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Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract Sandi, Sofia; Miksusanti, Miksusanti; Sari, Meisji Liana; Sahara, Eli; Supriyadi, Adi; Gofar, Nuni; Asmak, Asmak
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.
The growth allometrically Mandalung and histological muscle fiber leg Sari, Meisji Liana
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 4 (2003): DECEMBER 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

This study was conducted to compare the growth alometrically performance especially rate of muscle growth microscopically of Mandalung offspring of Muscovy x Duck (MD) and Duck x Muscovy (DM). Fifty-one Mandalung duck of IE and EI were used. They were fed with 2-type broiler commercial feed, which were starter type (day 1 – week 4) and finisher type (week 4 – week 12). Starter feed contained of 20% protein and 2800 kcal/kg energy while finisher feed contained of 18% protein and 3000 kcal/kg energy. Data on weights of slaughtered body and body parts (head, neck, fore back, rear back, breast, upper leg, lower leg, foot and its membrane) were measured at week 1, 4, 8 and 12. Diameter of muscle fiber was measured at age 8, 10 and 12 weeks, and each slaughter was represented by 1, 2 and 3 ducks. The results showed The growth direction was similar in IE and EI, initiated from head, to the neck, fore back and rear back, and from foot, to the thigh, breast and wings. Diameter of muscle fibers of Mandalung duck offspring of EI were larger (14,49 vs 13,37; 20,02 vs. 15, 24 μm; 23,68 vs. 18, 08 μm) than the IE.  Key words: Allometry, histological, muscle fiber
The growth allometrically Mandalung and histological muscle fiber leg Sari, Meisji Liana
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 4 (2003)
Publisher : Indonesian Animal Sciences Society

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Abstract

This study was conducted to compare the growth alometrically performance especially rate of muscle growth microscopically of Mandalung offspring of Muscovy x Duck (MD) and Duck x Muscovy (DM). Fifty-one Mandalung duck of IE and EI were used. They were fed with 2-type broiler commercial feed, which were starter type (day 1 – week 4) and finisher type (week 4 – week 12). Starter feed contained of 20% protein and 2800 kcal/kg energy while finisher feed contained of 18% protein and 3000 kcal/kg energy. Data on weights of slaughtered body and body parts (head, neck, fore back, rear back, breast, upper leg, lower leg, foot and its membrane) were measured at week 1, 4, 8 and 12. Diameter of muscle fiber was measured at age 8, 10 and 12 weeks, and each slaughter was represented by 1, 2 and 3 ducks. The results showed The growth direction was similar in IE and EI, initiated from head, to the neck, fore back and rear back, and from foot, to the thigh, breast and wings. Diameter of muscle fibers of Mandalung duck offspring of EI were larger (14,49 vs 13,37; 20,02 vs. 15, 24 μm; 23,68 vs. 18, 08 μm) than the IE.  Key words: Allometry, histological, muscle fiber
The growth allometrically Mandalung and histological muscle fiber leg Sari, Meisji Liana
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 4 (2003): DECEMBER 2003
Publisher : Indonesian Animal Sciences Society

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Abstract

This study was conducted to compare the growth alometrically performance especially rate of muscle growth microscopically of Mandalung offspring of Muscovy x Duck (MD) and Duck x Muscovy (DM). Fifty-one Mandalung duck of IE and EI were used. They were fed with 2-type broiler commercial feed, which were starter type (day 1 – week 4) and finisher type (week 4 – week 12). Starter feed contained of 20% protein and 2800 kcal/kg energy while finisher feed contained of 18% protein and 3000 kcal/kg energy. Data on weights of slaughtered body and body parts (head, neck, fore back, rear back, breast, upper leg, lower leg, foot and its membrane) were measured at week 1, 4, 8 and 12. Diameter of muscle fiber was measured at age 8, 10 and 12 weeks, and each slaughter was represented by 1, 2 and 3 ducks. The results showed The growth direction was similar in IE and EI, initiated from head, to the neck, fore back and rear back, and from foot, to the thigh, breast and wings. Diameter of muscle fibers of Mandalung duck offspring of EI were larger (14,49 vs 13,37; 20,02 vs. 15, 24 μm; 23,68 vs. 18, 08 μm) than the IE.  Key words: Allometry, histological, muscle fiber
MELALUI PELATIHAN PEMBUATAN TELUR ASIN RENDAH SODIUM UNTUK MENIGKATKAN PENDAPATAN PETERNAK ITIK PEGAGAN DI DESA 1 KOTA DARO II KECAMATAN RANTAU PANJANG KABUPATEN OGAN ILIR Sari, Meisji Liana
Jurnal Pengabdian Sriwijaya Vol 3, No 2 (2015)
Publisher : Jurnal Pengabdian Sriwijaya

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Abstract

Desa Kota Daro II  merupakan daerah yang potensi untuk budidaya ternak itik,  hal ini memungkinan produk yang dihasilkan dari budidaya tersebut yaitu berupa telur juga bisa meningkat. Telur adalah salah satu sumber protein hewani yang memilik rasa yang lezat,  mudah dicerna, dan bergizi tinggi. Selain itu telur mudah diperoleh dan harganya murah. Tujuan dari kegiatan pengbdian ini  untuk meningkatkan gizi dan ekonomi masyarakat melalui peningkatan minat konsumsi telur asin rendah sodium. Dalam kegiatan ini metode yang digunakan  adalah sosialiasasi dan penyuluhan, pelatihan  dan demontrasi pembuatan telur asin  dan pembinaan. Penyuluhan ini cukup berhasil,  walaupun  dari jumlah beserta hanya 15 orang akan tetapi jumlah pertanyaan yang muncul selama diskusi cukup banyak.  Pada peserta sangat antusius  dengan penyuluhan  yang dilakukan oleh tim pengabdian. Kesimpulan dari kegiatan ini adalah telur asin sangat potensial dikembangkan untuk meningkatkan kebutuhan gizi dan ekonomi masyarakat Desa Kota Daro II.
Polimorfisme Protein Darah Itik Pegagan dengan Metode PAGE Sari, Meisji Liana; Noor, Ronny Rachman; Hardjosworo, Peni S.; Nisa, Chairun
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

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Abstract

polymorphism of blood protein pegagan duck by PAGE methodABSTRACT. Pegagan duck is one of local duck species which comes from Ogan Ilir (OI) Regency, South Sumatera. This species is not popular, yet. But, it has spread out along Ogan river which comprises of three districts e.g Tanjung Raja, Inderalaya and Pemulutan. The ecosystem in this three districts is dominated by swampy area. As far, there is relatively limited information of Pegagan duck compared to another local duck. So, for the first step was done a reseach to identify genetic characteristic from blood protein analyses by electrophoresis method. It is expected that this information will become the bases in determining of the policy of Pegagan duck development. Polymorphism analyses of blood protein was done with 9 duck samples which taken randomly from 400 pegagan ducks. Protein locus observed were albumin (Alb), post albumin (Pa), transferrine (Tf), post transferrine-1 (Ptf-1), and post transferrine-2 (Ptf-2). Polymorphism analyses of blood protein of pegagan duck was characterized by allele A in locus Transferrine (TfA), Post transferrine-1 (Ptf-1A) and Post transferrine-2 (Ptf-2A) . Genetic diversity based on blood protein of Pegagan duck showed that gen frequency value in a range of 0,11 – 0,94 and high heterozygosis value that was 0,62 %.
Pengaruh Pemberian Grit Kerang dan Cahaya terhadap Kualitas Kerabang Telur Ayam Arab (Silver Brakel kriel) Sari, Meisji Liana
Jurnal Peternakan Sriwijaya Vol 1, No 1 (2012): Jurnal Peternakan Sriwijaya
Publisher : Program Studi Peternakan Fakultas Pertanian Universitas Sriwijaya

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Abstract

Tujuan penelitian adalah untuk mempelajari pengaruh pemberian kerang grit dan cahaya terhadap kualitas kerabang telur Ayam Arab. Penelitian ini menggunakan rangcangan acak lengkap faktorial 2x2 dengan 3 kali ulangan dan masing-masing ulangan ditempatkan ayam sebanyak 5 ekor. Parameter yang diamati antara lain tebal kerabang, berat kerabang, dan indeks kerabang telur. Data dianalisis dengan analisis sidik ragam dan jika hasilnya berbeda nyata dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa perlakuan pemberian grit kerang dan cahaya tidak menunjukkan hasil yang signifikan terhadap tebal dan berat kerabang, akan tetapi signifikan terhadap indeks kerabang telur.Kata kunci : Ayam arab, grit kerang, kerabang telur
CONCENTRATIONS TEST OF METHANE GAS FROM SWAMP FORAGE SILAGE WITH IN VITRO METHOD Sandi, Sofia; Gofar, Nuni; Sari, Meisji Liana; Yosi, Fitra; Untari, Budi; Kartika, Erra
BIOVALENTIA: Biological Research Journal Vol 3, No 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

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Abstract

Aims of this study to concentration test of methane gas from swamp forage silage with in vitro methods.This study used Completely Randomized Design with 3 treatments and each treatment consisted of 5 replications. The treatments used are as follows: P1 (100% kumpai tembaga grass (Hymenachne acutigluma)), P2 (50% kumpai tembaga grass (Hymenachne acutigluma) + 50% kemon air (Neptunia oleracea lour)), P3 (100% kemon air ( Neptunia oleracea lour)). The parameters observed N-Ammonia, total gas production, methane gas concentrations in vitro and VFA partially. The results of variance showed that treatment significantly different (P <0.05) with methane concentration  in vitro with highest gas concentration treatment of silage made from 50%  kumpai tembaga grass (Hymenachne acutigluma) and  50% kemon air (Neptunia oleracea lour).Keywords: Concentration, Methane, Silage Forage Swamp, In Vitro.
Isolasi dan Karakterisasi Bakteri Asam Laktat pada Usus Ayam Broiler Sari, Meisji Liana; Abrar, Arfan; Merint, Merint
Jurnal Agripet Vol 13, No 1 (2013): Volume 13, No. 1, April 2013
Publisher : Agricultural Faculty

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Abstract

The isolated and characteristic lactic acid bacteria in broiler’s intestineABSTRACT. The objective of the research was to evaluate and characterize of a Lactic Acid Bacteria isolated frombroiler’s small intestine. The research was done by three steps : (1) Isolated Lactic Acid Bacteria, (2) Gram colourize and microscopic observation and (3) Inhibitory test with pathogen bacteria. The isolation of lactic acid bacteria was proceed by clone from broiler’s small intestine with MRS Broth for 24 hours, thinned until 10 ̄ 9with serial number, invested in MRS jell, purify by streak plate method and storage the lactic acid bacteria in an MRS angle jell. Gram colourize was done with crystal violet liquid, Iodium, Alkohol 95% and  Safranin, and also microscopic observation was done by electron microscope with 40 zooming. Inhibitory test with pathogen bacteria was evaluated with  Escherichia coli as testing bacteria in 2, 4, 6, 8, 10, 12, 24 dan 48 hours incubation period. The isolation result showed two sample of isolate (A1 dan A2) and consist of the observation in shape as well as colour of the bacteria growth, for sample of isolate A1 the colonies had coccus  and white colour. The isolate of A2 had sarcinar. After that, gram colouring was done and microscopic observation were identified into gram-negative bacteria became bacill and bacill cocco. Inhibitory test with pathogen bacteria showed that it produced antimicrobial with 11 mm clearing zone diameter. The result of the research got Lactobacillus sp as the genera of Lactic Acid Bacteria. 
PEMANFAATAN TANAMAN INDIGOFERA SP DAN ABU PELEPAH SAWIT PADA PEMBUATAN TELUR ASIN FUNGSIONAL ITIK PEGAGAN DI DESA SEJARO SAKTI KECAMATAN INDERALAYA KABUPATEN OGAN ILIR Sari, Meisji Liana
Jurnal Pengabdian Sriwijaya Vol 6, No 1 (2018)
Publisher : Jurnal Pengabdian Sriwijaya

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Abstract

Desa Sejaro Sakti merupakan daerah yang potensi untuk budidaya ternak itik,  hal ini memungkinan produk yang dihasilkan dari budidaya tersebut yaitu berupa telur juga bisa meningkat. Telur adalah salah satu sumber protein hewani yang memilik rasa yang lezat,  mudah dicerna, dan bergizi tinggi. Selain itu telur mudah diperoleh dan harganya murah. Tujuan dari kegiatan pengbdian ini  untuk meningkatkan gizi dan ekonomi masyarakat melalui peningkatan minat konsumsi telur asin rendah sodium. Dalam kegiatan ini metode yang digunakan  adalah sosialiasasi dan penyuluhan, pelatihan  dan demontrasi pembuatan telur asin  dan pembinaan. Penyuluhan ini cukup berhasil,  walaupun  dari jumlah beserta hanya 15 orang akan tetapi jumlah pertanyaan yang muncul selama diskusi cukup banyak.  Pada peserta sangat antusius  dengan penyuluhan  yang dilakukan oleh tim pengabdian. Kesimpulan dari kegiatan ini adalah telur asin sangat potensial dikembangkan untuk meningkatkan kebutuhan gizi dan ekonomi masyarakat Desa Sejaro Sakti