Evi Maya Sari
Program Studi Teknologi Hasil Perikanan, Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

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KAJIAN SENYAWA BIOAKTIF EKSTRAK TERIPANG HITAM (Holothuria edulis) BASAH DAN KERING SEBAGAI ANTIBAKTERI ALAMI

Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Volume 3, Nomor 4, Tahun 2014
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

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Abstract

Holothuria edulis merupakan salah satu sumber hayati laut yang mempunyai banyak manfaat dan memiliki senyawa bioaktif. Tujuan dilakukannya penelitian ini untuk mengetahui kandungan senyawa bioaktif dan daya hambat terbaik ekstrak H. edulis basah dan kering serta  pengaruh perbedaan konsentrasi pelarut dan keadaan sempel sebagai aktivitas antibakteri Bacillus cereus dan Pseudomonas aeruginosa. Hasil dari penelitian didapatkan bahwa ekstrak etil asetat H. edulis dalam keadaan basah dengan konsentrasi 450mg/ml merupakan ekstrak terbaik. Hasil dari ekstrak H. edulis basah menunjukkan aktivitas antibakteri tertingi dengan daya hambat bakteri terluas pada bakteri B. cereus 2.8±0.2 mm dan P. aeruginosa yaitu 8.6±0.2 mm, sedangkan pada H. edulis kering pada B. cereus 1.7±0.1 mm dan P. aeruginosa yaitu 5.5 ±0.2 mm. Berdasarkan uji fitokimia dari ekstrak H. edulis basah dan kering positif mengandung alkaloid, triterpenoid, steroid, flavonoid, dan saponin. Holothuria edulis is one of marine resources that has many benefit and have bioactive compounds. The purpose of this research was to determine the content of bioactive compounds and the best extract inhibition of wet and dry H. edulis as well as the influence of the different solvent concentration and sample condition as the antibacterial activity of Bacillus cereus and Pseudomonas aeruginosa. Results of the research found that the best extracts of H. edulis is ethyl acetate extract of wet H. edulis in 450 m g/ml concentration. Results extracts of wet H. edulis show that the highest antibacterial activity with the wide blocked power on B. cereus is 2.8 ± 0.2 mm and P. aeruginosa is 8.6 ± 0.2 mm, meanwhile in dry H. edulis on B. cereus is 1.7 ± 0.1 mm and P. aeruginosa is 5.5 ± 0.2 mm. Based on phytochemicals test of wet and dry H. edulis extracts positive contains alkaloid, triterpenoid, steroid, flavonoid and saponin.

AMINO ACID PROFILE AND BIOACTIVE COMPOUNDS OF SEAHORSE Hippocampus comes

Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 9, No 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Bogor Agricultural University

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Abstract

Seahorse is one of the marine living  resources usually used as ornamental fish, traditional medicinal materials, and souvenirs. The purpose of the study was to determine the proximate composition of wet and dry seahorses, determine the profile of amino acid hydrolyzate and powder of seahorses, and determines to content of bioactive compounds from the ethanol extract of seahorses on qualitatively. The sample of this study is seahorses obtained from nature. Prviously, seahorses were morphometric identified, subsequently, seahorses were made of the powder, hydrolyzate, and ethanol extract. Several analyzes used were qualitative analysis of proximate, amino acid, and phytochemical analysis. Morphometric identification results indicate that the type is Hippocampus comes. The proximate composition is water content is 66.16 ± 0.33% (wet) and 10.33 ± 0.16% (dry), ash content of 9.55 ± 0.15% (wet) and 9.65 ± 0.16% (dry), lipid content 1.18 ± 0.23% (wet) and 4.89 ± 0.37% (dry), protein content of 22.73 ± 0.17% (wet) and 69.83 ± 0.31% (dry), carbohydrate 0.39 ± 0.23 (wet) and 5.50 ± 0.34 (dry). The amino acid composition both on hydrolyzate and powder comprising 9 essential amino acids are lysine, leucine, isoleucine, phenylalaline, valine, methionine, histidine, arginine, and threonine and 6 non essential amino acids are tyrosine, alanine, glycine, serine, glutamic acid, and aspartic acid. The results of identification of bioactive compounds is flavonoids, triterpenoids, steroids, saponins, and phenol of hydroquinone. Keywords: Hippocampus comes, proximate analysis, amino acid, and bioactive compounds