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Respons Histopatologik Karsinoma Serviks Uteri Setelah Pemberian Kemoradiasi Iskandar, T Mirza; Mexitalia, Maria; Sarjadi, Sarjadi; Dharmana, Edi; Pramono, Noor
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 3 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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ABSTRACTFactors associated with histopathologic responses of cervical cancer after chemoradiation therapyIntroduction: Cervical carcinoma is the second most common women cancer in the world. A combined of surgical, radiation and chemotherapy is the main cervical carcinoma treatment. The histopathological response is one performance to assess the result of the treatment. The purpose of the study is to analyze the factors of cervical cancer which contributes to the histopathological response after chemoradiation treatment.Methods: The design of the study was a case control, done at Dr. Kariadi Hospital Semarang in 2008. Patients with biopsy-proven cervical carcinoma were entered into the study. The variables of risk factors included the stage of the cancer by FIGO staging, the type and differentiation of the tumour, the anemia and the history of the transfusion and the immunotherapy by BCG vaccine. The histopathological response was assessed after the chemoradiation treatment. Chi-square was used to analyze the risk factors and logistic regression was used for the multivariate analysis.Results: Between April-August 2008, 77 patients finished the chemoradiation treatment. The type of the cancer was squamous cell carcinoma (71.4%) and the stage was III B (81.8%). The staging, type and differentiation of the tumor, and the history of transfusion did not contributes to the histopathological response. After adjustment of other factors, the contribution of anemia to poor histopathological response were 6.25 times (95% CI 1.12-34.90; p=0.037) higher than good histopahological response.Conclusion: Anemia is the risk factor of poor histopathological response of cervical carcinoma after chemoradiation therapy.Keywords: Cervical carcinoma, chemoradiation, histopahological responseABSTRAKPendahuluan: Karsinoma serviks uteri (KSU) merupakan kanker kedua terbanyak pada wanita di seluruh dunia. Sampai saat ini terapi pilihan utama KSU adalah operasi, radiasi dan kemoterapi. Salah satu penilaian keberhasilan terapi adalah dengan respon histopatologik. Tujuan penelitian ini adalah menilai faktor-faktor yang berperan terhadap respons histopatologik setelah terapi kemoradiasi.Metode: Desain penelitian adalah kasus kontrol. Penelitian dilakukan di RSUP Dr. Kariadi Semarang. Kriteria inklusi adalah KSU yang telah menyelesaikan kemoradiasi. Faktor-faktor yang dinilai berperan terhadap respons histopatologi adalah tipe histologis dan diferensiasi tumor, stadium tumor berdasarkan FIGO, anemia dan riwayat transfusi serta pemberian imunoterapi dengan BCG. Analisis statistik menggunakan kai-kuadrat dan regresi logistik.Hasil: Selama periode April-Agustus 2008 terdapat 77 penderita yang menyelesaikan kemoradiasi. Sebagian besar penderita berada pada stadium IIIB (71,4%) dan mempunyai tipe histologi karsinoma sel skuamosa (81,8%). Stadium tumor, tipe histologi dan diferensiasi tumor, riwayat transfusi serta imunoterapi BCG tidak berperan pada respons histopatologik. Setelah memperhitungkan faktor perancu, risiko anemia terhadap respons histopatologi yang jelek adalah 6,25 kali (95% CI 1,12-34,90; p=0,037).Simpulan: Anemia merupakan faktor risiko terjadinya respon histopatologik yang jelek pada penderita karsinoma serviks uterisetelah mendapatkan terapi kemoradiasi.
Correlation of CagA-Positive Strains of Helicobacter pylori with Topographic Distribution and Chronic Gastritis Grading Arinton, I Gede; Samudro, Pugud; Soemohardjo, Soewignjo; Sarjadi, Sarjadi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 8, ISSUE 1, April 2007
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Background: CagA gene is a marker for the presence of Cag pathogenicity island. CagA-positive strains of Helicobacter pylori can identify individuals who have higher risk of developing gastrointestinal diseases. Aim: To discover the correlation of CagA status of Helicobacter pylori with topographic localization of Helicobacter pylori and chronic gastritis grading. Methods: Gastric biopsy specimens were taken from 104 patients. The specimens were obtained from gastric antrum, corpus and incisures for histological and polymerase chain reaction (PCR) studies. The histological chronic gastritis was assessed semi-quantitatively (grades 0-3). The PCR was used for detecting Helicobacter pylori genes and CagA strain. Topographic localization of Helicobacter pylori was classified as gastric antrum and corpus. Results: There were 33 (86.8%) CagA-positive strains of 38 patients with Helicobacter pylori-positive genes. There were no significant differences between topographic localization of Helicobacter pylori - either in the gastric antrum (rho = 0.14, p = 0.40) nor in the corpus (rho = 0.27, p =0.10) and the CagA status of Helicobacter pylori. Conclusion: CagA gene status of Helicobacter pylori does not determine chronic gastritis grading and gastric topographic localization. Keywords: chronic gastritis, cagA gene, Helicobacter pylori, gastric antrum, gastric corpus.
Typhonium flagelliforme decreases tyrosine kinase and Ki67 expression in mice Chodidjah, Chodidjah; Dharmana, Edi; Susanto, Hardhono; Sarjadi, Sarjadi
Universa Medicina Vol 32, No 3 (2013)
Publisher : Faculty of Medicine, Trisakti University

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Background Worldwide, breast cancer is the most frequent cancer in women after lung cancer. Treatments include surgery, radiation, immunotherapy and chemotherapy, but are not effective. Tyrosine kinase and Ki67 protein are markers of proliferation. Typhonium flagelliforme ethanol extract (TFEE) has been shown to inhibit proliferation of Michigan Cancer Foundation-7 (MCF7) cells in culture. The aim of the present study was to examine the effect of administration of TFEE on tyrosine kinase and Ki67 expression in mice. Methods This experimental study using post test randomized design with control group was conducted in 24 tumor-bearing CH3 mice. They were randomly divided into 4 groups, consisting of one control and 3 treatment groups (TI, T2, T3) treated daily for 30 days with 0.2 ml TFEE at dosages of 200, 400, and 800 mg/kgBW, respectively. On day 31 the tumor tissues were collected and their tyrosine kinase and Ki67 expression were levels assessed using ELISA and immunohistochemical staining, respectively. Tyrosine kinase and Ki67 expression levels were analyzed, respectively using Kruskal Wallis test and one-way Anova followed by Bonferroni post hoc test. Results Mean tyrosine kinase level was highest in the control group, followed by T3, T2 and T1 (p=0.019). Mean level of Ki 67 expression was highest in the control group, followed by T2, T3 and T1 (p=0.000). Conclussions Oral administration of TFEE at a dose of 200 mg/kgBW decreases tyrosine kinase levels and Ki 67 expression.
Mangosteen peel extract reduces formalin-induced liver cell death in rats Rohmani, Afiana; Sarjadi, Sarjadi; Winarto, Winarto; Kisdjamiatun, Kisdjamiatun
Universa Medicina Vol 33, No 2 (2014)
Publisher : Faculty of Medicine, Trisakti University

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BACKGROUNDFormalin is a xenobiotic that is now commonly used as a preservative in the food industry. The liver is an organ that has the highest metabolic capacity as compared to other organs. Mangosteen or Garcinia mangostana Linn (GML) peel contains xanthones, which are a source of natural antioxidants. The purpose of this study was to evaluate the effect of mangosteen peel extract on formalin- induced liver cell mortality rate and p53 protein expression in Wistar rats.METHODSEighteen rats received formalin orally for 2 weeks, and were subsequently divided into 3 groups, consisting of the formalin-control group receiving a placebo and treatment groups 1 and 2, which were treated with mangosteen peel extract at doses of 200 and 400 mg/kgBW/day, respectively. The treatment was carried out for 1 week, and finally the rats were terminated. The differences in liver cell mortality rate and p53 protein expression were analyzed.RESULTSOne-way ANOVA analysis showed significant differences in liver cell mortality rate among the three groups (p=0.004). The liver cell mortality rate in the treatment group receiving 400 mg/kgBW/day extract was lower than that in the formalin- control group. There was no p53 expression in all groups.CONCLUSIONSGarcinia mangostana Linn peel extract reduced the mortality rate of liver cells in rats receiving oral formalin. Involvement of p53 expression in liver cell mortality in rats exposed to oral formalin is presumably negligible.
Andrographis paniculata extract induced apoptosis of adenocarcinoma mammae in C3H mice Nugrahaningsih, Nugrahaningsih; Sarjadi, Sarjadi; Dharmana, Edi; Subagio, Hartanto Wahyu
Universa Medicina Vol 32, No 2 (2013)
Publisher : Faculty of Medicine, Trisakti University

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BACKGROUNDApoptosis plays an important role in tumorigenesis. Induction of apoptosis is a strategy for developing cancer therapy. In vitro study found that andrographolide isolated from Andrographis paniculata has anticancer activity by an apoptotic mechanism in cancer cell lines. The aim of the present study was to prove theeffect of Andrographis paniculata extract administered orally on apoptosis of mammary adenocarcinoma in C3H mice.METHODSThis study was of post test randomized control group design. Twenty four C3H mice with transplanted mammary adenocarcinomas were divided into four groups. To three groups Andrographis paniculata extract was administered orally for14 days, at doses of 5, 10 and 15 mg/day, respectively, whereas to the control group no Andrographis paniculata extract was administered. On day 15 the mice were terminated. The mammary adenocarcinomas were examined by the terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) method. The values of the apoptotic index were expressed as mean±SD and analyzed using ANOVA and Pearson’s correlation test.RESULTSThe mean apoptotic index values differed significantly among the experimental groups (p=0.001). The highest value was found in the group receiving Andrographis paniculata extract 15 mg/day, while the lowest was in the control group, the values being significantly correlated (r=0.974).CONCLUSIONSOral administration of Andrographis paniculata extract induced apoptosis in C3H mice with mammary adenocarcinoma
Correlation of CagA-Positive Strains of Helicobacter pylori with Topographic Distribution and Chronic Gastritis Grading Arinton, I Gede; Samudro, Pugud; Soemohardjo, Soewignjo; Sarjadi, Sarjadi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 8, ISSUE 1, April 2007
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: CagA gene is a marker for the presence of Cag pathogenicity island. CagA-positive strains of Helicobacter pylori can identify individuals who have higher risk of developing gastrointestinal diseases. Aim: To discover the correlation of CagA status of Helicobacter pylori with topographic localization of Helicobacter pylori and chronic gastritis grading. Methods: Gastric biopsy specimens were taken from 104 patients. The specimens were obtained from gastric antrum, corpus and incisures for histological and polymerase chain reaction (PCR) studies. The histological chronic gastritis was assessed semi-quantitatively (grades 0-3). The PCR was used for detecting Helicobacter pylori genes and CagA strain. Topographic localization of Helicobacter pylori was classified as gastric antrum and corpus. Results: There were 33 (86.8%) CagA-positive strains of 38 patients with Helicobacter pylori-positive genes. There were no significant differences between topographic localization of Helicobacter pylori - either in the gastric antrum (rho = 0.14, p = 0.40) nor in the corpus (rho = 0.27, p =0.10) and the CagA status of Helicobacter pylori. Conclusion: CagA gene status of Helicobacter pylori does not determine chronic gastritis grading and gastric topographic localization. Keywords: chronic gastritis, cagA gene, Helicobacter pylori, gastric antrum, gastric corpus.
Efek Ekstrak Kulit Manggis terhadap Ekspresi Protein Bcl-2 dan Jumlah Sel Mati Tubulus Ginjal Tikus yang Diinduksi Formalin Marfuati, Nanik; Sarjadi, Sarjadi; Winarto, Winarto; Djamitun, Kis
Jurnal Kedokteran Brawijaya Vol 28, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Kulit manggis mengandung antioksidan yang melindungi terhadap kerusakan sel. Formalin masih disalahgunakan sebagai pengawet makanan yang merupakan sumber oksidan. Penelitian ini dilakukan untuk membuktikan pengaruh ekstrak kulit manggis (EKM) terhadap ekspresi protein Bcl-2 dan jumlah kematian sel tubulus ginjal tikus akibat formalin. Sebanyak 18 tikus Wistar jantan diberi formalin 200mg/kgBB/hari melalui sonde 2 minggu; dibagi 3 kelompok; K tanpa EKM; P1 dan P2 diberi EKM 200 dan 400mg/kgBB/hari seminggu. Dilakukan penilaian ekspresi protein Bcl-2 dan jumlah kematian sel tubulus ginjal tikus. Data dianalisis dengan One Way ANOVA. Analisis ANOVA menunjukkan perbedaan signifikan ekspresi protein Bcl-2 (p=0,006) dan kematian sel tubulus ginjal (p=0,005) antara ketiga kelompok. Ekspresi protein Bcl-2 dan kematian sel tubulus ginjal P2 secara signifikan lebih rendah dibanding K. Berdasarkan penelitian ini dapat disimpulkan EKM 400mg/kgBB/hari selama 1 minggu menurunkan ekspresi protein Bcl-2 dan memperbaiki kerusakan sel tubulus ginjal yang diinduksi formalin.Kata Kunci: Bcl-2, formalin, manggis, kematian sel tubulus ginjal 
Perubahan parameter biologik jaringan kanker payudara mencit akibat pemberian isoflavon tempe Bintari, Siti Harnina; Moeis, Siti Fatimah; Sarjadi, Sarjadi
Jurnal Gizi Klinik Indonesia Vol 9, No 4 (2013): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

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Background: Cancer is severe diseases caused by the changing of deoxyribonucleic acid (DNA) structure. Women who consume soy products have a lower risk for breast cancer, but it cannot be explained the kind of biomolecular aspects of soy product which gives this role.Objective: The aim of this research was to analyze the biological parameter changing of AgNORs, p53, Cas-3 and Bcl-2 in mouse (Mus musculus) C3H strain with breast cancer, as the impact of injecting isoflavone of tempeh.Method: The laboratory experimental research was done by posttest only randomized controlled group design. Subject of study were 18 mice (Mus musculus) C3H strain that were divided into 3 groups, each of which consists of 6 mice. The first group, the control (K), was not injected by isoflavone, while the second group (P1) and the third group (P2) were injected by 1.000 mg and 10.000 mg of tempeh isoflavone/kg diet/day, respectively. All groups were given the transplanted breast cancer cells. AgNORs parameters were selected to determine the level of proliferation and p53 parameters, Cas-3 and Bcl-2 to determine the appearance of apoptosis. Data were analyzed by using Manova and discriminant test for each parameter of cell proliferation.Results: Concentration of isoflavones supplementation with 1,000 and 10,000 mg / kgdiet / day gave effect to the reduction of AgNORs blots, expression of p53 and Cas-3 and increased expression of Bcl-2 in group P1 and P2 compared with control group, as indicated by high statistical values (p=0.000) between both groups or within the group. Results of discriminant test showed that Bcl-2 and AgNORs had dominant role in increased apoptosis and inhibition of cancer cell proliferation in experimental animal.Conclusion: Tempeh isoflavones can be used as a biological parameter change agent that marks the proliferation and apoptosis of mice (Mus musculus) C3H strain towards the normal cell cycle progression.
Efek Moringa oleifera terhadap Gula Darah dan Kolagen Matrik Ekstraseluler Sel β Pankreas Diabetes Eksperimental Ambarwati, Ambarwati; Sarjadi, Sarjadi; Johan, Andrew; Djamiatun, Kis
Jurnal Kedokteran Brawijaya Vol 28, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Daun Moringa oleifera mengandung zat-zat bioaktif yang memperbaiki dan merangsang sel β pankreas untuk mensekresi insulin yang mengatur kadar gula darah. Kelangsungan hidup dan fungsi sel β pankreas memerlukan matriks ekstraseluler (ECM) yaitu fibronektin, laminin dan kolagen. Apakah kadar gula dan kolagen ECM yang mungkin terpengaruh streptozotocin yang merusak sel β pankreas diteliti pada diabetes eksperimental yang diberi perlakukan  M.oleifera. Studi ini menggunakan 24 tikus. Kadar gula darah diukur sebelum dan sesudah induksi streptozotocin 2 hari yang berdampak hiperglikemi, sesudah itu tikus dibagi menjadi 4 kelompok yang terdiri dua grup kontrol yang diterminasi pada hari 0 dan 21 hiperglikemi, dan dua kelompok diperlakukan dengan ekstrak daun M.oleifera dosis 250 dan 500mg/kg BB/hari selama 21 hari. Data dianalisis dengan statistik parametrik dan non-parametrik. Taraf kemaknaan p<0,05. Kadar gula menurun pada masing-masing kelompok perlakukan dosis 250 dan 500mg/BB/kg BB/hari (p=0,000). Kadar gula menjadi normal pada kelompok perlakuan dosis 500mg/kg BB/hari. Ketebalan kolagen ECM pulau Langerhans pankreas tetap normal di semua kelompok penelitian. Ekstrak daun M.oleifera dosis 500 mg/kg BB/hari adalah dosis efektif untuk menormalkan  kadar gula darah pada diabetes eksperimental.Kata Kunci: Diabetes melitus, kadar gula darah, ketebalan kolagen ECM, Moringa oleifera, Streptozotocin,  sel β pankreas
Perubahan parameter biologik jaringan kanker payudara mencit akibat pemberian isoflavon tempe Bintari, Siti Harnina; Moeis, Siti Fatimah; Sarjadi, Sarjadi
Jurnal Gizi Klinik Indonesia Vol 9, No 4 (2013): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

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Abstract

Background: Cancer is severe diseases caused by the changing of deoxyribonucleic acid (DNA) structure. Women who consume soy products have a lower risk for breast cancer, but it cannot be explained the kind of biomolecular aspects of soy product which gives this role.Objective: The aim of this research was to analyze the biological parameter changing of AgNORs, p53, Cas-3 and Bcl-2 in mouse (Mus musculus) C3H strain with breast cancer, as the impact of injecting isoflavone of tempeh.Method: The laboratory experimental research was done by posttest only randomized controlled group design. Subject of study were 18 mice (Mus musculus) C3H strain that were divided into 3 groups, each of which consists of 6 mice. The first group, the control (K), was not injected by isoflavone, while the second group (P1) and the third group (P2) were injected by 1.000 mg and 10.000 mg of tempeh isoflavone/kg diet/day, respectively. All groups were given the transplanted breast cancer cells. AgNORs parameters were selected to determine the level of proliferation and p53 parameters, Cas-3 and Bcl-2 to determine the appearance of apoptosis. Data were analyzed by using Manova and discriminant test for each parameter of cell proliferation.Results: Concentration of isoflavones supplementation with 1,000 and 10,000 mg / kgdiet / day gave effect to the reduction of AgNORs blots, expression of p53 and Cas-3 and increased expression of Bcl-2 in group P1 and P2 compared with control group, as indicated by high statistical values (p=0.000) between both groups or within the group. Results of discriminant test showed that Bcl-2 and AgNORs had dominant role in increased apoptosis and inhibition of cancer cell proliferation in experimental animal.Conclusion: Tempeh isoflavones can be used as a biological parameter change agent that marks the proliferation and apoptosis of mice (Mus musculus) C3H strain towards the normal cell cycle progression.