15,28 jam); dan 62,5% (19,6 jam). Lethal Concentration Median (LC50) V. fuscus,  V.  alginolyticus,  V.  anguillarum secara berurutan adalah  sebesar  3,2X107 CFU/mL; 4,8 X 108 CFU/mL; dan 2,24X108 CFU/mL.  Sedang pada konsentrasi 106 dan 107 CFU/mL semua isolat tidak menimbulkan kematian pada ikan uji.  Hasil ini menunjukkan bahwa tiga causative agent tersebut bersifat patogen pada ikan kerapu. Kata kunci:  Causative agent, Vibriosis, E. fuscogutatus V. parahaemolyticus, Patogenisitas

ANALISA KEKUATAN PUNTIR, LENTUR PUTAR DAN KEKERASAN BAJA ST 60 UNTUK POROS PROPELLER SETELAH DIQUENCHING

ROTASI "JURNAL TEKNIK MESIN" Volume 11, Nomor 2, April 2009
Publisher : ROTASI "JURNAL TEKNIK MESIN"

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Abstract

In the propeller rotation to obtain the ship thrust, the shaft occurred the vary of pressures as the effect of the force combination. The pressures continuously happened and it cause fatique on the material. Therefore the heat treatment occurred to produce high destructive resistance and to gain the good strength. In this research thr heat treatment ic processed by Quenching, slight cooling and used oil as the cooling media.This research objected to know the mechanical and physical type of material such as ultimate strength, yield strength, twisting, rotating bending, hardness and the composition of ST 60 stell after being Quenching will be compared to the ST 60 stell without Quenching, those material used as the propulsion material. The material was ST 60 stell, contained carbon 0,35-0,4% C. The test occurred for both type, they are physical type (composition) and mechanical type ( hardness, ultimate strength, twisting and rotating bending). The spesiment being heat at the temperature of 850º C during 20 menit and being oiled Quenching, then the test of bending, twisting, rotating bending, composition and hardness are occurred.In accordance to the research. The ultimate strength ST 60 after being Quenched is 860, 27 N/mm², yield strenght is 695,95 N/mm², the Brinell hardness is 263,209, the material compositions are 0,5% C, 0,75% Mn, 0,34% Si, 0,0098% P, 0,0009% S, rotating bending strenght is 337,08 Mpa with the 1.298.600 sicluses and twisting strength is 21,07 Mpa.

Pengaruh Temperatur dan Photoperiod Terhadap Kematangan Gonad Kepiting Bakau (Scylla serrata)

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 2 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Penelitian dilakukan untuk mengetahui pengaruh photoperiod dan temperatur terhadap pematangan gonad kepiting bakau. Penelitian dilaksanakan di Hatchery Marine Center IImu Kelautan Jepara, dari bulan September 1999 sampai dengan bulan Desember 1999. Metoda penelitan menggunakan rancangan acak lengkap (RAL) faktorial faktor pertama adalah perlakuan photoperiod dengan 2 taraf pelakuan. yaitu short­day dan long-day. Sedangkan faktor kedua adalah perlakuan temperatur yang juga mempunyai 2 taraf perlakuan yaitu 29 oC dan 31 oC. Hasil penelitian menunjukksn bahwa perlakuan dengan kombinasi photoperiod dan temperatur berpengaruh terhadap perkembangan gonad kepiting bakau. Urutan besarnya nilai lndek kematangan gonad (IKG) adalah 25,41 (A 1B I); 22,06 (A 1B2):20,77 (A2B I) dan 19,88 (A2B2). Hasil analisis regresi nilai simpangan (b) dari persamaan garis menunjukkan nilai IKG terbaik dicapai pada perlakuan short-day dan temperatur 29oC (A 1B1). Sedangkan terendah pada perlakuan long-day dan temperatur 31°C (A2B2).Kata kunci: reproduksi, photoperiod, temperatur; indek kematangan gonad  The research is to investigate the effect of photoperiod and temperature on the gonad development (GSI) of the mud crab. This research was conducted at the marine centre hatchery Jepara from September to December 1999. The method used was completely factorial randomized design. The first factor was photoperiod i.e. short-day and long-day. The second factor was i.e temperetur 29 oC and 31 oC. The result showed that photoperiod and temperatur in combination influenced gonad development of the mud crab. The degree of the gonado somatic index (GSI) was 25,41 (A 1B1); 22,06 (A 1B2); 20,77 (A2B1) and 19.88 (A2B2). The result of regression analysis showed that the highest and the lowest gonado somatic indices occured on the combination between short-day and 29 oC and long-day temperatur 3 1oC respectively. Keywords: reproduction, photoperiod, temperatur; and gonado somatic index (GSI)

Pengaruh Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila

Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

The study aimed was to know the influence of Piper crocatum exstract on the blood profile included total erythrocyte, total leukocyte, differential leukocyte (DL), phagocytosis indeks (IF) and survival rate of common carp (Cyprinus carpio) infected by Aeromonas hydrophila and the best concentration of P. crocatum for imunostimulatory common carp of infected A. hydrophila. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The methods used in this research is experimental methods by using random design complete (RAL) with 4 treatments and three replicates. The research was conducted at Research and Development of Freshwater Aquaculture, Bogor. The treatments of different addition of P. crocatum exstract on fish feed, 0 gr/kg feed (treatment A), 5 gr/kg feed (treatment B), 10 gr/kg feed (treatment C), 15 gr/kg feed (treatment D). The treatnent was given 21 days. The research results obtained additional P. crocatum exstract significantly different effect (P0,05) on percentage of netrofil (2%) and survival rate of common carp (C. carpio) infected by A. hydrophila (40%), the best concentration of P. crocatum on feed for immunostimulant common carp of infected A. hydrophila, the result showed the best dose 5 g/kg feed

Pengaruh Ekstrak Daun Sirsak (Annona muricata) Terhadap Profil Darah Dan Kelulushidupan Ikan Mas (Cyprinus carpio) Yang Diinfeksi Bakteri Aeromonas hydrophila

Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Carp is a freshwater species that is easily cultivated. One of the constraints in cultivating carps (C. carpio) is diseases that ultimately caused mass mortality. The disease that generally affects carp is Motile Aeromonas Septicemia (MAS) caused by Aeromonas hydrophila. Soursop leaves is one of the herbs that can be used as an ingredient to control fish diseases because it contains chemicals that acts bacteriocide. The purpose of this research was to determine the influence and the best dose of soursop leaf extract in feed to the blood profile and survival of carp infected by A. hydrophila. The treatments of this research were addition of soursop leaf extract on feed with 4 different dosages and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet) and D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B (5 g/kg diet) significantly different (P0.05) on total erythrocyte (0.83×106 cells/mm3), differential leukocyte include the percentage of monocytes (7.67%), neutrophils (8.67%), lymphocytes (83.67%), phagocytic activity (37%) and survival rate (50%). It can be conclude that soursop leaf extract at dosage 5g/kg was able to stimulate immune response of carp, it was characterized by an increased total leukocytes of carp infected by A. hydrophila.

Pengaruh Penggunaan Ekstrak Daun Jeruju (Acanthus Ilicifolius) Dengan Dosis Berbeda Terhadap Gambaran Darah, Gejala Klinis Dan Kelulushidupan Lele Dumbo (Clarias gariepinus) Yang Diinfeksi Aeromonas hydrophila

Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Aeromonas hydrophila is a causative agent of Haemoragic Septicemia on Clarias gariepinus. The aim of this research was to investigate the effect of Acanthus ilicifolius leaf extract toward survival rate of C. gariepinus infected by A. hydrophila. Tested fish C. gariepinus 10-12 cm and weight ±25 gr. This reasearch was conducted by 4(four) treatments namely, A (treatment with no leaf ectract A. ilicifolius), B (300 ppm), C (500 ppm), and D (700) 30 minutes immersion. The challenge test was done by injecting 0,1 mL A. hydrophila suspensions with dosage 108 cell/mm3 intra-muscular by experiment fishes. Observation was performed for 5 days after infection such as blood profile, clinical symptoms, and survival rate of C. gariepinus. The results of blood profile after the infection showed that hematokrit of A, B, C, and D treatments was 17,33%, 14,67%, 15,00%, 19,67% respectively. Percentage of the highest erythrocyte was at D treatment 1,79x106 sel/mm3, B 1,72 x106 sel/mm3, A 1,64x106 sel/mm3, C 1,52x106 sel/mm3. Percentage of the highest leuchocyte was at C treatment 5,43x104 sel/mm3, D 3,11x104 sel/mm3, B 2,63x104 sel/mm3, and A 2,32x104 sel/mm3. The result showed that clinical symptoms of A. hydrophila infected fish swam abnormally, injured and haemoragic on the skin along with damaged on the body. Immersion with A. ilicifolius extract leaf past infection indicated that they were not significantly different on C. gariepinus survival rate. Therefore the dosage of A. ilicifolius leaf extract did not sufficient to protect C. gariepinus from A. hydrophila infection.

Pengaruh Ekstrak Daun Sirsak (Annona muricata L) Terhadap Profil Darah dan Kelulushidupan Ikan Lele Sangkuriang (Clarias gariepinus Var. Sangkuriang) yang Diinfeksi Bakteri Aeromonas Hydrophila

Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

The main problem in catfish farms is mass mortality coused by Motile Aeromonas Septicemia (MAS) by Aeromonas hydrophila. Natural ingredients that are currently being developed is soursop leaf. It was because soursop leaf contains acetogenin compounds that wishes compared to other plant parts. An acetogenin compound has the benefit of as an antibacterial. The purpose of this research was to determine the influence and best dose of sourshoup leaf extract in feed to the blood profile and survival rate of catfish infected by A. hydrophila. The treatment in this study were the addition of soursop leaf extract on feed with different dosage and 3 replicates. The treatments were A (0 g/kg diet), B (5 g/kg diet), C (10 g/kg diet), D (15 g/kg diet). The result showed that soursop leaf extract in feed of treatment B on the best dose (5 g/kg diet) was total erythrocytes(1,51 x 106 sel/mm3), total leukocytes (8,00 x 103 sel/mm3), limphocytes (92%), monocyte (8%), neutrophils, phagocytic index (75%), and survival rate (93%). It can be concluded that dose of 5 g/kg diet was the best dosage of soursop leaf extract in the feed that was able to stimulate the immune response of catfish, it was characterized by an increased erythrocytes, total leukocytes, differetial leukocyte (limphocytes and monocyte) of catfish infected by A. hydrophila.