Faralinda Sari
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The Comparison of One and Two Steps Equilibration in Vitrification Process on The Morphology and Viability of Mouse Blastocysts Djuwita, Ita; Sari, Faralinda; Mohamad, Kusdiantoro
Jurnal Veteriner Vol 11, No 3 (2010)
Publisher : Jurnal Veteriner

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Abstract

A study was conducted to compare the effect of one and two steps equilibration method of vitrificationon the morphology and viability of mouse blastocysts. Blastocysts were firstly exposed to modified PhosphateBuffered saline (mPBS) containing 1% Bovine Serum Albumin (BSA) proceeded by exposure in mPBSrespectively containing 0.25M sucrose (S) for 2 minutes . Blastocysts were then exposed for 2 minutesrespectively to mPS+0.5M S (one step method) or in mPBS+0.5M S+10% ethylene glycol (EG) (two stepmethod).. Blastocysts were then exposed in mPBS+0.5M S+30% EG for 60 second, loaded into 0.25 mlplastic straw, and exposed immediately in vapor of liquid nitrogen for 10 second before they were and thenplunged into liquid nitrogen. The blastocysts were reconstituted by diluting with mPBS+0.5M S followedby mPBS+0.25M S for each 3 min and washed in mPBS without sucrose. The viability of cells was assessedby fluorescent vital staining, by re-expansion for 24 hours in vitro culture, and by implantation into therecipient oviduct. The percentages of morphologically normal blastocysts following recovery fromvitrification were higher (p<0.05) in one step equilibration than in those of two steps methods (89.6%. vs82.6%). The viability of blastocysts examined under light microscope after staining with biz-benzimidizepropidiumiodine and 24 hours in vitro culture in one step methods (64.0%; 57.8%) were higher (p<0.05)compared with two steps methods (40.0%; 35.6%), respectively. The implantation rate of vitrifiedblastocysts (23.1%) was not significantly different to that of fresh blastocysts (33.4%). These resultsshowed that the one and two step equilibration methods are effective for vitrification and maintaining theviability of the mouse blastocysts.
Identifikasi Spesies Babi pada Produk Pangan Asal Hewan di Pasar Tradisional Povinsi Riau dengan Metode Polymerase Chain Reaction SARI, FARALINDA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Indonesia adalah negeri dengan jumlah muslim terbanyak didunia. Kehalalan produk pangan menjadi suatu prioritas utama. Pemerintah harus menjamin keamanan pangan dan kehalalan produk, sehinga konsumen dapat memperoleh pangan yang aman, sehat, utuh dan halal. namun peraturan pengusaha untuk mendapatkan labelisasi yang legal dari LPOM-MUI masih sebatas himbauan, belum menjadi kewajiban bagi semua pedagang pangan. Pemantauan labelisasi halal hanya terbatas kepada produk pangan dalam kemasan, dan belum menyentuh produk pangan yang dipasarkan secara tradisional. Tulisan ini merupakan hasil pengujian identifikasi spesies babi yang dilakukan kepada 204 sampel produk pangan asal hewan, dengan menggunakan metode PCR konvensional. Kegiatan ini dilakukan pada bulan Februari sampai dengan Desember 2016 di 12 Kabupaten/Kota se Provinsi Riau, dengan sasaran penjual makanan tradisional, non kemasan. Hasil pengujian ini menemukan terdapat 2 sampel positif mengandung DNA babi dari total 204 sampel yang diuji.