Desi Sandra Sari
Bagian Periodonsia, Fakultas Kedokteran Gigi Universitas Jember

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AKTIVITAS ANTIBAKTERI DAN KLT BIOAUTOGRAFI EKSTRAK ETANOL DAUN SISIK NAGA (Drymoglossum piloselloides) TERHADAP Streptococcus mutans Ulfa, Evi Umayah; Sari, Desi Sandra; Wijaya, Dhani
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 10, No 1 (2013)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Abstract

Sisik naga (Drymoglossum piloselloides) has been used empirically for dysentry and candidiasis. This diseases were caused by bacteria and fungi. The objective of this research was to evaluate the antibacterial effect and to find out the antimicrobial compound of sisik naga. Leaf of sisik naga was extracted with ethanol 90% by using maseration. This extract were tested for antibacterial activity against Streptococcus mutans using agar difussion. TLC Bioautography methods was used to identify the active compound. Result of antibacterial test showedf ethanolic extract of sisik naga had antibacterial effect against S. mutans. Bioautography showed one spot on Rf =0 give clear zone.
KADAR CALPROTECTIN CAIRAN SULKUS GINGIVA PADA KEDALAMAN POKET PERIODONTAL YANG BERBEDA PENDERITA AGGRESSIVE PERIODONTITIS Sari, Desi Sandra
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 7, No 3 (2010)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Aggressive Periodontitis is a form of periodontitis which is characterized with the presence of deep pocket, rapid loss of connective tissue attachment and alveolar bone. The rapid damage is caused by the dysfunction chemotaxis and phagocytosis from neutrophil.Calprotectin is a major cytosolic protein of neutrophils, monocytes and epithelial cells. The calprotectin level in gingival crevicular fluid is useful marker for evaluation the progression of inflammation in periodontitis disease. In this study, we investigated calprotectin level at different periodontal pocket depth from patient with aggressive periodontitis. This research involved twelve subjects who had been diagnosed as suffered from aggressive periodontitis. Their gingival crevicular fluid was taken from different periodontal pocket depths (slight ≤3mm, moderate 4-6mm, severe ≥ 7 mm), using sterile papperpoints. Level of calprotectin in gingival crevicular fluid were analysed by enzyme-linked immunosorbent assay (ELISA) procedures. The result showed that gingival crevicular fluids calprotectin level increased approximately in proportion to probing depth slight, moderate, and severe (p< 0,05), and showed a significant positive correlation with probing depth (R=0,776, p< 0,05). This research concluded that the calprotectin level in gingival crevicular fluid is positively related to clinical indicators (probing depth) and reflect the degree of gingival inflamation in patient with aggressive periodontitis
STATUS KESEHATAN PERIODONTAL DAN TINGKAT KEBUTUHAN PERAWATAN PASIEN YANG DATANG KE KLINIK PERIODONSIA RSGM UNIVERSITAS JEMBER TAHUN 2011 Ermawati, Tantin; Sari, Desi Sandra; Melok, M
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 9, No 2 (2012)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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The CPITN is a screening procedure for identifying actual and potential problems posed by periodontal disease. The aim of this study was to know periodontal status and periodontal treatment needs from patient that come to RSGM department periodontia faculty of dentistry Jember University in the year 2011. The study population comprised 191 adolescences (10 – 59 years). An observational study was conducted at RSGM of Jember University. The results of the study showed that individuals with a health periodontium is 1.05 %, score (1) with bleeding on probing is 3.14 %. Periodontal pocket (4-5mm) was recorded 17.27 %. The highest periodontal score is scores 2 (calculus) 78.53% of the population need dental scaling. We conclude that prevalence periodontal disease in patients that come to periodontics department is very high and periodontal treatment need with scaling.
The role of probiotic on alveolar bone resorption Sari, Desi Sandra; Meilawaty, Zahara; Amin, M. Nurul
Dental Journal (Majalah Kedokteran Gigi) Vol 44, No 3 (2011): (September 2011)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v44.i3.p117-121

Abstract

Background: Probiotics are microbes derived from the group of lactic acid bacteria that work to maintain the health of hosts. Probiotics can also be used to improve oral health. Periodontal disease is usually marked with gingival inflammation and alveolar bone resorption. Gram negative anaerobic bacteria that play important role in human periodontal disease are Porphyromonas gingivalis. (P. gingivalis). P. gingivalis is a virulent bacteria in vivo or in vitro, and mostly found in subgingival plaque of periodontitis patients. Purpose: This study is aimed to know the role of probiotics to inhibit the resorption of alveolar bone induced with P. gingivalis. Methods: This study used male wistar rats divided into 4 groups. Group I was control group (without treatment); group II was induced with P. gingivalis ATCC 33277 for 5 days; group III was induced with P. gingivalis ATCC 33277 and also injected with probiotics (Lactobacillus casei ATCC 4224) for 5 days simultaneously; and group IV was induced with P. gingivalis ATCC 33277 for 5 days and also injected by probiotics (Lactobacillus casei ATCC 4224) in the next 5 days. After that, the samples were decapitated, taken their alveolar bone, and then were examined by immunohistochemistry to observe osteoclast activity in alveolar bone resorption by using tartrate-resistant acid phosphatase (TRAP) expression. All data were then analyzed statistically. Results: It is known that there were significant differences of TRAP expression among all those treatment groups (p < 0.05). Conclusion: It then can be concluded that probiotics can decrease osteoclast activity in periodontal tissue of wistar rats, so it can inhibit alveolar bone resorption.Latar belakang: Probiotik adalah mikroba dari golongan bakteri asam laktat yang bekerja mempertahankan kesehatan host dan probiotik dapat digunakan untuk meningkatkan kesehatan rongga mulut. Penyakit periodontal ditandai dengan adanya keradangan pada gingiva dan resobsi tulang alveolar. Bakteri Gram negatif anaerob yang sangat berperan dengan penyakit periodontal pada manusia adalah Porphyromonas gingivalis (P. gingivalis). P. gingivalis merupakan bakteri yang virulen, baik diuji secara in vivo maupun in vitro, dan banyak ditemukan pada plak subgingiva penderita periodontitis. Tujuan: Penelitian ini bertujuan untuk mengetahui peran probiotik dalam menghambat resorbsi tulang alveolar yang diinduksi P.gingivalis. Metode: Penelitian ini memakai tikus jenis wistar jantan sebagai sampel dan dibagi menjadi 4 kelompok: Kelompok I yaitu kontrol tanpa perlakuan; kelompok II di induksi P.gingivalis ATCC 33277 selama 5 hari; Kelompok III di induksi P.gingivalis ATCC 33277 ditambah suntikan probiotik (Lactobacillus casei ATCC 4224) selama 5 hari secara bersamaan; dan Kelompok IV di induksi P.gingivalis ATCC 33277 selama 5 hari ditambah suntikan probiotik (Lactobacillus casei ATCC 4224) 5 hari selanjutnya. Setelah itu sampel didekaputasi, diambil tulang alveolar dilakukan pemeriksaan imunohistokimia untuk melihat aktivitas osteoklas dalam resopsi tulang alveolar dengan mendeteksi tartrate-resistant acid phosphatase (TRAP). Data yang dikumpulkan dianalisis secara statistik. Hasil: Terdapat perbedaan yang bermakna antar kelompok untuk ekspresi TRAP (p < 0.05). Kesimpulan: Dapat disimpulkan bahwa probiotik dapat menurunkan aktivitas osteoklas pada wistar tikus yang mengalami periodontitis sehingga dapat mencegah resorbsi tulang alveol.
Detection of aggressive periodontitis by calprotectin expression Sari, Desi Sandra; Suryono, Suryono
Dental Journal (Majalah Kedokteran Gigi) Vol 42, No 4 (2009): (December 2009)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v42.i4.p185-188

Abstract

Background: Calprotectin is a calcium-binding protein expressed by neutrophil, monocytes, gingival keratinocytes, and oral epithelial cells. The concentrations of calprotectin increase in plasma, urine and synovial fluid of patients with inflammatory diseases. This protein is known as a marker for periodontal diseases and is detected in gingival crevicular fluids. Purpose: This study was aimed to investigate the detection of inflammation on the aggressive periodontitis by calprotectin expression. Method: The gingival crevicular fluids were taken from five aggressive periodontitis patients and five healthy subjects by using sterile paper points. Calprotectin expression was analyzed by ELISA technique. Result: The results showed the significant difference in calprotectin expression between subject with aggressive periodontitis and healthy subjects p = 0.002 (p < 0.05). Conclusion: It was concluded that the calprotectin expression on the aggressive periodontitis patients may be useful for evaluation the progression of inflammation in periodontitis.
Cytotoxicity test and characteristics of demineralized dentin matrix scaffolds in adipose-derived mesenchymal stem cells of rats Sari, Desi Sandra; Maduratna, Ernie; Ferdiansyah, F.; Sudiana, I Ketut; Rantam, Fedik Abdul
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 4 (2018): (December 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i4.p194-199

Abstract

Background: Demineralized dentin matrix (DDM) scaffold is a substitute material for the bone contained in human teeth. DDM is a scaffold-derived tooth dentine containing type I collagen and bone morphogenetic protein (BMP). While DDM possesses the ability to perform osteoinductive and osteoconductive roles, a cytotoxicity test of DDM scaffold remains extremely important in evaluating the level of toxicity of a material if cultured in cells. Adipose-derived mesenchymal stem cells (ADMSCs) are multipotent in nature because they contain progenitor cells and have the potential for differentiation via adipogenic, osteogenic and chondrogenic pathways. ADMSCs are also known to have high biocompatibility and the ability to combine with other bone material. Purpose: The purpose of this study was to determine the cytotoxicity and characteristics of DDM scaffolds derived from bovine teeth in the ADMSCs of rats cultured in vitro. Methods: This research constituted an experimental study. ADMSCs were isolated from the inguinal fat of rats. Thereafter, DDM was extracted from bovine teeth and formed 355-710 μm-sized particles. DDM scaffolds were assessed using SEM and the effects of DDM scaffolds on the cell viability of ADMSCs at concentrations of 10%, 50%, and 100% analyzed by means of 3-4,5’dimethylihiazol-2-yl,2.5-di-phenyl-tetrazolium bromide (MTT) assay. The results obtained were then analyzed by an ANOVA to establish the difference between the groups. Results: SEM results showed the diameter sizes of the dental tubulis DDM scaffolds to be approximately 4.429 μm and 7.519 μm. The highest cell viability (97.08%) was found by means of an MTT test to be in ADMSCs at a concentration of 10% compared to those at concentrations of 50% and 100%. Conclusion: In conclusion, DDM scaffold derived from bovine teeth with a particle size of 355-710 μm produces a low cytotoxicity effect on ADMSCs.