Articles

The Role of Surfactant Micelles on the Partitioning of Antioxidant and the Oxidative Stability of Oil-in-Water Emulsion

Jurnal Teknologi Dan Industri Pangan Vol 19, No 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Lipid oxidation system in which the fat is dispersed as emulsion droplets is still not well understood, although a large number of food exist partially or entirely in the form of emulsions. Therefore, this study was intended to examine how surfactant micelles influence the partitioning of antioxidant and hydroperoxides and how it alter the oxidative stability of oil-in-water emulsion. To determine the ability of surfactant micelles upon the partitioning of antioxidant in oil-in-water emulsion, this type of emulsion, containing quercetin at 0, 25, 50, 75 and 100 ppm, were prepared with polyoxyethylene 100 stearyl ether (Brij 700) or polyoxyethylene sorbitan monolaurate (Tween 20) with acetate or phosphate buffer (pH 3.0 or 7.0). Structurally Birj 700 has 5 times longer polyoxyethylene groups than Tween 20. After preparation of the emulsion, surfactant micelles (0-2%) were incorporated into the continuous phase to give a final lipid concentration of 5%. Lipid oxidation rates, as determined by the formation of lipid hydroperoxides, decreased with increasing quercetin concentrations. At pH 3, the peroxide value was higher than that at pH 7. Brij 70 decreased production of lipid hydroperoxides from palm oil-in-water-emulsions compared to the emulsions stabilized by Tween 20. the result showed that solubilization of quercetin into the aqueous into the aqueous phase by Brij or Tween micelles did not alter the oxidative stability of palm oil-in-water emulsion, suggesting that surfactant micelle influenced the oxidation rate by mechanism other than antioxidant solubilization. Key word : Quercetin, surfactant micelles, and oxidative stability

PENGARUH KONSENTRASI PATI AREN DAN MINYAK SAWIT TERHADAP SIFAT FISIK DAN MEKANIK EDIBLE FILM

AGROLAND Vol 17, No 1 (2010)
Publisher : AGROLAND

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Abstract

The general aims of the research were to develop the utilization of palm sugar starch and to identify the optimal condition for processing edible film. The specific objectives of the research were to determine the palm sugar  starch and palm oil concentrations for processing the edible film from natural palm sugar starch. Processing edible film from natural palm sugar starch was done by making palm sugar starch suspensions  with various treatment concentrations of 1.6%, 2.0%, 2.4%, and 2.8% (w/v).  Whereas the palm oil concentrations were 10%, 20%, and 30% (w/w).  Parameters observed were physical, and mechanical characteristics including thickness, water vapor transmission rate (WVTR), tensile strength, and elongation. All treatments were replicated three times.  Statistical data analysis used Software Statistical Product and Service Solution (SPSS) version 13th with One Way Anova and Univariate Analysis of Variance at 5% significant level in conjunction with Duncan’s method for mean comparisons. The research result showed that the optimum condition of  palm sugar starch concentration for edible processing film was found at 2.8% (w/v) with mechanical and physical characteristics of 0.092 mm thickness, 3.737 g m-2 h-1 WVTR, 23.877 MPa tensile strength, and 1.727% elongation. Whilst the optimum condition for the palm oil concentration was 10% (w/w) with mechanical and physical characteristics of 0.097 mm thickness, 16.623 g m-2 h-1 WVTR, 21.847 MPa tensile strength, and 1.507% elongation.

The Effect of Size Reduction and Preparation Duration on The Antioxidant Activity of White Saffron (Curcuma mangga Val.)

Journal of Food and Pharmaceutical Sciences Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
Publisher : Gadjah Mada University

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Abstract

Food processing is usually conducted through preparation stages such as peeling, slicing, or cutting. The purpose of this research was to determine the correlation between the antioxidant activity and the total phenolic compounds of white saffron as affected by different level of size reduction and the duration of rhizome preparation at room temperature. White saffron rhizomes were peeled, washed, and cut into 1x1x1 cm3. The whole white saffron was sliced diagonally at thickness of 4 x 2 mm, and grated. The antioxidant activity of the white saffron was determined by radical scavenging activity using 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical and by ferric reducing antioxidant power FRAP) method. The total phenol and total flavonoid contents were also determined at 2, 4, and 6 hours, respectively. The result showed that the antioxidant activities, total phenolic and flavonoid contents due to the preparation of whole white saffron for 6 hour are not statistically different compared to those of fresh white saffron. The preparation of white saffron sliced at 2 mm and grated for 2 hour showed a significant decrease in the antioxidant activities, compared to those of fresh white saffron.Keywords: white saffron, preparation duration, antioxidant activity, total phenol

Mechanism and Kinetics of Quenching of Quercetin on Photosensitizing Effect of Synthetic Food Colorant Erythosine in Palm Oil Oxidation

Jurnal Teknologi Dan Industri Pangan Vol 16, No 2 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The effect 0, 200, 400, 600, 800 and 1000 ppm (wt/vol) quercetin on the singlet oxygen oxidation of palm oil in methylene chloride containing 100 ppm erythrosine, were studied during storage under 400 lux fluorecent light for 5 h by measuring peroxide value. Steady-state kinetic approximation was used to determine the quenching mechanism and quenching rate constant of quercetin in the erythrosine-sensitized photo oxidation of palm oil. Erythrosine greatly increased the As photo oxidation of palm oil, as was expected. Quercetin was extremely effective at minimizing erythrosine-sensitized photo oxidation of palm oil. As the concentration of quercetin increased from 0 to 200, 400, 600, 800 and 1000 ppm, the peroxide value of palm oils decreased significantly (p

Mechanism and Kinetic of Antiphotooxidation of Bixin in Metil Linoleat System

Jurnal Teknologi Dan Industri Pangan Vol 17, No 3 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Bixin belongs to the carotenoid group, present in Bixa orellana tree. It has conjugated double bonds which plays a role as an antiphotooxidant. The objectives of this study were to analyze the quenching mechanisms and kinetics of bixin in photosensitized oxidation of methyl linoleate. The quenching mechanism and kinetic of bixin were studied by the steady-state kinetic method. Samples of 0, 0.25 x 10-5, 0.5 x 10-5, 0.75 x 10-5, 1.0 x 10-5, 2.0 x 10-5 and 3.0 x 10-5 M bixin were prepared in ethanol that also contained 0.03, 0.06, 0.09 and 0.12 M methyl linoleate and 11.36 x 10-6 M of erythrosine stored under fluorescent light (4000 lux) at room temperature for 2 hours. The extent of photosensitized oxidation of methyl linoleate was expressed as peroxide value. The steady-state kinetic study indicated that bixin quenched singlet oxygen and exhibited triplet sensitizer quenching mechanism (to minimize the erythrosine photosensitized oxidation of methyl linoleate). The total quenching rate constant of bixin was 7.7 x 109 M-1S-1. Key words: bixin, quencher, erythrosine, singlet oxygen

Chemical, Physicochemical and Sensory Characterization of Jam and Jelly Made from Pumpkin Strands (Cucurbita maxima)

Jurnal Teknologi Dan Industri Pangan Vol 17, No 2 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objective of this research was to examine the contents of pectin,???carotene and sugars of the pumpkin strands, and to evaluate the effects of sugar addition and pH on the chemical, physical, and sensory characteristics of jam and jelly made from pumpkin strands. The preparation of jam and jelly were conducted with sugar addition of 55%, 60%, and 65% and pH values of 3.0, 3.2, and 3.4. The products were analyzed for moisture, sugar,???carotene, and soluble solid, and were evaluated for firmness and sensory properties.The results showed that the moisture content of jelly was between 26.36 - 35.27% and that of the jam between 39.53 - 45.67%. Beta-carotene of jelly was between 241.79 - 404.42 RE/100g (db) and that of jam was between 235.58 - 487.51 RE/100g (db). The decreasing of pH and increasing of sugar addition tended to increase the content of reducing sugars and soluble solid of the products. Jelly could be made from pumpkin strands by addition of 55% or 60% sugar, and the firmness increased by decreasing of pH value. There were no significance differences between the treatments for color, flavor and overall preference score. The addition of 60% sugar and pH of 3.2 was the best condition for making jam and jelly from pumpkin strands. Key words: Pumpkin strands, jam, jelly.

Extrusion product made from sweet potato

Jurnal Teknologi Dan Industri Pangan Vol 18, No 1 (2007): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objective of the research was to develop a method to prepare extruted product from sweet potato and rice flour with addition of karabenguk as the protein source. The ratio of sweet potato and rice flour were 90:10; 75:25; and 55:45, and the addition of korobenguk flour varied from 0.5;1.0to 1.5 % (on the mixture basis). The first step was to determine the optimum condition for extrusion process. The extruded product was evaluated for its chemical, physical, and sensory properties. The results showed that the extruded products can be produced from sweet potato and rice flour in all ratios experimented. The addition of korobenguk flour appeared to increase protein, fat, and ash contents but decrease the extension degree the extension degree, crispness, and water absorption index. Based on the sensory evaluation, the most preferred product was that prepared from 55% sweet potato flour, 45% rice flour, and addition of 0.5% korobenguk flour (on the basis of the dough) with moisture content of 15 %. Key words: sweet potato, extrusion product, korobenguk

PENGGUNAAN RESPONSE SURFACE METHODOLOGY UNTUK OPTIMASI PROSES DEKAFEINASI MENGGUNAKAN KITOSAN DARI KULIT UDANG [The Use of Response Surface Methodology in Decaffeination Process with Chitosan]

Jurnal Teknologi Dan Industri Pangan Vol 12, No 1 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

PENGGUNAAN RESPONSE SURFACE METHODOLOGY UNTUK OPTIMASI PROSES DEKAFEINASI MENGGUNAKAN KITOSAN DARI KULIT UDANG [The Use of Response Surface Methodology in Decaffeination Process with Chitosan] Umar Santoso 1) , Novia Tabahana 2) , Henny Krissetiana H 3) , dan Suhardi 1) 1) Staf Pengajar Fakultas Teknologi Pertanian - UGM, Yogyakarta 2) Alumnus Fakultas Teknologi Pertanian -UGM, Yogyakarta 3) Staf Pengajar Fakultas Teknologi Pertanian - INTAN, Yogyakarta ABSTRACT The objective of the present study was to determine the optimum condition of decaffeination process with chitosan in a model system using Response Surface Methodology. A 1000ppm caffeine solution was mixed with chitosan in varried concentrations, temperatures and process times.  After filtration, caffeine in the filtrate was determined. The lower caffeine in the filtrate the more effective the decaffeination process. Result of  the experiment showed that among  chitosan concentrations of  50, 60, 70, 80, 90, and 100 mg per 100 ml caffeine solution, the concentration of 70mg was the most effective. Among temperatures applied of 28, 40, 60, 80, 90, and 100oC, the most effective was of 90oC. And among the process times of 15, 30, 60, and 90 minutes, 15 minutes was the most effective. Result of optimatization using RSM showed that  the optimum condition of decaffeination process were concentration of chitosan of 69,52mg, temperature of 89,71oC, and process time of 14,88 minutes. Under this condition the process diminished 79,56% of caffeine from the model system.

Pengaruh Pemanasan Kelapa Parut Dan Teknik Pengunduhan Terhadap Rendemen Dan Mutu Virgin Coconut Oil (VCO)

Jurnal Keteknikan Pertanian Vol 22, No 2 (2008): Jurnal Keteknikan Pertanian
Publisher : PERTETA

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Abstract

Virgin Coconut Oil (VCO) was produced from green variety ofnon-hybride coconut type. Grated coconut was heated at 500, 70°, and 90°C for 20, 30 and 40 minutes, respectively. Harvesting technique of VCO were done by conventional, utilization of stainless steel screen, and filter paper laminated stainless steel screen methods, respectively. Produced VCO was subsequently analyzed their productivity and quality parameters consisting of moisture content, acid number, free fatty acid (FFA), peroxide number, iodine number, saponification number, clearness and fatty acid profile of VCO. Statistical analysis was also conducted by using Analysis of Variance and Duncan Multiple Range Test with 5% significancy.The results showed that heating ofgrated coconut at 900C for 40 minutes produced 29.65 ±0.38% VCO, and it was the highest productivity.-Heating temperature ofgrated coconut and with any harvesting technique didnt statistitically have significant effect on productivity and quality of VCO. While, combination of heating temperature at 90°C and it was followed by harvesting technique using stainless steel screen produced the highest VCO i.e. 31.54 ± 0.58%. The best quality of VCO was characterized by 0.09 ± 0.01% moisture content, 0.20 ± 0.02 mg KOH/g oil of acid number, 0.12 ± 0.01% free fatty acid (FFA), 0.37 ± 0.01 meq/kg oil ofperoxide number, 5. 11 ± 0.01 g iod/100 g oil ofiodine number, 244. 12 ± 0.01 mg KOH/g oil saponification number and the VCO was clear and transparant. Fatty acid profile of selected VCO was dominated by medium chain fatty acid (C12:0) particularly lauric acid i.e. 50.26% and followed by 8.65% caprylic and 5,73% capric acids, respectively. "The quality parameters of VCO was able to meet the quality standard of VCO that was issued by APCC (Asia Pacific Coconut Community) quality grade.Keywords: Grated coconut, heating, harvesting technique and VCO.Diterima: 19 Apri 2008; Disetujui:27 Juli 2008

POTENSI KENTANG HITAM DALAM MEREDUKSI STRES OKSIDATIF DAN MENGHAMBAT PROLIFERASI SEL KANKER PAYUDARA MCF-7 [Potential of Black Potato in Reducing Oxidative Stress and Inhibiting the Proliferation of Breast Cancer Cells MCF-7]

Jurnal Teknologi Dan Industri Pangan Vol 24, No 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The ethanolic extracts of flesh and peel of Coleus tuberosus were evaluated for its ability to reduce oxidative stress using cellular antioxidant activity testing on MCF-7 cells based on oxidation of 2.7-dichlorofluoresce diacetate (DCFH). Meanwhile, the antiproliferative activities was evaluated based on 3-(4.5-Dimethylthiazol-2) -2.5-diphenyltetrazolium bromide (MTT) assay in MCF-7 cells. The results showed that the ethanolic extract of flesh and peel have the ability to reduce oxidative stress and inhibit the proliferation of breast cancer cells MCF-7. The optimum concentration of ethanol extracts of peel in reducing oxidative stress was 200 μg/mL, while that of flesh was 400 μg/mL. Inhibitory concentration (IC50) of cell proliferation of MCF-7 by peel extract was 698.23±1.61 (µg/mL), and flesh extracts was 829.86±5.73 (µg/mL). The ability to reduce and inhibit the proliferation showed a dose dependent manner. There is a high correlation between cellular antioxidant and antiproliferation. The correlation of cellular antioxidant activity of the ethanolic extract of flesh Coleus tuberosus and antiproliferation was 0.93, while the correlation between cellular antioxidant activity ofthe ethanolicextract ofthe peel and anti-proliferation was 0.98. These results suggest that the ethanolic extract of flesh and peel of Coleus tuberosus can be used as a source of natural antioxidants and anti-proliferation.