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IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detecti Sari, Puspita; Wijaya, Christofora Hanny; Sajuthi, Dondin; Supratman, Unang
Jurnal Teknologi Dan Industri Pangan Vol 20, No 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/4312

Abstract

IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detection] Puspita Sari1)*, Christofora Hanny Wijaya2), Dondin Sajuthi3), dan Unang Supratman4) 1) Jurusan Teknologi Hasil Pertanian,Fakultas Teknologi Pertanian, Universitas Jember  Kampus Tegalboto Jl. Kalimantan I, Jember 68121 2) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor Kampus IPB Darmaga, Bogor 16680 3) Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680  4) Jurusan Kimia, FMIPA, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Jatinangor, Sumedang 45363   Diterima  1 Juli  2009 / Disetujui  7 Desember 2009   ABSTRACT   The aim of this study was to determine the content of total monomeric anthocyanins and to identify the anthocyanin composition of jambolan fruit (Syzygium cumini) growing in Indonesia. Anthocyanins were extracted with methanol containing 0.1% HCl, removed from non-polar impurities by partition with chloroform and purified in a C18 solid phase cartridge. Anthocyanins were identified by high-performance liquid chromatography with diode array detection (HPLC-DAD), UV-visible spectral analysis, and physico-chemical reactions. Using pH-differential method, it was known that the content of total monomeric anthocyanin in the ripe fruit was 161 mg/100 g fresh fruit (731 mg/100 g skin on wet basis or 3430 mg/100 g skin on dry basis), based on cyanidin-3-glucoside. Furthermore, five anthocyanins without acylating groups were identified as 3,5-diglucoside derivatives of delphinidin (41.29%), petunidin (27.79%), malvidin (25.60%), cyanidin (4.19%), and peonidin (1.13%). These results demonstrate that the skin of jambolan fruit especially the ripe one is rich in anthocyanin, with wide range in anthocyanidin backbone (five aglycons).   Key words: jambolan fruit, Syzygium cumini, anthocyanin, HPLC-DAD
IMPROVING STUDENTS’ SPEAKING ACHIEVEMENT THROUGH PUBLIC SPEAKING TASKS Sari, Puspita
TRANSFORM Journal of English Language Teaching and Learning of FBS UNIMED Vol 1
Publisher : Universitas Negeri Medan

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Abstract

This study deals with improving students’ speaking achievement through public speaking tasks. This study was conducted by using classroom action research. The subject of the research was class XI AP2 SMK BM Taman Siswa Lubuk Pakam consisted of 32 students.  The research was conducted in two cycles and every cycle consisted of four meetings. The instruments for collecting data were oral test for quantitative data and diary notes and interview for qualitative data. Based on the oral test scores, students’ score kept improving in every test. In the orientation test, the mean score was 55.9, in the first competence test, the mean score was 64.4 and in the second competence test, the mean score was 73. The improvement also can be seen from the percentage of the students’ speaking achievement, in the orientation test only 3.13% (one student) got 65 points. In the first competence test 65.63% (twenty one students) got 65 %. It means there was an improvement about 62.5%. In the second competence test 93.75% (thirty students) got 65 points. The improvement was 31.25%. It can be concluded that public speaking tasks could improve students’ speaking achievement.   Key Words: Speaking, Achievement, Public Speaking, Tasks  
HUBUNGAN KEPADATAN JENTIK Aedes sp DAN PRAKTIK PSN DENGAN KEJADIAN DBD DI SEKOLAH TINGKAT DASAR DI KOTA SEMARANG Sari, Puspita
xxxx Vol 1, No 2 (2012): JURNAL KESEHATAN MASYARAKAT
Publisher : Jurnal Kesehatan Masyarakat

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Abstract

Demam berdarah dengue (DBD) adalah penyakit menular yang disebabkan oleh virus Dengue dan dapat mengakibatkan kematian. Penyakit ini sering menyerang anak-anak, terutama anak sekolah dasar sehingga diduga sekolah dapat menjadi tempat yang potensial dalam penyebaran penyakit DBD. Tujuan dari penelitian ini adalah untuk mengetahui hubungan kepadatan jentik Aedes sp dan praktik PSN terhadap kejadian DBD pada siswa sekolah dasar di Kota Semarang. Penelitian ini merupakan penelitian penjelasan dengan metode cross sectional. Populasi dalam penelitian ini adalah seluruh SD di Kota Semarang dengan jumlah total 602 SD. Sampel penelitian sebanyak 75 SD yang diambil menggunakan metode proportional multistage random sampling. Analisis data menggunakan uji Chi Square dengan taraf signifikansi 95%. Hasil penelitian menunjukan 38,7% sekolah terdapat penderita DBD dalam satu tahun terakhir, 78,7% sekolah memiliki kepadatan jentik tinggi, dan 85,3% sekolah tidak melakukan PSN dengan baik. Nilai kepadatan jentik Sekolah Dasar di Kota Semarang adalah CI=23,4%, HI=78,7%, BI=236, dan DF=8. Sekolah yang melakukan praktik menguras TPA minimal seminggu sekali sebesar 86,7%, sebagian besar sekolah (66,7%) melakukan praktik menyikat TPA, 82,7% tidak melakukan praktik menutup TPA, dan 53,3% tidak melakukan praktik mengubur barang bekas atau membuangnya di tempat sampah yang tertutup. Dari hasil uji statistik didapatkan bahwa ada hubungan yang bermakna antara kepadatan jentik Aedes sp (p=0,0001) dan praktik PSN (p=0,005) dengan kejadian DBD di Sekolah Dasar di Kota Semarang. Berdasarkan hasil penelitian, disarankan untuk meningkatkan pengetahuan dan pemahaman serta kesadaran seluruh warga sekolah mengenai Pemberantasan Sarang Nyamuk (PSN) DBD.
IMPROVING STUDENTS’ SPEAKING ACHIEVEMENT THROUGH PUBLIC SPEAKING TASKS Sari, Puspita
TRANSFORM Journal of English Language Teaching and Learning of FBS UNIMED Vol 1, No 1 (2012)
Publisher : Universitas Negeri Medan

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Abstract

This study deals with improving students’ speaking achievement through public speaking tasks. This study was conducted by using classroom action research. The subject of the research was class XI AP2 SMK BM Taman Siswa Lubuk Pakam consisted of 32 students.  The research was conducted in two cycles and every cycle consisted of four meetings. The instruments for collecting data were oral test for quantitative data and diary notes and interview for qualitative data. Based on the oral test scores, students’ score kept improving in every test. In the orientation test, the mean score was 55.9, in the first competence test, the mean score was 64.4 and in the second competence test, the mean score was 73. The improvement also can be seen from the percentage of the students’ speaking achievement, in the orientation test only 3.13% (one student) got 65 points. In the first competence test 65.63% (twenty one students) got 65 %. It means there was an improvement about 62.5%. In the second competence test 93.75% (thirty students) got 65 points. The improvement was 31.25%. It can be concluded that public speaking tasks could improve students’ speaking achievement.   Key Words: Speaking, Achievement, Public Speaking, Tasks  
Hubungan Antara Kecemasan dengan Keluhan Nyeri Ulu Hati pada Pasien Rawat Jalan di Poli Penyakit Dalam RSD. dr. Soebandi Sari, Puspita; Mardijana, Alif; Purwandhono, Azham
Pustaka Kesehatan Vol 2, No 1 (2014)
Publisher : Pustaka Kesehatan

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Anxiety is a tension, insecurity and fears that arise because of feeling that something bad will happen, it is one of psychiatric disorder causing a somatization disorder. A common somatization disorder is an epigastric pain. Epigastric pain is subjective physical complaint located at the epigastric region. This study aimed to determine whether there was a correlation between anxiety and epigastric pain symptom on outpatients of Internal Medicine dr. Soebandi hospital. This was an observational analytic study with cross sectional approach. Measurement of anxiety level using questionnaires HARS ( Hamilton Anxiety Rating Scale ) and pain level measurements using questionnaires VAS ( Visual Analog Scale ). Data was obtained using questionnaires by interview technique. The result showed a significant relationship ( p = 0.000 ) between the anxiety and epigastric pain symptom. Analysis of the relationship between the two variables showed a positive correlation 0.688. In conclusion, there was a positive correlation between anxiety-disorders and epigastric pain symptom. Keywords: anxiety, epigastric pain, HARS, VAS
FORMULASI TABLET EFFERVESCENT BERBAHAN BAKU KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) DAN BUAH SALAM (Syzygium polyanthum [Wight.] Walp) Pribadi, Yoga Sindi; Sukatiningsih, .; Sari, Puspita
Berkala Ilmiah Pertanian Vol 1, No 4: MEI
Publisher : Berkala Ilmiah Pertanian

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[ENGLISH] Red dragon fruit peel, which is 30-35% from red dragon fruit, is supposed to be a waste containing betacyanin ± 186,90 mg/100 g and antioxidant activity ± 53,71 %. Another potential fruit, which can be developed as an antioxidant, is a salam fruit. Salam fruit is one of local fruits in Indonesia, which has a high antioxidant but its potential has not been utilized optimally. The aim of this research is to determine the exact formulation in effervescent tablet from red dragon fruit peel and salam fruit’s extracts through sensory, physical as well as chemical characteristics. This research was conducted in three steps. Firstly, the researchers made the preparation of raw materials to get the red dragon fruit peel and flesh of salam fruit. Secondly, extraction using 97% ethanol and vacuum drying to obtained extract powder of red dragon fruit peel and salam fruit. At last, determines the formulation of effervescent tablet. Effervescent tablets were analyzed sensory, physical and chemical characteristics. Sensory analysis showed F1, F2 and F3 having the preferred formulation. Furthermore, the results of physical and chemical characteristics analysis indicate F3 having the best formulation with the highest antioxidant activity 63,13% with speed-soluble 72.40 seconds, water content of 11.24%, color parameters L = 41,32, C = 23 and hue = 358,74 (red purple), betacyianin content 309,75 mg/100 g dry basis and anthocyianin content 5,26 mg/100 g dry basis. Keywords: red dragon fruit peel; salam fruit; effervescent tablet [INDONESIAN] Kulit buah naga merah merupakan limbah (30 - 35% buah utuh) mengandung betasianin ± 186,90 mg/100 g dan aktivitas antioksadan ± 53,71%. Buah lainnya yang potensial dikembangkan sebagai sumber antioksidan adalah buah salam. Buah salam adalah salah satu buah-buahan lokal Indonesia yang mengandung antioksidan, tetapi belum dimanfaatkan secara optimal. Tujuan dari penelitian ini adalah untuk mengetahui formulasi yang tepat tablet effervescent yang dibuat dari ekstrak kulit buah naga merah dan buah salam melalui pengujian karakteristik sensori, fisik, dan kimia. Penelitian dilakukan dalam tiga tahapan. Pada tahap pertama, peneliti melakukan persiapan bahan baku untuk mendapatkan kulit buah naga merah dan buah salam tanpa biji. Tahap kedua melakukan ekstraksi menggunakan pelarut etanol (97%) dan pengeringan ekstrak menggunakan pengering vakum untuk mendapat bubuk ekstrak kulit buah naga merah dan buah salam. Tahap terakhir melakukan pengujian tablet effervescent. Tablet effervescent dianalisa karakteristik sensori, fisik, dan kimia. Analisis sensori menunjukkan bahwa formulasi F1, F2, dan F3 lebih disukai panelis. Lebih lanjut, hasil dari analisis fisik dan kimia menunjukkan bahwa formulasi F3 merupakan formulasi terbaik dengan nilai aktivitas antioksidan tertinggi 63,13%, waktu larut 72,40 detik, kadar air 11,24%, parameter warna L = 41,32; C =23; dan hue = 358,74 (red purple), kandungan betasianin 309,75 mg/100 g (berat kering), dan kandungan antosianin 5,26 mg/100 g (berat kering). Kata kunci: kulit buah naga merah; buah salam; tablet effervescent How to citate: Pribadi YS, Sukatiningsih, P Sari. 2014. Formulasi tablet effervescent berbahan baku kulit buah naga merah (Hylocereus polyrhizus) dan buah salam (Syzygium polyanthum [Wight.] Walp). Berkala Ilmiah Pertanian 1(4): 86-89.
HUBUNGAN AKTIVITAS ENZIM DAN KONSENTRASI SUBSTRAT PADA POLA DETEKSI SECARA HPLC HASIL TRANSGLIKOSILASI NARINGENIN OLEH ENZIM SELULASE Penicillium sp. LBKURCC27 Sari, Puspita; Nugroho, Titania Tjandrawati; Dahliaty, Andi
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Transglycosylation of naringenin was carried out by concentrated cellulase enzyme of Penicillium sp. LBKURCC27. Enzymatic transglycosylation of naringenin was successfully carried out by cellulase from Penicillium sp. LBKURCC27, however the reproducibility of the reaction is low, making detection by reverse phase HPLC (High Performance Liquid Chromatography) difficult when the enzyme activity decreases. It is believe that the flavonoid concentration used in the transglycosylation naringenin by cellulase Penicillium sp. LBKURCC27 influences the detection pattern of transglycosylation product of HPLC. The reaction was carried out for 30 hours at 40°C with acetate buffer 0.05 M pH 5.5 and 170 rpm shaking. Carboxymethyl Cellulose (CMC) was used as glycosyl donor. Results of HPLC analysis showed that cellulase Penicillium sp. LBKURCC27 with an activity of (0.670±0.023 U/mL) were not able to convert naringenin to a detectable glycosylated product when the initial substrate concentration was 6 mg/mL. In the 0.6 mg/mL of naringenin concentration, the glycosylation product was formed with 100% of percent convertion.
IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detecti Sari, Puspita; Wijaya, Christofora Hanny; Sajuthi, Dondin; Supratman, Unang
Jurnal Teknologi dan Industri Pangan Vol 20, No 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to determine the content of total monomeric anthocyanins and to identify the anthocyanin composition of jambolan fruit (Syzygium cumini) growing in Indonesia. Anthocyanins were extracted with methanol containing 0.1% HCl, removed from non-polar impurities by partition with chloroform and purified in a C18 solid phase cartridge. Anthocyanins were identified by high-performance liquid chromatography with diode array detection (HPLC-DAD), UV-visible spectral analysis, and physico-chemical reactions. Using pH-differential method, it was known that the content of total monomeric anthocyanin in the ripe fruit was 161 mg/100 g fresh fruit (731 mg/100 g skin on wet basis or 3430 mg/100 g skin on dry basis), based on cyanidin-3-glucoside. Furthermore, five anthocyanins without acylating groups were identified as 3,5-diglucoside derivatives of delphinidin (41.29%), petunidin (27.79%), malvidin (25.60%), cyanidin (4.19%), and peonidin (1.13%). These results demonstrate that the skin of jambolan fruit especially the ripe one is rich in anthocyanin, with wide range in anthocyanidin backbone (five aglycons). 
PENGARUH KOMUNIKASI DAN KEPUASAN TERHADAP KEPERCAYAAN DAN KOMITMEN UNTUK MELAKUKAN HUBUNGAN PEMASARAN JANGKA PANJANG MULYANA, MULYANA; SARI, PUSPITA
Jurnal Ekonomi & Bisnis 2009: EKOBIS (Vol.10 No.2 2009)
Publisher : Department of Management, Faculty of Economics, Universitas Islam Sutan Agung, Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.30659/ekobis.10.2.344-353

Abstract

This research is study the process which influence the relationship development (marketing)based on commitment. The excellence of continual competitive from company can do with thelong-range cooperation relationship. In this cooperation relationship, the role of commitmentis very importance. Research in Juwana indicate that trust is the primary factor in courseof development of long-range marketing cooperation commitment. Trust is influenced bysatisfaction and communications. Type of research is explanatory research, population inthis research is all of cutomer. Technique used in this research is census so all of populationwill be sampel. Technic analysis used in this research is equation model of structural byPartial Least Square ( PLS). Communications and satisfaction have an influence to trust thensatisfaction and communications also have an influence on directly to commitment. Trust inthis case also have an influence to commitment.Keywords : Communication, Satisfaction, Trust, Commitment
RELATIONSHIP BETWEEN THE POPULATION DENSITY AND THE OCCURRENCE OF DENGUE HEMORRHAGIC FEVER IN PALU AT 2010-2014 A.R, Rahmi; Sari, Puspita
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan Vol 4, No 1 (2017)
Publisher : Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran dan Ilmu Kesehatan

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 Background:  Dengue hemorrhagic fever (DHF) represents one of public health problems in Indonesia and so do the other  countries. Commonly, DHF infections are found in tropics and subtropics  country, specially in urban and sub urban area. The number of sufferer and its distribution increases along with mobility and population density.  Methods: The study used  analytic observational method with cross sectional design. The samples  were number of  DHF cases in Palu City at 2010-2014 that recorded in Health Department of Palu. The data used in form secondary data of DHF cases through Health Department of Palu’s records and population density through Statistic of Palu City. Data processing was done with SPSS program with spearman test. Results: The results showed that r value = 0,502 and p value = 0,000 which means that there is a moderate relationship with positive direction between the population density and the occurrence of dengue hemorrhagic fever  Conclusions: There is a moderate relationship with positive direction between the population density and the occurrence of dengue hemorrhagic fever in Palu City at 2010-2014.   Key words: occurrence of DHF, population density