Gina Saptiani
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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THE EFFECTIVENESS OF Acanthus Ilicifolius IN PROTECTING TIGER PRAWN (Penaeus monodon F.) FROM Vibrio harveyi INFECTION Saptiani, Gina; Prayitno, Slamet Budi; Anggoro, Sutrisno
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 2 (2012): Volume 15, Number 2, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Sea holly (Achantus ilicifolius) is a mangrove plant that often used by coastal society as a traditional medicine.  It is potential to be developed as the sources of pharmaceutical products. This study aims at assessing the effectiveness of sea holly leaves as antibacterial agent and as an agent to enhance the durability of shrimp against Vibrio harveyi.  First, dried sea holly leaves were extracted with methanol, after that, it was fractionated with silica gel column method using solvent n-hexane, ethyl acetate, and n-butanol.  The treatments given to tiger prawn were crude extract, the fraction of ethyl acetate, and n-butanol, and it was given by immersion.   Next, the challenge test was conducted toward Vibrio harveyi.  As the result, the extract and the leaves of A. ilicifolius possess activities of inhibiting the growth of V. harveyi in vivo, reducing the prevalence of attacks and improving survival of prawn. In general, based on clinical symptoms and pathological anatomy, n-butanol fraction of the A.  ilicifolius leaves possess the best protection, along with ethyl acetate fraction and the crude.
Aktivitas Antibakteri Ekstrak Jeruju (Acanthus ilicifolius) terhadap Pertumbuhan Vibrio harveyi Secara in vitro (ANTIBACTERIAL ACTIVITY OF JERUJU (ANACTHUS ILICIFOLIUS) EXTRACTS ON THE GROWTH OF VIBRIO HARVEYI IN VITRO) Saptiani, Gina; Prayitno, Slamet Budi; Anggoro, Sutrisno
Jurnal Veteriner Vol 13, No 3 (2012)
Publisher : Jurnal Veteriner

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Abstract

The aim of this research was to study the potential of jeruju (Acanthus ilicifolius) extract as anantibacterial for alternative therapy and control of bacterial diseases in prawn nurseries. Ethanol extractionwas prepared from jeruju’s leaves, trunks, fruits, and flowers..Each extract was prepared at differentconcentrations (50 ppm, 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm,900 ppm, and 1000 ppm, respectively) and further tested its antibacterial activity against Vibrio harveyiusing the agar disc diffusion method. The results showed that A. ilicifolius is a potential antibacterial,extract of the leaves seemed to be more effective in inhibit the growth of V. harveyi compared to other partsof the plant.
Pengaruh Penggunaan Ekstrak Daun Jeruju (Acanthus Ilicifolius) Dengan Dosis Berbeda Terhadap Gambaran Darah, Gejala Klinis Dan Kelulushidupan Lele Dumbo (Clarias gariepinus) Yang Diinfeksi Aeromonas hydrophila Ziyadaturrohmah, Siti; Prayitno, Slamet Budi; Sarjito, Sarjito; Hidayati, Nurul; Saptiani, Gina
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Aeromonas hydrophila is a causative agent of Haemoragic Septicemia on Clarias gariepinus. The aim of this research was to investigate the effect of Acanthus ilicifolius leaf extract toward survival rate of C. gariepinus infected by A. hydrophila. Tested fish C. gariepinus 10-12 cm and weight ±25 gr. This reasearch was conducted by 4(four) treatments namely, A (treatment with no leaf ectract A. ilicifolius), B (300 ppm), C (500 ppm), and D (700) 30 minutes immersion. The challenge test was done by injecting 0,1 mL A. hydrophila suspensions with dosage 108 cell/mm3 intra-muscular by experiment fishes. Observation was performed for 5 days after infection such as blood profile, clinical symptoms, and survival rate of C. gariepinus. The results of blood profile after the infection showed that hematokrit of A, B, C, and D treatments was 17,33%, 14,67%, 15,00%, 19,67% respectively. Percentage of the highest erythrocyte was at D treatment 1,79x106 sel/mm3, B 1,72 x106 sel/mm3, A 1,64x106 sel/mm3, C 1,52x106 sel/mm3. Percentage of the highest leuchocyte was at C treatment 5,43x104 sel/mm3, D 3,11x104 sel/mm3, B 2,63x104 sel/mm3, and A 2,32x104 sel/mm3. The result showed that clinical symptoms of A. hydrophila infected fish swam abnormally, injured and haemoragic on the skin along with damaged on the body. Immersion with A. ilicifolius extract leaf past infection indicated that they were not significantly different on C. gariepinus survival rate. Therefore the dosage of A. ilicifolius leaf extract did not sufficient to protect C. gariepinus from A. hydrophila infection.
Toksisitas Produk Ekstraseluler dan Intraseluler Bakteri Pseudomonas sp. pada Ikan Nila (Oreochromis niloticus) (TOXICITY OF EXTRACELLULAR AND INTRACELLULAR PRODUCT OF PSEUDOMONAS SP IN TILAPIA (OREOCHROMIS NILOTICUS) Hardi, Esti Handayani; Pebrianto, Catur Agus; Saptiani, Gina
Jurnal Veteriner Vol 15, No 3 (2014)
Publisher : Jurnal Veteriner

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Abstract

The aim of the research was to investigate the toxicity of extracellular product (ECP) and intracellularproduct (ICP) of Pseudomonas sp. on tilapia. A total of 40 tilapias weighing 15 grams were injected withECP and ICP. The ECP and ICP were harvested from Pseudomonas sp. culture on two kinds of culturemedia and different time of incubation. The Pseudomonas was cultured on trypticase soy agar (TSA) andtrypticase soy broth (TSB) and incubated at 24, 48 and 72 hours. The slurry of the bacteria was centrifugedat 10000 g, for 30 minutes on 4oC to get ECP and in room temperature to get ICP. The supernatant wasfiltered with 0.45 ?m paper mesh. A hundred percent mortality was found in tilapia six hours postinjection with ICP (72 hours) whereas tilapias were injected with ECP caused 60% mortality in 12 hours.The tilapia showed whirling at 24 hour post injected with ECP of Pseudomonas sp which was cultured inTSA for 48 hours incubated. Opacity of the cornea and exopthalmia were occurred at 48 hours postinjection of ECP and ICP which were harvested from both media. Injection of ICP caused pathologychanges on internal organ of fish i.e. pale appearance of spleen and liver. In conclusion, the ECP and ICPwere a virulence factors of Pseudomonas sp. and the ICP seem more pathogenic and caused mortality thanECP. Both culture media and time of incubation influence of ECP and ICP production. The ECP and ICPwhich were harvested from Pseudomonas sp incubate for 24-48 hour more virulent than 72 hour.
Ekstrak Daun Pepaya dan Kangkung untuk Meningkatkan Daya Tetas Telur dan Kelangsungan Hidup Larva Lele (EXTRACTS OF CARICA PAPAYA AND IPOMOEA AQUATICA FOR IMPROVING EGG HATCHABILITY AND LARVAL VIABILITY OF CATFISH) Saptiani, Gina; Hardi, Esti Handayani; Pebrianto, Catur Agus; Agustina, Agustina
Jurnal Veteriner Vol 17, No 2 (2016)
Publisher : Jurnal Veteriner

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Abstract

This research was aimed to investigate the potential use of leaf extract of Carica papaya and Ipomoeaaquatica lto improve egg hatchability and larval viability of catfish. Dried leaves of Carica papaya andIpomoea aquatica were macerated and extracted in water and ethanol. Eggs and larvae were tested in theaquarium size of 10 L with a a diameter of 28 cm. The extracts in concentration 600, 800 and 1.000 ppmwere tested on the egg hatchability of catfish with immersion method, and challed with Aeromonashydrophyla, Pseudomonas sp., and Saprolegnia spp. The extracts in concentration 800 and 1.000 ppm weretested on the larval viability with immersion method, and challed with pathogens. Water or ethanolextract of Carica papaya and Ipomoea aquatica can improve egg hatchability 67±8% until 90±6% andlarval viability of catfish 77±0,5 until 90±9%. Eight hundred ppm ethanol extract of Carica papaya has thebest egg hatchability and 1000 ppm can improve larval viability of catfish.
POTENSI ANTIBAKTERI EKSTRAK DAUN JERUJU (Acanthus ilicifolius) TERHADAP Vibrio harveyi SECARA IN VITRO Saptiani, Gina; Prayitno, Slamet Budi; Anggoro, Sutrisno
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Untuk mengetahui potensi antibakteri dan konsentrasi optimal jeruju (Acanthus ilicifolius), dilakukan uji daya hambat ekstrak dan fraksi daun terhadap pertumbuhan Vibrio harveyi, bakteri penyebab penyakit pada udang. Daun yang sudah dikeringanginkan diekstraksi dengan metanol, sehingga didapatkan ekstrak kasar (crude). Sebagian dari ekstrak kasar dilakukan fraksinasi dengan metode kolom silika gel dengan pelarut n-heksan, etil asetat, n-butanol, etanol, dan metanol. Uji daya hambat dilakukan dengan agar disc diffusion method. Perlakuan yang diberikan adalah ekstrak kasar, fraksi n-heksan, etil asetat, n-butanol, etanol, dan metanol yang masing-masing konsentrasinya 50-1000 ppm, yang diberikan pada kultur V. harveyi pada media Triptic Soy Agar (TSA). Fraksi etil asetat menunjukkan daya hambat terbaik (12 mm), diikuti ekstrak (11,33 mm), dan fraksi n-butanol (11 mm).
Effect of Fish Transportation on immunological response and Bacterial Diseases in Anabas Testudineus Fish Hardi, Esti Handayani; Asmir, Asmir; Pebrianto, Catur Agus; Saptiani, Gina; Agustina, Agustina
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

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Abstract

This study was conducted to determine the condition of immunological response of and the presence of bacterial diseases in Anabas Testudineus fish was transported from pond culture towards Segiri Market. Transport system carried out by the fishmonger supposed to influence the health conditions of fish as well as the presence of bacteria on the fish's body. The sample was 30 fish from Muara Kaman pond and Markets Segiri as a fish marketing. Some parameters were observed among others: changes in swimming behavior, pathology anatomy organs, aemotology and inventory of bacterial diseases. The results showed that the samples of fish from the pond aquaculture generally have normal conditions of organ anatomy, symptom behavior swim also normally, otherwise fish from marketed in the Market Segiri, as much as 60% of fish have abnormal behavior of swimming, 73% have pathology on external anatomy and 53% fish have abnormalities in organs. Results of the isolation and identification of bacterial disease found that there are 5 types of bacteria Aeromonas, Pseudomonas, Staphyllococcus, Streptococcus and Basillus, and only bacteria Staphyllococcus and Basillus not found in fish pond aquaculture origin. The health condition of the fish pond aquaculture origin betook better, judging from the hemoglobin, hematocrit, total leukocyte and total erythrocyte that is within the normal range. While the blood picture fish from Segiri market is generally higher in all parameters that indicate health problems. It can be concluded that the transport system carried out by the wet etching fishmonger effect on the occurrence of abnormalities in behavioral symptoms, anatomic pathology outer and inner organs, the presence of bacterial disease and the decline in fish health conditions.Keywords : Betok fish, bacterial, transportation